BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0027.p (431 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0487 [R] COG0673 Predicted dehydrogenases and related proteins 267 3e-71 BS_yteT [R] COG0673 Predicted dehydrogenases and related proteins 245 1e-64 ZycjS [R] COG0673 Predicted dehydrogenases and related proteins 84 4e-16 ECs1894 [R] COG0673 Predicted dehydrogenases and related proteins 84 4e-16 ycjS [R] COG0673 Predicted dehydrogenases and related proteins 84 5e-16 >BH0487 [R] COG0673 Predicted dehydrogenases and related proteins Length = 430 Score = 267 bits (682), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 156/445 (35%), Positives = 239/445 (53%), Gaps = 41/445 (9%) Query: 7 KKYVIVGTGGRA-EFFYGALVTVYNETSELAAFCDVNQTRMNFANHMLTDKYGYHEVPTY 65 KKY I G RA F ++ Y + ++ A D + R+ A V Y Sbjct: 2 KKYAICGVSNRALRMFIAPILDQYAKNHQVVALLDQDPRRLEIAKEQYPQ---LASVRGY 58 Query: 66 KAEDFDLMIEETRPDFVLVTTIDRTHHKYIIRAMELGCDVISEKPMTIDEEKCQQIFDAI 125 + F+ M+ ET+PD +LV + D TH YI++ + VI+EKPM + +++ +A Sbjct: 59 DDQSFEQMMNETKPDVLLVCSRDDTHIDYILKGLAHDLHVITEKPMVTTAKDAKRVIEAE 118 Query: 126 ERTGKNLRVTFNYRYAPHNTKIREIIMDGVLGEILSVNFEWLLNTQHGADYFRRWHRDKR 185 ++ + VTFNYRY H+ KI+E+I++G LG I S++F W ++T HGA YF+RW+R + Sbjct: 119 NKSRGKVTVTFNYRYGAHHRKIKELILEGRLGRITSIDFNWYIDTYHGASYFKRWNRVRD 178 Query: 186 NSGGLLVHKSTHHFDLMNFWLASRPDTVFAMGDLRFYGKE--------------NAEKRG 231 SGGL +HKSTHHFDL+N+W+ RP FA G L +YG + +++ Sbjct: 179 YSGGLSIHKSTHHFDLVNWWVDQRPIEAFAYGALNYYGADGELNPSKKNGRFCGTCDEQD 238 Query: 232 ITEFYQR---AYGSKAAENDPFALHLDRNEHLKSMYLDAEHEDGYQRDQSVFGDNISIED 288 ++Y+R S AA++D H++ +Y D Y+ D +F + I+IED Sbjct: 239 RCQYYRRWTTRSQSSAAKDD----HIEYVPSTAKLYTD------YRPDACIFDEEINIED 288 Query: 289 TMNVMVKYKNKTVMNYSLNAYMPWEGFIIVFNGTEGRMEVR-ITEQSYVNSGGKKENEGA 347 T V+Y T+++YS+N P+EG+ +V NGT+GR+E + E S + Sbjct: 289 TYVATVRYDRGTLLSYSINFSTPYEGYHLVINGTKGRIETKEYHEPSRIPF--------P 340 Query: 348 LEGVNITIYPHFKEPYRVQFESSAGGHGGGDPVLLRDIFDKKQEDR-FGRAASHKDGAMS 406 + I YP F + ++ GGHGGGDP+LL D+F R + A + GA S Sbjct: 341 FDEQTIDYYPLFGSKETIHVVTNEGGHGGGDPILLEDLFLGVDPARPYEILAGAQAGADS 400 Query: 407 IMTGIAANRSMRTGQPVKVDDLWKM 431 I G A RS++TGQP+K+DDL K+ Sbjct: 401 IAVGEAVWRSVQTGQPIKIDDLLKV 425 >BS_yteT [R] COG0673 Predicted dehydrogenases and related proteins Length = 428 Score = 245 bits (626), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 150/445 (33%), Positives = 