BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0002.p (382 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH341 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit 109 6e-24 MA0978 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit 98 2e-20 MJ0032 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit 96 6e-20 MK0539 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit 87 4e-17 MA3732 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit 82 1e-15 >MTH341 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit Length = 254 Score = 109 bits (273), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 0/250 (0%), Positives = 0/250 (0%), Gaps = 10/250 (4%) Query: 28 CNLCSEVCPGYDTGVMESERRIFGRNRSELERWTGIYLSTHQFSAADPEILGRAAAGGAG 87 + + + + R ++ A P + Sbjct: 48 PDPMAGKRTSAQSYGAGRGVAMVPRIKNGS--RAAFVPQAVGGRRAHPPRPQKNYHERIN 105 Query: 88 TILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSYCITPNLDLLQD- 146 A+ +A + + V T + Sbjct: 106 RKERRLAIRSAVAATARKDLVEARGHRIENVPQLPLVVDDELSMIKRTADTREVFRKLGI 165 Query: 147 -DRYDKIGIIGVPCQIQGIHKLLNLPEHLPSSVLADKVAFTIELGCASSTSLGGTEHLIT 205 D + +G + + + + G ++ Sbjct: 166 MDDIVRAREGKKIRAGKGKMR-----GRKYRTPRGPLIVVGDDKGITRGARNHPGVDVV- 219 Query: 206 EILGIELADVAVMRYREGQYPGQFMVKTRQGQEHYLPFYRLVEEFKKFKTFRCLACPDWW 265 Sbjct: 220 279 Query: 266 SGIADISISD 275 Sbjct: 280 289 >MA0978 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit Length = 291 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 76/257 (29%), Positives = 120/257 (46%), Gaps = 28/257 (10%) Query: 60 WTGIYLSTHQFSAADPEILGRAAAGGAGTILAVTALEEKLADAVIVVGRDEERPWVPKAY 119 + G Y + + D EIL +A GG T L V ALEE + D VIV G + + PW P+ + Sbjct: 6 YLGKYTTCISARSTDREILKKAQDGGIATTLMVYALEEGIIDGVIVAG-EGDLPWKPRPF 64 Query: 120 LADSVERIIECAQTSYCITPNLDLLQDDR----YDKIGIIGVPCQIQGIHKLLNLPEHLP 175 +A S + I++ + Y I+P + +++ DK+G+ GV CQIQ + K P ++ Sbjct: 65 VAMSRKDILKAQKAKYNISPQISWIKEATRSFGLDKVGVTGVCCQIQAVRKAQLYPINMR 124 Query: 176 SSVLADKVAFTIELGCASSTSLGGTEHLITEILGIELADVAVMRYREGQYPGQFMVKTRQ 235 + K+AF + L C + + ++ + L+ V MR + G+F V T + Sbjct: 125 D--VPGKIAFVVGLFCMENFLYDSIQAIVEDHTNQSLSSVKKMRIGK----GKFQVYTER 178 Query: 236 GQEHYLPFYRLVEEFKKFKTFRCLACPDWWSGIADISI-SDGDPNIFDSSKEGISAKASS 294 G P + K+ C C D+ S + DIS S G P+ + S Sbjct: 179 GNVATAPL----QATHKYGQPGCYVCLDYVSNLGDISTGSVGSPDGW------------S 222 Query: 295 TVMVRTKTGARLLELAV 311 TV VRT+ G + AV Sbjct: 223 TVFVRTRVGNDIWSKAV 239 >MJ0032 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit Length = 287 Score = 96.3 bits (238), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 73/244 (29%), Positives = 115/244 (47%), Gaps = 35/244 (14%) Query: 76 EILGRAAAGGAGTILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSY 135 E+L +A GG + + LE L D VIV E VPK +A + E ++E A T Y Sbjct: 24 EVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEFKAVPK--VATTPEEVLEAAGTKY 81 Query: 136 CITPNLDLLQDD--RY--DKIGIIGVPCQIQGIHKLLNLP---EHLPSSVLADKVAFTIE 188 + PN+ +L+ Y +KIG++G PCQ++ + KL+ P H+P DK+A I Sbjct: 82 TVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKYPVGFRHVP-----DKIALIIG 136 Query: 189 LGCASSTSLGGTEHLITEILGIELADVAVMRYREGQYPGQFMVKTRQGQEHYLPFYRLVE 248 + C + G + ++ E G+++ DV