BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0027.p (529 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3683 [G] COG3507 Beta-xylosidase 654 0.0 BS_xynB [G] COG3507 Beta-xylosidase 491 e-139 CC0989 [G] COG3507 Beta-xylosidase 479 e-135 CAC3452 [G] COG3507 Beta-xylosidase 461 e-129 CC2802 [G] COG3507 Beta-xylosidase 226 6e-59 >BH3683 [G] COG3507 Beta-xylosidase Length = 528 Score = 654 bits (1686), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/526 (58%), Positives = 387/526 (73%), Gaps = 1/526 (0%) Query: 1 MNTIVNPVLRGFNPDPSIVRAGDDYYIATSTFEWFPGVQIHHSRDLVHWRLLKRPLDRIS 60 +N I NP+L GF+PDPSIVR GDDYYIATSTFEWFPGV+IHHSRDL HWR + PL R S Sbjct: 2 VNRIQNPILPGFHPDPSIVRVGDDYYIATSTFEWFPGVRIHHSRDLKHWRFVSSPLTRTS 61 Query: 61 QLNMQGNGDSDGVWAPCLTYDDGLFYLIYTDVKSHKGAFKDTHNYLVTAADIEGPWSEPV 120 QL+M+GN +S G+WAPCL+Y DG FYLIYTDVK GAFKD HNYLVTA +IEGPWS+P+ Sbjct: 62 QLDMKGNMNSGGIWAPCLSYHDGTFYLIYTDVKQWHGAFKDAHNYLVTAQNIEGPWSDPI 121 Query: 121 YLNSSGFDPSLFHDDDGRKWLLNMRWDFRKEKNKFGGIVIQEYSQTEGRLIGPVXXXXXX 180 YLNSSGFDPSLFHDDDGRKWL+NM WD+RK + F GI++QEYS+ E +L+GPV Sbjct: 122 YLNSSGFDPSLFHDDDGRKWLVNMIWDYRKGNHPFAGIILQEYSEAEQKLVGPVKNIYKG 181 Query: 181 XXXXXXXXPHLYKQNGYYYLITAEGGTRYKHAVTVARAALLMGPYEIDPLNPMLTSSGNP 240 PHLYK++GYYYL+ AEGGT Y+HA T+AR+ + GPYE DP P++TS+G P Sbjct: 182 TDIQLTEGPHLYKKDGYYYLLVAEGGTEYEHAATLARSQSIDGPYETDPSYPLVTSTGQP 241 Query: 241 ALLLQKAGHASLVETRTGEWYMAHLCARPVDGFHCTLGRETALQKCYWDAEGWLRLEGGG 300 L LQKAGH SLVET+ GEWY+AHLC RP+ G +CTLGRETA+QK W +GWLR+E GG Sbjct: 242 ELALQKAGHGSLVETQNGEWYLAHLCGRPLKGKYCTLGRETAIQKVNWTEDGWLRIEDGG 301 Query: 301 NAPSAAVQAXXXXXXXXXXXXXXXXXXXXLLGNQWNTLRIPPDPDWLSLSERQGFLRLKG 360 N P V A L +QWNTLRIP DP W SL ER G LRL+G Sbjct: 302 NHPLREVTAPDLPEHPFEKEPELDDFDAPQLHHQWNTLRIPADPSWCSLEERPGHLRLRG 361 Query: 361 RESLSSCHRQSLVARRQQVFNIEAETVIEFEPENFQQLAGLILYYNTEDYVYLRITHLEQ 420 ESL+S H QSLVARRQQ F+ E ET +E++PE+FQ +AGL++YY+TED+VYL +T E+ Sbjct: 362 MESLTSVHSQSLVARRQQSFHCEVETKLEYQPESFQHMAGLVIYYDTEDHVYLHVTWHEE 421 Query: 421 QGRVIGIIQSVHGMYDELLAADLQIPDSGPVRLKAVVNRDRLQFYYAIE-DSSWCPVGEE 479 +G+ + IIQ+ G YDELLA+ + + + V LK ++R+ + Y+ E ++ W PVG Sbjct: 422 