BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0021.p (527 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1123 [T] COG4753 Response regulator containing CheY-like recei... 183 8e-46 BH2109 [T] COG4753 Response regulator containing CheY-like recei... 171 3e-42 BH3842 [T] COG4753 Response regulator containing CheY-like recei... 159 1e-38 BH1910 [T] COG4753 Response regulator containing CheY-like recei... 131 2e-30 lin0157 [K] COG2207 AraC-type DNA-binding domain-containing prot... 96 2e-19 >BH1123 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 526 Score = 183 bits (464), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 138/541 (25%), Positives = 269/541 (49%), Gaps = 45/541 (8%) Query: 2 RLVLIDDEKGIVEGLKKIIGRYLPECKVVGEAYNGLEGFELIQRLRPDIVITDIRMPVAD 61 ++V+IDDE+ +V GLKKII C+ VGEA NG G ++++ P IV+TDI MP+ Sbjct: 3 KVVIIDDEEHVVRGLKKIISWKALGCECVGEAANGRRGLDIVRETSPHIVLTDIYMPIMY 62 Query: 62 GLDMIKMLEDNKIQTKFILLSGYADFEYARRGMKLGVQFYINKPVEEEELHDSVNRVMET 121 GLDMI++L ++ + ++LSGY++FE+AR+ M+L + Y++KP + + + RV+ Sbjct: 63 GLDMIQILREDGFDGEVVILSGYSEFEHARKAMQLNITDYLSKPASVDTIETVLKRVINK 122 Query: 122 IRADRSKLQRLNELKHEV---HSRMQEETLRDIL-------DLGNEHTSLVEELLHSAQI 171 + + + L EL ++V ++ E ++ +L ++ E + + + I Sbjct: 123 LEEKQRQQGYLQELTNKVKRYEPLVETEWMKSLLTGRLRNGEIPEEFLAKITKWKDQQHI 182 Query: 172 PTAGTWFNSIIIEIEGSTELLKEIGFQPLLQHIDQTLRNYYKVYRFRYF---GPQIAIVI 228 + T+ E++ + L F+ + + + + N Y + FRY + A+ + Sbjct: 183 ACSLTYDGPQNKELKPADWYL----FRYSVHKMIREVLNGYPI-DFRYLEMDAYRSALCL 237 Query: 229 AHNSN----IVYGRLLHVIQGLRQAVYQKLNLSISVGIGTVYKRGTGICQSFEEA-RNAL 283 N + ++ L+ + + Q +Y N+ +V G V + T + +SF EA + Sbjct: 238 HVNKSVSEPVLSCMLMEIKDQVEQGIYSSFNVKTTVHYGRVKEDWTDLSESFHEACMESQ 297 Query: 284 SYKLIKEGNPVISFTEIKKITEHSHPVPEELITRLEDGLDNMDEEECVAVIREMFR--GM 341 S+ L GNP T P E +L D + D V+ E G Sbjct: 298 SFPL---GNPYEDGTRA--------TFPMESSQKLYDAIRYADATRAKEVLEEAVGVLGE 346 Query: 342 AAESGMNPADLQLRCLNILLSSIRKLSF---QQLQQNELVGRHILSLEGFSRFRTLEYLE 398 S + + + I+ S+ + + ++ + +H+ + + + +L Sbjct: 347 VPYSTLVGYKVGIEIWTIITYSLYDIGILIQEMFDEDFDLAQHLTKQGNWQEW--IRFLC 404 Query: 399 EWMIQVIRGIINFKVEHNLSQKKDIISEIKLYVSKHFNEQISLADLAARFFISPYYLSQL 458 E Q+ + + + N+ K+ +I ++ +Y+ KH E I+L D+A +IS YL Q+ Sbjct: 405 E---QIEHICYHQQWDENIKHKQ-LIEQVMIYIEKHLAENITLQDIADELYISRNYLGQI 460 Query: 459 FKRKTGDNYLNFLTQMRIDKAKELLENTDLKVYEICRMVGYLDAQHFSRMFEKLAGCKPR 518 FK+ G+++ N++T++R++KAK++++ + +YE+ VGY++ +F+ MF+K G