BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0017.p (697 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein 171 3e-42 BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein 160 8e-39 BH1827 [NT] COG0840 Methyl-accepting chemotaxis protein 151 3e-36 BS_mcpA [NT] COG0840 Methyl-accepting chemotaxis protein 150 7e-36 BS_tlpB [NT] COG0840 Methyl-accepting chemotaxis protein 149 2e-35 >BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein Length = 662 Score = 171 bits (434), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 153/547 (27%), Positives = 272/547 (49%), Gaps = 37/547 (6%) Query: 166 REYFTEAFK--GKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKL 223 R ++ +A K G+ ++D V+ S+ +++ +Q +K DG GV+AM + D L+++ Sbjct: 138 RPWYQDAMKAGGEIVVTDPYVAASDGSMVITIAQELK--DGS--GVVAMDITIDKLLEQM 193 Query: 224 GNININAKGKIEVLSRGGIVLYNSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTELG 283 I + +G + ++ N + A G+ + + A D E+Q T L Sbjct: 194 KQIKVGKEGYAFIATK------NKTYVAHKNHKAGEKLSGDWVAKMYANDSGELQYT-LN 246 Query: 284 TEYLRI----NKIPKADLTISV-IDSYDDIERPIQDMLMKILIVTVIALLVAIGFGLLLS 338 E ++ N++ + ++ +D D + + M +LI +++A + I L + Sbjct: 247 NEDKKMTYTTNELTGWKIAGTMYMDEIKDASKSVLTTGMIVLIASIVAGGILI---LFIV 303 Query: 339 RSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALITNMNDSIT 398 RSITKP+ L KT++ GDLT T + K E +L +SFN M Q ++LI+ + +S+ Sbjct: 304 RSITKPLKRLVQSSKTISRGDLTETIEIHSKDELGELGESFNEMGQSLRSLISAIQNSVN 363 Query: 399 ILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFAGMND 458 + S+ +L ++ Q +++ I A+E SN E+ K S + MN+ Sbjct: 364 NVAASSEQLTASAGQTSKATEH-------ITMAIEQFSNGNEEQSRKVESSSHQLNLMNE 416 Query: 459 NAQAVRQRAEEITQVFHTSNQVV---EGLIE-----ISSKNEQEVQKISAITVKLQDSSN 510 Q V Q + +IT+ S ++ E ++ ++S N Q VQ+ A+ L+ S Sbjct: 417 GLQQVSQTSSDITKASIQSTEIAGTGEKFVQQTGGQMNSIN-QSVQQAEAVVKGLEGKSK 475 Query: 511 SISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYE 570 I++I IN IA QTNLLALNA+IEAARAGE GRGF+VVA E+RKLA QS+ + EI + Sbjct: 476 DITSILRVINGIADQTNLLALNAAIEAARAGESGRGFSVVAEEVRKLAVQSADSAKEIEK 535 Query: 571 IIQQNLAFVAENNDSVMEINQISAQQDELVGQTQTAFQTILDKITEITVQIQSMADEIAH 630 +IQ+ +A + + E+NQ + T+ +FQ+I EI ++Q+M + Sbjct: 536 LIQEIVAEIDTSLHMFKEVNQEVQSGLVVTDNTKESFQSIFSMTNEIAGKLQTMNSTVEQ 595 Query: 631 MQKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLAEA 690 + V + + EQ +++++++ T+ Q+++ L + Sbjct: 596 LSDRSQHVSAAVSGIADVSKESSASIQDIAASAEEQLASMEEISSSATTLAQMAEELRDL 655 Query: 691 ASKFRIE 697 +F+IE Sbjct: 656 TKQFKIE 662 >BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 83 Score = 160 bits (404), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 0/558 (0%), Positives = 0/558 (0%), Gaps = 55/558 (9%) Query: 164 VDREYFTEAF--KGKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLD 221 Sbjct: 135 ---- 190 Query: 222 KLGNININAKGKIEVLSRGGIVLYNSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTE 281 Sbjct: 191 ----------- 239 Query: 282 LGTEYLRINKIPKADLTISVIDSYD----DIERPIQDMLMKILIVTVIALLVAIGFGL-- 335 Sbjct: 240 -- 297 Query: 336 --LLSRSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALITNM 393 