BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0010.p (426 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 357 2e-98 APE0035 [G] COG1653 ABC-type sugar transport system, periplasmic... 96 1e-19 PH1039 [G] COG1653 ABC-type sugar transport system, periplasmic ... 94 4e-19 SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic ... 93 8e-19 VCA1033 [G] COG1653 ABC-type sugar transport system, periplasmic... 85 2e-16 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 357 bits (916), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 180/419 (42%), Positives = 260/419 (62%), Gaps = 5/419 (1%) Query: 5 LLVVLTACSGNNSTPAANGNGDSKGKTTTLKFFHRWPNEPRNSYFNYIKEEFEKQNPGVK 64 L +++ A S ++ + + GK T LKFFHRWP EP SYF + +EFEK +P + Sbjct: 6 LFLIIAAVSMLTIAGCSSQSSSADGKVT-LKFFHRWPKEPEKSYFEEVVKEFEKDHPDID 64 Query: 65 IEMESVLNDNYKEKIKVLVSSGDIPDVFSSWSDSFAESLVSSGRVEDLTSMYDSDPDWAK 124 I++E+VLND+YK+KIKV++ + PD++ SWSD FA + + DL+S Y +D DW+ Sbjct: 65 IQIEAVLNDSYKDKIKVMLGTTSPPDIYFSWSDEFAFKFIRGNKALDLSSYYKNDTDWSS 124 Query: 125 TLVQNQLKSFTFDDKIYGVPFTMDGKVFFYNKKIFSKLGLEKPKTWDEFIALLDKLKASG 184 LVQ+Q+ FT+++K YGVP+ MD K FFYNK IF KL L+ PKTWDE I + KLK G Sbjct: 125 QLVQSQITPFTYENKQYGVPWQMDAKSFFYNKDIFQKLNLDPPKTWDELIDVSKKLKEHG 184 Query: 185 YDTPIIEGLQDSWAVGHYLGTMNQRMLSPEVLATDYAVGTGQFTDPGYLQVLKYWQKLSS 244 Y TPI G + +W + HY+GT+NQRM+ + DY TG+FTD GY++ L+ Q+L Sbjct: 185 Y-TPISFGTKATWTISHYIGTLNQRMVDEKTREKDYNAKTGEFTDEGYVKALEKLQELMP 243 Query: 245 YMGPNATSVNHEATRNMFIAGEVPIHYLQFAELGYLKDKPDLEFDYFNFPQFSEGKGNPE 304 Y + SV+HE R F +G+ + Y + AE+ ++ + F FP+ S KG+ E Sbjct: 244 YFNKHVNSVDHEYVRQQFKSGKSAMIYAETAEIKLVE---PVNLGMFPFPEISGQKGSSE 300 Query: 305 GLTGAPEGFMISKETANKELAEKFVKFIVSPENAAKFQQDVGSMTTVIGAATKENSPPGT 364 LTGAPEGFMIS T + + A +F++F+ S K +DVG + V G AT+EN+ Sbjct: 301 ALTGAPEGFMISSRTKHPKEAMEFLQFLTSKRMGEKLVKDVGKYSAVQGTATEENATAIQ 360 Query: 365 LEAIEVIQNAAETTPWLDNAVDASVADAFMRGGQAIAADIETPENVLKEVQTAAEKLQA 423 EA++ I +A PW D VD VADA++ G Q + TP+ V+K VQ AA++++A Sbjct: 361 REAVQHIVDAKSMVPWFDMDVDVEVADAYLTGVQQMLGGDMTPQQVMKAVQKAAKQVRA 419 >APE0035 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 452 Score = 95.5 bits (236), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 88/339 (25%), Positives = 151/339 (44%), Gaps = 30/339 (8%) Query: 32 TTLKFFHRWPNEPRNSYFNYIKEEFEKQNPGVKIEMESVLNDNYKEKIKVLVSSGDIPD- 90 TL W + YF E+F ++NP V I+ + + + V +G P Sbjct: 74 VTLTVIGPWSGK-EQEYFMTALEKFMEENPNVTIKYVPMRAEEVARTLSVQFEAGVTPAD 132 Query: 91 -VFSSWSDSFAESLVSSGRVEDLTSMYDSDPDWAKTLVQNQLKSFTFDDKIYGVPFTMDG 149 V + W+ E + G V ++T + + D V L + +++K++GVPFTM Sbjct: 133 VVITPWAWWIVE-MAQKGHVIEVTGLINED-----EYVGGILDNVKWNNKLWGVPFTMWL 186 Query: 150 KV-FFYNKKIFSKLGLEKPKTWDEFIALLDKLKA-SGYDTPIIEGLQDSWAVGHYLGTMN 