BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0058.p (375 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1711 [R] COG1160 Predicted GTPases 361 1e-99 TM1446 [R] COG1160 Predicted GTPases 232 7e-61 CAC1651 [R] COG1160 Predicted GTPases 65 2e-10 PH0344 [R] COG1100 GTPase SAR1 and related small G proteins 59 2e-08 SMc00581 [O] COG0760 Parvulin-like peptidyl-prolyl isomerase 57 6e-08 >CAC1711 [R] COG1160 Predicted GTPases Length = 438 Score = 361 bits (926), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 178/362 (49%), Positives = 249/362 (68%) Query: 1 LAIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVI 60 ++IVED PG+TRDRIY S+W GK F++IDTGGIE + D IL +R QA++AIE +DVI Sbjct: 28 VSIVEDTPGVTRDRIYAESEWVGKKFTIIDTGGIEPENNDIILTQMRRQAQIAIEMSDVI 87 Query: 61 VFMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIYEFYSLGIGDPXXXXX 120 +FM + K GLT+ D EVA +L +S KP+VL +NK+D +IYEFY+LGIGDP Sbjct: 88 IFMVDGKQGLTDTDNEVAIMLRKSKKPIVLVVNKIDKNVEENNIYEFYNLGIGDPVSISS 147 Query: 121 XXXXXXXDLLDAVTERLPEPVEEEYDDDIIRVALIGRPNVGKSSLVNAILGEERVIVSDV 180 D+LD V + E+E +++ I++A +G+PNVGKSSL N ILGEERVIVSD+ Sbjct: 148 SQGLGIGDMLDEVVNKFKSEGEDEEEEEYIKIAFVGKPNVGKSSLTNRILGEERVIVSDI 207 Query: 181 AGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVVIN 240 GTTRDAID+ E D + VLIDTAG+R++ ++ E E+YS +R M AIER DV ++++ Sbjct: 208 PGTTRDAIDSFLETDFGKLVLIDTAGLRRKSRIKEEIERYSAVRTMAAIERCDVCTLILD 267 Query: 241 GEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVV 300 E I +QD+ I GYAH+ KA + +VNKWD +EKDDKTM+NF+ + F FM+YAP + Sbjct: 268 ATEPISEQDERIIGYAHENNKAILVIVNKWDLIEKDDKTMENFKKNLEMKFSFMAYAPFL 327 Query: 301 FLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPPTDKGRRLRXXXLR 360 F+SA T QR+ K+L ++ ++ RI T ++NDV+S+AV + PP +RL+ + Sbjct: 328 FISAKTGQRVHKVLSEIKKCYDNYSKRIATGVLNDVISNAVLMKEPPVVAFKRLKIFYVT 387 Query: 361 DT 362 T Sbjct: 388 QT 389 Score = 78.6 bits (192), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 46/122 (37%), Positives = 69/122 (56%), Gaps = 6/122 (4%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 V ++GRPNVGKS+L N + G+ IV D G TRD I E G+++ +IDT G+ Sbjct: 6 VTIVGRPNVGKSTLFNKLAGKRVSIVEDTPGVTRDRIYAESEWVGKKFTIIDTGGIEPEN 65 Query: 212 KVYETTEKYSVMR--AMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNK 269 T+ MR A AIE +DV++ +++G++G+ D D +A + K + VVNK Sbjct: 66 NDIILTQ----MRRQAQIAIEMSDVIIFMVDGKQGLTDTDNEVAIMLRKSKKPIVLVVNK 121 Query: 270 WD 271 D Sbjct: 122 ID 123 >TM1446 [R] COG1160 Predicted GTPases Length = 439 Score = 232 bits (592), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 127/353 (35%), Positives = 204/353 (57%), Gaps = 7/353 (1%) Query: 2 AIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVIV 61 AIVED+ G+TRD + +W GK+F ++DT G+ + +D I + ++ I EAD+++ Sbjct: 27 AIVEDEEGVTRDPVQDTVEWYGKTFKLVDTCGVFDNPQDIISQKMKEVTLNMIREADLVL 86 Query: 62 FMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIY--EFYSLGIGDPXXXX 119 F+ + K G+T DE +A L +S +L NK +N++ E E YSLG G+P Sbjct: 87 FVVDGKRGITKEDESLADFLRKSTVDTILVANKAENLREFEREVKPELYSLGFGEPIPVS 146 Query: 120 XXXXXXXXDLLDAVTERLPEP---VEEEYD-DDIIRVALIGRPNVGKSSLVNAILGEERV 175 LL+ + ++L E +E + + D I+VA++GRPNVGKS+L NAIL +ER Sbjct: 147 AEHNINLDTLLETIIKKLEEKGLDLESKPEITDAIKVAIVGRPNVGKSTLFNAILNKERA 206 Query: 176 IVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVY-ETTEKYSVMRAMRAIERADV 234 +VS + GTTRD +D DG++YV +DTAG+R++ +V T EKYS R + +IE+ADV Sbjct: 207 LVSPIPGTTRDPVDDEVFIDGRKYVFVDTAGLRRKSRVEPRTVEKYSNYRVVDSIEKADV 266 Query: 235 VLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFM 294 V++V++ +GI QD+ IAG G+AS+ V NKWD VE +K F R+ F+ Sbjct: 267 VVIVLDATQGITRQDQRIAGLVERRGRASVVVFNKWDLVEHREKRYDEFTKLFREKLYFI 326 Query: 295 SYAPVVFLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPP 347 Y+P++F SA + +++ + + ++ + +N + +A P Sbjct: 327 