BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0009.p (223 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhfP [CR] COG0604 NADPH:quinone reductase and related Zn-depe... 176 3e-44 SA2160 [CR] COG0604 NADPH:quinone reductase and related Zn-depen... 166 2e-41 lin0767 [CR] COG0604 NADPH:quinone reductase and related Zn-depe... 166 2e-41 SAP008 [CR] COG0604 NADPH:quinone reductase and related Zn-depen... 155 6e-38 YPO3663 [CR] COG0604 NADPH:quinone reductase and related Zn-depe... 154 8e-38 >BS_yhfP [CR] COG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductases Length = 346 Score = 176 bits (446), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 5/150 (3%), Positives = 24/150 (16%), Gaps = 1/150 (0%) Query: 1 MTETFKALVVDK-GETFSVAVQPLSLEKLPAGEVLIRVAYSSVNYKDGLASIPNGNIVRN 59 M + + + A + +A + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 YPFIPGIDLSGTVVSSADPRFHEGQAVIATGYGIGVSHYGGFSEYARIPAGWVMPLPAGL 119 + + + + ++ R + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 120 SLREAMIYGTAGFTAAMSIQALEAHGAAPD 149 + + + + + + D Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTD 150 >SA2160 [CR] COG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductases Length = 346 Score = 166 bits (421), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 10/147 (6%), Positives = 23/147 (15%), Gaps = 2/147 (1%) Query: 1 MTETFKALVVDKGETFSVA--VQPLSLEKLPAGEVLIRVAYSSVNYKDGLASIPNGNIVR 58 M + T + + A L +N D P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 59 NYPFIPGIDLSGTVVSSADPRFHEGQAVIATGYGIGVSHYGGFSEYARIPAGWVMPLPAG 118 + + + V + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 119 LSLREAMIYGTAGFTAAMSIQALEAHG 145 L +Y + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYS 147 >lin0767 [CR] COG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductases Length = 346 Score = 166 bits (421), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 9/148 (6%), Positives = 23/148 (15%), Gaps = 1/148 (0%) Query: 3 ETFKALVVDK-GETFSVAVQPLSLEKLPAGEVLIRVAYSSVNYKDGLASIPNGNIVRNYP 61 + + T + + A + + R Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 62 FIPGIDLSGTVVSSADPRFHEGQAVIATGYGIGVSHYGGFSEYARIPAGWVMPLPAGLSL 121 G ++ R A +G + + L Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 122 REAMIYGTAGFTAAMSIQALEAHGAAPD 149 + + G + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWT 149 >SAP008 [CR] COG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductases Length = 346 Score = 155 bits (391), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 12/142 (8%), Positives = 30/142 (21%), Gaps = 1/142 (0%) Query: 5 FKALVVDK-GETFSVAVQPLSLEKLPAGEVLIRVAYSSVNYKDGLASIPNGNIVRNYPFI 63 + E ++ + ++ + + +V L + V P Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 64 PGIDLSGTVVSSADPRFHEGQAVIATGYGIGVSHYGGFSEYARIPAGWVMPLPAGLSLRE 123 G+ + A E + + G ++ G + + R Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 124 AMIYGTAGFTAAMSIQALEAHG 145 Y Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGK 143 >YPO3663 [CR] COG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductases Length = 346 Score = 154 bits (390), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 7/145 (4%), Positives = 20/145 (13%), Gaps = 1/145 (0%) Query: 5 FKALVVDKGETFSVA-VQPLSLEKLPAGEVLIRVAYSSVNYKDGLASIPNGNIVRNYPFI 63 + + E + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 64 PGIDLSGTVVSSADPRFHEGQAVIATGYGIGVSHYGGFSEYARIPAGWVMPLPAGLSLRE 123 V + + +IA+ + + + SL+ Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 124 AMIYGTAGFTAAMSIQALEAHGAAP 148 + + G Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLG 145 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,479,617 Number of extensions: 499556 Number of successful extensions: 1696 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 1708 Number of HSP's successfully gapped: 24 Length of query: 223 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 121 Effective length of database: 26,614,363 Effective search space: 3220337923 Effective search space used: 3220337923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)