207 K F+Y K F+K GL +P +W+EF+ LLD++K G PI+ G W + Sbjct: 187 KPGFWYKKSFFAKHGLSEPNSWEEFLQLLDQIKGIEGIKNPIVSGDSVGWPLSDVTEHFI 246 Query: 208 QRMLSPEVLATDYAVGTGQ--FTDPGYLQVLKYWQKLSSYM--GPNATSVNHEATRNMFI 263 PE+ Y + TG+ FTDP QV++ ++KL+ + G + + + + Sbjct: 247 IAFGGPEL---QYKLITGEVSFTDP---QVVEIFEKLAMLIEQGYFSEPIEWTSAVEKWW 300 Query: 264 AGEVPIHYLQFAELGYLKDKPDLEFDYFNFPQFSEGKGNPEGLTGAPEGFMISKETANKE 323 A E ++++ G ++D DL F F P G+ G + I K T N E Sbjct: 301 AEEYALYFMGTWITGMVEDPNDLAF--FPLP-------GARGVVGGTDYAFIPKYTENLE 351 Query: 324 LAEKFVKFIVSPENAAKFQQDVGSMTTVIGAATKENSPP 362 A+ ++++ + A G + T +G + + PP Sbjct: 352 AAKLLLQYLATEGQAVHVSTPAGKVPTWLGVSVDQLWPP 390 >PH1039 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 420 Score = 94.4 bits (233), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 101/425 (23%), Positives = 180/425 (42%), Gaps = 38/425 (8%) Query: 1 MLAGLLVVLTACSG----NNSTPAANGNGDSKGKTTTLKFFHRWPNEPRNSYFNYIKEEF 56 +L G L++ T SG +T + TLK W +++ +K F Sbjct: 12 LLVGALIIATVASGCIGGTTTTETPTVTKTKTQEVITLKVIGPWSGAELDAFMEVLKA-F 70 Query: 57 EKQNPGVKIEMESVLNDNYKEKIKVLVSSGDIP-DVFSSWSDSFAESLVSSGRVEDLTSM 115 EKQ+P +KIE ++ ++ + + S D P DV W AE + G + +L ++ Sbjct: 71 EKQHPNIKIEYKTYRAEDLSTILPLQFESHDTPADVIFMWGWFIAE-MGKKGHLMELNNI 129 Query: 116 YDSDPDWAKTLVQNQLKSFTFDDKIYGVPFTMDGKV-FFYNKKIFSKLGLEKPKTWDEFI 174 + + V L S T D KIYG PFT K F+Y K F K L+ PKTWD+F+ Sbjct: 130 INPE-----EYVPGILDSVTVDGKIYGAPFTAAAKPGFWYRKSFFEKHNLKPPKTWDDFV 184 Query: 175 ALLDKL-KASGYDTPIIEGLQDSWAVG----HYLGTMNQRMLSPEVLATDYAVGTGQFTD 229 ALL+++ K G PI+ G W + H++ T + L +++ G ++ D Sbjct: 185 ALLEEIKKIPGIKAPIVSGDSVGWPLSDVTEHFILTFGGKDLQLKLIK-----GEVKWED 239 Query: 230 PGYLQVLKYWQKLSSYM--GPNATSVNHEATRNMFIAGEVPIHYLQFAELGYLKDKPDLE 287 P ++ + +KL + G + + + +++ GE ++++ G + D DL Sbjct: 240 PQVRKIFE--EKLVPLLKAGYFSDPIEWTSAVDLWWKGEYALYFMGTWITGMVDDPNDLG 297 Query: 288 FDYFNFPQFSEGKGNPEGLTGAPEGFMISKETANKELAEKFVKFIVSPENAAKFQQDVGS 347 P + + AP+ M+ K T++ + A + KF+ + G Sbjct: 298 L--IPLPGV-------KAMVIAPDYLMVPKYTSHPKEALELAKFLATEGQRIHVGTKSGK 348 Query: 348 MTTVIGAATKENSPPGTLEAIEVIQNAAETTPWLDNAVDASVADAFMRGGQAIAADIETP 407 + T + + P + + I E+ P LD++V F + + + Sbjct: 349 LATWKKVSIDDYWAP--MRDVAKIVANVESAPDLDDSVGGEWQKVFWDQLKLLWVQPDRL 406 Query: 408 ENVLK 412 + VLK Sbjct: 407 DEVLK 411 >SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 419 Score = 93.2 bits (230), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 105/368 (28%), Positives = 166/368 (45%), Gaps = 55/368 (14%) Query: 20 AANGNGDSKGKTTTLKFFHRWPNEPRNSYFNYIKEEFEKQNPGVKIEMESVLNDNYKEKI 79 A + G S T T+++F++ + I +FEK+NP +K+++ +V N E + Sbjct: 22 ACSNYGKSADGTVTIEYFNQ--KKEMTKTLEEITRDFEKENPKIKVKVVNVPNAG--EVL 77 Query: 80 KVLVSSGDIPDVFSSWSDSFA-ESLVSSGRVEDLTSMYDSDPDWAKTLVQNQLKSFTFDD 