BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0031.p (509 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all0328 [EH] COG0147 Anthranilate/para-aminobenzoate synthases c... 397 e-110 aq_582 [EH] COG0147 Anthranilate/para-aminobenzoate synthases co... 395 e-110 BH1659 [EH] COG0147 Anthranilate/para-aminobenzoate synthases co... 393 e-109 XF0210 [EH] COG0147 Anthranilate/para-aminobenzoate synthases co... 366 e-101 PA0609 [EH] COG0147 Anthranilate/para-aminobenzoate synthases co... 364 e-100 >all0328 [EH] COG0147 Anthranilate/para-aminobenzoate synthases component I Length = 504 Score = 397 bits (1021), Expect = e-110, Method: Compositional matrix adjust. Identities = 213/492 (43%), Positives = 297/492 (60%), Gaps = 14/492 (2%) Query: 4 LSREYNLIPVVTRLLADMETPIRLFQRF-AGQDRAFLLESVEGGIQWARYSFIGSDPFLM 62 L+ + N +PV +AD++TP+ + + AG+ +FLLESVEGG + RYS +G DP + Sbjct: 12 LALQGNFVPVYQEWVADLDTPVSAWYKVCAGEPYSFLLESVEGGEKIGRYSLLGCDPLWV 71 Query: 63 VSXXXXXXXXXXXXXXRRL-SGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQ 121 + + + +G P L L Y+ KL +PP GG GF+GY+L++ Sbjct: 72 LEARGETTTQTNRDGSQVVFTGDPFTALADCLAPYKPVKLPQLPPGIGGLFGFWGYELIR 131 Query: 122 YYE-KLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEE 180 + E ++ H D+ N+ D +M D +++FD VK++I + ++D + D ++A Y++ Sbjct: 132 WIEPRVPVHPQDERNIPDGLWMQVDHLLIFDQVKRKIWAIAYADLRDPNVD--LKAAYQQ 189 Query: 181 LSRKLENMAEELQKE-GPKENVNRRSIPQDIELGEIH---SNLTREQYISNVEQAKEYIR 236 ++ M +L P++ + + P ++GEI SN TR ++ ++V++AKEYI+ Sbjct: 190 ACDRVTQMVSKLSLPLSPQKTILEWTPPGSQKVGEIQEYTSNFTRPEFCASVQKAKEYIK 249 Query: 237 AGDIFQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKV-- 294 AGDIFQVV+SQRL + P +YR LR +NPSPYM Y + I+G+SPE +VK Sbjct: 250 AGDIFQVVISQRLSTQYTGDPFSLYRSLRQINPSPYMAYFNFQDWQIIGSSPEVMVKAEL 309 Query: 295 --DGGRVET-RPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKF 351 DG + T RPIAGTRPRG+ H+MLVDLGRNDLGRV Sbjct: 310 DADGEAIATVRPIAGTRPRGKTTKEDAELAADLLQDPKEIAEHVMLVDLGRNDLGRVCAS 369 Query: 352 GTVKCDSFMEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIA 411 GTVK D M +ERYSHVMHIVSNV G L K+ +D +C PAGTVSGAPK+RAMEII Sbjct: 370 GTVKVDELMVVERYSHVMHIVSNVVGKLAANKNAWDLLKACFPAGTVSGAPKIRAMEIIN 429 Query: 412 ELEREARGAYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEET 471 ELE RG Y+G GY F G ++S I IRT++ R VQAGAG+V DS PEKEYEET Sbjct: 430 ELEPSRRGVYSGVYGYYDFEGQLNSAIAIRTMVVRDQTVTVQAGAGLVADSEPEKEYEET 489 Query: 472 VNKAKAMLKAIR 483 +NKA+ +L AIR Sbjct: 490 LNKARGLLLAIR 501 >aq_582 [EH] COG0147 Anthranilate/para-aminobenzoate synthases component I Length = 494 Score = 395 bits (1016), Expect = e-110, Method: Compositional matrix adjust. Identities = 210/488 (43%), Positives = 299/488 (61%), Gaps = 10/488 (2%) Query: 1 VLSLSREYNLIPVVTRLLADMETPIRLFQRFAGQDR-AFLLESVEGGIQWARYSFI-GSD 58 V LS YN+IP+ T LL D ETP+ +F + + + LLES EGG +W RYSFI Sbjct: 10 VRKLSENYNVIPLYTELLVDTETPLSIFLKLKEKGQFNILLESAEGGEKWGRYSFIITGS 69 Query: 59 PFLMVSXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYD 118 F + + + P+ ++K +++ + + +P F GG +G+F YD Sbjct: 70 SFYLRTRKDIGEIYERGKVNFFETKDPLSKIKEVVKKFIPYHDERLPRFWGGLVGYFAYD 129 Query: 119 LLQYYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGY 178 ++++YE + D ++ DI + D +++ D++ +I +V + ++G I Y Sbjct: 130 VVKFYEPVEDKNPDPIHTYDIYLVLTDVVVIHDNLTGKIKVVVPIFAQNG-----IEEEY 184 Query: 179 EELSRKLENMAEELQKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAG 238 E + + ++L++ G + ++ + SN T+E++ V++AKEYI G Sbjct: 185 ERAKNLIRDTVKKLKERG---TTFLNVVEKEPDFKNWRSNFTKEEFEDIVKKAKEYIAQG 241 Query: 239 DIFQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGR 298 D+ QVVLSQR + +P ++YR+LR LNPSPYMYYL D+ ++G+SPE LV+++ GR Sbjct: 242 DVIQVVLSQRFRKRFKGNPDNIYRVLRFLNPSPYMYYLDFDQLKVIGSSPEILVRLEEGR 301 Query: 299 VETRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDS 358 +ETRPIAGTR RG HLMLVDL RND+GRV+K G+V+ ++ Sbjct: 302 IETRPIAGTRKRGRTEEEDKRLEEDLLSDEKERAEHLMLVDLARNDIGRVAKTGSVRVEN 361 Query: 359 FMEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREAR 418 FM IERYSHVMHIVS+V G L EG D D + PAGTVSGAPK+RAM+II ELE E R Sbjct: 362 FMRIERYSHVMHIVSDVVGELREGYDALDVLKATFPAGTVSGAPKVRAMQIIEELENERR 421 Query: 419 GAYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAM 478 G YAG++GY+ F GNMD I IRT ++R +VQAGAGIV DSVPEKE+EETVNKAKA+ Sbjct: 422 GIYAGSVGYISFQGNMDMAIAIRTAVYRDRDIFVQAGAGIVADSVPEKEWEETVNKAKAL 481 Query: 479 LKAIRMAE 486 +KAI +AE Sbjct: 482 MKAIEIAE 489 >BH1659 [EH] COG0147 Anthranilate/para-aminobenzoate synthases component I Length = 502 Score = 393 bits (1009), Expect = e-109, Method: Compositional matrix adjust. Identities = 220/495 (44%), Positives = 301/495 (60%), Gaps = 16/495 (3%) Query: 1 VLSLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPF 60 V + ++++ IPV+ AD TPI + Q+ G + AF+LES + WAR+SFIG +PF Sbjct: 9 VKAANQQFRTIPVIGECFADAVTPIHIVQQL-GDEVAFILESKDDESPWARFSFIGLNPF 67 Query: 61 LMVSXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMP-----PFTGGAIGFF 115 + + + E+ K+ L+ LD P PF GGA+G+ Sbjct: 68 VELIEQNGVYQSISRSGSILVE---TEDFKSCLQQTID-VLDVQPLERELPFYGGAVGYM 123 Query: 116 GYDLLQYYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIR 175 YD ++ EK A+A + F+FC+ +IV+DH ++++ ++ L +DG + D R Sbjct: 124 AYDAIETIEKTLANARES-EAPLYHFLFCETLIVYDHTEKKLAVI-YLSTEDGGS-LDTR 180 Query: 176 AGYEELSRKLENMAEELQKEGPKENVNRRSIPQD-IELGEIHSNLTREQYISNVEQAKEY 234 Y+ +E + E + K + + R + QD + E+ SN +E ++ +VE+ KEY Sbjct: 181 --YKCAVNTIETVVETIHKRRDGDRLLRPAPVQDEVSFQEVSSNYEKEVFLRDVERIKEY 238 Query: 235 IRAGDIFQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKV 294 IRAGD+FQ VLSQR E V L VYR+LR +NPSPY++Y+K + +VG+SPE LV+V Sbjct: 239 IRAGDVFQAVLSQRFERELSVRALDVYRVLRMINPSPYLFYIKFHDVEVVGSSPERLVQV 298 Query: 295 DGGRVETRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTV 354 G VE PIAGTR RG H MLVDL RND+GR++++GTV Sbjct: 299 QDGHVEIHPIAGTRKRGATREEDEALAKELLADEKERAEHYMLVDLARNDIGRIAEYGTV 358 Query: 355 KCDSFMEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELE 414 K + +EI ++SHVMHI+S V+G L + DA PAGTVSGAPK+RAMEI+ ELE Sbjct: 359 KTPTLLEIGKFSHVMHIISKVTGELKQTLHPLDALRYGFPAGTVSGAPKIRAMEILNELE 418 Query: 415 REARGAYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNK 474 RG YAGAI YLGF GN+DSCI IRT+I + GRAY+QAGAGIV DSVPE EYEET NK Sbjct: 419 PTKRGIYAGAIAYLGFDGNIDSCIAIRTMIVKDGRAYIQAGAGIVADSVPENEYEETRNK 478 Query: 475 AKAMLKAIRMAEAMF 489 AKA+LKA+ MAEAMF Sbjct: 479 AKALLKAVEMAEAMF 493 >XF0210 [EH] COG0147 Anthranilate/para-aminobenzoate synthases component I Length = 487 Score = 366 bits (940), Expect = e-101, Method: Compositional matrix adjust. Identities = 199/479 (41%), Positives = 283/479 (59%), Gaps = 13/479 (2%) Query: 11 IPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIG--SDPFLMVSXXXX 68 IPVV + +D++TP+ ++ +FA +L ESVEGG +ARYS IG + Sbjct: 14 IPVVREVQSDLDTPLSVYLKFADAPYTYLFESVEGGEHFARYSIIGLPARRVYTFHGHTL 73 Query: 69 XXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQYYEK--L 126 R P+ E++ L Y P+L+G+P FTGG +G+FG++ +QY E + Sbjct: 74 VVSEFGKVLETRQVADPLAEVELLRAQYVVPQLEGLPGFTGGLVGWFGFECIQYIEPRFM 133 Query: 127 SAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEELSRKLE 186 D+L DI M + + VFD +K ++ L+ +H + RA +L+ Sbjct: 134 EGKKRDELGTPDILLMLSEELAVFDSLKGRLYLI--VHADPAQPHAYARA-----VERLD 186 Query: 187 NMAEELQKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDIFQVVLS 246 + L++ G + I + I + HS+LTREQY + V QA+EY+R+GDIFQVV S Sbjct: 187 VLIYRLRQGG--VDYPHSHISEVINEEDFHSSLTREQYHAVVRQAQEYVRSGDIFQVVPS 244 Query: 247 QRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVETRPIAG 306 QRL + P+ VYR LRTLNPSPYMY++ + +VG+SPE L ++ G V RPIAG Sbjct: 245 QRLCTQFHARPIDVYRALRTLNPSPYMYFIDIGATQVVGSSPEILARLQGDVVTVRPIAG 304 Query: 307 TRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFMEIERYS 366 TRPRG H+ML+DLGRND+GRV + G+V+ IERYS Sbjct: 305 TRPRGATPEHDKRLEMELLADPKERAEHVMLIDLGRNDIGRVVEAGSVQVKESFVIERYS 364 Query: 367 HVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGAYAGAIG 426 HVMHIVS V+G L G ++ D + PAGTVSGAPK+RA+EII ELE R Y+GA+G Sbjct: 365 HVMHIVSEVTGILKPGLNYVDVLRATFPAGTVSGAPKIRALEIIRELEPVCRNVYSGAVG 424 Query: 