BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0007.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2023 [S] COG1284 Uncharacterized conserved protein 245 6e-65 BH1678 [S] COG1284 Uncharacterized conserved protein 240 2e-63 BS_ypjC [S] COG1284 Uncharacterized conserved protein 198 6e-51 BS_yxkD [S] COG1284 Uncharacterized conserved protein 193 3e-49 SPy2153 [S] COG1284 Uncharacterized conserved protein 176 6e-44 >lin2023 [S] COG1284 Uncharacterized conserved protein Length = 288 Score = 245 bits (626), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 129/276 (46%), Positives = 178/276 (64%), Gaps = 5/276 (1%) Query: 13 KTAAPIMLGAAVYAFGLLYFIIPNQLMEGGVTGITILLNYAFXXXXXXXXXXXXXXXXXX 72 K IMLG A+YAFGL+ F I N L EGG+ G+T+ L + F Sbjct: 8 KNICFIMLGTAIYAFGLVNFNIANNLGEGGLAGVTLFLLHFFQIDPAYSNLVLNIPLFII 67 Query: 73 GWKVLGGRQIAYTGVGIGALSLFLWLFERMIDAGWIVTFSTEHDFILASLYAGVTLGLGL 132 GW+VLG R + YTG+G +LSLFLW+F+R+ T D +L +L+AG G+GL Sbjct: 68 GWRVLGSRSLIYTGIGTVSLSLFLWIFQRIP-----YTLDLHSDLLLVALFAGGFSGIGL 122 Query: 133 GIVFRFGGTTGGVDIVARILGRKFGWSMGQIILAVDVIIIGASLLYIPREKILYTLVAVF 192 G+VFR+GGTTGG DI+A++L G SMG+ + A+D I++ ASL Y+ +++YTLVAVF Sbjct: 123 GLVFRYGGTTGGSDIIAKLLNHTKGISMGRTLFAIDAIVLAASLSYLDVRQVMYTLVAVF 182 Query: 193 ISSRVIDFIQEGAYAAKAFTIISDDAPQIADLITAEMDRGVTLIPAIGAYSKQAKYMVYC 252 I SRVIDF+QEGAYAA+ IIS D IA + M+RGVT++ G +SK + ++Y Sbjct: 183 IGSRVIDFVQEGAYAARGALIISKDNDAIASHVMLSMNRGVTVLEGRGGFSKNEQDVLYI 242 Query: 253 VVSRQEIRRLSQLVKSVDAKAFVIISDVHDVHGEGF 288 VV++ EI +L +V+++D AFV +S VHDV GEGF Sbjct: 243 VVAKNEIVQLKNIVQAIDPHAFVSVSVVHDVMGEGF 278 >BH1678 [S] COG1284 Uncharacterized conserved protein Length = 290 Score = 240 bits (612), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 126/277 (45%), Positives = 179/277 (64%), Gaps = 6/277 (2%) Query: 13 KTAAPIMLGAAVYAFGLLYFIIPNQLMEGGVTGITILLNYAFXXXXXXXXXXXXXXXXXX 72 K I+LG+A+++FGL+YF + N L EGG TGIT++L + F Sbjct: 8 KNIVFILLGSAIFSFGLVYFNMENNLAEGGFTGITLILYFMFQINPAVTNLVLNIPILLI 67 Query: 73 GWKVLGGRQIAYTGVGIGALSLFLWLFERMIDAGW-IVTFSTEHDFILASLYAGVTLGLG 131 GWK+LG + YT +G ++S+FL +F+R W + D LA+L+AGV +G G Sbjct: 68 GWKILGRVTLIYTIIGTVSVSVFLEMFQR-----WKFMDIPLHDDMTLAALFAGVFVGTG 122 Query: 132 LGIVFRFGGTTGGVDIVARILGRKFGWSMGQIILAVDVIIIGASLLYIPREKILYTLVAV 191 LGIVFRFGGTTGGVDI+A++ R GWSMG+ + D ++I +SL+Y+ + +YTL+AV Sbjct: 123 LGIVFRFGGTTGGVDIIAKLGFRYLGWSMGKTMFMFDAVVIASSLIYLNYREAMYTLLAV 182 Query: 192 FISSRVIDFIQEGAYAAKAFTIISDDAPQIADLITAEMDRGVTLIPAIGAYSKQAKYMVY 251 FI+++VIDFIQ+ AY+AKA IIS+ IAD I EM+RG T + G+++ K ++Y Sbjct: 183 FIAAKVIDFIQQTAYSAKAAFIISEHTEAIADTILKEMERGATTLKGKGSFTGTEKEILY 242 Query: 252 CVVSRQEIRRLSQLVKSVDAKAFVIISDVHDVHGEGF 288 CVV R E+ RL LV+ +D AFV ++DV DV GEGF Sbjct: 243 CVVGRNELIRLKSLVERIDPHAFVTVNDVQDVIGEGF 279 >BS_ypjC [S] COG1284 Uncharacterized conserved protein Length = 160 Score = 198 bits (504), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 0/206 (0%), Positives = 0/206 (0%), Gaps = 6/206 (2%) Query: 84 YTGVGIGALSLFLWLFERMIDAGWIVTFSTEHDFILASLYAGVTLGLGLGIVFRFGGTTG 143 G A ++ + + L Sbjct: 4 SKSNGFNASAVLGSGSYN-----ENKSSKHMELLAHSILKLICKEAASETYRGALETLQK 58 Query: 144 GVDIVARILGRKFGWSMGQIILAVDVIIIGASLL-YIPREKILYTLVAVFISSRVIDFIQ 202 + G F L +G + L + VI + Sbjct: 59 MMSECIYQEGNAFVIMGAGEQLKRIKYEVGENNLKVFNVHFNNNHELVSSGEPDVICLSK 118 Query: 203 EGAYAAKAFTIISDDAPQIADLITAEMDRGVTLIPAIGAYSKQAKYMVYCVVSRQEIRRL 262 Sbjct: 119 178 Query: 263 SQLVKSVDAKAFVIISDVHDVHGEGF 288 Sbjct: 179 204 >BS_yxkD [S] COG1284 Uncharacterized conserved protein Length = 278 Score = 193 bits (490), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 104/271 (38%), Positives = 164/271 (60%), Gaps = 8/271 (2%) Query: 18 IMLGAAVYAFGLLYFIIPNQLMEGGVTGITILLNYAFXXXXXXXXXXXXXXXXXXGWKVL 77 +++GA +A + F IPN L EGGVTGIT++L Y F G+K L Sbjct: 11 LVIGAFFFALAVNLFAIPNDLGEGGVTGITLILYYLFQWSPGVTNFILNAFLLLIGYKFL 70 Query: 78 GGRQIAYTGVGIGALSLFLWLFERMIDAGWIVTFSTEHDFILASLYAGVTLGLGLGIVFR 137 G+ YT + + A SLFL L GW + + I+ +++AGV G+G+G++ R Sbjct: 71 DGKTTVYTIIAVAANSLFLHL-----THGWSIP---SDELIINTIFAGVFAGVGIGMIIR 122 Query: 138 FGGTTGGVDIVARILGRKFGWSMGQIILAVDVIIIGASLLYIPREKILYTLVAVFISSRV 197 GGTT G I+ARI + W++ +L D+I++ +S I EK+++T+V ++I ++V Sbjct: 123 VGGTTAGSAILARIANKYLDWNISYALLFFDLIVVFSSYFIIGAEKMMFTIVMLYIGTKV 182 Query: 198 IDFIQEGAYAAKAFTIISDDAPQIADLITAEMDRGVTLIPAIGAYSKQAKYMVYCVVSRQ 257 +DFI EG KA T+IS++ +IA+ + MDRGVT++ G Y+ Q+K ++Y V+++Q Sbjct: 183 MDFIIEGLNTKKAITVISENKSEIAEQVNTLMDRGVTILSGKGNYTGQSKEILYIVINKQ 242 Query: 258 EIRRLSQLVKSVDAKAFVIISDVHDVHGEGF 288 E+ L ++++S D KAFVI+ DV DV GEGF Sbjct: 243 ELSMLKKIIRSCDKKAFVIVHDVRDVFGEGF 273 >SPy2153 [S] COG1284 Uncharacterized conserved protein Length = 290 Score = 176 bits (445), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 98/272 (36%), Positives = 153/272 (56%), Gaps = 5/272 (1%) Query: 18 IMLGAAVYAFGLLYFIIPNQLMEGGVTGITILLNYAFXXXXXXXXXXXXXXXXXXGWKVL 77 I LG A+Y FG + F + N L EGGV GIT++L+ F G K+ Sbjct: 16 IALGVAIYTFGFVNFNMANALAEGGVAGITLILHAHFGINPAYSSLLFNLPLFILGAKIF 75 Query: 78 GGRQIAYTGVGIGALSLFLWLFERMIDAGWIVTFSTEHDFILASLYAGVTLGLGLGIVFR 137 G R +A T G +S F+W+++++ + E+D +L ++ AG+ G+G GIVFR Sbjct: 76 GKRSLALTIYGTVLMSAFIWMWQKVP-----IELGLENDMMLVAVVAGLFSGIGSGIVFR 130 Query: 138 FGGTTGGVDIVARILGRKFGWSMGQIILAVDVIIIGASLLYIPREKILYTLVAVFISSRV 197 +G TTGG DI+ RI KFG +GQ +L VD +++ ASL Y+ + +LYTLVA F+ S++ Sbjct: 131 YGATTGGTDIIGRIAEEKFGAKLGQTLLLVDALVLTASLTYVDLKHMLYTLVASFVFSQM 190 Query: 198 IDFIQEGAYAAKAFTIISDDAPQIADLITAEMDRGVTLIPAIGAYSKQAKYMVYCVVSRQ 257 I +Q G Y + II+ + A I E++RGVT + GAYS ++Y ++ Sbjct: 191 ISVVQNGGYTIRGMIIITKHSEAAAQAILTEINRGVTYLKGQGAYSGNDYNIMYVTLNPT 250 Query: 258 EIRRLSQLVKSVDAKAFVIISDVHDVHGEGFR 289 E+R + +++ +D AF+ I DV +V F+ Sbjct: 251 EVREVKRILAGLDPDAFISIIDVDEVISSDFK 282 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.143 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,375,569 Number of extensions: 766536 Number of successful extensions: 2415 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 2359 Number of HSP's successfully gapped: 35 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)