BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0029.p (426 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value slr0309 [C] COG1032 Fe-S oxidoreductase 245 8e-65 TM1537 [C] COG1032 Fe-S oxidoreductase 152 1e-36 STM1537 [C] COG1969 Ni,Fe-hydrogenase I cytochrome b subunit 152 1e-36 alr2104 [C] COG1032 Fe-S oxidoreductase 147 2e-35 MK0895 [C] COG1032 Fe-S oxidoreductase 134 2e-31 >slr0309 [C] COG1032 Fe-S oxidoreductase Length = 473 Score = 245 bits (626), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 145/434 (33%), Positives = 231/434 (53%), Gaps = 22/434 (5%) Query: 1 MKKSILLIQPENQKINSFRRKQFNNFVQ-ITMPYLAGFIDEAKYKITLVDEYQQQIPYTR 59 MK I+++ I +R+ +FV +T +LA + AK+ + ++ + + + Sbjct: 13 MKIDIIIVY-----IQRYRKGHEVHFVPPLTGIHLAA-LTPAKHTVRVIHQQVETVNLDS 66 Query: 60 KFDLVAITVNTPNSGHCYKMAEKFQAAGAKVVMGGPHATLLPAEVSEHCDIIVIGEAEAT 119 + D++ ++ + + Y++A +++ G + GGPH T P E D IVIGE E+ Sbjct: 67 EADVIVLSFFSGFAPEAYRLATEYRKRGKITIAGGPHVTFAPEEALRFVDSIVIGEGESV 126 Query: 120 WPQLLEDFYQGKYQTEYRSDAIPELTNLPLPRWDLLHRNPLMKGAVISTRGCPYNCSYCN 179 W Q+ +D Q Q Y +AIP LTN+P PR+DLL ++ V +TRGCP+ CS+C Sbjct: 127 WEQIFQDIEQNTLQQRYIGEAIP-LTNIPTPRYDLLPPQYFIRRVVQATRGCPFTCSFCT 185 Query: 180 LKQIYHNRFRTRPVSEVIHEISLMKSRFFV------FWDDNFFADKAHAIEVMEQLRPLR 233 + + + FRTRPV V+++I K +++ FWDDN A++ + E++ Q+ PLR Sbjct: 186 VP-VLNPGFRTRPVEAVLNDIRYNKFKWWWQRKTVWFWDDNLTANRPYIKELLRQMIPLR 244 Query: 234 KKWAAQVTLADCNNDELLEKASAAGCLYLFVGLESFSPAALRGVNKGMNRVDAYRDIIGK 293 K W Q ++ +DELL+ +GC+ +F G+ESF +L K N+V Y+ I K Sbjct: 245 KWWLTQASMDITKDDELLDLMRDSGCIGIFFGIESFGQESLEDAGKRQNKVAEYQGKIKK 304 Query: 294 LHRHNIMIQAGIVFGFDEDTPDSFRQTLEGCEALGIDGVTVSLLTPLPRTPIYARMKREQ 353 LH I + AG + G D DTP+S R E +G+D +S+LTP T Y++M Sbjct: 305 LHDRGIGVMAGFISGLDGDTPESIRDMANQLEEIGVDVPFLSILTPFKGTRSYSKMMDTD 364 Query: 354 RLLHE-DWSLYNGKTDVVFHPAGMTSEQLYEGYLDFRRRFYSLPSFIRRMRVS-----GT 407 RL + W YNG +V F P+ MT +L + + D R +SL + RR+ S G Sbjct: 365 RLRSDLGWEFYNG-YNVTFVPSKMTPAELLKAHRDLWRSAFSLQAIFRRIGRSLFTLRGG 423 Query: 408 HPVYNFVMNLGYRL 421 + +MNL Y L Sbjct: 424 AMMMCSIMNLFYGL 437 >TM1537 [C] COG1032 Fe-S oxidoreductase Length = 346 Score = 152 bits (383), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 0/405 (0%), Positives = 0/405 (0%), Gaps = 37/405 (9%) Query: 29 ITMPYLAGFIDEAKYKITLVDEYQQQIPYTR----KFDLVAITVNTPNSGHCYKMAEKFQ 84 A + Y + N + EK Sbjct: 24 GEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLI 83 Query: 85 AAGAKVVMGGPHATLLPAEVSEH--CDIIVIGEAEATWPQLLEDFYQGK---------YQ 133 A + + + + + ++ D YQ + Sbjct: 84 IASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGK 143 Query: 134 TEYRSDAIPE-----LTNLPLPRWDLLH--RNPLM---KGAVISTRGCPYNCSYCNLKQI 183 Y T L + R + + + Sbjct: 144 LGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVT 203 Query: 184 YHNRFRTRPVSEVIHEISLMKSRFF---VFWDDNFFADKAHAIEVMEQL--RPLRKKWAA 238 N + S + + + + F + + R Sbjct: 204 IQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFT 263 Query: 239 QVTLAD--CNNDELLEKASAAGCLYLFVGLESFSPAALRGVNKGMNRVDAYRDIIGKLHR 296 + + L +F + + + + Sbjct: 264 -RYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLL-GATTMDQLKTNIES 321 Query: 297 HNIMIQAGIVFGFDEDTPDSFRQTLEGCEALGIDGVTVSLLTPLPRTPIYARMKREQRLL 356 Sbjct: 322 -- 379 Query: 357 HEDWSLYNGKTDVVFHPAGMTSEQLYEGYLDFRRRFYSLPSFIRR 401 Sbjct: 380 - 423 >STM1537 [C] COG1969 Ni,Fe-hydrogenase I cytochrome b subunit Length = 247 Score = 152 bits (383), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 0/405 (0%), Positives = 0/405 (0%), Gaps = 37/405 (9%) Query: 29 ITMPYLAGFIDEAKYKITLVDEYQQQIPYTR----KFDLVAITVNTPNSGHCYKMAEKFQ 