BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0008.p (298 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy1297 [K] COG1609 Transcriptional regulators 78 1e-14 lin1838 [K] COG1609 Transcriptional regulators 72 1e-12 TM1200 [K] COG1609 Transcriptional regulators 68 1e-11 Cgl1332 [K] COG1609 Transcriptional regulators 66 5e-11 CAC0360 [K] COG1609 Transcriptional regulators 62 2e-09 >SPy1297 [K] COG1609 Transcriptional regulators Length = 266 Score = 78.2 bits (191), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 61/172 (35%), Positives = 91/172 (52%), Gaps = 16/172 (9%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KDIA A VSVATVSYVLN+ ++Q I E+TR ++L ++ L+Y PN AARSL Q++ Sbjct: 8 IKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHISNLLNYTPNQAARSLVTQQSNS 67 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIVERKLEAV 120 V + Y+K + + EQ T + LL+ D L+ K + Sbjct: 68 VVL---------YYKYADSI--LARAEQLHTISFLSRLLHEQDYQLVCLNEDSTEKFDWA 116 Query: 121 FLI---DAQDASFYSISRHFVEGIPLILIDSMIEDKLFKQVVYNYRSALEKA 169 LI D + +FY I + + PLI IDS + D LF Q+ +Y + ++A Sbjct: 117 DLILTLDVDNDTFYKIGK--LNFKPLIAIDSFVNDPLFFQINTDYAALKDQA 166 >lin1838 [K] COG1609 Transcriptional regulators Length = 321 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 43/126 (34%), Positives = 72/126 (57%), Gaps = 11/126 (8%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KDIA +A VS++TVSY LN + + EKTR++I+ +A EL+YIPN+AAR+L ++T + Sbjct: 4 IKDIAKKAGVSISTVSYALN--GSPKVTEKTRKRIMTIANELNYIPNMAARTLKTRETKI 61 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIVERKLEAV 120 +GV + + Y L + L + L GY + S G + + E+ ++ Sbjct: 62 IGVYLTNYGGIFYGTL------LEGLTKTLYKHGYELIACS---GTKTHRFLPEKMIDGA 112 Query: 121 FLIDAQ 126 ++DA Sbjct: 113 IILDAN 118 >TM1200 [K] COG1609 Transcriptional regulators Length = 333 Score = 68.2 bits (165), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 45/155 (29%), Positives = 82/155 (52%), Gaps = 24/155 (15%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 ++DIA +A VS+ TVS LN + I E+TRR+IL++A+EL Y+ N A SL +++T + Sbjct: 8 IRDIAEKAGVSINTVSRALN--NKPDISEETRRKILKIAQELGYVKNATASSLRSKQTNI 65 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSL----DTGNPSLDIIVERK 116 VGV++ S P++ + +E GY +L + + +++++++R+ Sbjct: 66 VGVIIADSAN-PFYA-----EVLKGIEAASRKYGYQIMLMNTERIYENEEKAIEVLLQRR 119 Query: 117 LEAVFLIDAQDAS------------FYSISRHFVE 139 ++ + + QD S F + RHF E Sbjct: 120 VDGLLITPVQDRSDDIKTLIERKVPFVIVGRHFEE 154 >Cgl1332 [K] COG1609 Transcriptional regulators Length = 346 Score = 66.2 bits (160), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 67/274 (24%), Positives = 124/274 (45%), Gaps = 40/274 (14%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KD+A +A VS+AT S L DN + TR +I QLA +L Y N AR+L + ++ Sbjct: 10 LKDVARQAGVSIATASRAL--ADNPAVAASTRERIQQLASDLGYRANAQARALRSSRSNT 67 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLD----TGNPSLDIIVERK 116 +GV+V P H A + ++ + AG T++ + + T + SL+ + Sbjct: 68 IGVIV------PSLINHYFAAMVTEIQSTASKAGLATIITNSNEDATTMSGSLEFLTSHG 121 Query: 117 LEAVFLIDAQDASFYSISRHFVEGIPLILID-SMIEDKLFKQVVYNYRSALEKALPDDLS 175 ++ + + ++ + + +G+P++L+D + D N + + A+ Sbjct: 122 VDGIICVPNEECA-NQLEDLQKQGMPVVLVDRELPGDSTIPTATSNPQPGIAAAVE---- 176 Query: 176 QVCLIMESFNNSLLTRYIRSCVPLSDDAVFEIRELQDLLSATAHHRFKQAIVINEFIGSY 235 + + NN+L Y+ P+ D L+D +A A+ + + +V F+G Y Sbjct: 177 -----LLAHNNALPIGYLSG--PM--DTSTGRERLEDFKAACANSKIGEQLV---FLGGY 224 Query: 236 VEKSGDFEHLTVICTCNSPQMLGERVEKILFQGD 269 E+S FE T + ++ K LF GD Sbjct: 225 -EQSVGFEGATKLL---------DQGAKTLFAGD 248 >CAC0360 [K] COG1609 Transcriptional regulators Length = 328 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 44/154 (28%), Positives = 78/154 (50%), Gaps = 18/154 (11%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KDIA VS TVS LN D+ I E T+ +IL++ EEL Y PN AA+SL +KT + Sbjct: 4 IKDIARYCGVSAMTVSRALNNSDD--IAEATKEKILKVCEELKYKPNSAAKSLITKKTNM 61 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLDTGNPS----LDIIVERK 116 +G+++ Y + ++ + GY +L + + P+ LD + E + Sbjct: 62 IGLIIPDIANQYYANISKGVS------SYIEKHGYGLILCNSNRKKPNEVKYLDFLSEGR 115 Query: 117 LEAVFL--IDAQDASFYSISRHFVEGIPLILIDS 148 ++ + + + + + ISR IP+++ D+ Sbjct: 116 VDGIIILPVKPRKEDYEDISRQ----IPMVMADN 145 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,708,337 Number of extensions: 809897 Number of successful extensions: 1554 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 1586 Number of HSP's successfully gapped: 59 Length of query: 298 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 192 Effective length of database: 26,153,027 Effective search space: 5021381184 Effective search space used: 5021381184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)