KGKCLQIIQTKGGNYDELLASPIPLAEEKAVYLKGRIHRETMHLYFKQEGEAEWQPVGPT 481 Query: 480 SHILHLSDEAKEPLRFTGTFVGICAQDLSGTRKHADFDYFIYREME 525 + H+SD++ + +RFTGTFVG+ QDLSGT+K ADFDYF Y+E++ Sbjct: 482 IDVTHMSDDSAKQVRFTGTFVGMATQDLSGTKKPADFDYFRYKELD 527 >BS_xynB [G] COG3507 Beta-xylosidase Length = 533 Score = 491 bits (1265), Expect = e-139, Method: Compositional matrix adjust. Identities = 262/531 (49%), Positives = 330/531 (62%), Gaps = 12/531 (2%) Query: 4 IVNPVLRGFNPDPSIVRAGDDYYIATSTFEWFPGVQIHHSRDLVHWRLLKRPLDRISQLN 63 I NPVL+GFNPDPSI RAG+DYYIA STFEWFPGVQIHHS+DLV+W L+ PL R+SQL+ Sbjct: 3 ITNPVLKGFNPDPSICRAGEDYYIAVSTFEWFPGVQIHHSKDLVNWHLVAHPLQRVSQLD 62 Query: 64 MQGNGDSDGVWAPCLTYDDGLFYLIYTDVKSHKGAFKDTHNYLVTAADIEGPWSEPVYLN 123 M+GN +S GVWAPCL+Y DG F+LIYTDVK GA+KD HNYLVT I G WSEP+ LN Sbjct: 63 MKGNPNSGGVWAPCLSYSDGKFWLIYTDVKVVDGAWKDCHNYLVTCETINGDWSEPIKLN 122 Query: 124 SSGFDPSLFHDDDGRKWLLNMRWDFRKEKNKFGGIVIQEYSQTEGRLIGPVXXXXXXXXX 183 SSGFD SLFHD DG+K+LLNM WD R +++ FGGIVIQEYS E +LIG Sbjct: 123 SSGFDASLFHDTDGKKYLLNMLWDHRIDRHSFGGIVIQEYSDKEQKLIGKPKVIFEGTDR 182 Query: 184 XXXXXPHLYKQNGYYYLITAEGGTRYKHAVTVARAALLMGPYEIDPLNPMLTSSGNPALL 243 PHLY YYYL+TAEGGTRY+HA T+AR+A + GPYE+ P NP+LTS +P Sbjct: 183 KLTEAPHLYHIGNYYYLLTAEGGTRYEHAATIARSANIEGPYEVHPDNPILTSWHDPGNP 242 Query: 244 LQKAGHASLVETRTGEWYMAHLCARPV----DGF-----HCTLGRETALQKCYWDAEGWL 294 LQK GHAS+V+T T EWY+AHL RP+ D +C LGRETA+QK YW E W Sbjct: 243 LQKCGHASIVQTHTDEWYLAHLTGRPIHPDDDSIFQQRGYCPLGRETAIQKLYWKDE-WP 301 Query: 295 RLEGGGNAPSAAVQAXXXXXXXXXXXXXXXXXXXXLLGNQWNTLRIPPDPDWLSLSERQG 354 + GG L + TLRIP + SL++ Sbjct: 302 YVVGGKEGSLEVDAPSIPETIFEATYPEVDEFEDSTLNINFQTLRIPFTNELGSLTQAPN 361 Query: 355 FLRLKGRESLSSCHRQSLVARRQQVFNIEAETVIEFEPENFQQLAGLILYYNTEDYVYLR 414 LRL G ESL+S Q+ VARR Q + EAET +EF PENFQQ AGL+ YYNTE++ L+ Sbjct: 362 HLRLFGHESLTSTFTQAFVARRWQSLHFEAETAVEFYPENFQQAAGLVNYYNTENWTALQ 421 Query: 415 ITHLEQQGRVIGIIQSVHGMYDELLAADLQIP-DSGPVRLKAVVNRDRLQFYYAIEDSSW 473 +TH E+ GR++ + + + + L + IP + V L+ + +D+ ++Y+ W Sbjct: 422 VTHDEELGRILELTICDNFSFSQPLNNKIVIPREVKYVYLRVNIEKDKYYYFYSFNKEDW 481 Query: 474 CPVGEESHILHLSDE-AKEPLRFTGTFVGICAQDLSGTRKHADFDYFIYRE 523 + LSD+ + FTG FVG+ QD G ADF YF Y+E Sbjct: 482 