BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0036.p (733 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0910 [G] COG2730 Endoglucanase 60 1e-08 >CAC0910 [G] COG2730 Endoglucanase Length = 1483 Score = 60.5 bits (145), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 34/82 (41%), Positives = 54/82 (65%), Gaps = 3/82 (3%) Query: 14 DSIISPVSAAFDKKQTQQADVTIELTLNGNTLIGISNGIAN-LTAGTDYTLSGSTVTISK 72 +S+++ S +D+ Q D + +TLNGNT+ + + N L AG+DYT++ +T+S+ Sbjct: 1006 NSVVNTSSVTYDQNAPQ--DQAVSITLNGNTITDVKDASGNTLKAGSDYTVTSDGITLSQ 1063 Query: 73 TYLASLAAGTTSLTFRFSAGAA 94 +YLA+LAAGT + T FSAG A Sbjct: 1064 SYLATLAAGTYTYTVDFSAGNA 1085 Score = 58.5 bits (140), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 32/82 (39%), Positives = 46/82 (56%), Gaps = 2/82 (2%) Query: 11 PELDSIISPVSAAFDKKQTQQADVTIELTLNGNTLIGISNGIANLTAGTDYTLSGSTVTI 70 P +D +I+P +A FD DV + + LNG TL G+++ +YT+SGS + + Sbjct: 192 PVVDPVITPTTATFDTAN--PTDVNVAMQLNGFTLSGLTDENGTAVDAANYTISGSNLVL 249 Query: 71 SKTYLASLAAGTTSLTFRFSAG 92 SK YLA LA G S F F+ G Sbjct: 250 SKAYLAKLALGKHSFNFNFAKG 271 Score = 57.0 bits (136), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 33/81 (40%), Positives = 51/81 (62%), Gaps = 3/81 (3%) Query: 15 SIISPVSAAFDKKQTQQADVTIELTLNGNTLIGISNGIAN-LTAGTDYTLSGSTVTISKT 73 S+++ S +D+ Q D + +T NGNT+ + + N L AG+DYT + +T+S++ Sbjct: 764 SVVNTSSVTYDQNAPQ--DQAVSITFNGNTVKDVKDASGNTLKAGSDYTATSDGITLSQS 821 Query: 74 YLASLAAGTTSLTFRFSAGAA 94 YLA+LAAGT + T FSAG A Sbjct: 822 YLATLAAGTYTYTIDFSAGNA 842 Score = 55.5 bits (132), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 34/83 (40%), Positives = 54/83 (65%), Gaps = 3/83 (3%) Query: 13 LDSIISPVSAAFDKKQTQQADVTIELTLNGNTLIGISNGIAN-LTAGTDYTLSGSTVTIS 71 ++S ++ S +D Q D + +TLNGNT+ + + A+ L AG+DYT++ +T+S Sbjct: 1248 VNSSVNTSSVTYDPAAPQ--DQAVSVTLNGNTITDVKDANASVLKAGSDYTVTSDGITLS 1305 Query: 72 KTYLASLAAGTTSLTFRFSAGAA 94 ++YLA+LAAGT + T FSAG A Sbjct: 1306 QSYLATLAAGTYTYTVDFSAGNA 1328 Score = 50.8 bits (120), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 32/75 (42%), Positives = 43/75 (57%), Gaps = 3/75 (4%) Query: 17 ISPVSAAFDKKQTQQADVTIELTLNGNTLIGISNGIANLTAGTDYTLSGS-TVTISKTYL 75 + P S FDK Q D + L LNG+TL + NL GTDYT++ TVT SK YL Sbjct: 291 VDPASVVFDKVAPQ--DENVALKLNGHTLGDVVGPKGNLVKGTDYTVADDGTVTFSKAYL 348 Query: 76 ASLAAGTTSLTFRFS 90 ++L G ++TF+ S Sbjct: 349 STLPLGDQTITFKAS 363 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.311 0.127 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 47,837,927 Number of extensions: 1909717 Number of successful extensions: 3917 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 3911 Number of HSP's successfully gapped: 7 Length of query: 733 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 619 Effective length of database: 25,230,355 Effective search space: 15617589745 Effective search space used: 15617589745 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 120 (50.8 bits)