BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0030.p (194 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L152977 [G] COG0406 Fructose-2,6-bisphosphatase 79 5e-15 RSc0499 [G] COG0406 Fructose-2,6-bisphosphatase 78 7e-15 BH0285 [G] COG0406 Fructose-2,6-bisphosphatase 68 1e-11 Cgl1034 [G] COG0406 Fructose-2,6-bisphosphatase 61 8e-10 DR1393 [G] COG0406 Fructose-2,6-bisphosphatase 57 1e-08 >L152977 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 78.6 bits (192), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 13/185 (7%), Positives = 36/185 (19%), Gaps = 9/185 (4%) Query: 5 TYIYMVRHGDSLKTSGNERSRFLSAQGEKDAQRVTQRLLGEGIDALYSSPYTRAVDTIAG 64 Y + + T G F E DA + +GI+ + + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 65 LAGALGKEIHKVEDLKEKCWTEDDRTLQDPELYPFLEQMFAEPDFVLEDGGESNRVCKER 124 + + + ++ + ++ N Sbjct: 62 QGLTETYVGNWLAKHGSR-----EKLIIASKVSGPSRNNDKGIRPDQAL-DRKNIREALH 115 Query: 125 AVKALKEILRNHPGERIVIGTHGMVMTLMMNEFAEEYGL-DFL-LQTRKPDIYRMEMKDG 182 + + + + + L + R Sbjct: 116 DSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRY 175 Query: 183 -LLSH 186 +S+ Sbjct: 176 IGVSN 180 >RSc0499 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 78.2 bits (191), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 5/164 (3%), Positives = 23/164 (14%), Gaps = 14/164 (8%) Query: 1 MTTTTYIYMVRHGDSLKTSGNERSRF-------LSAQGEKDAQRVTQRLLGEGIDALYSS 53 + +T +E D + L + Sbjct: 11 LEVSTLGLGTMTFG---EQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 54 PYTRAVDTIAGLAGALGKEIHKVEDLKEKCWT--EDDRTLQDPELYP--FLEQMFAEPDF 109 + + + ++ L+++ + Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 110 VLEDGGESNRVCKERAVKALKEILRNHPGERIVIGTHGMVMTLM 153 + + + + Sbjct: 128 LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAG 171 >BH0285 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 67.8 bits (164), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 9/178 (5%), Positives = 32/178 (17%), Gaps = 13/178 (7%) Query: 7 IYMVRHGDSLKTSGNERSRFLSAQGEKDAQRVTQRLLGEGIDALYSSPYTRAVDTIAGLA 66 + ++ + +L + Sbjct: 4 HRIPHSSLE---VSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 67 GALGKEIHKVEDLKEKCWTEDDRTLQDPELYPFLEQMFAE-PDFVLE-DGGESNRVCKER 124 E + L + ++ + + D + D + +E Sbjct: 61 TQGLTETYVGNWLAK-------HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREA 113 Query: 125 AVKALKEILRNHPGERIVIGTHGMVMTLMMNEFAEEYGLDFLLQTRKPDIYRMEMKDG 182 +LK + ++ + + + E + Sbjct: 114 LHDSLKRLQTDY-LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRA 170 >Cgl1034 [G] COG0406 Fructose-2,6-bisphosphatase Length = 233 Score = 61.2 bits (147), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 51/164 (31%), Positives = 75/164 (45%), Gaps = 11/164 (6%) Query: 2 TTTTYIYMVRHGDSLKTS----GNERSRFLSAQGEKDAQRVTQRLLGEGIDALYSSPYTR 57 T +T + ++RHG + T G LS +GE+ A+ V QRL I A+YSSP R Sbjct: 16 TQSTVVLLIRHGQTPTTGQVLPGQTPGLHLSDKGEEQAREVAQRLAEVPITAVYSSPMER 75 Query: 58 AVDTIAGLAGALGKEIHKVEDLKEKC----WTEDDRTLQDPELYPFLEQMFAEPDFVLED 113 A +T A A G E+ VE +C WT R L + + + P Sbjct: 76 AQETAAPTVSAHGLEL-TVEPGLIECDFGEWT--GRKLTELNALEEWKAVQKTPSTFRFP 132 Query: 114 GGESNRVCKERAVKALKEILRNHPGERIVIGTHGMVMTLMMNEF 157 GGES ++R V+A+ I + HPGE + +H + + F Sbjct: 133 GGESFVEMQDRMVEAIGNIAQQHPGEIVAAFSHADTIKAAVAHF 176 >DR1393 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 57.4 bits (137), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 6/168 (3%), Positives = 22/168 (13%), Gaps = 21/168 (12%) Query: 3 TTTYIYMVRHGDSLKTSGN----ERSRFLSAQGEKDAQRVTQRLLGEGIDALYSSPYTRA 58 + + + + + + Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKH 76 Query: 59 VDTIAG----LAGA----LGKEIHKVE--DLKEKCWTEDDRTLQDPELYP-FLEQMFAEP 107 K I + D K D L + + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHD------SLKRLQTDYLDLYQ 130 Query: 108 DFVLEDGGESNRVCKERAVKALKEILRNHPGERIVIGTHGMVMTLMMN 155 + + + + + + + Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,471,917 Number of extensions: 584180 Number of successful extensions: 1252 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 1252 Number of HSP's successfully gapped: 9 Length of query: 194 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 93 Effective length of database: 26,729,697 Effective search space: 2485861821 Effective search space used: 2485861821 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)