HKIDIALESKKLSDDYIRGGGFFTGAFVGMQCQDTGGNHIPADFRYFRYKE 532 >CC0989 [G] COG3507 Beta-xylosidase Length = 546 Score = 479 bits (1232), Expect = e-135, Method: Compositional matrix adjust. Identities = 263/543 (48%), Positives = 331/543 (60%), Gaps = 22/543 (4%) Query: 2 NTIVNPVLRGFNPDPSIVRAGDDYYIATSTFEWFPGVQIHHSRDLVHWRLLKRPLDRISQ 61 + I NPVLRGFNPDPSIVR G+DYYIATSTFEWFPGVQIHHSRDL +WRL+ RPL R SQ Sbjct: 4 DLIRNPVLRGFNPDPSIVRVGEDYYIATSTFEWFPGVQIHHSRDLRNWRLIGRPLRRPSQ 63 Query: 62 LNMQGNGDSDGVWAPCLTYDDGLFYLIYTDVKSH---------KGAFKDTHNYLVTAADI 112 LNM G+ D GVWAPCLT+ DG F+LI+TDVK + + +D HNYLVT I Sbjct: 64 LNMMGDPDGCGVWAPCLTHADGRFWLIFTDVKRYGRTTVGGASGASLRDFHNYLVTCETI 123 Query: 113 EGPWSEPVYLNSSGFDPSLFHDDDGRKWLLNMRWDFRKEKNKFGGIVIQEYSQTEGRLIG 172 +G WS+P++LNSSGFDPSLFHDDDGRKWL+N WD R +N+F GIV+Q +S E RL+G Sbjct: 124 DGDWSDPIHLNSSGFDPSLFHDDDGRKWLVNQLWDHRPGRNRFAGIVLQAFSVAEQRLVG 183 Query: 173 PVXXXXXXXXXXXXXXPHLYKQNGYYYLITAEGGTRYKHAVTVARAALLMGPYEIDPLNP 232 PHLYK++G+YYL+TAEGGT + HA T+AR+ + GPYE+ P Sbjct: 184 ERKVIFEGTAIGLTEAPHLYKRDGWYYLLTAEGGTGWGHAATLARSRAIDGPYELHPDTY 243 Query: 233 MLTSSGNPALLLQKAGHASLVETRTGEWYMAHLCARPVDGF-HCTLGRETALQKCYWDAE 291 +LT+ P LQ+AGH L ET GE Y+A+LC RP+ G C LGRETA+QK W A+ Sbjct: 244 LLTARDRPHAPLQRAGHGDLFETADGETYIAYLCGRPLPGRGRCVLGRETAIQKLTWGAD 303 Query: 292 GWLRLEGGGNAPSAAVQAXXXXXXXXXXXXXXXXXXXXLLGNQWNTLRIPPDPDWLSLSE 351 GW R G P+ L + LR P SL+ Sbjct: 304 GWPRTLDGSGDPTLETPGAGLVDQPWPTTPVREDFDGTTLPIDFQWLRTPYPETIFSLTA 363 Query: 352 RQGFLRLKGRESLSSCHRQSLVARRQQVFNIEAETVIEFEPENFQQLAGLILYYNTEDYV 411 R GFLRL GRESL S Q+LVARRQQ A T ++FEP +FQQ AGL+ YYN Sbjct: 364 RPGFLRLYGRESLGSVFTQALVARRQQAHCYSAATRLDFEPAHFQQAAGLVCYYNGSKLH 423 Query: 412 YLRITHLEQQGRVIGIIQSVHGMYDELLAADLQIP---DSGPVRLKAVVNRDRLQFYY-- 466 YL +TH ++ G+ + ++ D A P +GPV L+ V+ +RL+F + Sbjct: 424 YLHVTHDDEAGKHLRVMTCTP---DSPQADSFTAPIPLGAGPVDLRVEVDFERLRFGFRQ 480 Query: 467 AIEDSSWCPVGEESHILHLSDEAKEP--LRFTGTFVGICAQDLSGTRKHADFDYFIYREM 524 A D W P ++ I LSDEA P FTG FVG+ QD+SG ADFD+F Y+E Sbjct: 481 AGGDWRWLPEVFDASI--LSDEATAPGAPNFTGAFVGMACQDMSGMATAADFDWFDYQER 538 Query: 525 EWI 527 +++ Sbjct: 539 DYV 541 >CAC3452 [G] COG3507 Beta-xylosidase Length = 533 Score = 461 bits (1185), Expect = e-129, Method: Compositional matrix adjust. Identities = 248/533 (46%), Positives = 322/533 (60%), Gaps = 13/533 (2%) Query: 4 IVNPVLRGFNPDPSIVRAGDDYYIATSTFEWFPGVQIHHSRDLVHWRLLKRPLDRISQLN 63 I NP+LRGFNPDPSI RA DYYIATSTFEWFPGVQIHHS+DLV+W L+ PL+R S L+ Sbjct: 2 IKNPILRGFNPDPSICRADTDYYIATSTFEWFPGVQIHHSKDLVNWHLVAHPLNRTSLLD 61 Query: 64 MQGNGDSDGVWAPCLTYDDGLFYLIYTDVKSHKGAFKDTHNYLVTAADIEGPWSEPVYLN 123 M+GN +S G+WAP L+Y DG F+LIYTDVK G +KD HNYL T ++G WS+P+ LN Sbjct: 62 MKGNPNSGGIWAPDLSYHDGKFWLIYTDVKVTDGMWKDCHNYLTTCESVDGVWSDPITLN 121 Query: 124 SSGFDPSLFHDDDGRKWLLNMRWDFRKEKNKFGGIVIQEYSQTEGRLIGPVXXXXXXXXX 183 SGFD SLFHD+DG+K+L+NM WD R + F GIV+QEYS E +LIG Sbjct: 122 GSGFDASLFHDNDGKKYLVNMYWDQRTYNHNFYGIVLQEYSDKEKKLIGKAKIIYKGTDI 181 Query: 184 XXXXXPHLYKQNGYYYLITAEGGTRYKHAVTVARAALLMGPYEIDPLNPMLTSSGNPALL 243 PH+Y YYYL TAEGGT Y+H+ TVAR+ + GPYEIDP P+LTS +P Sbjct: 182 KYTEGPHIYHIGDYYYLFTAEGGTTYEHSETVARSKNIDGPYEIDPEYPLLTSWHDPRNS 241 Query: 244 LQKAGHASLVETRTGEWYMAHLCARPV---------DGFHCTLGRETALQKCYWDAEGWL 294 LQK GHASLV T T EWY+AHL RP+ +C LGRET++Q+ W + W Sbjct: 242 LQKCGHASLVHTHTDEWYLAHLVGRPLPVGNQPVLEQRGYCPLGRETSIQRIEW-VDNWP 300 Query: 295 RLEGGGNAPSAAVQAXXXXXXXXXXXXXXXXXXXXLLGNQWNTLRIPPDPDWLSLSERQG 354 R+ GG L + +LRIP + SL ++G Sbjct: 301 RVVGGKQGSVNVEAPKIPEVKWEKTYDEKDNFDSDKLNINFQSLRIPLTENIASLKAKKG 360 Query: 355 FLRLKGRESLSSCHRQSLVARRQQVFNIEAETVIEFEPENFQQLAGLILYYNTEDYVYLR 414 LRL G+ESL+S Q+ +ARR Q F +A T + F P+ FQQ AGL YYNTE++ ++ Sbjct: 361 NLRLYGKESLTSTFTQAFIARRWQSFKFDASTSVSFSPDTFQQAAGLTCYYNTENWSTIQ 420 Query: 415 ITHLEQQGRVIGIIQSVHGMYDELLAAD-LQIP-DSGPVRLKAVVNRDRLQFYYAIEDSS 472 +T E +GRVI I+ + +D L ++ + IP D + LK V + Q+ Y+ + + Sbjct: 421 VTWNEDKGRVIDIVCCDNFHFDMPLKSNVIPIPKDVEYIHLKVEVRVETYQYSYSFDGIN 480 Query: 473 WCPVGEESHILHLSDE-AKEPLRFTGTFVGICAQDLSGTRKHADFDYFIYREM 524 W V LSD+ + FTG FVGI D++G K ADFDYF Y+E+ Sbjct: 481 WSKVPAIFESRKLSDDYVQGGGFFTGAFVGINCIDITGNNKPADFDYFCYKEL 533 >CC2802 [G] COG3507 Beta-xylosidase Length = 548 Score = 226 bits (577), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 171/529 (32%), Positives = 241/529 (45%), Gaps = 46/529 (8%) Query: 6 