Sbjct: 298 357 Query: 394 NDSITILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSEKF 453 Sbjct: 358 ------- 410 Query: 454 AGMNDNAQAVRQRAEEITQVFHTSNQVVEGLIEISSKNEQEVQKISA------------- 500 Sbjct: 411 ------- 463 Query: 501 -ITVKLQDSSNSISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAE 559 Sbjct: 464 523 Query: 560 QSSRQSNEIYEIIQQNLAFVAENNDSVMEINQISAQQDELVGQTQTAFQTILDKITEITV 619 Sbjct: 524 583 Query: 620 QIQSMADEIAHMQKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGT 679 Sbjct: 584 643 Query: 680 IDQLSKSLAEAASKFRIE 697 Sbjct: 644 661 >BH1827 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 678 Score = 151 bits (382), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 116/444 (26%), Positives = 213/444 (47%), Gaps = 7/444 (1%) Query: 199 VKSADGKVLGVLAMTVASDFFLDKLGNININAKGKIEVLSRGGIVLYNSSDPARIGQDVG 258 V + ++LGV+A+ + D L + G + ++ G+V+ + R G+++ Sbjct: 174 VDPRNQQLLGVVAIDILLDDLSQLLNETEVGMGGSLALIHESGMVIAHPDADLR-GENLS 232 Query: 259 DDPGIKPLLETRATD---ELEIQTTELGTEYLRINKIPKADLTISVIDSYDDIERPIQDM 315 D+ I+ L + + E E + L NK+ + D I ++ P++ + Sbjct: 233 DESFIQDLQQAQGKQGQIEYEFENE---ARTLFFNKMDQMDWNIVAFYRDKEVTAPVRQL 289 Query: 316 LMKILIVTVIALLVAIGFGLLLSRSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDL 375 ++V ++++ +A R + P++ L R + + GDLT +AD + E L Sbjct: 290 RNYFILVLLVSMTIATATSYYFGRKMANPILALNDRMQQVIKGDLTASADVKSQDEIGQL 349 Query: 376 ADSFNTMVQQNKALITNMNDSITILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQ 435 +D FN MVQ + +I + + EL ++A S E + +S +A Q Sbjct: 350 SDGFNQMVQTLREMIHALRQTGATSNKEAVELRSMLQEAIASSEEVATSSTAVANGAVDQ 409 Query: 436 SNDTEQIVNKFYGFSEKFAGMNDNAQAVRQRAEEITQVFHTSNQVVEGLIEISSKNEQEV 495 + + E + S + M+ A + + A V + + VE L E + ++E+ + Sbjct: 410 AEEIEHAKSDIEKLSAQIDWMDQTASQINELAASTANVSASGLEQVETLREKTEEHERII 469 Query: 496 QKISAITVKLQDSSNSISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIR 555 QK+ I + I +I I + QTNLLALNASIEAARAGE+G+GF+VVA EIR Sbjct: 470 QKMEGIVNHFVHQTEEILSIVELIQSFSEQTNLLALNASIEAARAGEYGKGFSVVAEEIR 529 Query: 556 KLAEQSSRQSNEIYEIIQQNLAFVAENNDSVMEINQISAQQDELVGQTQTAFQTILDKIT 615 KLAEQS++ +N I +Q+ A + + + SA+Q + V Q+ +F I I Sbjct: 530 KLAEQSTKATNNIQSAVQKIKESGAHATEEMTKTAAQSAEQVKRVDQSIASFTHISSNIE 589 Query: 616 EITVQIQSMADEIAHMQKDKDEVL 639 ++ I+++ ++ M + +++ + Sbjct: 590 QVVNSIKTVVKQLQTMNESRNQAV 613 >BS_mcpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 661 Score = 150 bits (379), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 143/546 (26%), Positives = 269/546 (49%), Gaps = 23/546 (4%) Query: 160 GQSRVDREYFTEAF--KGKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASD 217 G + V+R+++ +A KGK I+D + S N +++ +Q K DG GV+A+ + + Sbjct: 131 GYNPVERDWYKKAVANKGKVVITDPYKTASTNTMVVTIAQQTK--DGS--GVIAINMTIE 186 Query: 218 FFLDKLGNININAKGKIEVLSRGGIVLYNSSDPARIGQDVGDDPGIKPLLETRATDELEI 277 L +NI +G ++++ V+ + ++ + G ++ D K L + D+ + Sbjct: 187 NLLKTTKKVNIGTQGYAFIMTKDKKVVAHPNEQS--GTELKGDWLDKML----SADKGDF 240 Query: 278 QTTELGTEY---LRINKIPKADLTISVIDSYDDIERPIQDMLMKILIVTVIALLVAIGFG 334 Q T G + NK+ + ++ D+I Q +L LIV A+++ I Sbjct: 241 QYTMDGDKKKMAFDTNKLTGWKIGGTMY--LDEIHEAAQPVLHLALIVLAAAIIIGIIVM 