BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0055.p (340 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0016_1 [RTKL] COG0515 Serine/threonine protein kinase 316 4e-86 MT3060 [C] COG0240 Glycerol-3-phosphate dehydrogenase 239 4e-63 Rv2982c [C] COG0240 Glycerol-3-phosphate dehydrogenase 238 1e-62 Rv0564c [C] COG0240 Glycerol-3-phosphate dehydrogenase 189 4e-48 MT0590 [C] COG0240 Glycerol-3-phosphate dehydrogenase 189 4e-48 >ML0016_1 [RTKL] COG0515 Serine/threonine protein kinase Length = 424 Score = 316 bits (809), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 19/334 (5%), Positives = 48/334 (14%), Gaps = 1/334 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 ++ H + + + A Sbjct: 8 SDRYELGDILGFGGMSEVHLARDIRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALN 67 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 ++ S+ +H + + + L Sbjct: 68 HPSIVAVYDTGEAETSAGPLPYIVMEYVDGATLRDIVHTDGPMPPQQAIEIVADACQALN 127 Query: 121 CS-EGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 S + I+ + V+ Sbjct: 128 FSHQNGIIHRDVKPANIMISATNAVKVMDFGIARAIADSTSVTQTAAVIGTAQYLSPEQA 187 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 G + V + G++ D Sbjct: 188 RGDSVDARSDVYSLGCVLYEILTGEPPFIGDSPVSVAYQHVREDPIPPSQRHEGISVDLD 247 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 VV + R + L V + T + + + Sbjct: 248 AVVLKALAKNPENRYQTAAEMRADLIRVHSGQPPEAPKVLTDADRSCLLSSGAGNFGVPR 307 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEMEAISQE 333 S + ++ Sbjct: 308 TDALSRQSLDETESDGSIGRWVAVVAVLAVLTIA 341 >MT3060 [C] COG0240 Glycerol-3-phosphate dehydrogenase Length = 334 Score = 239 bits (610), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 138/329 (41%), Positives = 186/329 (56%), Gaps = 7/329 (2%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 V+ AG+WGTALA VLA EV LW R+ E A +IN + N +LPG LPP+I AT D Sbjct: 10 VMGAGAWGTALAKVLADAGGEVTLWARRAEVADQINTTRYNPDYLPGALLPPSIHATADA 69 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 E A+ + V++ P+ MR P EG + KG E T+ RMS VI G Sbjct: 70 EEALGGASTVLLGVPAQTMRANLERWAPLLPEGATLVSLAKGIELGTLMRMSQVIISVTG 129 Query: 121 CSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGVE 180 ++ V+SGP+ A E+ P VV + FR YTN D VG E Sbjct: 130 AEPAQVAVISGPNLASEIAECQPAATVVACSDSGRAVALQRALNSGYFRPYTNADVVGTE 189 Query: 181 LAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGDL 240 + GA KNIIAL G++ G+G G+N AA++TRGLAEI R+G LGAN T +GLAG+GDL Sbjct: 190 IGGACKNIIALACGMAVGIGLGENTAAAIITRGLAEIIRLGTALGANGATLAGLAGVGDL 249 Query: 241 VVTATSKHSRNWRAGSMLGQGKPLAEVLESMGM---VVEGIRTTKAAYAISLNLNVQMPI 297 V T TS SRN G LG+G E L+S G VVEG+ + ++ A++ + +V+MP+ Sbjct: 250 VATCTSPRSRNRSFGERLGRG----ETLQSAGKACHVVEGVTSCESVLALASSYDVEMPL 305 Query: 298 TDQIYHVLFQGRTPRSAVEALMGRDRKTE 326 TD ++ V +G + A+ L+GR K E Sbjct: 306 TDAVHRVCHKGLSVDEAITLLLGRRTKPE 334 >Rv2982c [C] COG0240 Glycerol-3-phosphate dehydrogenase Length = 334 Score = 238 bits (607), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 138/329 (41%), Positives = 186/329 (56%), Gaps = 7/329 (2%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 V+ AG+WGTALA VLA EV LW R+ E A +IN + N +LPG LPP+I AT D Sbjct: 10 VMGAGAWGTALAKVLADAGGEVTLWARRAEVADQINTTRYNPDYLPGALLPPSIHATADA 69 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 E A+ + V++ P+ MR P EG + KG E T+ RMS VI G Sbjct: 70 EEALGGASTVLLGVPAQTMRANLERWAPLLPEGATLVSLAKGIELGTLMRMSQVIISVTG 129 Query: 121 CSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGVE 180 ++ V+SGP+ A E+ P VV + FR YTN D VG E Sbjct: 130 AEPPQVAVISGPNLASEIAECQPAATVVACSDSGRAVALQRALNSGYFRPYTNADVVGTE 189 Query: 181 LAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGDL 240 + GA KNIIAL G++ G+G G+N AA++TRGLAEI R+G LGAN T +GLAG+GDL Sbjct: 190 IGGACKNIIALACGMAVGIGLGENTAAAIITRGLAEIIRLGTALGANGATLAGLAGVGDL 249 Query: 241 VVTATSKHSRNWRAGSMLGQGKPLAEVLESMGM---VVEGIRTTKAAYAISLNLNVQMPI 297 V T TS SRN G LG+G E L+S G VVEG+ + ++ A++ + +V+MP+ Sbjct: 250 VATCTSPRSRNRSFGERLGRG----ETLQSAGKACHVVEGVTSCESVLALASSYDVEMPL 305 Query: 298 TDQIYHVLFQGRTPRSAVEALMGRDRKTE 326 TD ++ V +G + A+ L+GR K E Sbjct: 306 TDAVHRVCHKGLSVDEAITLLLGRRTKPE 334 >Rv0564c [C] COG0240 Glycerol-3-phosphate dehydrogenase Length = 341 Score = 189 bits (481), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 118/321 (36%), Positives = 163/321 (50%), Gaps = 6/321 (1%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPG-ITLPPNIIATTD 59 VL GSWGT +AS+ A P + W R A +IN++H N +L + L + ATTD Sbjct: 12 VLGGGSWGTTVASICARRGPTLQ-WVRSAVTAQDINDNHRNSRYLGNDVVLSDTLRATTD 70 Query: 60 METAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEEL 119 A + VV+ PS R V L + + + KG E T RMS +I E L Sbjct: 71 FTEAANCADVVVMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNMRMSQIIEEVL 130 Query: 120 -GCSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVG 178 G G +L+GP+ A EV V+ +F FRVYT D VG Sbjct: 131 PGHPAG---ILAGPNIAREVAEGYAAAAVLAMPDQHLATRLSAMFRTRRFRVYTTDDVVG 187 Query: 179 VELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLG 238 VE AGALKN+ A+ G+ LG G+N +A ++ R L E++++GV +G TF GLAGLG Sbjct: 188 VETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETFPGLAGLG 247 Query: 239 DLVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPIT 298 DL+VT TS+ SRN G LG GKP+ E++ SM V EG++ + + MPI Sbjct: 248 DLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEFGLNMPIA 307 Query: 299 DQIYHVLFQGRTPRSAVEALM 319 ++ V+ G T A L+ Sbjct: 308 REVDAVINHGSTVEQAYRGLI 328 >MT0590 [C] COG0240 Glycerol-3-phosphate dehydrogenase Length = 341 Score = 189 bits (481), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 118/321 (36%), Positives = 163/321 (50%), Gaps = 6/321 (1%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPG-ITLPPNIIATTD 59 VL GSWGT +AS+ A P + W R A +IN++H N +L + L + ATTD Sbjct: 12 VLGGGSWGTTVASICARRGPTLQ-WVRSAVTAQDINDNHRNSRYLGNDVVLSDTLRATTD 70 Query: 60 METAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEEL 119 A + VV+ PS R V L + + + KG E T RMS +I E L Sbjct: 71 FTEAANCADVVVMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNMRMSQIIEEVL 130 Query: 120 -GCSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVG 178 G G +L+GP+ A EV V+ +F FRVYT D VG Sbjct: 131 PGHPAG---ILAGPNIAREVAEGYAAAAVLAMPDQHLATRLSAMFRTRRFRVYTTDDVVG 187 Query: 179 VELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLG 238 VE AGALKN+ A+ G+ LG G+N +A ++ R L E++++GV +G TF GLAGLG Sbjct: 188 VETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETFPGLAGLG 247 Query: 239 DLVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPIT 298 DL+VT TS+ SRN G LG GKP+ E++ SM V EG++ + + MPI Sbjct: 248 DLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEFGLNMPIA 307 Query: 299 DQIYHVLFQGRTPRSAVEALM 319 ++ V+ G T A L+ Sbjct: 308 REVDAVINHGSTVEQAYRGLI 328 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,530,003 Number of extensions: 836474 Number of successful extensions: 2009 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 1990 Number of HSP's successfully gapped: 9 Length of query: 340 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 233 Effective length of database: 26,037,693 Effective search space: 6066782469 Effective search space used: 6066782469 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)