138 K V +GD+PDV + + S + +G EDL S+ D+ K + + + ++ Sbjct: 78 KTRVLAGDVPDVVNIYPQSIELQEWAKAGVFEDL-----SNKDYLKRVKNGYAEKYAVNE 132 Query: 139 KIYGVPFTMDGKVFFYNKKIFSKLGLEKPKTWDEFIALLDKLKASGYDTPIIEGLQDSWA 198 K+Y VPFT + +YNK F +LGL+ P+TWDEF L+ + A G TP D+W Sbjct: 133 KVYNVPFTANAYGIYYNKDKFEELGLKVPETWDEFEQLVKDIVAKG-QTPFGIAGADAWT 191 Query: 199 VGHYLGTMNQRMLSPEVLATDYAVGTGQFTDPGYLQVLKYWQ----KLSSYMGPNATSV- 253 + Y NQ LA A G G+ + Q L+Y Q KLS + + V Sbjct: 192 LNGY----NQ-------LAFATATGGGKEAN----QYLRYSQPNAIKLSDPIMKDDIKVM 236 Query: 254 -----NHEATRN-----------MFIAGEVPIH-YLQFAELGYLKDKPDLEFDYFNFPQF 296 N +N F G+V + +A + KP+ + F P Sbjct: 237 DILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIP-- 294 Query: 297 SEGKGNPEGLT-GAPE-GFMISKETANKELAEKFVKFIVSPENAAKFQQDVGSMTTVIGA 354 GK + LT GA + + IS T + + A FV+++ PE K+ GS T + G Sbjct: 295 --GKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPEVMQKYYDVDGSPTAIEGV 352 Query: 355 A-TKENSP 361 E+SP Sbjct: 353 KQAGEDSP 360 >VCA1033 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 458 Score = 85.1 bits (209), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 82/309 (26%), Positives = 142/309 (45%), Gaps = 25/309 (8%) Query: 40 WPNEPRNSYFNYIKEEFEKQNPGVKIEMESVLNDNYKEKIKVLVSSGDIPDVFSS--WSD 97 W + + + I FE NPG+K++ V N NY + + +G D+ + + D Sbjct: 27 WRADDKALWEQKIIPAFEAANPGIKVKFNPVPNVNYTPTLWENLKAGKAGDLITCRPFDD 86 Query: 98 SFAESLVSSGRVEDLTSM--YDSDPDWAKTLVQNQLKSFTFDDKIYGVPFTMDGKVFFYN 155 S A L +G + ++T M ++ P +A+ Q + TF VP FFYN Sbjct: 87 SLA--LFKAGHLAEITEMAGMENFPSFAQAPWQTDSGAQTF-----CVPMASVIHGFFYN 139 Query: 156 KKIFSKLGLEKPKTWDEFIALLDKLKASGYDTPIIEGLQDSWAVGHYLGTMNQRMLSPEV 215 KKIF++LGL P+T ++F +LDK+KA G P+ +SW V LG N + P Sbjct: 140 KKIFNELGLSVPQTREQFFTVLDKVKADGRYIPLSMSGSESW-VASELGYQN---IGPNY 195 Query: 216 LATD---YAVGTGQ--FTDPGYLQVLKYWQKLSSYMGPNATSVNHEATRNMFIAGEVPIH 270 + A+ GQ D Y++V + + +Y+G + ++ + +F +G+ ++ Sbjct: 196 WKGEDGRLALINGQEHLDDSQYVKVFEELARWRAYLGEDGELRDYGTSNELFTSGKAALY 255 Query: 271 YLQFAELGYLKDKPDLEFDYFNFPQFSEGKGNPEGLTGAPE-GFMISKETANKELAEKFV 329 E+ DK D F P +G G T + G ++ + N + A F+ Sbjct: 256 VAGSWEIAPFTDKVD--FGVMRPPVAKQGDGC--FFTDHTDIGMGMNPASKNPQAAMAFL 311 Query: 330 KFIVSPENA 338 +++ +PE A Sbjct: 312 QWLTTPEFA 320 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,473,087 Number of extensions: 1368126 Number of successful extensions: 2788 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 2763 Number of HSP's successfully gapped: 56 Length of query: 426 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 317 Effective length of database: 25,807,025 Effective search space: 8180826925 Effective search space used: 8180826925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)