BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0043.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr5252 [H] COG2226 Methylase involved in ubiquinone/menaquinone... 162 6e-40 mlr3165 [H] COG2226 Methylase involved in ubiquinone/menaquinone... 151 9e-37 SMc01155 [H] COG2226 Methylase involved in ubiquinone/menaquinon... 140 2e-33 sll1653 [H] COG2226 Methylase involved in ubiquinone/menaquinone... 140 3e-33 NMA1319 [R] COG4228 Mu-like prophage DNA circulation protein 80 3e-15 >alr5252 [H] COG2226 Methylase involved in ubiquinone/menaquinone biosynthesis Length = 359 Score = 162 bits (409), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 11/224 (4%), Positives = 31/224 (13%), Gaps = 4/224 (1%) Query: 21 VHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCGTCDWSIALAE- 79 + L+ + + Sbjct: 5 TVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEAL 64 Query: 80 ASKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNSFDYATIGFGLR 139 + G + R ++ + + + T+ L Sbjct: 65 RLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLD 124 Query: 140 NVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQR-VLPLLGKLFAKRYE 198 + V E L Sbjct: 125 TGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTF--C 182 Query: 199 QYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHI 242 + K S+A + + + ++ Sbjct: 183 EGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTG 226 >mlr3165 [H] COG2226 Methylase involved in ubiquinone/menaquinone biosynthesis Length = 258 Score = 151 bits (381), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 88/257 (34%), Positives = 130/257 (50%), Gaps = 12/257 (4%) Query: 1 MTIEKTTTIGDQ---------GTKPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFT 51 M++E+TT G G K+ V+ VF +A +YDLMND++S H+ W+ Sbjct: 1 MSVERTTAAGGMETSYGFKRVGEGEKQSLVNDVFHKVANRYDLMNDLMSAGLHRLWKDAM 60 Query: 52 MRKMGMKR--GDSAVDLCCGTCDWSIALAEASKTGC-VMGLDFSAGMLEVGRRKVEARNL 108 + + + G +D+ GT D + + +AS LD + ML VGR + + + L Sbjct: 61 VTWLNPPKRPGWKVLDVAGGTGDIAFRIVDASHGNAHATVLDINGSMLGVGRDRAQKKGL 120 Query: 109 QDRISLVQGNAMELPFGDNSFDYATIGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSK 168 V+ NA ELPF D +FD TI FG+RNVP L+E RV+KPGG +CLE S+ Sbjct: 121 SGNTDFVEANAEELPFADATFDAYTIAFGIRNVPRIDVALSEAFRVLKPGGRFLCLEFSE 180 Query: 169 PMKQPFKGIYYFYFQRVLPLLGKLFAKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKV 228 Y + +P +GK+ E Y +L ES+A FP+++ + G +V Sbjct: 181 VEMPLLDKAYEAWSFNAIPKIGKMVTGDGEPYSYLVESIAKFPNQQNFAAMISRAGFDRV 240 Query: 229 ESFPLTGGIAALHIGLK 245 +GGIAALH G K Sbjct: 241 SFRNYSGGIAALHSGWK 257 >SMc01155 [H] COG2226 Methylase involved in ubiquinone/menaquinone biosynthesis Length = 270 Score = 140 bits (352), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 84/239 (35%), Positives = 124/239 (51%), Gaps = 9/239 (3%) Query: 13 GTKPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMK-RGDSAV-DLCCGT 70 GT K+ V+ VF +A +YD+MND++S H+ W+ + + R D V D+ GT Sbjct: 34 GTGEKQPLVNDVFHKVAKRYDIMNDVMSAGLHRVWKDAMIAALNPPGREDYRVLDVAGGT 93 Query: 71 CDWSIALAEAS-KTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNSF 129 D + + E S + LD + ML VG + + ++ + V+ NA +LPF NSF Sbjct: 94 GDIAFRIVERSGRKAHATVLDINGSMLSVGAERARKKGIEANLDFVEANAEDLPFAANSF 153 Query: 130 DYATIGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSK---PMKQPFKGIYYFYFQRVL 186 D TI FG+RNVP L E RV+K GG ++ LE S+ P+ F + F + Sbjct: 154 DAYTIAFGIRNVPRIEVALKEAYRVLKRGGRLLVLEFSEVEMPLLDRFYDAWSF---NAI 210 Query: 187 PLLGKLFAKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGLK 245 P GKL Y++L ES+ FP+++ + R+ G +V TGGIAALH G K Sbjct: 211 PKFGKLITGDDAPYQYLVESIRKFPNQRDFAAMIRDAGFARVSFTNYTGGIAALHSGWK 269 >sll1653 [H] COG2226 Methylase involved in ubiquinone/menaquinone biosynthesis Length = 238 Score = 140 bits (352), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 77/233 (33%), Positives = 125/233 (53%), Gaps = 2/233 (0%) Query: 14 TKPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCGTCDW 73 T+P ++ V +F IA +YD +N LSF +H W+ ++ G+ GD +D+CCG+ D Sbjct: 7 TQPTQESVQQIFARIAPQYDDLNTFLSFGQHHIWKAMAVKWSGVSPGDRLLDVCCGSGDL 66 Query: 74 SIALAEASKT-GCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNSFDYA 132 + A+ T G V+GLDF A +L + K +++ + +QG+A+ LPF DN FD A Sbjct: 67 AFQGAKVVGTRGKVVGLDFCAELLAIAAGKHKSKYAHLPMQWLQGDALALPFSDNEFDGA 126 Query: 133 TIGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQRVLPLLGKL 192 T+G+GLRNV + + L E++RV+KPG V L+ +P +Y V+ + K Sbjct: 127 TMGYGLRNVGNIPQALTELQRVLKPGKKVAILDFHQPGNALAANFQRWYLANVVVPMAKQ 186 Query: 193 FAKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGLK 245 + + E+Y +L SL FP + E G K +P+ G+ + + K Sbjct: 187 W-RLTEEYAYLQPSLDRFPTGPKQVQFALEVGFAKAVHYPIAAGLMGVLVAEK 238 >NMA1319 [R] COG4228 Mu-like prophage DNA circulation protein Length = 199 Score = 80.1 bits (196), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 50/145 (34%), Positives = 75/145 (51%), Gaps = 10/145 (6%) Query: 21 VHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCGTCDWSIALAEA 80 V S + IA YDLM + F + WR+ T+ + + +++ GT L Sbjct: 3 VISKYNRIAAIYDLMELPMEFFLYGRWREETLSDLQGR----VLEVGVGTGR---NLKYY 55 Query: 81 SKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNSFDYATIGFGLRN 140 S V G+D S GMLE R+K E ++L+ +A L F D +FDY F L + Sbjct: 56 SSGSSVTGIDVSEGMLEKARKKAEG---MKNVNLLLMDAEHLEFSDKTFDYVITTFVLCS 112 Query: 141 VPDPVRVLNEMKRVVKPGGMVVCLE 165 +PDPV+ L EM+RV+KP G ++ +E Sbjct: 113 IPDPVKALKEMRRVLKPSGELIAIE 137 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,291,260 Number of extensions: 678758 Number of successful extensions: 1242 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 1265 Number of HSP's successfully gapped: 40 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 114 (48.5 bits)