PR Sbjct: 461 FKKVVGESFKNYVTRLRMEKAKKMIQEGNFLIYEVSEKVGYVNPAYFTTMFKKYTGYTPR 520 Query: 519 E 519 + Sbjct: 521 D 521 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 171 bits (433), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 135/551 (24%), Positives = 269/551 (48%), Gaps = 58/551 (10%) Query: 2 RLVLIDDEKGIVEGLKKIIGRYLPECKVVGEAYNGLEGFELIQRLRPDIVITDIRMPVAD 61 +++L DDE + GLK +I +V EA NG + F+L+Q ++PD+VITDIRMPV D Sbjct: 3 KVLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPVFD 62 Query: 62 GLDMI-KMLEDNKIQTKFILLSGYADFEYARRGMKLGVQFYINKPVEEEELHDSVNRVME 120 GL +I K+ E TKFI++SGY DF YA++ ++ G ++ KP++++EL ++ ++ Sbjct: 63 GLQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKLAA 122 Query: 121 TI------RADRSKLQRLNELKHEVHSRMQEETLRDI---LDLGNEHTSLVEELLHSAQI 171 T+ +D+ K+ H + +E +R L++ N H Sbjct: 123 TLDQEKATNSDQEKVLLDQIFIHSLGENKKENEVRRWAAKLNINNRH------------- 169 Query: 172 PTAGTWFNSIIIEIEGSTELLKEIGF------QPLLQHIDQTLRNYYKVYRFRYFGPQIA 225 ++ +++E+ L + GF +P+ + I +T+ + Q+ Sbjct: 170 -----FYYYLLLEVNN---LSLDKGFINEQYKEPIRKVIRETVARQAATEEYTAIYEQLD 221 Query: 226 -----IVIAHNSNIVYGRLLHVIQGLRQAVYQKLNLSISVGIGTVYKRGTGICQSFEEAR 280 ++ + + G++ + + LR+ + N+ ++V +G + I +S+E A Sbjct: 222 HAYGLLITSEHLKKFNGQIEPLAEKLRKDIQDFTNIPLTVYVGAKTDDLSTIKKSYETAN 281 Query: 281 NALSYKLIKEGNPVISFTEIKKITEHSHPVPEELITRLEDGLDNMDEEECVAVIREMFRG 340 + +YK I V + + + + L L + ++ + + V +I ++F Sbjct: 282 SLRNYKYIFNEKNVFIHEQTESVDTTYDELNNHLFVTLMEQMEENNSDRIVNIIDKIFSE 341 Query: 341 MAAESGMNPADLQL---RCLNILLSSIRKLSFQQLQQNELVGRHILSLEGFSRFRTLEYL 397 + + P+ ++ RC++ ++ +++ + G+ I+S + + + + Sbjct: 342 FQTKKFV-PSAVKTSVNRCVHKVMQTVQAMEGD-------TGK-IISAQAMTNWESYNLT 392 Query: 398 EEWMIQVI-RGIINFKV---EHNLSQKKDIISEIKLYVSKHFNEQISLADLAARFFISPY 453 + + QV + ++ V E K I ++K Y+ H++E ISL +A+ F+++P Sbjct: 393 FDQLKQVFTKFVLEAAVIIQELRKENMKSDIYKVKTYIENHYHENISLKSIASLFYMNPV 452 Query: 454 YLSQLFKRKTGDNYLNFLTQMRIDKAKELLENTDLKVYEICRMVGYLDAQHFSRMFEKLA 513 Y+ QLFK+ G + +L ++R+++AK+LL TD++VYE+ VG+ +F FEK+ Sbjct: 453 YMGQLFKKTFGVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTDYFVTQFEKIN 512 Query: 514 GCKPREYRKNL 524 P EYR L Sbjct: 513 KMTPTEYRNKL 523 >BH3842 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 530 Score = 159 bits (401), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 144/555 (25%), Positives = 260/555 (46%), Gaps = 70/555 (12%) Query: 1 VRLVLIDDEKGIVEGLKKIIGRYLPECKVVGEAYNGLEGFELIQRLRPDIVITDIRMPVA 