BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0024.p (289 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_lplC [G] COG0395 ABC-type sugar transport system, permease co... 193 2e-49 BH0481 [G] COG0395 ABC-type sugar transport system, permease com... 191 1e-48 BS_ytcP [G] COG0395 ABC-type sugar transport system, permease co... 186 3e-47 BH1066 [G] COG0395 ABC-type sugar transport system, permease com... 159 7e-39 SP0091 [G] COG0395 ABC-type sugar transport system, permease com... 146 4e-35 >BS_lplC [G] COG0395 ABC-type sugar transport system, permease component Length = 295 Score = 193 bits (491), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 94/240 (39%), Positives = 152/240 (63%) Query: 50 LWPKQFTLDSWKYIVDRPDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVI 109 ++PK FTLD++KY+ P +S +++ T++GT + L T AYPL+K + +++ Sbjct: 56 IFPKTFTLDAYKYVFSTPTFLRSMGVSIFITVVGTAVQLFFTFTMAYPLAKRHVKGRNLL 115 Query: 110 MLAVVIAMIFKAPIVPYFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEE 169 + V+ +M+F ++P +L V+ +GL D LI+P +NPFNLII++ FF+Q +ELEE Sbjct: 116 LNLVIFSMLFSGGMIPTYLVVKSLGLLDTYWALILPMAINPFNLIIIKNFFQQLPRELEE 175 Query: 170 AAFLEGCGYFRMLFQFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIR 229 +A ++GC + ++ LPLSK V+AT ALFY V +WN F H L ++ D+ +PLQ+ +R Sbjct: 176 SAKIDGCSEIGVFWRIALPLSKPVIATFALFYAVGIWNDFFHALLYINDSAKWPLQMVLR 235 Query: 230 QFITDDSVIMAGAASTAHLNYNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVKG 289 Q + + + E+ ++ A ++ A +PI+ VYPFLQK+F KG +IGSVKG Sbjct: 236 QVTILSDLTATNGDTMQNAVPPEQGIKLAVIVIATLPILAVYPFLQKHFAKGMLIGSVKG 295 >BH0481 [G] COG0395 ABC-type sugar transport system, permease component Length = 293 Score = 191 bits (484), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 101/289 (34%), Positives = 166/289 (57%), Gaps = 4/289 (1%) Query: 4 SRGEKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYI 63 S G +VF L LFS+I +P L+P F+LD+++YI Sbjct: 6 SLGSRVFNIFNYLFLFLFSMICFLPFVHIVAGSFASVEEVLQKRFILFPTTFSLDAYRYI 65 Query: 64 VDRPDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFKAPI 123 +++S +++ T++GT ++ +T+L AY LS+ + V+M VV M+F + Sbjct: 66 FSTDIIFRSLIISILVTVVGTGFSMFLTSLMAYGLSRKDLDGRRVLMFLVVFTMLFSGGM 125 Query: 124 VPYFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGYFRMLF 183 +P FL VR +GL D LI+P +N FNLII++ FF+ + LEE+A ++GC + F Sbjct: 126 IPTFLVVRSLGLLDTYWALIIPTAINAFNLIILKNFFQNIPESLEESAKIDGCNDVGIFF 185 Query: 184 QFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMAGAA 243 + V PLS +AT++LFY V WN F + +L D++ +P+Q+ +RQ + S M G Sbjct: 186 RIVFPLSMPAIATISLFYAVTYWNTFMQAILYLNDSSMWPIQVLLRQIVIMSSG-MQGDL 244 Query: 244 STAHLNY---NERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVKG 289 + + E+T++ A ++ A +P+++VYPF+QK+F KGA++GSVKG Sbjct: 245 TELGAGFVMPPEQTIKMAVIVVATIPVLIVYPFIQKHFAKGALLGSVKG 293 >BS_ytcP [G] COG0395 ABC-type sugar transport system, permease component Length = 286 Score = 186 bits (472), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 95/284 (33%), Positives = 167/284 (58%), Gaps = 1/284 (0%) Query: 7 EKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYIVDR 66 ++F +I G L++F++I ++P L P F+LD+++YI Sbjct: 3 NRLFDMLIYGFLLMFALICVLPFIHVIAASFATVEEVVSKKFILIPTTFSLDAYRYIFST 62 Query: 67 PDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFKAPIVPY 126 ++KS +++ T+IGT +++ +++L AY LS+ + +M VV M+F ++P Sbjct: 63 DIIYKSLLVSVFVTVIGTAVSMFLSSLMAYGLSRRDLIGRQPLMFLVVFTMLFSGGMIPT 122 Query: 127 FLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGYFRMLFQFV 186 FL V+ +GL D+ LI+P +N FNLII++ FF+ LEE+A ++GC + F+ V Sbjct: 123 FLVVKSLGLLDSYWALILPTAINAFNLIILKNFFQNIPSSLEESAKIDGCNDLGIFFKIV 182 Query: 187 LPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMAGAASTA 246 LPLS +AT++LFY V WN + + +L D+ +P+Q+ +RQ + S + + Sbjct: 183 LPLSLPAIATISLFYAVTYWNTYMTAILYLNDSAKWPIQVLLRQIVIVSSGMQGDMSEMG 242 Query: 247 HLN-YNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVKG 289 + E+T++ A ++ A +P+++VYPF+QK+F KGA++GSVKG Sbjct: 243 SGSPPPEQTIKMAVIVVATIPVLLVYPFIQKHFAKGALLGSVKG 286 >BH1066 [G] COG0395 ABC-type sugar transport system, permease component Length = 293 Score = 159 bits (401), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 91/288 (31%), Positives = 159/288 (55%), Gaps = 4/288 (1%) Query: 4 SRGEKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYI 63 ++G KVF L+LF+++ ++PL NLV P FTLD+++ Sbjct: 6 TKGYKVFTAFNYTFLLLFAVLCILPLVHIFAVSLSGSGAAAANLVMFLPVDFTLDAYERT 65 Query: 64 VDRPDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPE--FRWGSVIMLAVVIAMIFKA 121 + + +SF +++ ++GTV+++ AY LSK + F+ + VI M+F Sbjct: 66 IANDNFIRSFLISVVRVVLGTVISMGAMLCAAYALSKEDRDFKGRKFYIWFFVITMLFNG 125 Query: 122 PIVPYFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQ-FSKELEEAAFLEGCGYFR 180 +VP ++ V G+GL + L++P +N FNLI++ FF+ K LEE+AF++G +F+ Sbjct: 126 GLVPTYMVVTGLGLTNTIWALVLPPAINAFNLILLLNFFRTAVPKSLEESAFIDGANHFQ 185 Query: 181 MLFQFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMA 240 + + LP+S +AT++LF V WN + + + D +PLQ ++ + Sbjct: 186 IFLKIYLPISVPAIATVSLFTMVFHWNSWFDGMIYNNDARNYPLQTFLQTIVVQQDFSQI 245 Query: 241 GAASTAHLNYNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVK 288 A T N ++RT++AA + A +P+++VYPF+QKYFVKG ++G+ K Sbjct: 246 TDAETLR-NLSQRTVQAAQIFIAALPMLIVYPFIQKYFVKGIVLGAEK 292 >SP0091 [G] COG0395 ABC-type sugar transport system, permease component Length = 307 Score = 146 bits (368), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 92/290 (31%), Positives = 155/290 (53%), Gaps = 8/290 (2%) Query: 4 SRGEKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYI 63 S+ +F II+GL+ L I+ P + +P QF LD ++++ Sbjct: 20 SKKADIFFSIISGLIALSCIL---PFVFVIIISVTDEKSLLQYGYSFFPSQFGLDGFEFL 76 Query: 64 VDRPD-LWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFKAP 122 D + ++ F+++ T++GT+ + +T +AY +S+ F++ + V+++M+F A Sbjct: 77 AQFKDKILQALFISVFVTVVGTLTNVFITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAG 136 Query: 123 IVP-YFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKE-LEEAAFLEGCGYFR 180 +VP Y + R + L D LIVP +L+PFN+I+MR+FFK+ E + E+A ++G R Sbjct: 137 LVPGYIMVTRVLQLGDTVWALIVPMLLSPFNIILMRSFFKKTIPEAILESARIDGASEAR 196 Query: 181 MLFQFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMA 240 + FQ LPLS +AT+ L + WN + + L +++ N +PLQ + Q + I Sbjct: 197 IFFQICLPLSLPGIATITLLTALGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAK 256 Query: 241 GAASTAHLNYN--ERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVK 288 L + T R A V+ A +PI ++YPF Q+YFVKG IG VK Sbjct: 257 AVGLIGQLGVALPKETGRMAMVVVATLPIAILYPFFQRYFVKGLTIGGVK 306 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.332 0.145 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,774,116 Number of extensions: 659748 Number of successful extensions: 2489 Number of sequences better than 1.0e-05: 142 Number of HSP's gapped: 2333 Number of HSP's successfully gapped: 142 Length of query: 289 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 184 Effective length of database: 26,268,361 Effective search space: 4833378424 Effective search space used: 4833378424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 115 (48.9 bits)