DYSPLIFTSADKGWNIDRVIDAINLAYASYTTKVPSSAINSALQKVLAFTNLP 379 Score = 75.1 bits (183), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 52/195 (26%), Positives = 95/195 (48%), Gaps = 10/195 (5%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 V ++GRPNVGKS+L N ++ +++ IV D G TRD + E G+ + L+DT G+ Sbjct: 4 VLIVGRPNVGKSTLFNKLVKKKKAIVEDEEGVTRDPVQDTVEWYGKTFKLVDTCGVFDNP 63 Query: 212 KVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWD 271 + + + V M I AD+VL V++G+ GI +D+ +A + + +I V NK + Sbjct: 64 QDIISQKMKEVTLNM--IREADLVLFVVDGKRGITKEDESLADFLRKSTVDTILVANKAE 121 Query: 272 AVEKDDKTMQNFENTIRDHFLFMSYAPVVFLSALTKQRLQKLLPVVQHVAQQHALRITTH 331 ++ FE ++ + + + +SA L LL + ++ L + + Sbjct: 122 -------NLREFEREVKPELYSLGFGEPIPVSAEHNINLDTLLETIIKKLEEKGLDLESK 174 Query: 332 L-VNDVVSDAVAINP 345 + D + A+ P Sbjct: 175 PEITDAIKVAIVGRP 189 >CAC1651 [R] COG1160 Predicted GTPases Length = 411 Score = 65.1 bits (157), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 53/173 (30%), Positives = 84/173 (48%), Gaps = 32/173 (18%) Query: 150 IRVALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYV-LIDTAGM- 207 + +AL G+ NVGKSS++NA+ +E +VS+V GTT D + E V LIDTAG+ Sbjct: 13 LHIALFGKTNVGKSSVINALTSQEIALVSNVKGTTTDPVYKAMELLPLGPVMLIDTAGLD 72 Query: 208 -------RKRGKVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAG 260 +RGK E + + DV ++V + E GI + DK+I + Sbjct: 73 DISDLGELRRGKTLE------------VLSKTDVAILVFDVESGITEYDKNIYSLLLEKK 120 Query: 261 KASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVVFLSALTKQRLQKL 313 I V+NK D K D ++++ + + P+V +SAL + + L Sbjct: 121 IPLIGVLNKID---KKDYKLEDYTSQFK--------IPIVPISALNNKGINNL 162 >PH0344 [R] COG1100 GTPase SAR1 and related small G proteins Length = 186 Score = 58.5 bits (140), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 57/194 (29%), Positives = 99/194 (51%), Gaps = 31/194 (15%) Query: 149 IIRVALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFER------------DG 196 +I+VA+IG NVGKS+L+NA+LG + VS+ GTT+ I F + Sbjct: 1 MIKVAIIGAENVGKSTLMNALLGGKFSEVSETPGTTKGIIKRAFGKIKLPKTMKNPFGGA 60 Query: 197 QRYVLIDTAGM-----RKRGKVYETTEKYSVMRAMRAIERADVVLVVINGEEGI---IDQ 248 VLIDTAG+ RGKV + EK+ + ++ I ADV++ +I+ +G+ +++ Sbjct: 61 DELVLIDTAGLFDPEKEIRGKVL-SEEKFKEL--IKEIVNADVIIHMIDATKGLHRGMEK 117 Query: 249 DKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVVFLSALTKQ 308 ++ + ++ K I V+NK D V +++ R + P++ LS +T + Sbjct: 118 LHYMLKFRYE--KPIIVVINKIDLVSREELERLRELVKKR-----LEQEPIL-LSLVTYE 169 Query: 309 RLQKLLPVVQHVAQ 322 +LL + + AQ Sbjct: 170 GFNELLKALTYYAQ 183 >SMc00581 [O] COG0760 Parvulin-like peptidyl-prolyl isomerase Length = 316 Score = 56.6 bits (135), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 10/195 (5%), Positives = 30/195 (15%), Gaps = 25/195 (12%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 L G S+ + + I + ++ + Sbjct: 11 RLLSAVALAGALSVAAGTVTTAMAASTVEVVVNSTVITSGDVAKRVAFLKLQRQSG---- 66 Query: 212 KVYETTEKYSVMRAMR-----AIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFV 266 E + V GK Sbjct: 67 ---GAAEAKQQLVDEVLKRAEIARVQQSVSTDEVDAAFARF-AAGNKLSPEQLGKILDQA 122 Query: 267 VNKWDAVEKDDKTMQNFENTIRDHFLF--MSYAPVVFLSALTKQRLQKLLPVVQHVAQQH 324 D ++ ++ + + +R+ + Sbjct: 123 GVGVDHFKQYIAVQMSWP------RVVNFRYGSASRLSGGDLVKRMMQGGGNK----PVT 172 Query: 325 ALRITTHLVNDVVSD 339 ++ + Sbjct: 173 TEYFLQQVIFVIPES 187 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,092,435 Number of extensions: 1159738 Number of successful extensions: 32817 Number of sequences better than 1.0e-05: 9588 Number of HSP's gapped: 23246 Number of HSP's successfully gapped: 19168 Length of query: 375 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 267 Effective length of database: 25,922,359 Effective search space: 6921269853 Effective search space used: 6921269853 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)