BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0025.p (526 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 113 8e-25 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 107 5e-23 BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 99 2e-20 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 75 2e-13 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 62 2e-09 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 113 bits (282), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 88/269 (32%), Positives = 137/269 (50%), Gaps = 35/269 (13%) Query: 84 YVEW-NGGTNYLNQLNLKIAANEMPDLFVPQQGLED----SLAKNGAIADLTELIPEYAP 138 +++W N +++ + NL I++ ++PD + G D + AK G I + +LI +Y P Sbjct: 79 HIDWTNYQSDFAEKRNLDISSGDLPDA-IHNDGASDVDLMNWAKKGVIIPVEDLIDKYMP 137 Query: 139 NVWAAIPEDIWKVVKANDPTGQGKIYYIPNVVEYG---------RYAGLIRQDWLDKLGL 189 N+ + E KA G IY P + E G I +DWL KLGL Sbjct: 138 NLKKILDEK--PEYKALMTAPDGHIYSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGL 195 Query: 190 SMPKTQDEYVKVLEAFRDQDPNGNGQKDEIPTG-----GREQARWMDHLFAMYGVAMFEG 244 MPKT D+ +KVLEAF++ DPNGNG+ DEIP G E ++ LFA +G+ + Sbjct: 196 EMPKTTDDLIKVLEAFKNGDPNGNGEADEIPFSFISGNGNEDFKF---LFAAFGIGDNDD 252 Query: 245 YPEWDIYDGELTYSAVTPNMKAALEFISKLYKEGLIDKESLLNSKDKWDGKIQSDKVGNY 304 + DG++ ++A N K ++FI +L ++GLIDKE+ + + + K K G Y Sbjct: 253 HLVVG-NDGKVDFTADNDNYKEGVKFIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVY 311 Query: 305 FHWVEQGNLIFENLYKNTGVKAEFSVLPI 333 F W ++ N+ TG + VLP+ Sbjct: 312 FTW-DKNNV--------TGSNESYDVLPV 331 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 107 bits (267), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 126/463 (27%), Positives = 203/463 (43%), Gaps = 60/463 (12%) Query: 85 VEWNGGTNYLNQLNLKIAANEMPDLFV---PQQGLEDSLAKNGAIADLTELIPEYAPNVW 141 V W ++Y ++ N+ +A+ EMP V G S+ K GA +L+ + +Y N+ Sbjct: 70 VTWVPDSSYNDKFNIVMASGEMPHAIVIKDKSAGFIKSV-KAGAFWELSPYLKDYK-NLS 127 Query: 142 AAIPEDIWKVVKANDPTGQGKIYYIPNVVEYGRYAGLIRQDWLDKLGLSMPKTQDEYVKV 201 A E I K N G++Y I + R +IR DWL +GL MP+T D++ +V Sbjct: 128 QA-DEKILKNSSVN-----GEVYGIYRTRDLIRACMIIRTDWLKNVGLDMPETLDDFYEV 181 Query: 202 LEAFRDQDPNGNGQKDEIPTGGREQARWM--------DHLFAMYGVAMFEGYPEWDIYDG 253 L+AF+++DP+GNG+ D T G +WM D L +G G + +G Sbjct: 182 LKAFKEKDPDGNGKDD---TYGMVVPKWMGLGNGSPWDVLQIWFGAPNRYG-----VENG 233 Query: 254 ELTYSAVTPNMKAALEFISKLYKEGLIDKESLLNSKDKWDGKIQSDKVGNYFHWVEQGNL 313 +L T AL F KLY EGLI+K+ + KW+ + K G + + Sbjct: 234 KLIPDFTTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVVKGKAGVIVDTGSRASQ 293 Query: 314 IFENLYK----NTGVKAEFSVLPIPE------VEGYKGFYTMKKIASPLWVVKNNKDEEK 363 I + + N + L P GY G T+ K + K E++ Sbjct: 294 IQSAMEEADESNKDIIDIVGSLEGPNGKRTFPTSGYSGMITIPKSSV--------KTEKE 345 Query: 364 LMATLKLLNNMYDKSNWKDLFLGVEGMHYTMKDGKAVKLPDDKST---QENQILNPATSV 420 L L L+ M