BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0037.p (410 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjiB [Q] COG2124 Cytochrome P450 322 9e-88 BS_cypA [Q] COG2124 Cytochrome P450 215 1e-55 BS_bioI [Q] COG2124 Cytochrome P450 201 1e-51 Rv0766c [Q] COG2124 Cytochrome P450 189 6e-48 MT0790 [Q] COG2124 Cytochrome P450 189 6e-48 >BS_yjiB [Q] COG2124 Cytochrome P450 Length = 396 Score = 322 bits (824), Expect = 9e-88, Method: Compositional matrix adjust. Identities = 174/364 (47%), Positives = 234/364 (64%), Gaps = 12/364 (3%) Query: 23 TKQAQWEPYAWYKDMRDRNPVYYDAEQDVWNVFSYDHVKRVLFDHELFSNKKERSLIPTP 82 KQ + P+ WY+ MR PV +D E VW+VF YD VK+V+ D ELFS S +P Sbjct: 19 NKQDAYHPFPWYESMRKDAPVSFDEENQVWSVFLYDDVKKVVGDKELFS-----SCMPQQ 73 Query: 83 IQLDNRSNVNLVDPPEHRKRRALLTQAFTPRSLKEWEPRIQEIVDELITNMEGAPVIDIV 142 S +N+ DPP+H K R+++ +AFTPR +K+WEPRIQEI DELI +G D+V Sbjct: 74 TSSIGNSIINM-DPPKHTKIRSVVNKAFTPRVMKQWEPRIQEITDELIQKFQGRSEFDLV 132 Query: 143 QKLAIPLPVTVIADLLGVPSRARGQIKAWSDILF-LPYNK--KAYTDIEQQKGRAMKEFA 199 + PLPV VI++LLGVPS Q KAWSD+L P +K +A +++ + +E A Sbjct: 133 HDFSYPLPVIVISELLGVPSAHMEQFKAWSDLLVSTPKDKSEEAEKAFLEERDKCEEELA 192 Query: 200 EFLYPIVLEKRKHPADDIISDLTQAELEGEKLTDEEVVRSAIGLLGAGNETTTTLISNIF 259 F I+ EKR P DIIS L +AE GEKL+ EE++ LL AGNETTT LISN Sbjct: 193 AFFAGIIEEKRNKPEQDIISILVEAEETGEKLSGEELIPFCTLLLVAGNETTTNLISNAM 252 Query: 260 YCMLFDQPGVYQELRADLSLVPKLIEEVLRFRFGATIDRKVAQ-DTNVFGPEMKAGQVVV 318 Y +L + PGVY+ELR+ L+P+ +EE LRFR A + R++A+ DT + G +K G +V+ Sbjct: 253 YSIL-ETPGVYEELRSHPELMPQAVEEALRFRAPAPVLRRIAKRDTEIGGHLIKEGDMVL 311 Query: 319 AWIGAANRDESHFTRADVFDIHRPGNQQHMTFGAGPHFCLGAPLARLEANMALASFVRRF 378 A++ +ANRDE+ F R +FDI R N H+ FG G HFCLGAPLARLEAN+AL S + F Sbjct: 312 AFVASANRDEAKFDRPHMFDIRRHPN-PHIAFGHGIHFCLGAPLARLEANIALTSLISAF 370 Query: 379 PDIQ 382 P ++ Sbjct: 371 PHME 374 >BS_cypA [Q] COG2124 Cytochrome P450 Length = 410 Score = 215 bits (547), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 134/388 (34%), Positives = 216/388 (55%), Gaps = 25/388 (6%) Query: 10 RQVILLNEISKLATKQAQWEPYAWYKDMRDRNPVY---YDAEQDVWNVFSYDHVKRVLFD 66 + V +L E S+L+++ + E Y +YK++R +Y A W + YD +L + Sbjct: 7 KSVTILTE-SQLSSRAFKDEAYEFYKELRKSQALYPLSLGALGKGWLISRYDDAIHLLKN 65 Query: 67 HEL-------FSNKKERSLIPTPIQLDNRSNVNLVDPPEHRKRRALLTQAFTPRSLKEWE 119 +L F+ K++R + + + +N DPP+H + R L+ +AFT R + + E Sbjct: 66 EKLKKNYENVFTAKEKRPALLKNEETLTKHMLN-SDPPDHNRLRTLVQKAFTHRMILQLE 124 Query: 120 PRIQEIVDELITNMEGAPVIDIVQKLAIPLPVTVIADLLGVPSRARGQIKAWSDILFLPY 179 +IQ I D L+ ++ +++V A PLP+ VI+++LG+P R + + WS + Sbjct: 125 DKIQHIADSLLDKVQPNKFMNLVDDYAFPLPIIVISEMLGIPLEDRQKFRVWSQAII--- 181 Query: 180 NKKAYTDIE---QQKGRAMKEFAEFLYPIVLEKRKHPADDIISDLTQAELEGEKLTDEEV 236 ++D Q+ + EF