229/445 (51%), Gaps = 41/445 (9%) Query: 7 KKYVIVGTGGRA-EFFYGALVTVYNETSELAAFCDVNQTRMNFANHMLTDKYGYHEVPTY 65 K V G RA F L+ ++ E+ D + R + VP + Sbjct: 2 KNIVFCGLSSRAFSMFIKPLMERFSTHYEITGLLDADPKRFAVCKKKFPE---LAHVPEF 58 Query: 66 KAEDFDLMIEETRPDFVLVTTIDRTHHKYIIRAMELGCDVISEKPMTIDEEKCQQIFDAI 125 + FD M+ ++PD V+V D TH YI+++++ DVI+EKPM + ++ +A Sbjct: 59 SEDAFDEMMRVSKPDIVIVAGRDDTHVAYIVKSLQWNTDVITEKPMVTTVQDANRVLEAE 118 Query: 126 ERTGKNLRVTFNYRYAPHNTKIREIIMDGVLGEILSVNFEWLLNTQHGADYFRRWHRDKR 185 ++ + V FNYRY+P + KI+E+I+DG +G + SV+ W ++T HGA YF+RW+R ++ Sbjct: 119 AKSEGKVTVAFNYRYSPFHRKIKEMILDGKIGRVTSVDLNWYIDTYHGASYFKRWNRSRQ 178 Query: 186 NSGGLLVHKSTHHFDLMNFWLASRPDTVFAMGDLRFYGKEN-----AEKRG--------- 231 SGGL VHKSTHHFDL+N+WL P+ VFA G L +YG ++ E+ G Sbjct: 179 FSGGLSVHKSTHHFDLVNWWLGQNPEEVFAYGALNYYGPDSEWNPLPEEDGRFCGTCRVK 238 Query: 232 -ITEFYQRAY--GSKAAENDPFALHLDRNEHLKSMYLDAEHEDGYQRDQSVFGDNISIED 288 +Y R + SKA+ D HL+ + S+Y Y+ D +F + I IED Sbjct: 239 EKCHYYSRWHPRSSKASIKDD---HLEAGDQ-SSLYT------AYRPDACIFDEEIDIED 288 Query: 289 TMNVMVKYKNKTVMNYSLNAYMPWEGFIIVFNGTEGRMEV-RITEQSYVNSGGKKENEGA 347 T VKY +++YS+ P+EG+ + NGT+GR+E E S + A Sbjct: 289 TYVAAVKYDGGALLSYSIIFSAPYEGYRLTINGTKGRIESNEFHEPSRIPF--------A 340 Query: 348 LEGVNITIYPHFKEPYRVQFESSAGGHGGGDPVLLRDIF-DKKQEDRFGRAASHKDGAMS 406 I YP F+ +Q + GGHGGGDP+LL D+F K R+ A + GA S Sbjct: 341 FPEQTIEYYPLFESKQTIQVVKNEGGHGGGDPLLLEDLFLGKDPLRRYDILAGAEAGAYS 400 Query: 407 IMTGIAANRSMRTGQPVKVDDLWKM 431 I G RS+ +P+ + +L++M Sbjct: 401 IAVGEGMWRSVAEKKPIGMKELFQM 425 >ZycjS [R] COG0673 Predicted dehydrogenases and related proteins Length = 351 Score = 84.0 bits (206), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 58/202 (28%), Positives = 97/202 (48%), Gaps = 11/202 (5%) Query: 5 SKKKYVIVGTGGRAEFFYGALVTVYNETSELAAFCDVNQTRMNFANHMLTDKYGYHEVPT 64 S + I+G G A+ + + N+ EL A CD +R++ A L +KYG V Sbjct: 8 SPLRVAIIGAGQVADKVHASYYCTRNDL-ELVAVCD---SRLSQA-QALAEKYGNASV-- 60 Query: 65 YKAEDFDLMIEETRPDFVLVTTIDRTHHKYIIRAMELGCDVISEKPMTIDEEKCQQIFDA 124 +D M+ +PD V V + +R H+++ + A+E GC V+ EKP + E+ +++ D Sbjct: 61 --WDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCEKPPAMTPEQAREMCDT 118 Query: 125 IERTGKNLRVTFNYRYAPHNTKIREIIMDGVLGEILSVNFEWLLNTQHGADYFRRWHRDK 184 + GK L F++R+A ++RE + +GVLGEI L G + + + Sbjct: 119 ARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARALRRC--GVPGWGVFTNKE 176 Query: 185 RNSGGLLVHKSTHHFDLMNFWL 206 GG L+ H D + L Sbjct: 177 LQGGGPLIDIGIHMLDAAMYVL 198 >ECs1894 [R] COG0673 Predicted dehydrogenases and related proteins Length = 351 Score = 84.0 bits (206), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 58/202 (28%), Positives = 97/202 (48%), Gaps = 11/202 (5%) Query: 5 SKKKYVIVGTGGRAEFFYGALVTVYNETSELAAFCDVNQTRMNFANHMLTDKYGYHEVPT 64 S + I+G G A+ + + N+ EL A CD +R++ A L +KYG V Sbjct: 8 SPLRVAIIGAGQVADKVHASYYCTRNDL-ELVAVCD---SRLSQA-QALAEKYGNASV-- 60 Query: 65 YKAEDFDLMIEETRPDFVLVTTIDRTHHKYIIRAMELGCDVISEKPMTIDEEKCQQIFDA 124 +D M+ +PD V V + +R H+++ + A+E GC V+ EKP + E+ +++ D Sbjct: 61 --WDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCEKPPAMTPEQAREMCDT 118 Query: 125 IERTGKNLRVTFNYRYAPHNTKIREIIMDGVLGEILSVNFEWLLNTQHGADYFRRWHRDK 184 + GK L F++R+A ++RE + +GVLGEI L G + + + Sbjct: 119 ARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARALRRC--GVPGWGVFTNKE 176 Query: 185 RNSGGLLVHKSTHHFDLMNFWL 206 GG L+ H D + L Sbjct: 177 LQGGGPLIDIGIHMLDAAMYVL 198 >ycjS [R] COG0673 Predicted dehydrogenases and related proteins Length = 351 Score = 83.6 bits (205), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 58/202 (28%), Positives = 97/202 (48%), Gaps = 11/202 (5%) Query: 5 SKKKYVIVGTGGRAEFFYGALVTVYNETSELAAFCDVNQTRMNFANHMLTDKYGYHEVPT 64 S + I+G G A+ + + N+ EL A CD +R++ A L +KYG V Sbjct: 8 SPLRVAIIGAGQVADKVHASYYCTRNDL-ELVAVCD---SRLSQA-QALAEKYGNASV-- 60 Query: 65 YKAEDFDLMIEETRPDFVLVTTIDRTHHKYIIRAMELGCDVISEKPMTIDEEKCQQIFDA 124 +D M+ +PD V V + +R H+++ + A+E GC V+ EKP + E+ +++ D Sbjct: 61 --WDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCEKPPAMTPEQAREMCDT 118 Query: 125 IERTGKNLRVTFNYRYAPHNTKIREIIMDGVLGEILSVNFEWLLNTQHGADYFRRWHRDK 184 + GK L F++R+A ++RE + +GVLGEI L G + + + Sbjct: 119 ARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARALRRC--GVPGWGVFTNKE 176 Query: 185 RNSGGLLVHKSTHHFDLMNFWL 206 GG L+ H D + L Sbjct: 177 LQGGGPLIDIGIHMLDAAMYVL 198 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,594,509 Number of extensions: 1403450 Number of successful extensions: 4820 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 4799 Number of HSP's successfully gapped: 59 Length of query: 431 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 322 Effective length of database: 25,807,025 Effective search space: 8309862050 Effective search space used: 8309862050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)