BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0031.p (330 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0157 [K] COG2207 AraC-type DNA-binding domain-containing prot... 84 3e-16 lin2983 [K] COG2207 AraC-type DNA-binding domain-containing prot... 72 1e-12 CAC1451 [K] COG2207 AraC-type DNA-binding domain-containing prot... 66 8e-11 lin2267 [K] COG2207 AraC-type DNA-binding domain-containing prot... 65 2e-10 SPy1587 [T] COG4753 Response regulator containing CheY-like rece... 62 1e-09 >lin0157 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 277 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 67/271 (24%), Positives = 132/271 (48%), Gaps = 38/271 (14%) Query: 63 RFKDRHRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHSE----DLTNTDSA 118 R + H HD++E++ +C+G + +I G ++ ++ I + H + +TN Sbjct: 25 RVGENHHHDFLEISIICEGNVIYDIEGERVALEKGDMLIFNPGVNHYDITEPGMTNAQLH 84 Query: 119 VVFLCMS-ETFFDEIFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSDNNQMYNLISQ 177 + F + E + F F + L+K++ D + + ++ Q Sbjct: 85 IGFRNFALEGYSRNTF----------PFKKAFLRKKE------------DESAILSISKQ 122 Query: 178 VVEEAESKRKGYDYLLKGLMMR-IMDVLANSY-------QIQLSKLEKQRKDEILFQELE 229 ++EE ++++ GYD +LK +M+ I+ VL + ++LS E+Q+ ++L E+ Sbjct: 123 IIEEKDAEKPGYDLILKAFVMQLIIHVLREATPEQLENNGVKLSTDEQQK--QMLVNEII 180 Query: 230 KYLHQKYKE-VTIEELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKL 288 Y+ + + E V++ L + + Y +++ K+ T S +L IRL++AEE+L + Sbjct: 181 HYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDV 240 Query: 289 PVHQIIHEVGYQNKGYFYKIFVQRYGITPKE 319 V Q + VGY + YF K+F + YG P E Sbjct: 241 TVKQAANMVGYNDAYYFSKLFKKYYGFPPSE 271 >lin2983 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 326 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 75/311 (24%), Positives = 131/311 (42%), Gaps = 47/311 (15%) Query: 38 PNGRISFVIENLAQ--------RTYRVDIDPTRRFKDR--HRHDYVELAYVCKGQLPMEI 87 PNG SF+ EN + + + I RF D H H ++EL Y+ G I Sbjct: 29 PNG--SFLKENGSMELLNDYFFKNKDIYISKHNRFADYPYHTHQFLELNYMYSGSSEQVI 86 Query: 88 SGNHYIFQRDEVFIIDRNCIHSEDLTNTDSAVVFLCM----------------SETFFDE 131 GN E+ ++D C HS T ++ + + FD Sbjct: 87 EGNKETLHEGELLLLDSACHHSIKATGEKDILINILFRDQQISLEWLTSIKKKNSLLFDF 146 Query: 132 IFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSDNNQMYNLISQVVEEAESKRKGYDY 191 + S + E+ L+++I + I+QF+ NQ+ + +EE S R Y Sbjct: 147 LLHSSVSEQSLKKYIIFQSAQIPHIQQFL--------NQI--ITEYFLEEEFSSRIITLY 196 Query: 192 L--LKGLMMRIMDV-LANSYQIQLSKLEKQRKDEILFQELEKYLHQKYKEVTIEELVLKY 248 L L ++R D LA+ L+ + +L E+ K + Y+ +T+ Sbjct: 197 LPILFTELVRKCDTYLAD----DLTSAQSSTTSTML--EVIKLIESNYRTITLTSAADTL 250 Query: 249 HYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQIIHEVGYQNKGYFYKI 308 YN +Y + IKK T +F++ + ++ A ++ + P+ II +VG+ NK YFY + Sbjct: 251 GYNKNYLSNFIKKNTNFTFTELVNKQKMLAANLLIETTSTPIELIIEKVGFSNKTYFYSL 310 Query: 309 FVQRYGITPKE 319 + + + + P E Sbjct: 311 YKKTFNLLPNE 321 >CAC1451 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 295 Score = 65.9 bits (159), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 66/269 (24%), Positives = 131/269 (48%), Gaps = 16/269 (5%) Query: 54 YRVDIDPTRRFKDRHRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHSEDLT 113 ++ DI FK RH H+++++ Y G +E + N + + II+ N +H L Sbjct: 23 FKDDILNGENFK-RHWHEHLQIYYFTAGTAYLECAQNKFDVTTGSIAIINSNELHY--LE 79 Query: 114 NTDSAVVFLCMSETFFDEIFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSDNN-QMY 172 + + + F + + FL +E+ LQ L + + F + +N+ Q+ Sbjct: 80 SHSNNLKFYTIR---IEPTFLFSNKEDLLQTKYLAPLALNR-----ITFKNLIENDLQIL 131 Query: 173 NLISQVVEEAESKRKGYDYLLKGLMMRIMDVLANSYQIQ-LSKLEKQRKDEIL--FQELE 229 I +++E SK GY+ +K + +++ L Y + L++ E + + IL FQ + Sbjct: 132 ECIKTILKEYSSKNIGYELAVKANIYQLIVYLLRGYVDKILNENEFEERKRILNKFQCVF 191 Query: 230 KYLHQKYKE-VTIEELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKL 288 + + +KY E +T+ EL +++ +F R K+ T + + ++ IRL K+ L + L Sbjct: 192 RLIEEKYTEKLTLNELADCVNFSTHHFCRTFKQMTGKTTTDYINGIRLYKSTNYLKQTDL 251 Query: 289 PVHQIIHEVGYQNKGYFYKIFVQRYGITP 317 + +I + G+ N YF ++F + Y +TP Sbjct: 252 NITEIAIKCGFDNNNYFSRLFKKHYDMTP 280 >lin2267 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 292 Score = 65.1 bits (157), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 59/265 (22%), Positives = 114/265 (43%), Gaps = 28/265 (10%) Query: 68 HRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHSEDLTNTDSAVVFLCMSET 127 H H +E+ Y KG L + ++ E+ II+ +H L + +S + + + Sbjct: 30 HWHKEIEIIYALKGSLNLGVNDMPIQLNEGEIQIINGGDVHY-FLASPNSERIVIQFDLS 88 Query: 128 FFDEIFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSDNNQMYNLISQVVEEAESKRK 187 F E + Q L+E + + R P ++ +LI + E+ + Sbjct: 89 LFQEEMQMDGQNGTLREML-------TEMAHLSREWPEETTAKVQSLIRNIHLESSNDSP 141 Query: 188 GYDYLLKGLMMRIM--------------DVLANSYQIQLSKLEKQRKDEILFQELEKYLH 233 G Y+LK ++ I+ D + + + SK Q+ D+I Y+ Sbjct: 142 GKHYILKADLLAIIGLIYREIPQKKTQPDSVISEKAVLKSKETLQKLDQIF-----SYVE 196 Query: 234 QKYKE-VTIEELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQ 292 + Y+E V+++E+ ++ YF + K+ T ++F FL RL+KA+ ML+ PV + Sbjct: 197 KHYQEPVSLQEVADYTGFSTYYFTKFFKRNTGMTFVTFLNDYRLNKAKWMLLNEAFPVTE 256 Query: 293 IIHEVGYQNKGYFYKIFVQRYGITP 317 + G+ + F+ F + G+ P Sbjct: 257 VAELAGFSSVKTFHHAFKRAMGVAP 281 >SPy1587 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 494 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 27/84 (32%), Positives = 53/84 (63%) Query: 236 YKEVTIEELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQIIH 295 ++E+T++ + + H N Y + K T+ SF+Q+L +R+ KA+++L+ + +++I + Sbjct: 395 HQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSINEIAY 454 Query: 296 EVGYQNKGYFYKIFVQRYGITPKE 319 E GY YF K+F + G++PKE Sbjct: 455 ETGYNTNHYFIKMFKKLNGLSPKE 478 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,392,146 Number of extensions: 887099 Number of successful extensions: 4497 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 4495 Number of HSP's successfully gapped: 32 Length of query: 330 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 223 Effective length of database: 26,037,693 Effective search space: 5806405539 Effective search space used: 5806405539 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)