60 + ++++DDE+ EGLK + + P +VGEA NG + E +PD+++ DI+MP Sbjct: 2 INVLIVDDEQIEREGLKLFL-KDRPNIHIVGEASNGRQAIEKALETQPDLMMMDIKMPGV 60 Query: 61 DGLDMIKMLEDNKIQTKFILLSGYADFEYARRGMKLGVQFYINKPVEEEELHDSVNRV-- 118 DG+ +K ++ +T+FI++S + FEYAR M+ GV+ Y+ KP +E++ +++R+ Sbjct: 61 DGVQAVKEIKQQLPETEFIMVSAFDTFEYAREVMRYGVKEYLLKPTTKEDVLTALDRIVG 120 Query: 119 -METIRADRSKLQRLNELKHEVHSRMQEETLRDILDLGNEHTSLVEELLHSAQIPTAGTW 177 +E R +++ +L + ++ S +Q E+ IL L +H E + W Sbjct: 121 EIEQAREEKNDQYKLQQRLEQISSFVQTES---ILYLLMDHIQDYEWM----------EW 167 Query: 178 FNSIIIEIEGSTELLKEIGFQPLLQHIDQTLRNYYKVYRFRYFGPQIA------------ 225 + E EG + F + I +T + + + G Q+ Sbjct: 168 NEWLRTEQEGYAVVF---SFHQQGKEIRKTEKQQWHARIKKEIGLQLPKSTVGPFIGHHL 224 Query: 226 --IVIAHNSNIVYGR-----LLHVIQGLRQAVYQKLNLSISVGIGTVYKRGTGICQSFEE 278 +++ N N + + ++ ++ ++QAV + G+G +Y R S+ E Sbjct: 225 PILMLTENENGMTKQEREQVVIQLLHKVKQAVQPA---KVKTGVGGIY-RLHFFSTSYRE 280 Query: 279 ARNALSYKLIKEGNPVISFTEIKKITEHSHPVPEELITRLEDGLDNMDEEECVAVIREMF 338 A AL + + + KI +P E +L + + + D E+ + Sbjct: 281 ALLALDIIYDHKQASYYFYDKNVKIRADEQ-LPIERENKLIEAIKSGDGEKALQAFDHYV 339 Query: 339 RGMAAESGMNPADLQLRCLNILLSSIRKLSFQQLQQ---------NELVGRHILSLEGFS 389 + + LRC L +RK LQQ NE+ G H + + F+ Sbjct: 340 QAI------------LRCSKHPLHDMRKY----LQQLFIILGRLLNEM-GLHWETAQDFA 382 Query: 390 RFRTLEYLEEWMIQVIRGIINFKVEHNLSQKKDIISEIKLYVSKHFNEQISLADLAARFF 449 RF T+ + E I I+ E + ++ ++ + YV HF E ++L DLA Sbjct: 383 RFSTVHQVTEHGKSEILHIVRTLQEWRTTDEQSLMLLARDYVHDHFREPLTLDDLAKHIG 442 Query: 450 ISPYYLSQLFKRKTGDNYLNFLTQMRIDKAKELLENTDLKVYEICRMVGYLDAQHFSRMF 509 +SPYY+S+LFK + + ++TQ+R++ AK+LL T + EI +GY D +FSR+F Sbjct: 443 LSPYYVSKLFKERFDLTFSEYVTQLRVEHAKQLLSLTQKSLKEITYEIGYKDPNYFSRVF 502 Query: 510 EKLAGCKPREYRKNL 524 +KL G P +YRK++ Sbjct: 503 KKLTGKSPSDYRKSI 517 >BH1910 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 506 Score = 131 bits (330), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 132/539 (24%), Positives = 240/539 (44%), Gaps = 65/539 (12%) Query: 3 LVLIDDEKGIVEGLKKIIGRYLPECKVVGEAYNGLEGFELIQRLRPDIVITDIRMPVADG 62 ++++DDE + EGLK +I +V+ A NG EGF ++L PD++I DIRMP DG Sbjct: 4 VMIVDDEAMVREGLKALISWEEYGFRVIDTARNGKEGFTKYRQLSPDLMIVDIRMPEVDG 63 Query: 63 LDMIKMLEDNKIQTKFILLSGYADFEYARRGMKLGVQFYINKPVEEEELHDSVNRVMETI 122 L +I+ + FI+LSG++DF YA++ + Y+ KP++E+EL DS+ +V I Sbjct: 64 LSLIESIRAEDSAIHFIILSGHSDFNYAKKALGCQCDDYLLKPLDEDELIDSLKKVYGRI 123 Query: 123 RADRSKLQRLNELKHEVHSRMQEETLRDILD--------LGNEHTSLVEELLHSAQIPTA 