BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0007.p (463 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0399 [C] COG0281 Malic enzyme 376 e-104 MA1735 [C] COG0281 Malic enzyme 357 2e-98 BH3168 [C] COG0281 Malic enzyme 357 2e-98 BS_ytsJ [C] COG0281 Malic enzyme 347 2e-95 CC3549_1 [C] COG0281 Malic enzyme 318 8e-87 >BH0399 [C] COG0281 Malic enzyme Length = 397 Score = 376 bits (966), Expect = e-104, Method: Compositional matrix adjust. Identities = 193/368 (52%), Positives = 250/368 (67%), Gaps = 1/368 (0%) Query: 83 GKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPGRAYSLTMKRNTVAVVTDGTAVLG 142 GK+EI K+ I + +DLSLVYTPGVA VC IAE P + SLT + N VA+VTDGTAVLG Sbjct: 19 GKLEIVGKMEITDEKDLSLVYTPGVADVCKQIAEQPDKVNSLTSRGNMVAIVTDGTAVLG 78 Query: 143 LGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKDPEEIIRVVKAVTAGLGGINLEDI 202 LG+IGP+AAMPVMEGKA+LFK+ A+ID FP+CL T+D +EI++++KA++ GINLEDI Sbjct: 79 LGNIGPKAAMPVMEGKALLFKKFANIDGFPVCLNTQDTDEIVQIIKALSPTFAGINLEDI 138 Query: 203 SSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXXXXXXXXGKRLEDCRXXXXXXXXX 262 ++PRCFEIE RL ELDIPVFHDDQHGT K + + Sbjct: 139 AAPRCFEIEERLKEELDIPVFHDDQHGTAIVVLAALTNALKVVNKLNQPIKMVINGAGAA 198 Query: 263 XXSICRLLLAAGARKVYAVDRAGILHKGQTYDNAEWSWLAEATNPEGLTGGLTEAMQGAD 322 +I LLL AG + + V G++ KG+ + N + +AE TN G+ G L +A+ AD Sbjct: 199 GTAIANLLLHAGFKDITLVSLEGVVAKGEAWLNPMQAKMAEVTNLAGVKGTLKDAITDAD 258 Query: 323 VFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEPELA-ESYARVVATGRSDYPNQIN 381 VFIGVS G + ++H+++MA+D IV A+ANP PEI PE A E+ A VV TGRSDYPNQ+N Sbjct: 259 VFIGVSGPGAMKKEHIQAMAKDPIVLALANPIPEIYPEEAIEAGAAVVGTGRSDYPNQVN 318 Query: 382 NVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHPDELNEQYIIPSIFNEKVVEGVR 441 N+L FPGIFRGALD AK + +EMK+AAA AIA V+ E+N IIP + +V + V Sbjct: 319 NLLAFPGIFRGALDAGAKTITMEMKIAAAYAIADVLSDKEVNANMIIPDALDARVAQRVA 378 Query: 442 EAVIRAAI 449 EAV A+ Sbjct: 379 EAVATTAL 386 >MA1735 [C] COG0281 Malic enzyme Length = 390 Score = 357 bits (917), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 195/381 (51%), Positives = 254/381 (66%), Gaps = 4/381 (1%) Query: 80 HIGGKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPGRAYSLTMKRNTVAVVTDGTA 139 +GG +E+ K+ ++ DLS+ YTPGVA C I E+P Y T+K+NTVAVVTDG+A Sbjct: 4 RLGGVLEVESKVRLRTIHDLSVAYTPGVAEPCRKIKENPSLVYLYTIKKNTVAVVTDGSA 63 Query: 140 VLGLGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKDPEEIIRVVKAVTAGLGGINL 199 VLGLG+IGP AA+PVMEGKA++FK+ A IDAFP+CL T+D EE+I+ VK + GGINL Sbjct: 64 VLGLGNIGPCAALPVMEGKAIIFKEFAGIDAFPICLNTQDTEEVIKAVKYLAPAFGGINL 123 Query: 200 EDISSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXXXXXXXXGKRLEDCRXXXXXX 259 EDIS+PRCFEIE RL ELDIPV HDDQHG K L + + Sbjct: 124 EDISAPRCFEIESRLREELDIPVIHDDQHGAAIVVFAGLLNALKIVKKELGELKILITGL 183 Query: 260 XXXXXSICRLLLAAGA--RKVYAVDRAGILHKGQTYD-NAEWSWLAEATNPEGLTGGLTE 316 +I R L+ AGA K+ D GI+++G+ D N + +A TNP L GGL E Sbjct: 184 GAAGVAIFRFLVRAGADPAKILTCDSQGIVYEGREKDMNPIKAEIATLTNPGKLKGGLKE 