BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0005.p (328 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0572 [M] COG2222 Predicted phosphosugar isomerases 249 6e-66 STM3601 [M] COG2222 Predicted phosphosugar isomerases 224 2e-58 STM4540 [M] COG2222 Predicted phosphosugar isomerases 154 2e-37 SMc03139 [M] COG2222 Predicted phosphosugar isomerases 139 7e-33 yhfN [M] COG2222 Predicted phosphosugar isomerases 137 3e-32 >STM0572 [M] COG2222 Predicted phosphosugar isomerases Length = 328 Score = 249 bits (635), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 133/314 (42%), Positives = 183/314 (58%), Gaps = 2/314 (0%) Query: 16 VAQRKVSNFYFVACGGSLANVTPAQYILDREIEIPSAAY-SSNEFIHRSPKALGPSSVVI 74 + ++ + +FV CGGSL P +Y LD E + Y +SNEF+H +PKALG +SVVI Sbjct: 16 LGKQNIERVWFVGCGGSLTGFWPGKYFLDCEASKLAVGYITSNEFVHATPKALGKNSVVI 75 Query: 75 TCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPYGEALRGEET 134 S GNT ETVAA AREKGA TI L D+PL E +++ + Y +A Y E + + Sbjct: 76 LASQQGNTAETVAAARVAREKGAATIGLVYQPDTPLCEYSDYIIEYQWARYPETVDPAQQ 135 Query: 135 SMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTLDAAKAFGRNYKREKL 194 A L IL +Y +S+ V+ + + DA + F +K EK+ Sbjct: 136 KAAYSLWLALEILAQTEGYAQYDELVSAFGRFSDVVHGAQRQVQEDAQR-FAAEWKDEKV 194 Query: 195 IYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDFDVPFIVIKGLGET 254 +Y M SG ++ A+ +IC+L EMQWI+S++IHSGEYFHGPFEIT+ PFI+++ G T Sbjct: 195 VYMMGSGPSFGAAHQESICILLEMQWINSASIHSGEYFHGPFEITEPGTPFILLQSSGRT 254 Query: 255 RPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGVVLRQYAQHLSEETG 314 RPLDDRA F +Y K LIDA+ ++ +S DV YF L+ VL Y L+ Sbjct: 255 RPLDDRAIRFIERYQGKLQLIDADKLGIQDLSTDVGEYFCGLLHNCVLDVYNLALATARN 314 Query: 315 HALSVRRYMWKMEY 328 H L+ RRYMWK+EY Sbjct: 315 HPLTTRRYMWKVEY 328 >STM3601 [M] COG2222 Predicted phosphosugar isomerases Length = 325 Score = 224 bits (571), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 126/312 (40%), Positives = 180/312 (57%), Gaps = 11/312 (3%) Query: 21 VSNFYFVACGGSLANVTPAQYILDREIE-IPSAAYSSNEFIHRSPKALGPSSVVITCSHS 79 V + Y+VACGGS A PA+ L++E + + Y+S EFI+ P ALG ++VV+ SH Sbjct: 21 VKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFINNPPVALGENAVVVVASHK 80 Query: 80 GNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPYGEALRGEETSMAIL 139 GNTPET+ A AR+ GA I LT ++DSPL ++ Y + G+ + GE+T +L Sbjct: 81 GNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETYTFGD-GKDIAGEKTMKGLL 139 Query: 140 YRLVFGILQTLYPNEKYANAISSIENGLHGVYE---RNKALTLDAAKAFGRNYKREKLIY 196 ++ L E YA+ ++G+ + R + A+AF + YK +K+IY Sbjct: 140 -----SAVELLQQTEGYAH-YDDFQDGVSKINRIVWRACEQVAERAQAFAQEYKDDKVIY 193 Query: 197 TMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDFDVPFIVIKGLGETRP 256 T+ASG Y AY +IC+ EMQWIHS+ IHSGE+FHGPFEITD + PF G TR Sbjct: 194 TVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTPFFFQFSEGNTRA 253 Query: 257 LDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGVVLRQYAQHLSEETGHA 316 +D+RA +F KY + ++DA+ L I V YF + + V Y + L+E H Sbjct: 254 VDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPVYNRALAEARQHP 313 Query: 317 LSVRRYMWKMEY 328 L+ RRYMWK+EY Sbjct: 314 LTTRRYMWKVEY 325 >STM4540 [M] COG2222 Predicted phosphosugar isomerases Length = 343 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 94/323 (29%), Positives = 155/323 (47%), Gaps = 8/323 (2%) Query: 7 QQLEAAIQAVAQRKVSNFYFVACGGSLANVTPAQYILDREIEIPSAAYSSNEFIHRSPKA 66 Q+ E + Q S+ +F + GGSLA + +P + E IH+ K Sbjct: 28 QKAEQVADEIYQAGFSSLFFASVGGSLAPMMAINEFAKELTTLPVYVEQAAELIHKGNKR 87 Query: 67 LGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPYG 126 L SVVIT S SG+T E+VA + + +G + +T DSPL +AA + + + Sbjct: 88 LNKDSVVITLSKSGDTKESVAIAEWCKAQGIRVVAITKNADSPLAQAATWHIPMRHK--- 144 Query: 127 EALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTLDAAKAFG 