BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0027.p (398 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0049 [S] COG4842 Uncharacterized protein conserved in bacteria 528 e-150 all3794 [E] COG0133 Tryptophan synthase beta chain 521 e-148 AGc2817 [P] COG2217 Cation transport ATPase 516 e-146 AGc2867 [R] COG1064 Zn-dependent alcohol dehydrogenases 516 e-146 AGc2804 [P] COG1121 ABC-type Mn/Zn transport systems, ATPase com... 516 e-146 >ML0049 [S] COG4842 Uncharacterized protein conserved in bacteria Length = 402 Score = 528 bits (1359), Expect = e-150, Method: Compositional matrix adjust. Identities = 252/385 (65%), Positives = 305/385 (79%) Query: 5 PDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYYA 64 P+ G +G FGG+FVPETLM A+ +LEEAY DP FQ E+ LLK Y GRE PLYYA Sbjct: 6 PNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLFQAELKELLKDYVGRENPLYYA 65 Query: 65 ERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATAT 124 +RL+++ G AKIYLKREDLNHTGAHKINNA+GQ +LAK MGK KVIAETGAGQHGVA+AT Sbjct: 66 KRLTEYAGGAKIYLKREDLNHTGAHKINNALGQVLLAKKMGKNKVIAETGAGQHGVASAT 125 Query: 125 VAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSNV 184 AAL GMEC ++MGEED +RQ+LNVFRM+LLGA+V VT GSR LKDA N ALR WV+ V Sbjct: 126 AAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKVHSVTDGSRVLKDAVNAALRAWVAQV 185 Query: 185 EDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGMF 244 EDT YV+GS +GPHP+P +VR++Q +IG E R Q LE E +LPD LVA VGGGSN++G+F Sbjct: 186 EDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARAQFLEKENKLPDALVACVGGGSNSMGLF 245 Query: 245 YPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSIS 304 YPF+ D+ V M GVEAAG G++TP HAAT++KG GV G+L +LQDE+GQ+ EA SIS Sbjct: 246 YPFVNDESVAMYGVEAAGLGIDTPHHAATITKGRPGVLHGTLMDVLQDENGQMLEAFSIS 305 Query: 305 AGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVKL 364 AGLDYPG+GPEHSY V RA+YV +TD+EALE K+L TEGIIPALES+HA+A+ VKL Sbjct: 306 AGLDYPGIGPEHSYFNAVGRAKYVDITDEEALEGFKILSRTEGIIPALESSHAIAYAVKL 365 Query: 365 GPTLTKDDLVVICLSGRGDKDVESI 389 L D +++CLSGRGDKDV + Sbjct: 366 AKELGADKSMIVCLSGRGDKDVVQV 390 >all3794 [E] COG0133 Tryptophan synthase beta chain Length = 211 Score = 521 bits (1341), Expect = e-148, Method: Compositional matrix adjust. Identities = 0/396 (0%), Positives = 0/396 (0%), Gaps = 5/396 (1%) Query: 2 IQVPDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPL 61 + +F F + + + + + G E Sbjct: 14 RENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVK 73 Query: 62 YYAERLSKHLGE-----AKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAG 116 Y + + + + + E N + + + G Sbjct: 74 TYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWP 133 Query: 117 QHGVATATVAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEA 176 V T + E + L V + + R A Sbjct: 134 TIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPR 193 Query: 177 LRYWVSNVEDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGG 236 Sbjct: 194 253 Query: 237 GSNAIGMFYPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQ 296 Sbjct: 254 313 Query: 297 VQEAHSISAGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAH 356 Sbjct: 314 373 Query: 357 ALAHVVKLGPTLTKDDLVVICLSGRGDKDVESIMAY 392 Sbjct: 374 409 >AGc2817 [P] COG2217 Cation transport ATPase Length = 218 Score = 516 bits (1329), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/388 (0%), Positives = 0/388 (0%) Query: 5 PDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYYA 64 + +A+ + + + + + + A Sbjct: 25 ILASSSASRQMLMRNAGLTFSAIPADIDERALDEQLERDGASPEEVALELARAKALAVSA 84 Query: 65 ERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATAT 124 + + + + A + A V T Sbjct: 85 LHPEALVLGCDQTMALGTRVYHKPKNMAEAATHLLSLSGKVHRLNSAAVLVHNGKVVWQT 144 Query: 125 VAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSNV 184 V++ + Q + + +GA + G+ + + Sbjct: 145 VSSAELAVRTLSAEFVSRHLQRVGEKALSSVGAYQLEREGIQLFTSIEGDYFTILGLPLL 204 Query: 185 EDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGMF 244 Sbjct: 205 264 Query: 245 YPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSIS 304 Sbjct: 265 324 Query: 305 AGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVKL 364 Sbjct: 325 384 Query: 365 GPTLTKDDLVVICLSGRGDKDVESIMAY 392 Sbjct: 385 412 >AGc2867 [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 218 Score = 516 bits (1329), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/388 (0%), Positives = 0/388 (0%) Query: 5 PDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYYA 64 + +A+ + + + + + + A Sbjct: 25 ILASSSASRQMLMRNAGLTFSAIPADIDERALDEQLERDGASPEEVALELARAKALAVSA 84 Query: 65 ERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATAT 124 + + + + A + A V T Sbjct: 85 LHPEALVLGCDQTMALGTRVYHKPKNMAEAATHLLSLSGKVHRLNSAAVLVHNGKVVWQT 144 Query: 125 VAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSNV 184 V++ + Q + + +GA + G+ + + Sbjct: 145 VSSAELAVRTLSAEFVSRHLQRVGEKALSSVGAYQLEREGIQLFTSIEGDYFTILGLPLL 204 Query: 185 EDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGMF 244 Sbjct: 205 264 Query: 245 YPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSIS 304 Sbjct: 265 324 Query: 305 AGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVKL 364 Sbjct: 325 384 Query: 365 GPTLTKDDLVVICLSGRGDKDVESIMAY 392 Sbjct: 385 412 >AGc2804 [P] COG1121 ABC-type Mn/Zn transport systems, ATPase component Length = 218 Score = 516 bits (1329), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/388 (0%), Positives = 0/388 (0%) Query: 5 PDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYYA 64 + +A+ + + + + + + A Sbjct: 25 ILASSSASRQMLMRNAGLTFSAIPADIDERALDEQLERDGASPEEVALELARAKALAVSA 84 Query: 65 ERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATAT 124 + + + + A + A V T Sbjct: 85 LHPEALVLGCDQTMALGTRVYHKPKNMAEAATHLLSLSGKVHRLNSAAVLVHNGKVVWQT 144 Query: 125 VAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSNV 184 V++ + Q + + +GA + G+ + + Sbjct: 145 VSSAELAVRTLSAEFVSRHLQRVGEKALSSVGAYQLEREGIQLFTSIEGDYFTILGLPLL 204 Query: 185 EDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGMF 244 Sbjct: 205 264 Query: 245 YPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSIS 304 Sbjct: 265 324 Query: 305 AGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVKL 364 Sbjct: 325 384 Query: 365 GPTLTKDDLVVICLSGRGDKDVESIMAY 392 Sbjct: 385 412 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,132,157 Number of extensions: 1276049 Number of successful extensions: 2747 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 2742 Number of HSP's successfully gapped: 45 Length of query: 398 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 289 Effective length of database: 25,807,025 Effective search space: 7458230225 Effective search space used: 7458230225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)