E+L +V +KR+ PA D+IS L QAE EG +L+ EE+ Sbjct: 182 ---DFSDAPERLQENDHLLGEFVEYLESLVRKKRREPAGDLISALIQAESEGTQLSTEEL 238 Query: 237 VRSAIGLLGAGNETTTTLISNIFYCMLFDQPGVYQELRADLSLVPKLIEEVLRFR--FGA 294 + L+ AG+ETT LI+N+ Y ++ + ++LR L+ IEE LRF Sbjct: 239 YSMIMLLIVAGHETTVNLITNMTYALMCHHDQL-EKLRQQPDLMNSAIEEALRFHSPVEL 297 Query: 295 TIDRKVAQDTNVFGPEMKAGQVVVAWIGAANRDESHFTRADVFDIHRPGNQQHMTFGAGP 354 T R A+ + G E+K V++ + +ANRDE F AD+FDI R N +H+ FG G Sbjct: 298 TTIRWTAEPFILHGQEIKRKDVIIISLASANRDEKIFPNADIFDIERKNN-RHIAFGHGN 356 Query: 355 HFCLGAPLARLEANMALASFVRRFPDIQ 382 HFCLGA LARLEA +A+++ +RR P+IQ Sbjct: 357 HFCLGAQLARLEAKIAISTLLRRCPNIQ 384 >BS_bioI [Q] COG2124 Cytochrome P450 Length = 395 Score = 201 bits (512), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 128/380 (33%), Positives = 205/380 (53%), Gaps = 24/380 (6%) Query: 19 SKLATKQAQWEPYAWYKDMRDRNPVYYDA--EQDVWNVFSYDHVKRVLFDHELFSNKKER 76 S A+ + PY++Y +R +P+Y + + W V Y+ +L D K R Sbjct: 5 SSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGYEETAAILKDARF----KVR 60 Query: 77 SLIP-TPIQLDNRSNVN-----LVDPPEHRKRRALLTQAFTPRSLKEWEPRIQEIVDELI 130 + +P + + + S+V + P+HR+ R L + AFTPR+ + ++P I E V L+ Sbjct: 61 TPLPESSTKYQDLSHVQNQMMLFQNQPDHRRLRTLASGAFTPRTTESYQPYIIETVHHLL 120 Query: 131 TNMEGAPVIDIVQKLAIPLPVTVIADLLGVPSRARGQIKAWSDILF----LPYNKKAYTD 186 ++G ++++ A PL VIA+++GVP R Q+K W+ L ++KA T+ Sbjct: 121 DQVQGKKKMEVISDFAFPLASFVIANIIGVPEEDREQLKEWAASLIQTIDFTRSRKALTE 180 Query: 187 IEQQKGRAMKEFAEFLYPIVLEKRKHPADDIISDLTQAELEGEKLTDEEVVRSAIGLLGA 246 +AM F E ++ ++++HP D+IS L + E +KLT+EE + I L A Sbjct: 181 GNIMAVQAMAYFKE----LIQKRKRHPQQDMISMLLKGR-EKDKLTEEEAASTCILLAIA 235 Query: 247 GNETTTTLISNIFYCMLFDQPGVYQELRADLSLVPKLIEEVLRFRFGATIDRKVA-QDTN 305 G+ETT LISN C+L P +LR + L+ +EE LR+ + +VA +D + Sbjct: 236 GHETTVNLISNSVLCLL-QHPEQLLKLRENPDLIGTAVEECLRYESPTQMTARVASEDID 294 Query: 306 VFGPEMKAGQVVVAWIGAANRDESHFTRADVFDIHRPGNQQHMTFGAGPHFCLGAPLARL 365 + G ++ G+ V +GAANRD S FT DVFDI R N H++FG G H CLG+ LARL Sbjct: 295 ICGVTIRQGEQVYLLLGAANRDPSIFTNPDVFDITRSPN-PHLSFGHGHHVCLGSSLARL 353 Query: 366 EANMALASFVRRFPDIQQTD 385 EA +A+ + ++R P + D Sbjct: 354 EAQIAINTLLQRMPSLNLAD 373 >Rv0766c [Q] COG2124 Cytochrome P450 Length = 402 Score = 189 bits (481), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 116/360 (32%), Positives = 197/360 (54%), Gaps = 6/360 (1%) Query: 29 EPYAWYKDMRDRNPVYYDAEQDVWNVFSYDHVKRVLFDHELFSNKKERSLIPTPIQLDNR 88 +PY +Y+ +RD P+Y + E++ W V + V + D SN SL P+ + Sbjct: 22 DPYPYYRRLRDEAPLYRNEERNFWAVSRHHDVLQGFRDSTALSNAYGVSLDPSSRTSEAY 81 Query: 89 SNVNLV--DPPEHRKRRALLTQAFTPRSLKEWEPRIQEIVDELITNMEGAPVIDIVQKLA 146 ++++ D P H + R L+++ FTPR ++E EP++ E+ + + D V + A Sbjct: 82 RVMSMLAMDDPAHLRMRTLVSKGFTPRRIRELEPQVLELARIHLDSALQTESFDFVAEFA 