BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0042.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0851 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and r... 183 2e-46 AF2176 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and r... 157 2e-38 Ta1198 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and r... 122 8e-28 TVN0407 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and ... 115 6e-26 PAE2375 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and ... 94 2e-19 >DR0851 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 313 Score = 183 bits (465), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 114/283 (40%), Positives = 156/283 (55%), Gaps = 1/283 (0%) Query: 4 KIGIFLQMIKFEHTVFALPFAFMGALLGSVVMFG-ALPSWGQIGWIVVAMFGARSAAMGL 62 ++ +L+++KFEHTVFALPFA+ G LL S+ G P W + W+ VAM AR+AAM Sbjct: 25 QVKTYLELVKFEHTVFALPFAYAGMLLASMAQNGTGWPGWHTLIWVTVAMAAARTAAMAA 84 Query: 63 NRLIDRFSDAKNPRTAGRAIPAGLLKVGEVSXXXXXXXXXXXXXXXKLNPLSAKLLPIAV 122 NR+IDR DA+NPRTAGR +P+G + + +LNPL LLP+A+ Sbjct: 85 NRVIDRAIDARNPRTAGREVPSGKVSPAQAWGLVAVSVAVLLFAAWQLNPLCVALLPLAL 144 Query: 123 FLLVFYSFTKRFTWACHLILGLTIALAPLXXXXXXXXXXXXXSMVFYFTIVFWTAGFDII 182 L+ Y +TKRFTW CH LG+T A + + T+VFW G D I Sbjct: 145 LFLIGYPYTKRFTWLCHAWLGVTDGAAAAGGWIAVTGHFSPVAWALWATVVFWMIGLDTI 204 Query: 183 YSCQDVEFDKKEGLYSIPVRFGVARALTIAKVFHMLTGIGFVSLLFITDLSWWYVAGMLI 242 Y+ D FD+ G+ SIP RFG+ AL IA V H+LT + + + S WY L+ Sbjct: 205 YATMDYAFDRANGVKSIPARFGIPAALNIAAVSHVLTFGLLLLVGVLAGASVWYYLAALV 264 Query: 243 AYIILFYEHHIVSPSDLSRLQTAFFTMNGVLSIVVFSFTLIDL 285 IL +EH IV P DL R+ AFF N L+I + ++D+ Sbjct: 265 MGAILLFEHRIVDPQDLGRVNVAFFDANMWLAITMLLGVVMDV 307 >AF2176 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 291 Score = 157 bits (396), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 99/279 (35%), Positives = 145/279 (51%), Gaps = 11/279 (3%) Query: 2 LKKIGIFLQMIKFEHTVFALPFAFMGALLGSVVMFGALPSWGQIGWIVVAMFGARSAAMG 61 +++I ++L IK EHT+FALPFA+ GA L G L S I+ A G R+AAM Sbjct: 1 MQRIKMYLDFIKIEHTLFALPFAYAGAFLAE----GGLISLRLAFLILTAFTGLRTAAMT 56 Query: 62 LNRLIDRFSDAKNPRTAGRAIPAGLLKVGEVSXXXXXXXXXXXXXXXKLNPLSAKLLPIA 121 NR+IDR DAKNPRTA R +PAGL+ + E +N + L PI Sbjct: 57 FNRIIDREIDAKNPRTAMRHLPAGLISLKEAYTIAFLALAIYFISAALINKTALMLSPIP 116 Query: 122 VFLLVFYSFTKRFTWACHLILGLTIALAPL-----XXXXXXXXXXXXXSMVFYFTIVFWT 176 Y + KRFT CH +LGL +A APL + ++FW Sbjct: 117 AITAYIYPYLKRFTCLCHYVLGLNLAFAPLGGWIAVTDSIDIFGSELVPTLIGVAVMFWV 176 Query: 177 AGFDIIYSCQDVEFDKKEGLYSIPVRFGVARALTIAKVFHMLTGIGFVSLLFITDLSWWY 236 AGFD+IY QDV+FD+ L+SI FGV AL ++++ H++ +++ + Sbjct: 177 AGFDMIYGLQDVDFDRSNNLHSIGAHFGVRAALWLSRLNHVIF-FALIAIALQIHNAKLA 235 Query: 237 VAGMLIAYIILFYEHHIVSPS-DLSRLQTAFFTMNGVLS 274 + + ++LFYEH+IV D +++Q AFF +N ++S Sbjct: 236 ELFLPLIALLLFYEHYIVRDGYDEAKIQIAFFYVNALVS 274 >Ta1198 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 286 Score = 122 bits (305), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 82/262 (31%), Positives = 128/262 (48%), Gaps = 7/262 (2%) Query: 12 IKFEHTVFALPFAFMGALLGSVVMFGALPSWGQIGWIVVAMFGARSAAMGLNRLIDRFSD 71 IK EHTVF LPF F G +L + G +I I++A AR++AM +NR+ D Sbjct: 10 IKLEHTVFDLPFIFTGYVLAA----GRYIYPIKILLILIAAVSARASAMSINRIEGLRYD 65 Query: 72 AKNPRTAGRAIPAGLLKVGEVSXXXXXXXXXXXXXXXKLNPLSAKLLPIAVFLLVFYSFT 131 NPR A+ +G + E LN L L P+ +FL + Sbjct: 66 VINPRKKDWALVSGRISKREAIAMTIFFIALFEMATYFLNRLVFILSPVVIFLFLTDPLL 125 Query: 132 KRFTWACHLILGLTIALAPLXXXXXXXXXXXXXSMVFYFTI--VFWTAGFDIIYSCQDVE 189 KR T H+ +G TI + L ++ I FW AGFD+IY D+E Sbjct: 126 KRITPWRHVYMGSTIGVGVLAGYLAVIPAFPRELTIYLIFIGSSFWIAGFDVIYVIPDIE 185 Query: 190 FDKKEGLYSIPVRFGVARALTIAKVFHMLTGIGFVSLLFITDLSWWYVAGMLIAYIILFY 249 +D+ GL ++ VR+G+ R L I+ +FH +T I F +++ ++WY+A M++ ++ Y Sbjct: 186 YDRINGLKTLMVRYGLKRGLQISIIFHAVTLISFWAVMLYVR-TYWYLAAMIVISFLVVY 244 Query: 250 EHHIVSPSDLSRLQTAFFTMNG 271 +H I+ PS ++ +FF N Sbjct: 245 QHVILDPSKPETVRRSFFNANS 266 >TVN0407 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 286 Score = 115 bits (289), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 83/265 (31%), Positives = 126/265 (47%), Gaps = 7/265 (2%) Query: 9 LQMIKFEHTVFALPFAFMGALLGSVVMFGALPSWGQIGWIVVAMFGARSAAMGLNRLIDR 68 + IK EHTVF LPF L G V+ G +I I++A ARS+AM +NR+ Sbjct: 7 VDYIKLEHTVFDLPFI----LSGYVIAAGTSIFPYKIILILIAAVTARSSAMSINRIEGL 62 Query: 69 FSDAKNPRTAGRAIPAGLLKVGEVSXXXXXXXXXXXXXXXKLNPLSAKLLPIAVFLLVFY 128 D NPR A+ +G + V E LN L L PI +FL V Sbjct: 63 RYDLTNPRKKEWALVSGRMTVREAIALTILFIAVFEISAFLLNRLVFILSPIVIFLFVSD 122 Query: 129 SFTKRFTWACHLILGLTIALAPLXXXXXXXXXXXXXSMVFYFTIV--FWTAGFDIIYSCQ 186 KR+T H +G I + L +++ I W AGFD+IY Sbjct: 123 PILKRYTEWRHFYMGSAIGIGTLAGYLAVIPEFPSNLAIYFLFIASSLWIAGFDMIYVIP 182 Query: 187 DVEFDKKEGLYSIPVRFGVARALTIAKVFHMLTGIGFVSLLFITDLSWWYVAGMLIAYII 246 D+E+D+K L ++ ++G+ L I+ + H+ I F +L+ + WY+AG++I I+ Sbjct: 183 DIEYDQKNKLKTVMTKYGIKLGLLISDITHLAALIFFWLMLYYFR-TIWYLAGLIIITIL 241 Query: 247 LFYEHHIVSPSDLSRLQTAFFTMNG 271 + Y+H IV P+D ++ +FF N Sbjct: 242 VIYQHIIVDPADPLSIRKSFFNANS 266 >PAE2375 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 291 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 67/204 (32%), Positives = 90/204 (44%), Gaps = 10/204 (4%) Query: 10 QMIKFEHTVFALPFAFMGALLGSVVMFGALPSWGQIGWIVVAMFGARSAAMGLNRLIDRF 69 + I+ EHT+F LP A+ ALL G L W I +I +A+FG R+A M N + D Sbjct: 17 RFIRIEHTIFTLPMAYAAALLTG----GELDLWKAI-FIGLAVFGLRTAGMSWNNIADYP 71 Query: 70 SDAKNPRTAGRAIPAGLLKVGEVSXXXXXXXXXXXXXXXKLNPLSAKLLPIAVFLLVFYS 129 D NPRT GR + AG + E L P L + +++ Y Sbjct: 72 LDKLNPRTQGRMLVAGKMSFKEAYGVFFTGITIFLISAAMLGPWPLALSIPYLIVVITYP 131 Query: 130 FTKRFTWACHLILGLTIALAPLXXXXXXXXXXXXXSMV-----FYFTIVFWTAGFDIIYS 184 + KR HL LG AL PL ++ F W AGFD+IYS Sbjct: 132 YAKRLHCLPHLHLGAVYALVPLGAAIAMHPLDINAALAKTPWFLVFAAALWVAGFDVIYS 191 Query: 185 CQDVEFDKKEGLYSIPVRFGVARA 208 D +FDK +GL S+ FG+ A Sbjct: 192 KGDYDFDKSQGLGSVAACFGLKAA 215 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.333 0.145 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,051,708 Number of extensions: 679316 Number of successful extensions: 2204 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 2179 Number of HSP's successfully gapped: 11 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 115 (48.9 bits)