BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0037.p (185 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2221 [NT] COG1352 Methylase of chemotaxis methyl-accepting pr... 111 6e-25 MA0013 [NT] COG1352 Methylase of chemotaxis methyl-accepting pro... 102 5e-22 MA3063 [NT] COG1352 Methylase of chemotaxis methyl-accepting pro... 93 3e-19 BB0040 [NT] COG1352 Methylase of chemotaxis methyl-accepting pro... 91 9e-19 VCA1091 [NT] COG1352 Methylase of chemotaxis methyl-accepting pr... 90 2e-18 >CAC2221 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 255 Score = 111 bits (277), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 62/145 (42%), Positives = 85/145 (58%) Query: 22 VKQSTGIDLAQYKEAQMKRRLTTLRMKNGYQSFSDFFAAMMKDKALFYEFLDRMTINVSE 81 V + G+DL YKE Q+ RR+ +L + G S ++ + + KD FLD +TINV+E Sbjct: 10 VLKDFGLDLFAYKENQLHRRILSLISRVGASSIDEYVSMLKKDPEQRQRFLDFITINVTE 69 Query: 82 FWRNPNRWEVLRDVILPELQRSGRRLKLWSAACSTGEEPYTLAMILSDKNILAQTGILAT 141 F+RNP + I L S LK+WSAACS G EPY++A+IL + L + I+AT Sbjct: 70 FFRNPEIFREAEKEIKNMLYSSKEPLKIWSAACSIGAEPYSIAIILDKLDSLRKNNIIAT 129 Query: 142 DIDDGALAKAKQGIYLERSLKDVPK 166 DID L KAK G Y E +K+V K Sbjct: 130 DIDSTILNKAKLGEYTENEIKNVEK 154 >MA0013 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 382 Score = 102 bits (253), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 58/165 (35%), Positives = 91/165 (55%), Gaps = 13/165 (7%) Query: 11 PDPDYTGFIHNVKQSTGIDLAQYKEAQMKRRLTTLRMKNGYQSFSDFFAAMMKDKALFYE 70 DP + V +STG + YKE +RR+ +++ ++ + KD A + Sbjct: 110 EDPGFELLKRVVTESTGFNCEHYKETHFRRRINIRIRATNSENYEEYLRVLKKDSAEYEH 169 Query: 71 FLDRMTINVSEFWRNPNRWEVLRDVILPELQRSG-----RRLKLWSAACSTGEEPYTLAM 125 + +TINVSEF+RNP + V+ ++P L +S + +++WSA C+TGEE Y+LA+ Sbjct: 170 LVKALTINVSEFFRNPETFGVIEKEVIPYLIKSRSDSLVKSIRIWSAGCATGEEAYSLAI 229 Query: 126 ILSDKNILA------QTGILATDIDDGALAKAKQGIYLERSLKDV 164 +L IL + I+ TDIDD +L KA +GIY E LK+V Sbjct: 230 LLH--RILGRDFGRYRISIIGTDIDDLSLKKALKGIYRENVLKNV 272 >MA3063 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 270 Score = 92.8 bits (229), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 53/162 (32%), Positives = 92/162 (56%), Gaps = 12/162 (7%) Query: 12 DPDYTGFIHNVKQSTGIDLAQYKEAQMKRRLTTLRMKN-GYQSFSDFFAAMMKDKALFYE 70 D D+ I + +S+GI L Y+++ ++RR+ LRMK G +++ + + +++ E Sbjct: 6 DEDFKFLIRKISKSSGIVLTGYRDSYLRRRID-LRMKAVGADNYATYKGLLERNQNEMKE 64 Query: 71 FLDRMTINVSEFWRNPNRWEVLRDVILPELQRSGRR-----LKLWSAACSTGEEPYTLAM 125 ++ +T+NV+EF R+ + R ILPE+ + ++ WSAACS GEEPY++ Sbjct: 65 VINALTVNVTEFMRDKTPFLFFRKEILPEIMERKNKSRSNIVRFWSAACSYGEEPYSIG- 123 Query: 126 ILSDKNILAQ---TGILATDIDDGALAKAKQGIYLERSLKDV 164 + + +L + I ATDID+ L A QGIY + LK++ Sbjct: 124 -ICSREVLPEDWSVSIYATDIDEKCLKDASQGIYRKEQLKNL 164 >BB0040 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 283 Score = 90.9 bits (224), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 49/156 (31%), Positives = 90/156 (57%), Gaps = 11/156 (7%) Query: 22 VKQSTGIDLAQYKEAQMKRRLTTLRMKNGYQSFSDFFAAMMKD--KALFYEFLDRMTINV 79 V + GI+L++ K+ ++ RL++L G+++F+++ + K E +D+++ N Sbjct: 26 VYNNFGINLSEKKKLLIESRLSSLLKVKGFKNFTEYINFLEKSTGNIQLIELIDKISTNH 85 Query: 80 SEFWRNPNRWEVLRDVILPEL-----QRSGRRLKLWSAACSTGEEPYTLAMILSD----K 130 + F+R ++ L + ILP+L +++WSA CS+GEEPYT+AM+L + Sbjct: 86 TYFFRESKHFDFLNNKILPKLTEKILNSENSEIRIWSAGCSSGEEPYTIAMMLKEYMEHN 145 Query: 131 NILAQTGILATDIDDGALAKAKQGIYLERSLKDVPK 166 + + ILATDI L +A++GIY E + ++PK Sbjct: 146 RVNFKVKILATDISISVLNEAREGIYPEDRIINLPK 181 >VCA1091 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 276 Score = 89.7 bits (221), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 60/189 (31%), Positives = 90/189 (47%), Gaps = 8/189 (4%) Query: 1 MAEREEAATAPDPDYTGFIHNVKQSTGIDLAQYKEAQMKRRLTTLRMKNGYQSFSDFFAA 60 +A E+ D D+ + ++ GI L K + RL+ + G + F+ F Sbjct: 2 LAVNEQEFELTDKDFKFIQWFMHKTVGIYLPDSKRTMVYGRLSRQMRRKGLRRFTQFREL 61 Query: 61 MMKDKALFYEFLDRMTINVSEFWRNPNRWEVLRDVILPEL--QRSGRRLKLWSAACSTGE 118 + D+ F++ +T N +EF+R + +E + V++PE +R G +L+ WSA CSTGE Sbjct: 62 IESDEQERIHFINTLTTNKTEFFRESHHFEFIEKVLVPEWSKERVG-QLRFWSAGCSTGE 120 Query: 119 EPYTLAMILSDK---NILAQTGILATDIDDGALAKAKQGIYLERSLKDVPKXXXXRRPLF 175 EPYTL +L N I ATD+D L KA GIY S +P+ R F Sbjct: 121 EPYTLVSVLDHAGVMNFCPDIKIWATDLDTAVLEKASLGIYPIESQSSIPERYLRR--CF 178 Query: 176 YTGRAGVQG 184 G QG Sbjct: 179 VRGVKDQQG 187 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,111,442 Number of extensions: 475394 Number of successful extensions: 781 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 744 Number of HSP's successfully gapped: 24 Length of query: 185 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 85 Effective length of database: 26,845,031 Effective search space: 2281827635 Effective search space used: 2281827635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 112 (47.8 bits)