BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0020.p (416 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1301 [GEPR] COG0477 Permeases of the major facilitator superf... 222 8e-58 b2681m [GEPR] COG0477 Permeases of the major facilitator superfa... 162 1e-39 CAC0560 [GEPR] COG0477 Permeases of the major facilitator superf... 157 4e-38 >SMa1301 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 385 Score = 222 bits (566), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 162/377 (42%), Positives = 203/377 (53%), Gaps = 1/377 (0%) Query: 39 LAASCGLIVANLYYAQTLVGPISAATHLSSGAAGLIVTLTQIGYVIGLLFVVPLSDIIEN 98 +AA+CGLI ANLYY Q L GPI+ L + A GLIVTLTQIGY +GLL +VPL D++EN Sbjct: 1 MAAACGLIAANLYYTQPLAGPIAVDIGLPAEATGLIVTLTQIGYGLGLLLLVPLGDLVEN 60 Query: 99 RRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGLGSVAAQILVPYASYLAAEEHRGRV 158 RR IG+GSVA Q++VP+A+ LA + RGRV Sbjct: 61 RRLIVTMIGVVTLALIAAGLSTTPGPFLTASLAIGVGSVAVQMIVPFAANLAPDAARGRV 120 Query: 159 VGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAISAVIIALLTILLSRVLPKRQPIPTMN 218 VGN+MSGL++GIM ARP++ ++GL WHA+F ISA+++ L LL LP R P ++ Sbjct: 121 VGNIMSGLMVGIMMARPISGLIAGLSSWHAVFYISAIVMVGLGTLLWVQLPIRMPTARLS 180 Query: 219 YGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLFWTTVSLRLADT-YHLSQQXXXXXXX 277 YG L++S+ LL PVL+RRA YQA F FSLFWT L LA + L Sbjct: 181 YGQLLKSMAQLLAAQPVLQRRAAYQAFQFAAFSLFWTVTPLYLAGPRFGLGHNGIALFAL 240 Query: 278 XXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXXXXXXXXXQSDSKVTLGLLVVGAIIL 337 D+G RP T S + L LL + AI+L Sbjct: 241 AGVAGAIASPIAGRLADKGLVRPATAFGLLSVGVAFLVTQIASEGSAIALTLLTLAAILL 300 Query: 338 DMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFFIGGAIXXXXXXXXXXXXXXXXTTLI 397 D GV+ LV GQR+IY LG E R RLNGLFMAIFF GGAI ++I Sbjct: 301 DFGVTMTLVTGQRSIYELGAELRSRLNGLFMAIFFTGGAIGSALGAWAFASGGWWFASMI 360 Query: 398 GLALPVLAFLYYLTEKK 414 G ALP A +LTEK Sbjct: 361 GFALPATAVAIFLTEKH 377 >b2681m [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 392 Score = 162 bits (409), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 113/340 (33%), Positives = 169/340 (49%), Gaps = 7/340 (2%) Query: 38 LLAASCGLIVANLYYAQTLVGPISAATHLSSGAAGLIVTLTQIGYVIGLLFVVPLSDIIE 97 L++ + GL VA+ YYAQ L+ I+ LS+ +AG IVT Q+GY GLLF+VPL D+ E Sbjct: 15 LMSIATGLAVASNYYAQPLLDTIARNFSLSASSAGFIVTAAQLGYAAGLLFLVPLGDMFE 74 Query: 98 NRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGLGSVAAQILVPYASYLAAEEHRGR 157 RR GL SV AQILVP A+ LA+ + RG+ Sbjct: 75 RRRLIVSMTLLXXMLITASSQSLAMMILGTALT--GLFSVVAQILVPLAATLASPDKRGK 132 Query: 158 VVGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAISAVIIALLTILLSRVLPKRQPIPTM 217 VVG +MSGLLLGI+ AR +A ++ L GW +F +++V++AL+ + L R LP+ + + Sbjct: 133 VVGTIMSGLLLGILLARTVAGLLANLGGWRTVFWVASVLMALMALALWRGLPQMKSETHL 192 Query: 218 NYGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLFWTTVSLRL-ADTYHLSQQXXXXXX 276 NY L+ S+ ++ + +LR RAL F FS+ WT+++ L A ++ S Sbjct: 193 NYPQLLGSVFSMFISDKILRTRALLGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFG 252 Query: 277 XXXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXXXXXXXXXQSDSKVTLGLLVVGAII 336 D+G + T + L++G ++ Sbjct: 253 LAGAAGALGARPAGGFADKGKSHHTTTFGLLLLLLSWLAIWFGHTSVLA----LIIGILV 308 Query: 337 LDMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFFIGGA 376 LD+ V G + Q IY + +AR RL +M +FIGGA Sbjct: 309 LDLTVQGVHITNQTVIYRIHPDARNRLTAGYMTSYFIGGA 348 >CAC0560 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 441 Score = 157 bits (396), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 100/345 (28%), Positives = 174/345 (50%), Gaps = 5/345 (1%) Query: 34 WITFLLAASCGLIVANLYYAQTLVGPISAATHLSSGAAGLIVTLTQIGYVIGLLFVVPLS 93 +I F++A +C + VANLYY Q L+G I+ + +++ AG +VTLTQ+GY +G+LF+VPL Sbjct: 51 FIIFIMALTCTVTVANLYYCQPLLGQIAKSFNVTEANAGFVVTLTQVGYALGMLFLVPLG 110 Query: 94 DIIENRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGLGSVAAQILVPYASYLAAEE 153 DI E ++ IG ++ Q++VP+A++L+ E Sbjct: 111 DIKERKKLIIEMLLLVSVFLFAAFISRNYYVLLVTSFLIGFTTIVPQLIVPFAAHLSKPE 170 Query: 154 HRGRVVGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAISAVIIALLTILLSRVLPKRQP 213 RG+V+GNV+SGLL+GI+ +R + + + GW ++ I+++++ ++ + PK +P Sbjct: 171 ERGQVIGNVLSGLLIGILLSRVFSGIIGDILGWRNVYLIASLLMVSFLFIIVFLFPKSKP 230 Query: 214 IPTMNYGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLFWTTVSLRL-ADTYHLSQQXX 272 ++Y LI S+ L+K VLR ++ A +FG+FS+FWT + L + Y++ + Sbjct: 231 TSKISYKKLIFSMWGLIKREKVLREASINGALMFGSFSIFWTALVFLLESKVYNMGSKEA 290 Query: 273 XXXXXXXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXXXXXXXXXQSDSKVTLGLLVV 332 D+ R G + LV+ Sbjct: 291 GFFGLVGIVGALAAPVVGKVADKKTPRFAVGFAIIFSTIAYVCFWGMGYK----IYGLVI 346 Query: 333 GAIILDMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFFIGGAI 377 G I+LD+G V Q + ++ ++ R R+N +FM +FIGG+I Sbjct: 347 GVILLDLGNQTGQVSNQARVQAISDQERSRINTVFMVSYFIGGSI 391 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,746,480 Number of extensions: 799838 Number of successful extensions: 2726 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2721 Number of HSP's successfully gapped: 7 Length of query: 416 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 307 Effective length of database: 25,807,025 Effective search space: 7922756675 Effective search space used: 7922756675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)