BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0016.p (168 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0211 [R] COG0663 Carbonic anhydrases/acetyltransferases, isole... 184 4e-47 BH3289 [R] COG0663 Carbonic anhydrases/acetyltransferases, isole... 172 2e-43 MA1134 [R] COG0663 Carbonic anhydrases/acetyltransferases, isole... 153 1e-37 mlr0180 [R] COG0663 Carbonic anhydrases/acetyltransferases, isol... 149 2e-36 PA3753 [R] COG0663 Carbonic anhydrases/acetyltransferases, isole... 144 7e-35 >BH0211 [R] COG0663 Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily Length = 175 Score = 184 bits (468), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 85/167 (50%), Positives = 121/167 (72%) Query: 1 MRIGYGNYIPQLDESVYVAEGAKLVGDVRIGRQSSVWFNAVLRGDLAPVIIGDRCNIQDG 60 M Y ++ P++D + ++AE A ++G+V IG ++V FNAV+RGD + IG RCNIQ+ Sbjct: 1 MIFRYKDFSPKVDTTAFIAESATVIGNVEIGAYTTVLFNAVIRGDEGLIKIGRRCNIQEN 60 Query: 61 TVGHVAEGLPLVLGDDISVGHSAIIHGCRIGKGTLIGMGAIVLNGAEIGEYALIGAGSIV 120 + H+ E PLVL D++S+GH AI+HGC + +G LIGMGA VL+GAEIGEY+++GAGS+V Sbjct: 61 VMCHLYEQYPLVLEDEVSLGHHAIVHGCTLKQGVLIGMGATVLDGAEIGEYSIVGAGSVV 120 Query: 121 TENKIIPPYTLSLGSPARVIRELTEQDLQRMARTCESYVLKASEYGR 167 K+IPP +L LGSP +V+RE+TEQDL + T E+YV E+ R Sbjct: 121 PPGKVIPPRSLVLGSPGKVVREVTEQDLAMIEETVETYVRNGQEFKR 167 >BH3289 [R] COG0663 Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily Length = 174 Score = 172 bits (436), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 81/168 (48%), Positives = 115/168 (68%) Query: 1 MRIGYGNYIPQLDESVYVAEGAKLVGDVRIGRQSSVWFNAVLRGDLAPVIIGDRCNIQDG 60 M Y + P++ E+V++A+ + GDV IG SS+W+N V+RGD++P IG+R NIQD Sbjct: 1 MLYPYKDKKPKIAENVFLADYVTITGDVTIGADSSIWYNTVIRGDVSPTFIGERVNIQDN 60 Query: 61 TVGHVAEGLPLVLGDDISVGHSAIIHGCRIGKGTLIGMGAIVLNGAEIGEYALIGAGSIV 120 +V H + PLV+ DD++VGH I+H C I K LIGMG+I+L+GAE+GE A IGAGS+V Sbjct: 61 SVLHQSPRTPLVIEDDVTVGHQVILHSCTIRKEALIGMGSIILDGAEVGEGAFIGAGSLV 120 Query: 121 TENKIIPPYTLSLGSPARVIRELTEQDLQRMARTCESYVLKASEYGRI 168 + K IP +L+ G PA+V+R LTE+D + MAR YV K Y ++ Sbjct: 121 PQGKKIPANSLAFGRPAKVVRTLTEEDRKDMARIRREYVEKGQYYKQV 168 >MA1134 [R] COG0663 Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily Length = 181 Score = 153 bits (386), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 71/165 (43%), Positives = 106/165 (64%) Query: 1 MRIGYGNYIPQLDESVYVAEGAKLVGDVRIGRQSSVWFNAVLRGDLAPVIIGDRCNIQDG 60 MRI + P++ E+ +VA A ++G+V + SS+WFNAV+RGD + IG+R +IQDG Sbjct: 1 MRIYFNGKTPKISETAFVANSADIIGNVEVESFSSIWFNAVIRGDQNKIKIGNRTSIQDG 60 Query: 61 TVGHVAEGLPLVLGDDISVGHSAIIHGCRIGKGTLIGMGAIVLNGAEIGEYALIGAGSIV 120 V H + +GD++SVGH A++HGCRI LIGM A VLNGAEIG+ +++GA ++V Sbjct: 61 VVIHADPENGVQIGDNVSVGHGAVLHGCRIEDNVLIGMNATVLNGAEIGKNSIVGANALV 120 Query: 121 TENKIIPPYTLSLGSPARVIRELTEQDLQRMARTCESYVLKASEY 165 + K PP +L +G P + RE E +++ + + YV A EY Sbjct: 121 PQGKKFPPNSLIIGVPGTIKRETNEAEVEAIKENADEYVEMAKEY 165 >mlr0180 [R] COG0663 Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily Length = 301 Score = 149 bits (377), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 13/151 (8%), Positives = 31/151 (20%) Query: 15 SVYVAEGAKLVGDVRIGRQSSVWFNAVLRGDLAPVIIGDRCNIQDGTVGHVAEGLPLVLG 74 +V + + +I S R + D H+ E + Sbjct: 18 NVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRL 77 Query: 75 DDISVGHSAIIHGCRIGKGTLIGMGAIVLNGAEIGEYALIGAGSIVTENKIIPPYTLSLG 134 + + S I V + + L Sbjct: 78 MNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLP 137 Query: 135 SPARVIRELTEQDLQRMARTCESYVLKASEY 165 + ++ D+ ++ V + Sbjct: 138 HLQFLASQMNFLDIVPISAETGLNVDTIAAI 168 >PA3753 [R] COG0663 Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily Length = 174 Score = 144 bits (362), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 69/167 (41%), Positives = 107/167 (64%) Query: 1 MRIGYGNYIPQLDESVYVAEGAKLVGDVRIGRQSSVWFNAVLRGDLAPVIIGDRCNIQDG 60 M+ G+ + ++A A ++G VR+ +SVWF AVLRGD + IG+ N+QDG Sbjct: 1 MKYRLGDARVETHPDSWIAPSAAVIGKVRLDAGASVWFGAVLRGDNELIHIGEHSNVQDG 60 Query: 61 TVGHVAEGLPLVLGDDISVGHSAIIHGCRIGKGTLIGMGAIVLNGAEIGEYALIGAGSIV 120 +V H G PL LG ++VGH+A++HGC +G +L+G+ A++LNGA+IG+Y +IGA +++ Sbjct: 61 SVMHTDMGYPLTLGKGVTVGHNAMLHGCSVGDYSLVGINAVILNGAKIGKYCIIGANALI 120 Query: 121 TENKIIPPYTLSLGSPARVIRELTEQDLQRMARTCESYVLKASEYGR 167 E K IP +L +GSP +V+REL+E + + + YV A Y R Sbjct: 121 PEGKEIPDGSLVMGSPGKVVRELSEPQKKMLEASAAHYVHNARRYAR 167 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.141 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,420,547 Number of extensions: 598846 Number of successful extensions: 1541 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 1482 Number of HSP's successfully gapped: 67 Length of query: 168 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 69 Effective length of database: 26,960,365 Effective search space: 1860265185 Effective search space used: 1860265185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 112 (47.8 bits)