NPVLRGFNPDPSIVRAGDDYYIATSTFEWFPGVQIHHSRDLVHWRLLKRPLDRISQLNMQ 65 NPV++GF PDPS+ RAG D+Y+ STF WFPG+ + HS DLVHW + +DR L+ + Sbjct: 37 NPVVQGFYPDPSVTRAGKDFYLVNSTFAWFPGLPVFHSTDLVHWEQIGNAIDRPGMLDFK 96 Query: 66 GNGDSDGVWAPCLTYDDGLFYLIYTDVKSHKGAFKDTHNYLVTAADIEGPWSEPVYL-NS 124 G S V+AP + G+FY++ T V NYL+TA D +GPWS+PV+L Sbjct: 97 RLGLSRAVFAPAIQAHQGMFYILNTCVDCGG-------NYLITAKDPKGPWSDPVWLPKV 149 Query: 125 SGFDPSLFHDDD---GRKWLLNM-RWDFRKEKNKFGGIVIQEYSQTEGRLIGPVXXXXXX 180 G DPS+F DD+ GR W++N E + I EY +GP Sbjct: 150 GGIDPSIFFDDEDGGGRVWIVNNDGPPGPPEYPGHRALWIHEYDPKTQTTVGPATLLLDK 209 Query: 181 XXXXXXXX-----PHLYKQNGYYYLITAEGGTRYKHAVTVARAALLMGPYEIDPLNPMLT 235 PH+ K++G+YYL AEGGT H+ V R+ + GPY NP+LT Sbjct: 210 GVDPATKPIWPEGPHILKKDGWYYLTAAEGGTAEGHSQVVLRSKSVTGPYVPYTNNPILT 269 Query: 236 SSGNP---ALLLQKAGHASLVETRTGEWYMAHLCARPVDGFHCTLGRETALQKCYWDAEG 292 P L + AGHA +V+T G+W+ L RP GRET L W +G Sbjct: 270 QRDLPRDRPLPITSAGHADIVDTPDGKWWATFLAVRPYGDDLYNTGRETFLLPVEWK-DG 328 Query: 293 W-LRLEGGGNAPSAAVQAXXXXXXXXXXXXXXXXXXXXLLGNQ----WNTLRIPPDPDWL 347 W + LE G P A A G + W T+RIP W Sbjct: 329 WPVILENGKTIPYVA-DAPKLPALKTAPTSGPFKAAETFEGPELPMSWMTMRIPSKRWW- 386 Query: 348 SLSERQGFLRLKGRESLSSCHRQ-SLVARRQQVFNIEAETVIEFEPENFQQLAGLILYYN 406 S + G L L+ R+ H Q S+ ARRQQ N T + F+P+ AGL+ N Sbjct: 387 --SVKDGTLSLEARKDRVGGHEQPSIWARRQQHMNASVSTTVRFDPKADGDKAGLVAVQN 444 Query: 407 TEDYVYLRITHLEQQGRVIGIIQSVHGMYDELLAADLQIPDSGPVRLKAVVNRD------ 460 + Y ++ + ++ G+ + ++ D L D + PV LK+ + D Sbjct: 445 DDFYWFVGL--VQAGGKTLVRVEKRTSSKDPL---DGAVVAEKPVSLKSGQSLDLKIAAR 499 Query: 461 --RLQFYYAIEDSSWCPVGEESHILHLSDEAKEPLRFTGTFVGICAQDL 507 L F YA W + ++ LS K F GT +G+ A + Sbjct: 500 GGALDFAYATAPGQWQTLKADADGTTLS--TKVAGGFVGTMLGVYAHGV 546 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,561,178 Number of extensions: 1614397 Number of successful extensions: 2006 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1980 Number of HSP's successfully gapped: 8 Length of query: 529 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 418 Effective length of database: 25,576,357 Effective search space: 10690917226 Effective search space used: 10690917226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)