298 Query: 335 LLLSRSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALITNMN 394 L+ RSIT P+ L K ++ GDLT T D K E +L SFN M ++LI + Sbjct: 299 TLIIRSITTPLKQLVGSSKRISEGDLTETIDIRSKDELGELGKSFNNMASSLRSLIHAIQ 358 Query: 395 DSITILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFA 454 DS+ + S+ EL ++ Q +++ ++ + E Q+ + E Y ++ Sbjct: 359 DSVDNVAASSEELTASAAQTSKATEHITLAIEQFSNGNEKQNENIETAAEHIYQMNDGLT 418 Query: 455 GMNDNAQAVRQRAEEITQVFHTSNQVVEGLIEISSKNEQEVQKISAITVKLQDSSNSISN 514 M ++ + + + T++ ++V + + ++ V++ + L+ S I+N Sbjct: 419 NMAQASEVITDSSVQSTEIASEGGKLVHQTVGQMNVIDKSVKEAEQVVRGLETKSKDITN 478 Query: 515 ITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYEIIQQ 574 I IN IA QTNLLALNA+IEAARAGE+GRGF+VVA E+RKLA QS+ + EI +I Sbjct: 479 ILRVINGIADQTNLLALNAAIEAARAGEYGRGFSVVAEEVRKLAVQSADSAKEIEGLI-- 536 Query: 575 NLAFVAENNDSV---MEINQISAQQDELVGQTQTAFQTILDKITEITVQIQSMADEIAHM 631 + V E N S+ +NQ ++ +T+ +F+ I + +I ++Q+M+ + + Sbjct: 537 -IEIVKEINTSLGMFQSVNQEVQTGLDITDKTEMSFKRISEMTNQIAGELQNMSATVQQL 595 Query: 632 QKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLAEAA 691 +EV +++ + EQ +++++++ T+ +++ L + Sbjct: 596 SASSEEVSGASEHIASISKESSAHIQDIAASAEEQLASMEEISSSAETLSSMAEELRDMT 655 Query: 692 SKFRIE 697 +F+IE Sbjct: 656 KRFKIE 661 >BS_tlpB [NT] COG0840 Methyl-accepting chemotaxis protein Length = 83 Score = 149 bits (375), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 0/609 (0%), Positives = 0/609 (0%), Gaps = 62/609 (10%) Query: 126 ANTILQDSLQGGNDSLL---------VLDSQGTIVAGTNKESL----------GQSRVDR 166 Sbjct: 78 137 Query: 167 EYFTEAF--KGKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKLG 224 Sbjct: 138 --------- 188 Query: 225 NININAKGKIEVLSRGGIVLYNSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTELGT 284 Sbjct: 189 --------- 239 Query: 285 -EYLRINKIPKADLTISVIDSYD----DIERPIQDMLMKILIVTVIALLVAI----GFGL 335 Sbjct: 240 299 Query: 336 LLSRSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALITNMND 395 Sbjct: 300 ------- 352 Query: 396 SITILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFAG 455 Sbjct: 353 412 Query: 456 MNDNAQAVRQRAEEITQVFHTSNQVV---EGLIEISSKN----EQEVQKISAITVKLQDS 508 Sbjct: 413 472 Query: 509 SNSISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEI 568 Sbjct: 473 532 Query: 569 YEIIQQNLAFVAENNDSVMEINQISAQQDELVGQTQTAFQTILDKITEITVQIQSMADEI 628 Sbjct: 533 592 Query: 629 AHMQKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLA 688 Sbjct: 593 652 Query: 689 EAASKFRIE 697 Sbjct: 653 661 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.127 0.330 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,999,243 Number of extensions: 1462356 Number of successful extensions: 7076 Number of sequences better than 1.0e-05: 804 Number of HSP's gapped: 4432 Number of HSP's successfully gapped: 817 Length of query: 697 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 584 Effective length of database: 25,345,689 Effective search space: 14801882376 Effective search space used: 14801882376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 119 (50.4 bits)