427 YLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLKAIRMA 485 Y+G+ G+ D+ I IRT + + G YVQAG G+V+DS P+ E++ET+NKA+A+ +A+ A Sbjct: 425 YIGWHGDADTAIAIRTAVIQNGCLYVQAGGGVVYDSDPDLEWQETMNKARALFRAVAQA 483 >PA0609 [EH] COG0147 Anthranilate/para-aminobenzoate synthases component I Length = 492 Score = 364 bits (935), Expect = e-100, Method: Compositional matrix adjust. Identities = 214/491 (43%), Positives = 279/491 (56%), Gaps = 28/491 (5%) Query: 8 YNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIG--SDPFLMVSX 65 YN IP+ LAD +TP+ ++ + A ++LLESV+GG +W RYS IG L V Sbjct: 14 YNRIPLSFETLADFDTPLSIYLKLADAPNSYLLESVQGGEKWGRYSIIGLPCRTVLRVYD 73 Query: 66 XXXXXXXXXXXXXRRLSGKP---VEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQY 122 R P VEE KA Y+ P + G+P F GG +G+FGYD ++Y Sbjct: 74 HQVRISIDGVETERFDCADPLAFVEEFKA---RYQVPTVPGLPRFDGGLVGYFGYDCVRY 130 Query: 123 YEKLSAHAV--DDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEE 180 EK A D L DI M D ++VFD+ L G +H S+ A Sbjct: 131 VEKRLATCPNPDPLGNPDILLMVSDAVVVFDN------LAGKIHAIVLADPSEENAYERG 184 Query: 181 LSRKLENMAEELQKEGPKENVNRRSIPQDIELGE-----IHSNLTREQYISNVEQAKEYI 235 +R E + Q P+ + D+E + ++ TRE Y + V + K+YI Sbjct: 185 QARLEELLERLRQPITPRRGL-------DLEAAQGREPAFRASFTREDYENAVGRIKDYI 237 Query: 236 RAGDIFQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVD 295 AGD QVV SQR+ IE + +P+ +YR LR NP+PYMY+ + +VG+SPE LV+V+ Sbjct: 238 LAGDCMQVVPSQRMSIEFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVE 297 Query: 296 GGRVETRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVK 355 G V RPIAGTRPRG N HLML+DLGRND+GRVS G VK Sbjct: 298 DGLVTVRPIAGTRPRGINEEADLALEQDLLSDAKEIAEHLMLIDLGRNDVGRVSDIGAVK 357 Query: 356 CDSFMEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELER 415 M IERYS+VMHIVSNV+G L EG DA + LPAGT+SGAPK+RAMEII ELE Sbjct: 358 VTEKMVIERYSNVMHIVSNVTGQLREGLSAMDALRAILPAGTLSGAPKIRAMEIIDELEP 417 Query: 416 EARGAYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKA 475 RG Y GA+GYL ++GNMD+ I IRT + + G +VQAG GIV DSVP E+EET+NK Sbjct: 418 VKRGVYGGAVGYLAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKR 477 Query: 476 KAMLKAIRMAE 486 +AM +A+ +AE Sbjct: 478 RAMFRAVALAE 488 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,461,389 Number of extensions: 1444281 Number of successful extensions: 2847 Number of sequences better than 1.0e-05: 104 Number of HSP's gapped: 2591 Number of HSP's successfully gapped: 105 Length of query: 509 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 398 Effective length of database: 25,576,357 Effective search space: 10179390086 Effective search space used: 10179390086 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)