BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0025.p (223 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1828 [R] COG4122 Predicted O-methyltransferase 291 6e-79 mlr0279 [R] COG4122 Predicted O-methyltransferase 236 2e-62 Rv0187 [R] COG4122 Predicted O-methyltransferase 181 1e-45 MT0197 [R] COG4122 Predicted O-methyltransferase 181 1e-45 PA1200 [R] COG4122 Predicted O-methyltransferase 108 8e-24 >BH1828 [R] COG4122 Predicted O-methyltransferase Length = 223 Score = 291 bits (745), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 137/222 (61%), Positives = 171/222 (77%) Query: 1 MGEMNTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAA 60 M E TW +D YF+ +L DPV+D VL+AN A LP IDVAPNQGK LYLLAK+KG Sbjct: 1 MEERETWEALDQYFSEKLHAFDPVMDWVLEANEKAELPPIDVAPNQGKFLYLLAKLKGVK 60 Query: 61 NILEIGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPAL 120 ILEIGTLGGYS+IW+ARALP+ GRLV+LE+ KH +A N++ AG+ K ++I GPAL Sbjct: 61 TILEIGTLGGYSSIWMARALPDDGRLVTLEFSEKHAAIAQSNMKKAGVDGKIDVIIGPAL 120 Query: 121 ESLAALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSK 180 E+L L+ +G + FD IFIDADKPNNPHYL+WALKL+ PG +IV DNVVR G V+ +S Sbjct: 121 ETLPTLKDKGFSSFDMIFIDADKPNNPHYLEWALKLSTPGTLIVGDNVVRNGNVLNKESA 180 Query: 181 DDRVQGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVIS 222 D ++GIRQFI LL+ EPR+E+TA+QTVG KGYDGFV+GV+ Sbjct: 181 DPSIKGIRQFIHLLSEEPRIESTAIQTVGIKGYDGFVVGVVK 222 >mlr0279 [R] COG4122 Predicted O-methyltransferase Length = 301 Score = 236 bits (601), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 18/219 (8%), Positives = 37/219 (16%) Query: 3 EMNTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAANI 62 ++ R L L + + A + Sbjct: 2 SIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYV 61 Query: 63 LEIGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPALES 122 G A + VV + + Sbjct: 62 DTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVIL 121 Query: 123 LAALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSKDD 182 D + + ++L A G + + + +A Sbjct: 122 AVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFP 181 Query: 183 RVQGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVI 221 + +A+E E Y V Sbjct: 182 EDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIER 220 >Rv0187 [R] COG4122 Predicted O-methyltransferase Length = 220 Score = 181 bits (458), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 105/213 (49%), Positives = 142/213 (66%), Gaps = 4/213 (1%) Query: 9 EVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAANILEIGTL 68 +VD + +S L+ DP L L A+ A LP I V+ QGK L LLA A +LEIGTL Sbjct: 11 DVDAFLDSTLVGDDPALAAALAASDAAELPRIAVSAQQGKFLCLLAGAIQARRVLEIGTL 70 Query: 69 GGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPALESLAALEA 128 GG+STIWLAR GR+V+LEY+ KH VA NL+ AG+AD+ E++ GPAL++L L Sbjct: 71 GGFSTIWLARGAGPQGRVVTLEYQPKHAEVARVNLQRAGVADRVEVVVGPALDTLPTL-- 128 Query: 129 RGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSKDDRVQGIR 188 PFD +FIDADK NN Y++WA++LA GAVIV DNV+RGG ++ A+S D R Sbjct: 129 -AGGPFDLVFIDADKENNVAYIQWAIRLARRGAVIVVDNVIRGGGIL-AESDDADAVAAR 186 Query: 189 QFIDLLAAEPRVEATALQTVGSKGYDGFVLGVI 221 + + ++ P ++ATA+QTVG KG+DGF L ++ Sbjct: 187 RTLQMMGEHPGLDATAIQTVGRKGWDGFALALV 219 >MT0197 [R] COG4122 Predicted O-methyltransferase Length = 218 Score = 181 bits (458), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 105/213 (49%), Positives = 142/213 (66%), Gaps = 4/213 (1%) Query: 9 EVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAANILEIGTL 68 +VD + +S L+ DP L L A+ A LP I V+ QGK L LLA A +LEIGTL Sbjct: 9 DVDAFLDSTLVGDDPALAAALAASDAAELPRIAVSAQQGKFLCLLAGAIQARRVLEIGTL 68 Query: 69 GGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPALESLAALEA 128 GG+STIWLAR GR+V+LEY+ KH VA NL+ AG+AD+ E++ GPAL++L L Sbjct: 69 GGFSTIWLARGAGPQGRVVTLEYQPKHAEVARVNLQRAGVADRVEVVVGPALDTLPTL-- 126 Query: 129 RGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSKDDRVQGIR 188 PFD +FIDADK NN Y++WA++LA GAVIV DNV+RGG ++ A+S D R Sbjct: 127 -AGGPFDLVFIDADKENNVAYIQWAIRLARRGAVIVVDNVIRGGGIL-AESDDADAVAAR 184 Query: 189 QFIDLLAAEPRVEATALQTVGSKGYDGFVLGVI 221 + + ++ P ++ATA+QTVG KG+DGF L ++ Sbjct: 185 RTLQMMGEHPGLDATAIQTVGRKGWDGFALALV 217 >PA1200 [R] COG4122 Predicted O-methyltransferase Length = 220 Score = 108 bits (269), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 66/165 (40%), Positives = 93/165 (56%), Gaps = 1/165 (0%) Query: 42 VAPNQGKLLYLLAKMKGAANILEIGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALD 101 +AP QG+ + LL + GA LE+GT GYS + +A AL E GRL + + + A Sbjct: 44 IAPEQGQFIALLLTLIGARRALEVGTFTGYSALCMATALGEQGRLTCCDLPGDYHLTARR 103 Query: 102 NLRLAGLADKTEIIEGPALESLAALEARG-AAPFDFIFIDADKPNNPHYLKWALKLACPG 160 R A + ++ E+ GPALE+L AL G A FD FIDADK N P YL+ AL L G Sbjct: 104 YWREAAVEERIELRLGPALETLRALLDEGLAGQFDLAFIDADKANYPEYLEAALALVRQG 163 Query: 161 AVIVADNVVRGGEVVQADSKDDRVQGIRQFIDLLAAEPRVEATAL 205 +++ DNV+ G V++A K +GI+Q L + RV+ + L Sbjct: 164 GLVLFDNVLWSGRVLEAQPKSADTRGIQQLNLALKNDARVDYSLL 208 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,383,283 Number of extensions: 755709 Number of successful extensions: 2409 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2403 Number of HSP's successfully gapped: 12 Length of query: 223 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 121 Effective length of database: 26,614,363 Effective search space: 3220337923 Effective search space used: 3220337923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)