174 ++ +++EL V + E L ++L+ +G EH + Q+ Sbjct: 124 ----TRHHQVHELVERVQEKQVEMVLTELLEGTPLSEIPIGKEHNFFATDY----QVALV 175 Query: 175 GTWFNSIIIEIEGSTELLKEIGFQPLLQHIDQTLRNYY-----KVYRFRYFGPQIAIVIA 229 + + +++ E +E P ++I +N + K +R+ F + Sbjct: 176 ENRQDRLTKQLKRLIEEQEEGYVIPFPEYIVIVCKNRFLQKRGKDHRYHLFAKWLE---- 231 Query: 230 HNSNIVYGRLLHVIQGLRQAVYQKLNLSISVGIGTVYKRGTGICQSFEEARNALSYKLIK 289 LH G S V + +Y +S++ A+ K Sbjct: 232 -------EEELHACAG-----------SKVVNLADIY-------ESYQSAKRLYEKKFFV 266 Query: 290 EGNPVISFTEIKKITEHSHP--VPEELITRLEDGLDNMDEEECVAVIREM----FRGMAA 343 E + + ++ ++ P E RL +D + E CVA+I E+ + Sbjct: 267 EKGRLFTADQLVDRSKPEAPEFSLESYADRLYYSIDIGNMEGCVAIIEELKNVVIVHLQE 326 Query: 344 ESGMNPADLQLRCLNILLSSIRKLSFQQLQQNELVGRHILSLEGFSRFRTLEYLEEWMIQ 403 E + A QL NIL +R + LV ++ +F L + ++ + Sbjct: 327 EKEIKVALFQLYS-NILHQLVRSHPNMEGVLEPLV----MNAGDIYQFEHLPGIMAFLTK 381 Query: 404 VIRGIINFKVEHNLSQKKDIISEIKLYVSKHFNEQISLADLAARFFISPYYLSQLFKRKT 463 ++ +I+ + Q ++ ++ + K + + + L LA+ F + YL +LFK T Sbjct: 382 QLKYVIDCM---DNGQPDLLLRKMIALIHKDYAKNLRLETLASVFNYNRAYLGKLFKNYT 438 Query: 464 GDNYLNFLTQMRIDKAKELLENTDLKVYEICRMVGYLDAQHFSRMFEKLAGCKPREYRK 522 G+ + +L ++RI+ K LL+ KVYE+ +GY + +F R F+K G P +RK Sbjct: 439 GEYFNTYLDKVRIENGKRLLQKG-YKVYEVAEKIGYANVDYFHRKFKKYVGVSPSTFRK 496 >lin0157 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 277 Score = 95.5 bits (236), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 46/113 (40%), Positives = 79/113 (69%), Gaps = 1/113 (0%) Query: 412 KVEHNLSQKKDIISEIKLYVSKHFNEQISLADLAARFFISPYYLSQLFKRKTGDNYLNFL 471 K+ + QK+ +++EI Y+ KH E +SL+ L+ +ISP Y+S++FK +TG++ +N+L Sbjct: 164 KLSTDEQQKQMLVNEIIHYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYL 223 Query: 472 TQMRIDKAKELLENTDLKVYEICRMVGYLDAQHFSRMFEKLAGCKPRE-YRKN 523 ++R+ +A+ELL+N D+ V + MVGY DA +FS++F+K G P E +RK+ Sbjct: 224 IKIRLTRAEELLKNKDVTVKQAANMVGYNDAYYFSKLFKKYYGFPPSENWRKS 276 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,018,778 Number of extensions: 1576095 Number of successful extensions: 9899 Number of sequences better than 1.0e-05: 282 Number of HSP's gapped: 9973 Number of HSP's successfully gapped: 294 Length of query: 527 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 416 Effective length of database: 25,576,357 Effective search space: 10639764512 Effective search space used: 10639764512 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)