M + G+F V TR G+ + + Sbjct: 137 IFCMENFPYYGLKLIVEEHCGVKMEDVVKMDIGK----GKFWVYTRWGETKAIKL----K 188 Query: 249 EFKKFKTFRCLACPDWWSGIADISI-SDGDPNIFDSSKEGISAKASSTVMVRTKTGARLL 307 E ++ C C D+ + +ADIS S G P+ STV +RT G + Sbjct: 189 ETHPYEQIACHVCTDYTAELADISTGSVGSPD------------GWSTVFIRTAKGEEIF 236 Query: 308 ELAV 311 V Sbjct: 237 NKMV 240 >MK0539 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit Length = 275 Score = 87.4 bits (215), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 81/296 (27%), Positives = 131/296 (44%), Gaps = 32/296 (10%) Query: 62 GIYLSTHQFSAADPEILGRAAAGGAGTILAVTALEEKLADAVIVVGRDEERPWVPKAYLA 121 G Y + A D I GG T L ALEE +AVI E W P+ + Sbjct: 2 GEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVE--TWKPEPVIV 59 Query: 122 DSVERIIECAQTSYCITPNLDLLQD--DRYDKIGIIGVPCQIQGIHKLLNLPEHLPSSVL 179 + +IE A + Y I+PN+ L + YD + ++G PCQI + K P L + + Sbjct: 60 TDPDELIETAGSKYAISPNVSALNEAIASYDSVALVGTPCQITAVKKSKMYPYGLAN--V 117 Query: 180 ADKVAFTIELGCASSTSLGGTEHLITEILGIELADVAVMRYREGQYPGQFMVKTRQGQEH 239 ++V T+ + C + L+ E +G+++ +V M G+F+V+T+ G H Sbjct: 118 TERVKLTVGIFCTENFQYESLLKLL-EDMGVDVENVERMDISH----GEFIVRTKNGDVH 172 Query: 240 YLPFYRLVEEFKKFKTFRCLACPDWWSGIADISI-SDGDPNIFDSSKEGISAKASSTVMV 298 + +L + C C D+ + ADIS+ S G P+ ++ V+V Sbjct: 173 SVSVSKL----GDYANEACNYCTDFTAEDADISVGSVGAPDGWN------------VVLV 216 Query: 299 RTKTGARLLELAVRRNAARLVDY-TFDNNLGLER--KRQRYRSYAAMGDRRIPLAP 351 RTK G ++ AV + + D D NL LER + ++ R + +M P P Sbjct: 217 RTKEGEKVFRSAVDADVLEVKDIGEGDPNL-LERLARDKKERIHTSMCATWRPYHP 271 >MA3732 [C] COG1035 Coenzyme F420-reducing hydrogenase, beta subunit Length = 346 Score = 82.4 bits (202), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 81/291 (27%), Positives = 126/291 (43%), Gaps = 60/291 (20%) Query: 28 CNLCSEVCPGYDTGVMESE----RRIFGRNRSELERWTGIYLSTHQFSAADPEILGRAAA 83 C CS VCP D G ++ E R+ FG A + +G Sbjct: 58 CLTCSRVCPVVD-GFIQDELTNVRKFFG--------------------ARSKDNVGSQDG 96 Query: 84 GGAGTILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSYCITPNLDL 143 G A IL + K+ D + + R+E+ W P+ L S E + T Y P L Sbjct: 97 GVASGILKSLFKQGKI-DCAVGITRNEK--WEPEVVLLTSAEDVERTRGTKYTSDPVLAA 153 Query: 144 LQD--DRYDKIGIIGVPCQIQGIHKLLNLPEHLPSSVLADKVAFTIELGCASSTSLGGT- 200 L++ ++YD+I +IGVPCQ HL + +K+ I L C S Sbjct: 154 LREAFEKYDRIAVIGVPCQAHA--------AHLIRENVNEKIVLIIGLLCMESFHHDVML 205 Query: 201 EHLITEILGIELADVAVMRYREGQYPGQFMVKTRQGQEHYLPFYRLVEEFKKFKTFRCLA 260 E +I EIL ++L D+ M + + G+F V T+ G+ H +P +++ KF C Sbjct: 206 EKIIPEILKVKLEDIRKMEFTK----GKFWVYTKDGEVHSVP----IKDVAKFARNPCHH 257 Query: 261 CPDWWSGIADISI-SDGDPNIFDSSKEGISAKASSTVMVRTKTGARLLELA 310 C D+ S ADIS+ S G P+ ++S V +RT+ G + ++ Sbjct: 258 CCDYTSVFADISVGSVGAPDGWNS------------VFIRTEIGEKYFDMV 296 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,253,735 Number of extensions: 1183824 Number of successful extensions: 2050 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2024 Number of HSP's successfully gapped: 15 Length of query: 382 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 274 Effective length of database: 25,922,359 Effective search space: 7102726366 Effective search space used: 7102726366 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)