BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0059.p (445 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0378_2 [R] COG0456 Acetyltransferases 229 5e-60 CAC1847_2 [J] COG0539 Ribosomal protein S1 212 1e-54 all0136 [J] COG0539 Ribosomal protein S1 97 7e-20 slr1356 [J] COG0539 Ribosomal protein S1 96 1e-19 slr1984 [J] COG0539 Ribosomal protein S1 88 3e-17 >ML0378_2 [R] COG0456 Acetyltransferases Length = 155 Score = 229 bits (585), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 0/389 (0%), Positives = 0/389 (0%), Gaps = 13/389 (3%) Query: 62 DTVKGTIVKIEDNQAYVSI-GYKYDGVIPIRELSSVQLDNXXXXXXXXXXXXCKVVSIND 120 + + + Sbjct: 18 VDRCAQLESQFFDGDNPWPAAAFDRELANSYNCYVGARTADTLVGYAGITRLGHTPPFEY 77 Query: 121 NKES-----LVLSKRAVDSEKSWEDLEKYFASQEAFEVTVADVVKGGLVADV-GARGFIP 174 + + S + D + G Sbjct: 78 EVHTIAVDPAYRGRGVGRRLLGELLDFA--GSGAIYLEVRTDNETAIALYRSVGFERIGL 135 Query: 175 ASMVERHFVEDFSDYKGRTLRVKVKELDRENSKVILSAKEVLEEEFEANKQKIMSELSEG 234 Sbjct: 136 195 Query: 235 QIIEGTVQRLTQFGAFXXXXXXXXLVHVSEIAWNHVEKPSDXXXXXXXXXXXXXXXXXXX 294 Sbjct: 196 255 Query: 295 GKISLSIKAAAPGPWDSAAGKINIGDVVTGEVKRLVNFGAFVELLPGVEGLVHISQISHK 354 Sbjct: 256 315 Query: 355 HIGTPHEVLKEGQEVQVKVLDFNPSEKRVSLSIKXXXXXXXXXXXXXXSNN--RDRAPKE 412 Sbjct: 316 375 Query: 413 VLN--NPNVSLSNEGLSFTLAERFGDKLD 439 Sbjct: 376 404 >CAC1847_2 [J] COG0539 Ribosomal protein S1 Length = 372 Score = 212 bits (539), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 118/331 (35%), Positives = 190/331 (57%), Gaps = 4/331 (1%) Query: 61 GDTVKGTIVKIEDNQAYVSIGYKYDGVIPIRELSSVQLDNXXXXXXXXXXXXCKVVSIND 120 G +V G I+++ + + +++I YK DGVIP E+ D K++ + D Sbjct: 39 GASVTGEIIQVSEKEVFLNINYKRDGVIPKSEIDDDGKD-LKELFTVGDKIVAKIIKLKD 97 Query: 121 NKESLVLSKRAVDSEKSWEDLEKYFASQEAFEVTVADVVKGGLVADV-GARGFIPASMVE 179 +VLS + + E+ ++++++ F ++ V V + VKGG++A G R FIPAS VE Sbjct: 98 ADNYVVLSVKELQREQGYKEIKEAFENKTTLNVVVKEDVKGGIIASYKGIRIFIPASHVE 157 Query: 180 RHFVEDFSDYKGRTLRVKVKELD--RENSKVILSAKEVLEEEFEANKQKIMSELSEGQII 237 V++ +Y G++ V + E + +K++ S + +L +E E ++ + ++L EGQ++ Sbjct: 158 LFHVDNLKEYIGKSFDVAIIEYSTKKRQTKIVASRRALLSKEKEKVEETVWNKLEEGQVV 217 Query: 238 EGTVQRLTQFGAFXXXXXXXXLVHVSEIAWNHVEKPSDXXXXXXXXXXXXXXXXXXXGKI 297 EG V+RLT FGAF L+HVSEI+W VEKP+D K+ Sbjct: 218 EGEVKRLTDFGAFVEIEGVDGLLHVSEISWGRVEKPADVLKIGDKIKVYVLSVDKENKKL 277 Query: 298 SLSIKAAAPGPWDSAAGKINIGDVVTGEVKRLVNFGAFVELLPGVEGLVHISQISHKHIG 357 SLS+K PW++ K +G VV G+V R +FGAFV+L PGV+GLVHIS+ISHK I Sbjct: 278 