186 G E +LY L F +L Y S +E + + + A Sbjct: 145 ---NGVEYEYMLLYWLFFRVLSRNNEFASYDRFASQLEILPANLLKAKQKFDPQADAIAS 201 Query: 187 RNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDFDVPFI 246 R + + +++ + + + Y F++C+L+EMQW + + S E+FHG E+ + DVP I Sbjct: 202 RYHNSDYMMWVGGAEM-WGEVYLFSMCILEEMQWKRTRPVSSAEFFHGALELLEKDVPLI 260 Query: 247 VIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGVVL-RQY 305 ++KG G+ R LD+R F K +D ++ID + + L+GI + R V +L + Sbjct: 261 LVKGEGKCRALDERVERFASKITDNLVVIDPKAYALDGIDDEFRWIMAPCVVSTLLVDRL 320 Query: 306 AQHLSEETGHALSVRRYMWKMEY 328 A H + TGH+L +RRY + +Y Sbjct: 321 AAHFEKYTGHSLDIRRYYRQFDY 343 >SMc03139 [M] COG2222 Predicted phosphosugar isomerases Length = 337 Score = 139 bits (349), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 93/331 (28%), Positives = 151/331 (45%), Gaps = 23/331 (6%) Query: 7 QQLEAAIQAVAQRKVSNFYFVACGGSLANVTPAQYILDREIEIPSAAYSSNEFIHRSPKA 66 Q+L+ + + ++ N +F+ GG+ + PA +L R P+ E Sbjct: 21 QRLDDVVVSCLEKGAENVFFLGTGGAAILMQPAAQLLQRRSRFPAFIDLPAELFITGSVN 80 Query: 67 LGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPYG 126 L S+V+ S SG T E+VA A+E GA + L ++PL + + + + + Sbjct: 81 LTEKSIVVIPSLSGTTKESVALLAKAKEAGATVLSLVGHEETPLGKGGD----HAFVNFA 136 Query: 127 EALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIEN--GLHGVYERNKALTL----- 179 E ++TS Y LQ+L+ IEN + G ER L L Sbjct: 137 E----DDTSCESFY------LQSLFIALSIMRHRGEIENYDQIAGELERLPGLLLEVKRG 186 Query: 180 --DAAKAFGRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFE 237 D A+ F R +G + A+ + +C+L+EMQWI + +H+ ++FHG E Sbjct: 187 YEDKAETFARVLAGSDYHIVTGAGNAWPEAFYYGMCILEEMQWIRTRPVHASDFFHGTLE 246 Query: 238 ITDFDVPFIVIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLV 297 + + V I+ KG RPL DR +F Y+D+ ++D F L GIS +VR + +V Sbjct: 247 LVEKGVSVILFKGEDALRPLADRVQNFAPNYTDRLTVLDTADFALPGISQEVRGLVSPIV 306 Query: 298 AGVVLRQYAQHLSEETGHALSVRRYMWKMEY 328 VL + + HL H L+ RRY ++ Y Sbjct: 307 LATVLERISAHLEVMRNHPLTTRRYYKRVAY 337 >yhfN [M] COG2222 Predicted phosphosugar isomerases Length = 347 Score = 137 bits (344), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 97/321 (30%), Positives = 144/321 (44%), Gaps = 20/321 (6%) Query: 6 VQQLEAAIQAVAQRKVSNFYFVACGGSLANVTPAQYILDREIEIPSAAYSSNEFIHRSPK 65 V + A ++ + +R + YFVACG L A+++ DR ++ A S EF +P Sbjct: 36 VPLVHAIVEEMVKRDIDRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPY 95 Query: 66 ALGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPY 125 L VI S G T E + A R GALT T DSP+ AAEF + Y Sbjct: 96 RLDDRCAVIGVSDYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDY----- 150 Query: 126 GEALRGEETSMAILYRLVFGILQTLYPNE---KYANAISSIENGLHGVYERNKALTLDAA 182 +A E + + Y +V ++ L PN K N + + N L + + + Sbjct: 151 -QADCIWEIHLLLCYSVVLEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWE----EKG 205 Query: 183 KAFGRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDFD 242 + G + +IYT+A+G + Y I L E W H I SGE+ HGP EI + Sbjct: 206 RQLGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPG 265 Query: 243 VPFIVIKGLGETRPLDDRAYDFCVKYSDKTILID-AETFDLEGISPDVRPYFTNLVAGVV 301 VPF+ + G E+R +RA +F + +D I+ID AE IS + P+ + V Sbjct: 266 VPFLFLLGNDESRHTTERAINFVKQRTDNVIVIDYAE------ISQGLHPWLAPFLMFVP 319 Query: 302 LRQYAQHLSEETGHALSVRRY 322 + +LS H RRY Sbjct: 320 MEWLCYYLSIYKDHNPDERRY 340 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,996,939 Number of extensions: 1043037 Number of successful extensions: 1634 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 1617 Number of HSP's successfully gapped: 26 Length of query: 328 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 221 Effective length of database: 26,037,693 Effective search space: 5754330153 Effective search space used: 5754330153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)