243 Query: 317 AMQGADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEPELAE-SYARVVATGRSD 375 A G D+FIGVS GGI++E+ + SMA D IV AMANPTPEI P+ A+ + AR+VATGRSD Sbjct: 244 AFPGTDLFIGVSVGGIVTEEMISSMANDAIVMAMANPTPEIMPDAAKRAGARIVATGRSD 303 Query: 376 YPNQINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHPDELNEQYIIPSIFNEK 435 +PNQ+NN L FPG+F+GAL A+ + EM++AAA A+A+VV EL+E YIIP ++ Sbjct: 304 FPNQLNNCLSFPGVFKGALGTCARKITPEMEMAAAHALANVVSVGELSEDYIIPEPLDKH 363 Query: 436 VVEGVREAVIRAAIATGMARR 456 VV V +AV A++ + ARR Sbjct: 364 VVPAVAKAVANASLESCAARR 384 >BH3168 [C] COG0281 Malic enzyme Length = 410 Score = 357 bits (916), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 185/376 (49%), Positives = 251/376 (66%), Gaps = 2/376 (0%) Query: 83 GKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPGRAYSLTMKRNTVAVVTDGTAVLG 142 GK+E K+P++N DLSL Y+PGVA C I ED Y TMK N VAVV++GTAVLG Sbjct: 17 GKLESKSKVPVRNARDLSLAYSPGVAEPCKEIFEDVETVYEYTMKGNMVAVVSNGTAVLG 76 Query: 143 LGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKDPEEIIRVVKAVTAGLGGINLEDI 202 LG+IGPEA++PVMEGKA+LFK A +DAFP+CL T D ++I+ VK + GG+NLEDI Sbjct: 77 LGNIGPEASLPVMEGKAVLFKSFAGVDAFPICLRTTDVDQIVETVKLLEPTFGGVNLEDI 136 Query: 203 SSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXXXXXXXXGKRLEDCRXXXXXXXXX 262 ++P CFEIE RL E +IPVFHDDQHGT GKR+ + + Sbjct: 137 AAPSCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLINALKLTGKRMSEIKVVANGAGAA 196 Query: 263 XXSICRLLLAAGARKVYAVDRAGILHKGQTYD-NAEWSWLAEATNPEGLTGGLTEAMQGA 321 +I +L+ G R + D G ++ G+ Y N + +A+ TN + + GGL + ++GA Sbjct: 197 GIAIIKLMQRMGVRDIIMCDSKGAIYDGRPYGMNDMKAEVAKWTNRDKIDGGLGDVLKGA 256 Query: 322 DVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEPELAESY-ARVVATGRSDYPNQI 380 DVFIGVS G L+++ +++M +D I+FAMANP PEI PE A++ A+V+ TGRSD+PNQ+ Sbjct: 257 DVFIGVSVAGALTKEMIENMNDDPIIFAMANPVPEIMPEEAKAAGAKVIGTGRSDFPNQV 316 Query: 381 NNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHPDELNEQYIIPSIFNEKVVEGV 440 NNVL FPGIFRGALD RA +N EMK+AA AIA +V ++L+ Y+IP+ F+ +V V Sbjct: 317 NNVLAFPGIFRGALDARATHINEEMKVAAVHAIADLVAEEDLDPDYVIPAPFDPRVAPAV 376 Query: 441 REAVIRAAIATGMARR 456 AV + A+ TG+ARR Sbjct: 377 AAAVAKTAMETGVARR 392 >BS_ytsJ [C] COG0281 Malic enzyme Length = 410 Score = 347 bits (891), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 189/375 (50%), Positives = 248/375 (66%), Gaps = 2/375 (0%) Query: 83 GKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPGRAYSLTMKRNTVAVVTDGTAVLG 142 GK+E K+ ++N +DLSL Y+PGVA C I ED + Y TMK N VAVVTDGTAVLG Sbjct: 16 GKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKGNMVAVVTDGTAVLG 75 Query: 143 LGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKDPEEIIRVVKAVTAGLGGINLEDI 202 LG+IGPEAA+PVMEGKA+LFK A +DAFP+ L T D ++I+ VK + GG+NLEDI Sbjct: 76 LGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLLEPTFGGVNLEDI 135 Query: 203 SSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXXXXXXXXGKRLEDCRXXXXXXXXX 262 ++P CF IE RL E +IPVFHDDQHGT