BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0013.p (164 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_qcrA [C] COG0723 Rieske Fe-S protein 159 2e-39 BH1672 [C] COG0723 Rieske Fe-S protein 145 4e-35 sll1316 [C] COG0723 Rieske Fe-S protein 63 2e-10 all4511 [C] COG0723 Rieske Fe-S protein 62 3e-10 all2453 [C] COG0723 Rieske Fe-S protein 60 1e-09 >BS_qcrA [C] COG0723 Rieske Fe-S protein Length = 167 Score = 159 bits (402), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 74/161 (45%), Positives = 102/161 (63%), Gaps = 3/161 (1%) Query: 1 MSRRQFLTYTLGGATAFMGAGAILPMIRFAVDPVLHKKGAGEFIKVAETAKITDVPQEFT 60 +SRRQFL YTL G FM A ++PM+RFA+DPVL G + ++V ++T PQ F Sbjct: 7 ISRRQFLNYTLTGVGGFMAASMLMPMVRFALDPVLKSTGKQDMVQVVSVDELTKEPQRFD 66 Query: 61 FELPQQDGWYASTATLTAWIRKDENGEIYALSPICKHLGCTVGWNNDKAYPDEYHCPCHG 120 F++ Q D WY S + +AW+ K+ + EI ALSPICKHLGCTV WN+D P+++ CPCH Sbjct: 67 FKINQVDAWYESEESRSAWVFKNGD-EIVALSPICKHLGCTVNWNSDPKNPNKFFCPCHY 125 Query: 121 ARYTKIGKNLAVAP--KPLDQYKTKIDGDWVYLGEIVPNTE 159 Y K G N+ P PLD Y+ ++ ++YLG+ P E Sbjct: 126 GLYEKDGTNVPGTPPLAPLDHYEQEVKDGFLYLGKAKPKGE 166 >BH1672 [C] COG0723 Rieske Fe-S protein Length = 166 Score = 145 bits (365), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 67/159 (42%), Positives = 95/159 (59%), Gaps = 3/159 (1%) Query: 1 MSRRQFLTYTLGGATAFMGAGAILPMIRFAVDPVLHKKGAGEFIKVAETAKITDVPQEFT 60 +SRRQFLTY+L G FM A A LPM+RFA+DP L E + + +T+ PQ F Sbjct: 8 VSRRQFLTYSLMGVGGFMAAAASLPMVRFALDPALKAGAETELHYICDLDDLTEEPQSFN 67 Query: 61 FELPQQDGWYASTATLTAWIRKDENGEIYALSPICKHLGCTVGWNNDKAYPDEYHCPCHG 120 + QQD WY S A T ++ + E+ ALS C HLGCTV + ++ +PD++ CPCH Sbjct: 68 LQYAQQDAWYESEANHTVYLFR-HGEEVVALSSTCTHLGCTVAFGTNEDHPDQFFCPCHF 126 Query: 121 ARYTKIGKNLAVAP--KPLDQYKTKIDGDWVYLGEIVPN 157 R+ K G N+ P +PLD+Y+ ++ VYLG ++ N Sbjct: 127 GRFEKDGTNVPNLPPQRPLDRYEVSVEDGKVYLGSLIQN 165 >sll1316 [C] COG0723 Rieske Fe-S protein Length = 192 Score = 62.8 bits (151), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 40/145 (27%), Positives = 64/145 (44%), Gaps = 19/145 (13%) Query: 1 MSRRQFLTYTLGGATAFMGAGAILPMIRFAVDP--------VLHKKGAGEFIKVAETAKI 52 + RRQF+ G + AGA+ P +++ + P V K G +KV E Sbjct: 25 LGRRQFMNLLTFGTITGVAAGALYPAVKYLIPPSSGGSGGGVTAKDALGNDVKVTEFLAS 84 Query: 53 TDVPQEFTFELPQQDGWYASTATLTAWIRKDENGEIYALSPICKHLGCTVGWNNDKAYPD 112 + + + D Y ++ D+ Y ++ +C HLGC V WN A + Sbjct: 85 HNAGDRVLAQGLKGDPTYIV-------VQGDDTIANYGINAVCTHLGCVVPWN---ASEN 134 Query: 113 EYHCPCHGARYTKIGKNLAVAPKPL 137 ++ CPCHG++Y GK + P PL Sbjct: 135 KFMCPCHGSQYNAEGK-VVRGPAPL 158 >all4511 [C] COG0723 Rieske Fe-S protein Length = 178 Score = 62.4 bits (150), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 43/157 (27%), Positives = 65/157 (41%), Gaps = 13/157 (8%) Query: 1 MSRRQFLTYTLGGATAFMGAGAILPMIRFAVDPVLHKKGAGEFIKVAETAKITDVPQEFT 60 MSRRQ L + G A + AI P +F + P G + + P + Sbjct: 12 MSRRQLLNFFTGAIVATTASAAIYPATKFFMPPAESTDAEGGILAQDKIGH----PIPAS 67 Query: 61 FELPQQDGWYASTATLTA-----WIRKDENGEIYALSPICKHLGCTVGWNNDKAYPDEYH 115 L Q G A A L +R+D + + C HLGCT WN ++ Sbjct: 68 QILAQASGTRALIAGLAGEPTYLTVREDGTLDPMGIVNNCTHLGCTFPWN---PVDQQFQ 124 Query: 116 CPCHGARYTKIGK-NLAVAPKPLDQYKTKIDGDWVYL 151 CPCHG+RY G A +PL + ++ D++++ Sbjct: 125 CPCHGSRYDAQGSVERGPANRPLKLVQVQVKDDYIWI 161 >all2453 [C] COG0723 Rieske Fe-S protein Length = 179 Score = 60.1 bits (144), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 42/160 (26%), Positives = 68/160 (42%), Gaps = 19/160 (11%) Query: 1 MSRRQFLTYTLGGATAFMGAGAILPMIRFAVDPVLH--------KKGAGEFIKVAETAKI 52 M RRQF+ G + GA+ P++ + + P K G + V++ + Sbjct: 13 MGRRQFMNLLTFGTVTGVALGALYPVVNYFIPPAAGGAGGGTTAKDELGNDVSVSKFLES 72 Query: 53 TDVPQEFTFELPQQDGWYASTATLTAWIRKDENGEIYALSPICKHLGCTVGWNNDKAYPD 112 +V + + D Y + E Y ++ +C HLGC V WN A + Sbjct: 73 HNVGDRTLVQGLKGDPTYIV-------VESKEAITDYGINAVCTHLGCVVPWN---AAEN 122 Query: 113 EYHCPCHGARYTKIGKNL-AVAPKPLDQYKTKIDGDWVYL 151 ++ CPCHG++Y GK + APK L K + D + L Sbjct: 123 KFKCPCHGSQYDATGKVVRGPAPKSLALSHAKTENDKIVL 162 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,185,842 Number of extensions: 552957 Number of successful extensions: 766 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 757 Number of HSP's successfully gapped: 11 Length of query: 164 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 66 Effective length of database: 27,075,699 Effective search space: 1786996134 Effective search space used: 1786996134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)