141 Query: 147 IPLPVTVIADLLGVPSRARGQIKAWSDILFLPYNKKAYTDIEQQKGRAMKEFAEFLYPIV 206 LP+ VI++L+GVP R +I+A +D + + + D+ A E + ++ Sbjct: 142 GKLPMDVISELIGVPDTDRARIRALADAVL--HREDGVADVPPPAMAASIELMRYYADLI 199 Query: 207 LEKRKHPADDIISDLTQAELEGEKLTDEEVVRSAIGLLGAGNETTTTLISNIFYCMLFDQ 266 E R+ PA+++ S L AEL+G++L+D+E++ ++ AGNETTT L++N Y Sbjct: 200 AEFRRRPANNLTSALLAAELDGDRLSDQEIMAFLFLMVIAGNETTTKLLANAVYWAAH-H 258 Query: 267 PGVYQELRADLSLVPKLIEEVLRFRFGATI-DRKVAQDTNVFGPEMKAGQVVVAWIGAAN 325 PG + AD S +P +EE LR+ + I R VA D ++ + G+V++ G+AN Sbjct: 259 PGQLARVFADHSRIPMWVEETLRYDTSSQILARTVAHDLTLYDTTIPEGEVLLLLPGSAN 318 Query: 326 RDESHFTRADVFDIHRPGNQQHMTFGAGPHFCLGAPLARLEANMALASFVRRFPDIQQTD 385 RD+ F D + I R + ++FG+G HFCLGA LAR+EA +AL + +RR + + D Sbjct: 319 RDDRVFDDPDDYRIGREIGCKLVSFGSGAHFCLGAHLARMEARVALGALLRRIRNYEVDD 378 >MT0790 [Q] COG2124 Cytochrome P450 Length = 402 Score = 189 bits (481), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 116/360 (32%), Positives = 197/360 (54%), Gaps = 6/360 (1%) Query: 29 EPYAWYKDMRDRNPVYYDAEQDVWNVFSYDHVKRVLFDHELFSNKKERSLIPTPIQLDNR 88 +PY +Y+ +RD P+Y + E++ W V + V + D SN SL P+ + Sbjct: 22 DPYPYYRRLRDEAPLYRNEERNFWAVSRHHDVLQGFRDSTALSNAYGVSLDPSSRTSEAY 81 Query: 89 SNVNLV--DPPEHRKRRALLTQAFTPRSLKEWEPRIQEIVDELITNMEGAPVIDIVQKLA 146 ++++ D P H + R L+++ FTPR ++E EP++ E+ + + D V + A Sbjct: 82 RVMSMLAMDDPAHLRMRTLVSKGFTPRRIRELEPQVLELARIHLDSALQTESFDFVAEFA 141 Query: 147 IPLPVTVIADLLGVPSRARGQIKAWSDILFLPYNKKAYTDIEQQKGRAMKEFAEFLYPIV 206 LP+ VI++L+GVP R +I+A +D + + + D+ A E + ++ Sbjct: 142 GKLPMDVISELIGVPDTDRARIRALADAVL--HREDGVADVPPPAMAASIELMRYYADLI 199 Query: 207 LEKRKHPADDIISDLTQAELEGEKLTDEEVVRSAIGLLGAGNETTTTLISNIFYCMLFDQ 266 E R+ PA+++ S L AEL+G++L+D+E++ ++ AGNETTT L++N Y Sbjct: 200 AEFRRRPANNLTSALLAAELDGDRLSDQEIMAFLFLMVIAGNETTTKLLANAVYWAAH-H 258 Query: 267 PGVYQELRADLSLVPKLIEEVLRFRFGATI-DRKVAQDTNVFGPEMKAGQVVVAWIGAAN 325 PG + AD S +P +EE LR+ + I R VA D ++ + G+V++ G+AN Sbjct: 259 PGQLARVFADHSRIPMWVEETLRYDTSSQILARTVAHDLTLYDTTIPEGEVLLLLPGSAN 318 Query: 326 RDESHFTRADVFDIHRPGNQQHMTFGAGPHFCLGAPLARLEANMALASFVRRFPDIQQTD 385 RD+ F D + I R + ++FG+G HFCLGA LAR+EA +AL + +RR + + D Sbjct: 319 RDDRVFDDPDDYRIGREIGCKLVSFGSGAHFCLGAHLARMEARVALGALLRRIRNYEVDD 378 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,411,403 Number of extensions: 1258207 Number of successful extensions: 3133 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 3025 Number of HSP's successfully gapped: 37 Length of query: 410 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 301 Effective length of database: 25,807,025 Effective search space: 7767914525 Effective search space used: 7767914525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)