GK + + Sbjct: 136 AAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAA 195 Query: 263 XXSICRLLLAAGARKVYAVDRAGILHKGQTYD-NAEWSWLAEATNPEGLTGGLTEAMQGA 321 +I +LL G R + D G +++G+ N + +A+ TN + G L + + A Sbjct: 196 GIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTNQDRKDGSLKDVIVDA 255 Query: 322 DVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEPELA-ESYARVVATGRSDYPNQI 380 DVFIGVS G L+++ ++SMA+D I+FAMANP PEI PE A E+ A VV TGRSD+PNQ+ Sbjct: 256 DVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAGASVVGTGRSDFPNQV 315 Query: 381 NNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHPDELNEQYIIPSIFNEKVVEGV 440 NNVL FPGIFRGALD RA +N EMK+AA +AIAS+V DEL+ Y+IP+ F+++V V Sbjct: 316 NNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSADYVIPAPFDKRVAPAV 375 Query: 441 REAVIRAAIATGMAR 455 +AV +AA+ TG+AR Sbjct: 376 AKAVAKAAMETGVAR 390 >CC3549_1 [C] COG0281 Malic enzyme Length = 453 Score = 318 bits (816), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 181/392 (46%), Positives = 237/392 (60%), Gaps = 21/392 (5%) Query: 83 GKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPGRAYSLTMKRNTVAVVTDGTAVLG 142 GKI I P P+ + DLSL Y+PGVA AIA DP AY T K N VAV+++GTA+LG Sbjct: 29 GKIAIVPTKPMATQRDLSLAYSPGVAVPVHAIAADPDMAYEYTSKGNLVAVISNGTAILG 88 Query: 143 LGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKDPEEIIRVVKAVTAGLGGINLEDI 202 LGD+G A+ PVMEGK++LFK+ D+D+ + + TKDP+EII VVK + GGINLEDI Sbjct: 89 LGDLGALASKPVMEGKSVLFKRFGDVDSIDIEVTTKDPDEIITVVKNIGVTFGGINLEDI 148 Query: 203 SSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXXXXXXXXGKRLEDCRXXXXXXXXX 262 SP CF IE L LDIPVFHDDQHGT GK++ED + Sbjct: 149 KSPECFRIETELQELLDIPVFHDDQHGTAIICAAGLINACHITGKKIEDVKVVLNGPGAA 208 Query: 263 XXSICRLLLAAGAR--KVYAVDRAGILHKGQTYDNAEWSWLAEATNPEGLTGGLTEAMQG 320 + L+ A G R AVD G+L++G+T +W A A + T L EA+ G Sbjct: 209 GIASLELIKAMGVRAENCIAVDSKGVLYRGRTEGMNQWKS-AHAVETDKRT--LAEAVVG 265 Query: 321 ADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEPELAESYAR--VVATGRSDYPN 378 ADV +G+S+ G + + + SMA + ++FAMANP PEI PE + + ++ TGRSDYPN Sbjct: 266 ADVLLGLSAKGAFTPEMIASMAPNPVIFAMANPDPEITPEEVKRVRKDAIMGTGRSDYPN 325 Query: 379 QINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVH---PDE-----------LNE 424 Q+NNVL FP IFRGALD RA+ VN EMK+A AQA+A + PDE Sbjct: 326 QVNNVLGFPYIFRGALDVRARRVNHEMKIACAQALAMLAREDVPDEVAAAYHGRQLKFGP 385 Query: 425 QYIIPSIFNEKVVEGVREAVIRAAIATGMARR 456 YIIPS F+ +++ V V +AA+ TG+AR+ Sbjct: 386 DYIIPSAFDPRLIWYVPPFVAQAAMDTGVARK 417 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,538,605 Number of extensions: 1283914 Number of successful extensions: 2927 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 2853 Number of HSP's successfully gapped: 28 Length of query: 463 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 353 Effective length of database: 25,691,691 Effective search space: 9069166923 Effective search space used: 9069166923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)