84 + + + + + + + + + + Sbjct: 24 MHLKEITSQGYYIYEAPVRLWHWITALSIVVLAVTGYFIGRPLPSIQGEATFMFWMGWIR 83 Query: 85 AAGAKVVMGGPHATLLPAEVSEH--CDIIVIGEAEATWPQLLEDFYQGK---------YQ 133 A L + + + Sbjct: 84 LIHFTTAYIFTVALLFRIYWACVGNEYAREMFLVPFWRRAWRKGVISEIRWYFFLEKEAH 143 Query: 134 TEYRSDAIPE-----LTNLPLPRWDLLH--RNPLM---KGAVISTRGCPYNCSYCNLKQI 183 Y + + + + + A +L Sbjct: 144 RYYGHNPVAGLAVMFYFWMSVLMVCSGFALYGEGLGTDSWAYQWFGWMIRLTGNDSLALH 203 Query: 184 YHNRFRTRPVSEVIHEISLMKSRFF---VFWDDNFFADKAHAIEVMEQL--RPLRKKWAA 238 + +R + + R Sbjct: 204 FWHRLGMWFIIAFVIAHVYTAIREDIMS 263 Query: 239 QVTLAD--CNNDELLEKASAAGCLYLFVGLESFSPAALRGVNKGMNRVDAYRDIIGKLHR 296 Sbjct: 264 -- 321 Query: 297 HNIMIQAGIVFGFDEDTPDSFRQTLEGCEALGIDGVTVSLLTPLPRTPIYARMKREQRLL 356 Sbjct: 322 -- 379 Query: 357 HEDWSLYNGKTDVVFHPAGMTSEQLYEGYLDFRRRFYSLPSFIRR 401 Sbjct: 380 - 423 >alr2104 [C] COG1032 Fe-S oxidoreductase Length = 346 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 0/310 (0%), Positives = 0/310 (0%), Gaps = 17/310 (5%) Query: 62 DLVAITVNTPNSGHCYKMAEKFQAAGAKVVMGGPHATLLPAE-VSEHCDIIVIGEAEATW 120 V + S +A K G L + + E + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 121 PQLLEDFYQGKYQTEYRSDAIPELTNLPLPRWDLLHRNPLMKGAVISTRGCPYNCSYCNL 180 + Q + + + D L + A N + + Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 181 KQIYHNRFRTRPVSEVIHEISLM-----KSRFFVFWDDNFFADKAHAIEVMEQLRPLRKK 235 + H + V + + + A + K Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAE-VSQYEGVELLAYSCLGFGTLTGKY 245 Query: 236 --------WAAQVTLADCNNDELLEKASAAGCLYLFVGLESFSPAALRGVNKGMNRVDAY 287 + E+ A Y+ + A + + Sbjct: 246 LNGAKPAGARNTLFSRF--TRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 288 RDIIGKLHRHNIMIQAGIVFGFDEDTPDSFRQTLEGCEALGIDGVTVSLLTPLPRTPIYA 347 Sbjct: 304 363 Query: 348 RMKREQRLLH 357 Sbjct: 364 373 >MK0895 [C] COG1032 Fe-S oxidoreductase Length = 346 Score = 134 bits (338), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 0/427 (0%), Positives = 0/427 (0%), Gaps = 51/427 (11%) Query: 5 ILLIQPENQKINSFRRKQFNNFVQITMPYLAGFIDEAKYKITLVD------EYQQQIPYT 58 I + ++ Y Sbjct: 3 YHRIPHSSL--EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 59 RKFDLVAITVNTPNSGHCYKMAEKFQAAGAKVVMGGPHATLLPAEV--SEHCDIIVIGEA 116 + N + A + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 117 EATWPQLLE----------------DFYQGKYQTEYRSDAIPE-LTNLPLPRWDLLHRNP 159 T L + D + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 160 LMKGA-----VISTRGCPYNCSYCNLKQIYHNRFRTRPVSEVIHEISLMKSRFFV----F 210 ++ + NR ++EV + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 211 WDDNFFADKAHAIEVMEQLRP--LRKKWAAQVTLADCNNDELLEKASAAGCLYLFVGLES 268 + A Q D + L Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKA-VAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 269 FSPAALRGVNKGMNRVD--AYRDIIGKLHRHNIMIQAGIVFGFD-EDTPDSFRQTLEGCE 325 A+ M+++ + ++ Sbjct: 300 PFVASTLLGATTMDQLKTNIESLHL---ELSEDVLAEIEAVHQVY- 355 Query: 326 ALGIDGVTVSLLTPLPRTPIYARMKREQRLLHEDWSLYNGKTDVVFHPAGMTSEQ----L 381 Sbjct: 356 - 414 Query: 382 YEGYLDF 388 Sbjct: 415 421 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.139 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,523,393 Number of extensions: 1370625 Number of successful extensions: 2360 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 2313 Number of HSP's successfully gapped: 27 Length of query: 426 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 317 Effective length of database: 25,807,025 Effective search space: 8180826925 Effective search space used: 8180826925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 117 (49.7 bits)