DK GV+G +Y +KDG L + + + + + S+ Sbjct: 346 LKEVLSFLDKMNDKEAQILTNNGVKGRNYELKDGVFTSLEKNNKSLLYEHEGLAQFSMSI 405 Query: 421 STLDFTIDLFNSTVSEDSKWAIDQVIRN-MQEAQQYAKVIAGDGMPASVYDGY-PDINNR 478 ++ I ED K + Q ++ + E ++ A + + + VY ++N Sbjct: 406 PKSEYYI--------EDQKTKLFQHRKDIITEGEKIAVFNPAESLVSDVYTQKGAQLDNI 457 Query: 479 TLYAEYASKIIIGQYPIEKFDEFVEKWNKSGGEEVTKRAREWY 521 L A ++ IIG+ + FD+ VE W KSGG E+ K + Y Sbjct: 458 ILDAR--TQFIIGEIDEKGFDDAVELWKKSGGNELMKDLNKLY 498 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 98.6 bits (244), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 128/513 (24%), Positives = 215/513 (41%), Gaps = 92/513 (17%) Query: 73 KEKLGVGVINPYVEWNGGTNYLNQLNLKIAANE-MPDLF-VPQQGLEDSLAKNGAIADLT 130 +E+LG+ + + + N + QL L +++ E +PD+F V ++ L +G + + Sbjct: 71 EEELGIKFVTEWTQPND-EQFNTQLRLMMSSGEKLPDVFQVNDTAIKADLLSSGLVMPVD 129 Query: 131 ELIPEYAP----NVWAAIPEDIWKVVKANDPTGQGKIYYIPNVVEYGRYAG--------L 178 E I YA ++ P+ + K G Y IP R++G Sbjct: 130 EAIETYASPRLKQLFEQFPQAFYPSTK------DGVRYGIP------RFSGGNGSDTLLW 177 Query: 179 IRQDWLDKLGLSMPKTQDEYVKVLEAFRDQDPNGNGQKDEIPT-----------GGREQA 227 IRQDWLDK L P T +E +++ F ++DPN G+ D I G + Sbjct: 178 IRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTIGITFAAGDYGFRGGNIADS 237 Query: 228 RWMDHLFAMYGVAMFEGYPEWDIYDGELTYSAVTPNMKAALEFISKLYKEGLIDKE-SLL 286 W +F +G + + + D DG L Y ++ P+MK L I + Y++G + + ++L Sbjct: 238 SW---VFGAFGDFVPGAWSKGD--DGSLVYGSIQPSMKDGLAKIHEWYEKGYLATDFAIL 292 Query: 287 NSKDKWDGKIQSDKVG-----NYFHWVEQGNLIFENLYKNTGVKAEFSVLPIPEVEGYKG 341 ++ D + I S + G + H G + V + V P P V G G Sbjct: 293 SAGDAQESFI-SGRSGIMAAPPWAHGYPIGEM--------AQVVPDAIVKPFPHVSGPNG 343 Query: 342 FYTMKKIASPLWVVKNNKDEEKLMATLKLLNNMY----DKSNWKDLFLGVEGMHYTMKDG 397 + + NKD E + + + +Y +S++ + L EG Y M+DG Sbjct: 344 QAGRRGEGYVVGSFLFNKDFEHMDKFFEYWDAIYGYTFGESDYFEYGL-FEGYDYVMEDG 402 Query: 398 KAVKLPDDKSTQE---NQILNPA-----TSVSTLDF----TIDLFNSTVSE--------- 436 + V D +E ++++P T+V T+ F ++ F+ T E Sbjct: 403 EPVY--DHAQFEEITGEEVVDPGRYFLPTNVPTIPFEMYSLLEEFHETGREPETGYEAGL 460 Query: 437 ---DSKWAIDQVIRNMQEAQQYAKVIAGDGMPASVYDGYPDINNRTLYAEYASKIIIGQY 493 D + I N Q + G P V + + E II G Sbjct: 461 AGRDPDYIEAAAIVNRQNDLRIINEFNGPPTPTMVSRSE---HLERMEDELFVDIIYGNK 517 Query: 494 PIEKFDEFVEKWNKSGGEEVTKRAREWYDKVQQ 526 P++ FD+FVE+W +SGGE +T+ EWY+ VQQ Sbjct: 518 PLDAFDQFVEEWRQSGGEAITEEVNEWYESVQQ 550 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 81/321 (25%), Positives = 145/321 (45%), Gaps = 39/321 (12%) Query: 95 NQLNLKIAANEMPDLFVPQQGLEDSLA---KNGAIADLTELIPEYAPNVWAAIPEDIWKV 151 +++N +A+ +P + Q S+ ++G ++ + E+ PN+ + + E I + Sbjct: 94 DRVNAALASGNLPKVITLQDFRTSSVLNAFRSGQFWEIGPYLDEF-PNL-SEMNETILEN 151 Query: 152 VKANDPTGQGKIYYIPNVVEYGRYAGLIRQDWLDKLGLSMPKTQDEYVKVLEAFRDQDPN 211 V + GKIY I R +IRQDWL+ LGL MP +E +V AF DPN Sbjct: 152 