BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0010.p (318 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2515 [T] COG0642 Signal transduction histidine kinase 100 3e-21 BH1154_2 [T] COG0642 Signal transduction histidine kinase 86 5e-17 baeS [T] COG0642 Signal transduction histidine kinase 72 1e-12 ZbaeS [T] COG0642 Signal transduction histidine kinase 72 1e-12 >lin2515 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 100 bits (249), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 0/180 (0%), Positives = 0/180 (0%), Gaps = 1/180 (0%) Query: 115 PEMSSMEQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHDIKTPLTSVIGYLSLLHEAP 174 Sbjct: 130 189 Query: 175 DMPNEQKENYTKITLEKAYHLERLINDFFEVERYNQQTIQLKKQNVDLSYMLIQMPDEFY 234 Sbjct: 190 249 Query: 235 PSLTPAGKRMIVNVE-DTITVYGDSEKLARVFNNILKNAIAYSDDNSTIEVSAKVQKDMA 293 Sbjct: 250 309 >BH1154_2 [T] COG0642 Signal transduction histidine kinase Length = 274 Score = 86.3 bits (212), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 52/176 (29%), Positives = 108/176 (61%), Gaps = 4/176 (2%) Query: 117 MSSMEQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHDIKTPLTSVIGYLSLLHEAPDM 176 ++ + Q + +K+ +++ E E SE+ K +L+ ++HD++TPLTS+I Y LL +A + Sbjct: 28 LALLAQNINTMKKGMKQSEREQAKSERLKTELITNVSHDLRTPLTSIITYTELLKDADE- 86 Query: 177 PNEQKENYTKITLEKAYHLERLINDFFEVERYNQQTIQLKKQNVDLSYMLIQMPDEFYPS 236 +++ +Y +I KA L+ LI+D FE + + I+L + VD+ +L Q E+ + Sbjct: 87 --QERADYIEIIDRKARRLKILIDDLFEASKMSSGNIELTLETVDVLQLLQQALAEYGEA 144 Query: 237 LTPAGKRMIVNV-EDTITVYGDSEKLARVFNNILKNAIAYSDDNSTIEVSAKVQKD 291 L + ++ ++V E+ + + D +K+ RVF+N+++N + YS +++ + +S K +K+ Sbjct: 145 LEQSSLQLRISVPEEPLYIKADGQKIWRVFDNLIQNILKYSLEHTRVYLSVKEKKE 200 >baeS [T] COG0642 Signal transduction histidine kinase Length = 467 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 47/196 (23%), Positives = 99/196 (50%), Gaps = 15/196 (7%) Query: 100 GIDALITDEADIKLSP----EMSSMEQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHD 155 G L + +++P E+ + Q + TL+K ++Q +RD + ++H+ Sbjct: 199 GTHKLAAGDFTTRVTPTSEDELGKLAQDFNQLASTLEK-------NQQMRRDFMADISHE 251 Query: 156 IKTPLTSVIGYLSLLHEAPDMPNEQKENYTKITLEKAYHLERLINDFFEVERYNQQTIQL 215 ++TPL + G L + + + E + E L +L++D ++ ++ + Sbjct: 252 LRTPLAVLRGELEAIQDG--VRKFTPETVASLQAEVGT-LTKLVDDLHQLSMSDEGALAY 308 Query: 216 KKQNVDLSYMLIQMPDEFYPSLTPAGKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAY 275 +K VDL +L F G ++ ++ D+ITV+GD ++L ++FNN+L+N++ Y Sbjct: 309 QKAPVDLIPLLEVAGGAFRERFASRGLKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRY 368 Query: 276 SDDNSTIEVSAKVQKD 291 +D ++++SA Q+D Sbjct: 369 TDSGGSLQISAG-QRD 383 >ZbaeS [T] COG0642 Signal transduction histidine kinase Length = 467 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 47/196 (23%), Positives = 99/196 (50%), Gaps = 15/196 (7%) Query: 100 GIDALITDEADIKLSP----EMSSMEQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHD 155 G L + +++P E+ + Q + TL+K ++Q +RD + ++H+ Sbjct: 199 GTHKLAAGDFTTRVTPTSEDELGKLAQDFNQLASTLEK-------NQQMRRDFMADISHE 251 Query: 156 IKTPLTSVIGYLSLLHEAPDMPNEQKENYTKITLEKAYHLERLINDFFEVERYNQQTIQL 215 ++TPL + G L + + + E + E L +L++D ++ ++ + Sbjct: 252 LRTPLAVLRGELEAIQDG--VRKFTPETVASLQAEVGT-LTKLVDDLHQLSMSDEGALAY 308 Query: 216 KKQNVDLSYMLIQMPDEFYPSLTPAGKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAY 275 +K VDL +L F G ++ ++ D+ITV+GD ++L ++FNN+L+N++ Y Sbjct: 309 QKAPVDLIPLLEVAGGAFRERFASRGLKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRY 368 Query: 276 SDDNSTIEVSAKVQKD 291 +D ++++SA+ Q D Sbjct: 369 TDSGGSLKISAE-QHD 383 Score = 71.2 bits (173), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 45/192 (23%), Positives = 97/192 (50%), Gaps = 14/192 (7%) Query: 100 GIDALITDEADIKLSP----EMSSMEQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHD 155 G L + +++P E+ + Q + TL+K ++Q +RD + ++H+ Sbjct: 199 GTHKLAAGDFTTRVTPTSEDELGKLAQDFNQLASTLEK-------NQQMRRDFMADISHE 251 Query: 156 IKTPLTSVIGYLSLLHEAPDMPNEQKENYTKITLEKAYHLERLINDFFEVERYNQQTIQL 215 ++TPL + G L + + + E + E L +L++D ++ ++ + Sbjct: 252 LRTPLAVLRGELEAIQDG--VRKFTPETVASLQAEVGT-LTKLVDDLHQLSMSDEGALAY 308 Query: 216 KKQNVDLSYMLIQMPDEFYPSLTPAGKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAY 275 +K VDL +L F G ++ ++ D+ITV+GD ++L ++FNN+L+N++ Y Sbjct: 309 QKAPVDLIPLLEVAGGAFRERFASRGLKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRY 368 Query: 276 SDDNSTIEVSAK 287 +D ++++SA+ Sbjct: 369 TDSGGSLKISAE 380 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,218,239 Number of extensions: 670558 Number of successful extensions: 1795 Number of sequences better than 1.0e-05: 84 Number of HSP's gapped: 1817 Number of HSP's successfully gapped: 84 Length of query: 318 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 212 Effective length of database: 26,153,027 Effective search space: 5544441724 Effective search space used: 5544441724 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)