BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0030.p (496 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase 124 5e-28 STM1062 [R] COG0488 ATPase components of ABC transporters with d... 124 5e-28 SMb21655 [G] COG3250 Beta-galactosidase/beta-glucuronidase 115 1e-25 ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 104 4e-22 ECs3958 [G] COG3250 Beta-galactosidase/beta-glucuronidase 104 4e-22 >TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 563 Score = 124 bits (310), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 114/426 (26%), Positives = 185/426 (43%), Gaps = 61/426 (14%) Query: 28 IAKKRSAQAVSGSKKGYFSD-----GAYDYVKKFYVPEEYKDKRVTFEFEGVYMHAMVYI 82 + K AV GS + D G + Y FYVP+E K + F V V++ Sbjct: 23 VTSKDRPIAVPGSWNEQYQDLCYEEGPFTYKTTFYVPKELSQKHIRLYFAAVNTDCEVFL 82 Query: 83 NGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSH----------DDS--------- 123 NG+ G++ Y F + +K G ENE++VV ++ DS Sbjct: 83 NGEKVGENHIEYLPFEVDVTGKVKSG-ENELRVVVENRLKVGGFPSKVPDSGTHTVGFFG 141 Query: 124 -------RWYTGTGIYRNTKIMVADLVHIAVDGVKINATDIESKHAVVVVATDVENEGIN 176 ++ GI R I D I V + ++ E K V V +V E + Sbjct: 142 SFPPANFDFFPYGGIIRPVLIEFTDHARILDIWVDTSESEPEKKLGKVKVKIEVSEEAVG 201 Query: 177 PRTVRIVTEIVDADGNSVASDTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISK 236 + ++ + + S+ F E A + W++E PYLY + Sbjct: 202 QE---MTIKLGEEEKKIRTSNRFVEGEFILENA-----------RFWSLEDPYLYPLKVE 247 Query: 237 VMDGEHVLDEETHTFGIRTLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADER 296 + E+ LD GIRT+S D E L +NG+ V L+G H + V+G T + Sbjct: 248 LEKDEYTLD-----IGIRTISWD-EKRLYLNGKPVFLKGFGKHEEFPVLGQGTFYPLMIK 301 Query: 297 RIEILKQAGFNAIRSAHHPASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWW 356 +LK N+ R++H+P S+ L DR+G+LV+DE+ + + Y Y Sbjct: 302 DFNLLKWINANSFRTSHYPYSEEWLDLADRLGILVIDEAPHVGI---TRYHYNPETQKIA 358 Query: 357 EQDIQAMVDKDYNHPSVILYSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSING 416 E +I+ M+D+ NHPSVI++S+ NE PE+ + + + + E +D TR V+ Sbjct: 359 EDNIRRMIDRHKNHPSVIMWSVANE-PESNHPDAEGFFKALYETANEMDRTRPVV----- 412 Query: 417 MVAVMN 422 MV++M+ Sbjct: 413 MVSMMD 418 >STM1062 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 635 Score = 124 bits (310), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 22/426 (5%), Positives = 67/426 (15%), Gaps = 61/426 (14%) Query: 28 IAKKRSAQAVSGSKKGYFSD-----GAYDYVKKFYVPEEYKDKRVTFEFEGVYMHAMVYI 82 + V + Y+ + + + Sbjct: 23 ELHIEDNERVCLVGRNGAGKSTLMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNIAGS 82 Query: 83 NGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSH----------DDS--------- 123 DF + + + + + + + Sbjct: 83 VYDFVAEGIEEQAEYLKRYHEISRLV-MTDPSEKNLNEMARVQEQLDHHNLWQLENRINE 141 Query: 124 -------RWYTGTGIYRNTKIMVADLVHIAVDGVKINATDIESKHAVVVVATDVENEGIN 176 + A L V ++ D + H + +E Sbjct: 142 VLAQLGLDPNAALSSLSGGWLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKT 201 Query: 177 PRTVRIVTEIVDADGNSVASDTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISK 236 + D + + + K + + Sbjct: 202 FN---GTIIFISHDRSFIRNMATRIVDLDRGKL-----------VTYPGNYDQYLLEKEE 247 Query: 237 VMDGEHVLDEETHTFGIRTLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADER 296 + E + + E+ G + E Sbjct: 248 ALRVEELQN-----AEFDRKLAQ-EEVWIRQGIKARRTRNEGRVRALKAMRRERSERREV 301 Query: 297 RIEILKQAGFNAIRSAHHPASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWW 356 Q + + + ++ + Sbjct: 302 MGTAKMQVEEATRSGKIVFEMENVDYQVEGKQLVKDFSAQVQRG---DKIALIGPNGCGK 358 Query: 357 EQDIQAMVDKDYNHPSVILYSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSING 416 ++ M+ + I E + + + + VM Sbjct: 359 TTLLKLMLGQLQADSGRIHVGTKLE-VAYFDQHRAELDPEKTVMDNLAEGKQEVM----- 412 Query: 417 MVAVMN 422 + Sbjct: 413 VNGKPR 418 >SMb21655 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 755 Score = 115 bits (288), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 101/351 (28%), Positives = 162/351 (46%), Gaps = 47/351 (13%) Query: 49 AYDYVKKFYVPEEYKDKRVTFEFEGVYMHAMVYINGDFAGQHPFGYTNFYIK-ADRFLKY 107 A+ Y + ++ + V+ F+ A+VY+NG+ H GYT F + DR L+ Sbjct: 54 AFTYQRVLAWRPDFSGREVSLVFDAAMADAVVYLNGEEIVAHRDGYTPFEARLTDRLLEG 113 Query: 108 ---------GAEN-EIKVVAKSHDDSRWYTGTGIYRNTKIMVADLVHIAVDGVKINATDI 157 G+EN EI D + T GIYR+ + V D V IA +KI D+ Sbjct: 114 DNLITVKIDGSENPEIPPFGGRID---YLTYAGIYRDVWLKVTDPVSIA--NIKIETRDV 168 Query: 158 ESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVASDTAPFTAFAGEKATSRQRLY- 216 S V + D+ N + I + +A G +A AGE T+ Q L Sbjct: 169 LSDTKAVSLRCDLSNPQGLSFSGTISALLKNAAGEVLAE-------VAGE--TTGQSLAF 219 Query: 217 ----IREPKLWNVETPYLYTCISKVMDGEHVLDEETHTFGIRTLSLDAEDGLRINGEVVK 272 ++ LW+++ P LY ++ G+ H FG RT E G R+NG +K Sbjct: 220 EMDGLKGLSLWDIDDPVLYVIEVELRTGQGSDCFAAH-FGFRTAEFTTE-GFRLNGRPLK 277 Query: 273 LRGACIHHDNGVIGASTIERADERRIEILK-QAGFNAIRSAHHPASKALLKACDRIGMLV 331 +RG H +G + A ER +I+K + N +R++H+P SK L CDRIG+LV Sbjct: 278 IRGLNRHQSFPYVGYAMGRTAQERDADIMKHRLHCNLVRTSHYPQSKWFLDHCDRIGLLV 337 Query: 332 MDESFDIWTHHKSDYDYALHFPTWWEQD----IQAMVDKDYNHPSVILYSI 378 +E W H + W+Q+ ++ M+++D+NHPS++++ + Sbjct: 338 FEE-IPGWQHIGGEE---------WKQEAIRNVRRMIERDWNHPSIVIWGV 378 >ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 104 bits (259), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 99/385 (25%), Positives = 168/385 (43%), Gaps = 51/385 (13%) Query: 52 YVKKFYVPEEYKDKRVTFEFEGVYMHAMVYINGDFAGQHPFGYTNFYIKADRFLKYGAEN 111 Y + F + + ++ K+ +F+GV + VY+NG + G + K R AE Sbjct: 125 YQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVG---------FSKGSRLT---AEF 172 Query: 112 EIKVVAKSHD-----------------DSRWYTGTGIYRNTKIMVADLVHIAVDGVKINA 154 +I + K+ D D + GI+R+ ++ L HI V+ Sbjct: 173 DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVR--- 229 Query: 155 TDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVASDTAPFTAFAGEKATSRQR 214 TD + + ++ +V E + V E DG V +A A EK TS Sbjct: 230 TDFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSA-IDHLAIEKLTSASF 288 Query: 215 LY-IREPKLWNVETPYLYTCISKVMDGE-HVLDEETHTFGIRTLSLDAEDGL-RINGEVV 271 + + +P+ W+ E+PYLY + + D +VL+ G R + + DGL IN V Sbjct: 289 AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKV--RDGLFWINNRYV 346 Query: 272 KLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHHPASKALLKACDRIGMLV 331 L G H ++ G + E+ ++++KQ N++R+AH+P + CD G+ V Sbjct: 347 MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFV 406 Query: 332 MDESFDIWTH---HKSDYDYALHFPTW---WEQDIQAMVDKDYNHPSVILYSIGNEIPET 385 M E+ D+ +H + D P W + + I + NHPS+I++S+GN E+ Sbjct: 407 MAET-DVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGN---ES 462 Query: 386 GSANGTAWGRKIAEKIRSLDSTRYV 410 G R + ++LD TR V Sbjct: 463 GYGCNI---RAMYHAAKALDDTRLV 484 >ECs3958 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 104 bits (259), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 99/385 (25%), Positives = 168/385 (43%), Gaps = 51/385 (13%) Query: 52 YVKKFYVPEEYKDKRVTFEFEGVYMHAMVYINGDFAGQHPFGYTNFYIKADRFLKYGAEN 111 Y + F + + ++ K+ +F+GV + VY+NG + G + K R AE Sbjct: 125 YQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVG---------FSKGSRLT---AEF 172 Query: 112 EIKVVAKSHD-----------------DSRWYTGTGIYRNTKIMVADLVHIAVDGVKINA 154 +I + K+ D D + GI+R+ ++ L HI V+ Sbjct: 173 DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVR--- 229 Query: 155 TDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVASDTAPFTAFAGEKATSRQR 214 TD + + ++ +V E + V E DG V +A A EK TS Sbjct: 230 TDFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSA-IDHLAIEKLTSASF 288 Query: 215 LY-IREPKLWNVETPYLYTCISKVMDGE-HVLDEETHTFGIRTLSLDAEDGL-RINGEVV 271 + + +P+ W+ E+PYLY + + D +VL+ G R + + DGL IN V Sbjct: 289 AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKV--RDGLFWINNRYV 346 Query: 272 KLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHHPASKALLKACDRIGMLV 331 L G H ++ G + E+ ++++KQ N++R+AH+P + CD G+ V Sbjct: 347 MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFV 406 Query: 332 MDESFDIWTH---HKSDYDYALHFPTW---WEQDIQAMVDKDYNHPSVILYSIGNEIPET 385 M E+ D+ +H + D P W + + I + NHPS+I++S+GN E+ Sbjct: 407 MAET-DVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGN---ES 462 Query: 386 GSANGTAWGRKIAEKIRSLDSTRYV 410 G R + ++LD TR V Sbjct: 463 GYGCNI---RAMYHAAKALDDTRLV 484 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,262,742 Number of extensions: 1652409 Number of successful extensions: 3935 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 3906 Number of HSP's successfully gapped: 16 Length of query: 496 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 385 Effective length of database: 25,576,357 Effective search space: 9846897445 Effective search space used: 9846897445 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)