SLSVKKLTENPWNNVEEKYPVGSVVLGKVIRFADFGAFVKLEPGVDGLVHISEISHKRIA 337 Query: 358 TPHEVLKEGQEVQVKVLDFNPSEKRVSLSIK 388 P + L G+E++ K+L+ + EK++ LSI+ Sbjct: 338 KPSDALNVGEEIKAKILEVSSEEKKIGLSIR 368 >all0136 [J] COG0539 Ribosomal protein S1 Length = 343 Score = 96.7 bits (239), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 69/251 (27%), Positives = 113/251 (45%), Gaps = 10/251 (3%) Query: 61 GDTVKGTIVKIEDNQAYVSIGYKYDGVIPIRELSSVQLDNXXXXXXXXXXXXCKVVSIND 120 GD V GT+ IE A + IG K IPI+E+S ++D +++ + Sbjct: 32 GDVVPGTVFSIEPRGALIDIGAKTAAYIPIQEMSINRVDAPEEVLQSNETREFFILTDEN 91 Query: 121 NKESLVLSKRAVDSEKSWEDLEKYFASQEAFEVTVADVVKGGLVADV-GARGFIPASMVE 179 L LS R ++ ++WE + + A V +GG + + G RGFIP S + Sbjct: 92 EDGQLTLSIRRIEYMRAWERVRQLQAEDATVRSGVFATNRGGALVRIEGLRGFIPGSHIS 151 Query: 180 RHFVEDFSDYKGRTLRVKVKELDRENSKVILSAKEVLEEEFEANKQKIMSELSEGQIIEG 239 ++ + G L +K E+D E ++++LS + L E + M+ L G+++ G Sbjct: 152 TRKPKE--ELVGEELPLKFLEVDEERNRLVLSHRRALVE-------RKMNRLEVGEVVIG 202 Query: 240 TVQRLTQFGAFXXXXXXXXLVHVSEIAWNHVEKPSDXXXXXXXXXXXXXXXXXXXGKISL 299 TV+ + +GAF L+H+SEI+ H++ P G+ISL Sbjct: 203 TVRGIKPYGAFIDIGGVSGLLHISEISHEHIDTPHSVFNVNDEVKVMIIDLDAERGRISL 262 Query: 300 SIKAAAPGPWD 310 S K P P D Sbjct: 263 STKQLEPEPGD 273 Score = 70.9 bits (172), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 33/74 (44%), Positives = 51/74 (68%), Gaps = 1/74 (1%) Query: 315 KINIGDVVTGEVKRLVNFGAFVELLPGVEGLVHISQISHKHIGTPHEVLKEGQEVQVKVL 374 ++ +G+VV G V+ + +GAF+++ GV GL+HIS+ISH+HI TPH V EV+V ++ Sbjct: 193 RLEVGEVVIGTVRGIKPYGAFIDI-GGVSGLLHISEISHEHIDTPHSVFNVNDEVKVMII 251 Query: 375 DFNPSEKRVSLSIK 388 D + R+SLS K Sbjct: 252 DLDAERGRISLSTK 265 >slr1356 [J] COG0539 Ribosomal protein S1 Length = 328 Score = 95.9 bits (237), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/252 (26%), Positives = 112/252 (44%), Gaps = 10/252 (3%) Query: 58 LKKGDTVKGTIVKIEDNQAYVSIGYKYDGVIPIRELSSVQLDNXXXXXXXXXXXXCKVVS 117 GD V GT+ +E A + IG K IPI+E+S ++D+ +++ Sbjct: 28 FSPGDIVAGTVFSMESRGALIDIGAKTAAYIPIQEMSINRVDDPEEVLQPNETREFFILT 87 Query: 118 INDNKESLVLSKRAVDSEKSWEDLEKYFASQEAFEVTVADVVKGGLVADV-GARGFIPAS 176 + L LS R ++ ++WE + + A V +GG + + G RGFIP S Sbjct: 88 DENEDGQLTLSIRRIEYMRAWERVRQLQAEDATVRSNVFATNRGGALVRIEGLRGFIPGS 147 Query: 177 MVERHFVEDFSDYKGRTLRVKVKELDRENSKVILSAKEVLEEEFEANKQKIMSELSEGQI 236 + E D G L +K E+D E ++++LS + L E + M+ L Q+ Sbjct: 148 HISAR--EAKEDLVGEDLPLKFLEVDEERNRLVLSHRRALVE-------RKMNGLEVAQV 198 Query: 237 IEGTVQRLTQFGAFXXXXXXXXLVHVSEIAWNHVEKPSDXXXXXXXXXXXXXXXXXXXGK 296 + G+V+ + +GAF L+H+SEI+ +H++ P G+ Sbjct: 199 VVGSVRGIKPYGAFIDIGGVSGLLHISEISHDHIDTPHSVFNVNDEIKVMIIDLDAERGR 258 Query: 297 ISLSIKAAAPGP 308 ISLS K P P Sbjct: 259 ISLSTKQLEPEP 270 Score = 67.4 bits (163), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 31/73 (42%), Positives = 47/73 (64%), Gaps = 1/73 (1%) Query: 316 INIGDVVTGEVKRLVNFGAFVELLPGVEGLVHISQISHKHIGTPHEVLKEGQEVQVKVLD 375 + + VV G V+ + +GAF+++ GV GL+HIS+ISH HI TPH V E++V ++D Sbjct: 193 LEVAQVVVGSVRGIKPYGAFIDI-GGVSGLLHISEISHDHIDTPHSVFNVNDEIKVMIID 251 Query: 376 FNPSEKRVSLSIK 388 + R+SLS K Sbjct: 252 LDAERGRISLSTK 264 >slr1984 [J] COG0539 Ribosomal protein S1 Length = 305 Score = 87.8 bits (216), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 59/214 (27%), Positives = 105/214 (49%), Gaps = 10/214 (4%) Query: 59 KKGDTVKGTIVKIEDNQAYVSIGYKYDGVIPIRELSSVQLDNXXXXXXXXXXXXCKVVSI 118 +KG TV G I + + YV G K G +P++EL V S Sbjct: 27 EKGQTVHGKICQHANEGVYVDFGGKSPGFVPVQELGLRPHAEIEDSFPLDSAWDFLVTSE 86 Query: 119 NDNKESLVLSKRAVDSEKSWEDLEKYFASQEAFEVTVADVVKGGLVADV-GARGFIPASM 177 +++ + LS+R + ++SWE+L + S + E+ V KGG+V DV G RGFIP S Sbjct: 87 QNDEGQVRLSRRQLQIQQSWENLAELEESGKTLEMVVTGTNKGGVVGDVEGLRGFIPRSH 146 Query: 178 VERHFVEDFSDYKGRTLRVKVKELDRENSKVILSAKEVLEEEFEANKQKIMSELSEGQII 237 + ++ G+ L+ + E +++N+K++L+ + + + E M +++ G I Sbjct: 147 LMHK--DNMDALVGQVLKAHILEANQDNNKLVLTQRRIQQAE-------SMGKIAAGNIY 197 Query: 238 EGTVQRLTQFGAFXXXXXXXXLVHVSEIAWNHVE 271 EG V ++ +G F L+HVS+++ V+ Sbjct: 198 EGKVAKIQPYGVFVEIEGVTGLLHVSQVSGTRVD 231 Score = 50.8 bits (120), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 26/77 (33%), Positives = 47/77 (61%), Gaps = 1/77 (1%) Query: 312 AAGKINIGDVVTGEVKRLVNFGAFVELLPGVEGLVHISQISHKHIGTPHEVLKEGQEVQV 371 + GKI G++ G+V ++ +G FVE+ GV GL+H+SQ+S + + + + GQ + V Sbjct: 187 SMGKIAAGNIYEGKVAKIQPYGVFVEI-EGVTGLLHVSQVSGTRVDSLNTLFAFGQAISV 245 Query: 372 KVLDFNPSEKRVSLSIK 388 V + + + R+SLS + Sbjct: 246 YVQEIDEYKNRISLSTR 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.131 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,848,152 Number of extensions: 898398 Number of successful extensions: 1405 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1374 Number of HSP's successfully gapped: 21 Length of query: 445 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 335 Effective length of database: 25,691,691 Effective search space: 8606716485 Effective search space used: 8606716485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 117 (49.7 bits)