BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0036.p (89 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2221 [NT] COG1352 Methylase of chemotaxis methyl-accepting pr... 103 5e-23 VC2201 [NT] COG1352 Methylase of chemotaxis methyl-accepting pro... 69 2e-12 BB0040 [NT] COG1352 Methylase of chemotaxis methyl-accepting pro... 68 4e-12 VCA1091 [NT] COG1352 Methylase of chemotaxis methyl-accepting pr... 64 5e-11 PA3348 [NT] COG1352 Methylase of chemotaxis methyl-accepting pro... 62 2e-10 >CAC2221 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 255 Score = 103 bits (258), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 46/85 (54%), Positives = 62/85 (72%) Query: 4 VSEGLKKNIDFRKQNLLLDKFDEGFDLIICRNVMIYFTEEAKNKLYHKFSASLRPGGYLF 63 + + +KK I F+K +L+LD +D FDL+ICRNV+IYF ++ K K+Y KFS SL+ GG LF Sbjct: 170 IVDKIKKMITFKKHDLILDSYDNNFDLVICRNVVIYFNQDVKEKIYKKFSQSLKKGGLLF 229 Query: 64 VGSTEQIFTPAQYGFESTETFFYRK 88 VG+TE I+ +YGFE TF YRK Sbjct: 230 VGATESIYNYKEYGFEKASTFIYRK 254 >VC2201 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 275 Score = 68.6 bits (166), Expect = 2e-12, Method: Composition-based stats. Identities = 31/69 (44%), Positives = 50/69 (72%), Gaps = 1/69 (1%) Query: 3 KVSEGLKKNIDFRKQNLLLDKFDEG-FDLIICRNVMIYFTEEAKNKLYHKFSASLRPGGY 61 KV + +K+ ++FR QNL+ G FD+I CRNV+IYF+ + K+K+ ++ +ASL PGGY Sbjct: 186 KVKDNVKRLVNFRPQNLMESYSLLGKFDIIFCRNVLIYFSPDMKSKVLNQMAASLNPGGY 245 Query: 62 LFVGSTEQI 70 L +G++E + Sbjct: 246 LLLGASESL 254 >BB0040 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 283 Score = 67.8 bits (164), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 38/85 (44%), Positives = 52/85 (61%), Gaps = 10/85 (11%) Query: 2 FKVSEGLKKNIDFRKQNLLLDKF--DEGFDLIICRNVMIYFTEEAKNKLYHKFSASLRPG 59 F+V E LKK + F+K NL+ +KF + FDLI CRNVMIYF E+ +N L +KF++ L+ Sbjct: 196 FQVKEILKKMVYFKKLNLMDEKFPFSKKFDLIFCRNVMIYFDEKTRNNLANKFNSYLKKD 255 Query: 60 GYLFVGSTEQI--------FTPAQY 76 YL +G +E I PA Y Sbjct: 256 SYLLIGHSETIRGNKNLEYIMPATY 280 >VCA1091 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 276 Score = 64.3 bits (155), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 28/70 (40%), Positives = 44/70 (62%), Gaps = 1/70 (1%) Query: 2 FKVSEGLKKNIDFRKQNLLLD-KFDEGFDLIICRNVMIYFTEEAKNKLYHKFSASLRPGG 60 K+ + L++ IDF + NL+ + F + DLI+CRNVMIYF + +L +F L+PGG Sbjct: 189 MKIKQSLQRYIDFHQLNLIQEWPFKQKLDLILCRNVMIYFDRPTQEQLIERFHQQLKPGG 248 Query: 61 YLFVGSTEQI 70 L +G +E + Sbjct: 249 VLMLGHSESV 258 >PA3348 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 274 Score = 61.6 bits (148), Expect = 2e-10, Method: Composition-based stats. Identities = 32/91 (35%), Positives = 51/91 (56%), Gaps = 3/91 (3%) Query: 2 FKVSEGLKKNIDFRKQNLLLDKFDEG-FDLIICRNVMIYFTEEAKNKLYHKFSASLRPGG 60 + V ++ ++FR NLL G FD++ CRNV+IYF+ E K + + +L+PGG Sbjct: 184 WAVKPAIRSRVEFRALNLLDSYASLGKFDMVFCRNVLIYFSAEVKRDILLRIHGTLKPGG 243 Query: 61 YLFVGSTEQIFT-PAQYGF-ESTETFFYRKK 89 YLF+G++E + P Y + + YR K Sbjct: 244 YLFLGASEALNNLPDHYQMVQCSPGIIYRAK 274 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.142 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 6,221,120 Number of extensions: 209911 Number of successful extensions: 498 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 494 Number of HSP's successfully gapped: 19 Length of query: 89 Length of database: 38,378,431 Length adjustment: 59 Effective length of query: 30 Effective length of database: 31,573,725 Effective search space: 947211750 Effective search space used: 947211750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 109 (46.6 bits)