VSVD-----GKIYGIYRERPLSRQGVVIRQDWLENLGLEMPTNLEELYEVARAFTYDDPN 206 Query: 212 GNGQKDEIPTGGREQARWMDHLFAMY-GVAMFEGYP-EWDIYD-GELTYSAVTPNMKAAL 268 G+G D + R D +F + +A + G P EW D G+L +T + Sbjct: 207 GSGADDTVGFTDRN-----DLIFGAFKTLASYHGTPNEWGFDDEGKLMPEFMTDEYVETM 261 Query: 269 EFISKLYKEGLIDKESLLNSKDKWDGKIQSDKVGNYF-HWVEQGNLIFENLYKNTGVKAE 327 ++ LY EGLI+ E + SK++ + + G Y + V+ NL + K+ + E Sbjct: 262 NYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYVGNMVDARNL--RDKAKDINPEME 319 Query: 328 FSV---LPIPEVE-------GYKGFYTMKKIASPLWVVKNNKDEEKLMATLKLLNNMYDK 377 ++ + P+ E G+ G + + K + + E +L L + + ++ Sbjct: 320 VAITNRIAGPDGEDRVWAQGGHNGIFAIPKSSV--------ETEAELKRILAFFDRIAEE 371 Query: 378 SNWKDLFLGVEGMHYTMKDGK 398 + + G+E +HY DG+ Sbjct: 372 DVYNLMAYGIEDVHYETIDGE 392 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 62.0 bits (149), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 97/413 (23%), Positives = 175/413 (42%), Gaps = 40/413 (9%) Query: 22 GCSG-NKNNAEGLAAENQNQASGGDKVIDLEVWGTNIGYQPVTKGSKLYELYKEKLGVGV 80 GC G ++++AEG A G D + L W + +Q K + E+ EKL Sbjct: 13 GCKGTDQSSAEGKA--------GPDSKVKLS-WMAILYHQQPPKDRAIKEI--EKLTNTE 61 Query: 81 INPYVEWNGGTNYLNQLNLKIAANEMPDLFVPQQGLEDSLA---KNGAIADLTELIPEYA 137 ++ + W ++LN +AA +P + Q S+ ++G ++ + I +Y Sbjct: 62 LD--ITWVPDAVKEDRLNAALAAGNLPQIVTIQDIKNSSVMNAFRSGMFWEIGDYIKDY- 118 Query: 138 PNVWAAIPEDIWKVVKANDPTGQGKIYYIPNVVEYGRYAGLIRQDWLDKLGLSMPKTQDE 197 PN+ + K++ N T GK+Y I R +IR+DWLD L L PKT DE Sbjct: 119 PNL-----NKMNKLINKN-VTIDGKLYGIYRERPLSRQGIVIRKDWLDNLNLKTPKTLDE 172 Query: 198 YVKVLEAFRDQDPNGNGQKDEIPTGGREQARWMDHLFAMY-GVAMFEGYP-EWDIYDGEL 255 +V +AF + DP+ +G+ D R D ++ + + +EG P +W G+ Sbjct: 173 LYEVAKAFTEDDPDKDGKDDTFGLADRN-----DLIYGAFKTIGSYEGMPTDWKESGGKF 227 Query: 256 TYSAVTPNMKAALEFISKLYKEGLIDKESLLNSKDKWDGKIQSDKVGNYFHWVEQGNLIF 315 T +T K + ++ KL G ++K+ + SK + K G Y GN++ Sbjct: 228 TPDFMTQEYKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFSQGKAGIYI-----GNMVD 282 Query: 316 ENLYKNTGVKAEFSVLPIPEVEGYKGFYTMKKIASPLWVVKNNKDEEKLMATLKLLNNMY 375 ++ + I ++G G + V K K A LK + + Sbjct: 283 AVNLRDHASDKSMKLEIINRIKGPDGKERVWASGGHNGVFAFPKTSVKTEAELKRILAFF 342 Query: 376 DKSNWKDLF----LGVEGMHYTMKDGKAVKLPDDKSTQENQILNPATSVSTLD 424 D+ +D++ G++G+HY + K + + + P +++ +D Sbjct: 343 DRIAEEDVYSLMTYGIDGVHYNKGEDKTFTRKESQVKDWQTDIQPLSALIAID 395 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,702,879 Number of extensions: 1902932 Number of successful extensions: 2508 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2499 Number of HSP's successfully gapped: 7 Length of query: 526 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 415 Effective length of database: 25,576,357 Effective search space: 10614188155 Effective search space used: 10614188155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 118 (50.1 bits)