BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0026.p (164 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0832 [K] COG1695 Predicted transcriptional regulators 59 2e-09 >CAC0832 [K] COG1695 Predicted transcriptional regulators Length = 240 Score = 59.3 bits (142), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 44/141 (31%), Positives = 74/141 (52%), Gaps = 5/141 (3%) Query: 3 GYEIQQKMISAQTDKWAYVQPASIYHALRKLDKDELVRLEKLEQTGNRSKAIYSITSKGK 62 GY+IQ+ + D+WA V+ SIY+AL KL+KD + + E+TG R + IY+I+ KG Sbjct: 17 GYDIQRFIEIDGMDQWAKVKSGSIYYALNKLEKDGFIFTLREERTGARIRKIYAISDKGV 76 Query: 63 HEFNSMLISSFKKASVVFPSALYTVLTFMDEAPKEEIIGALEVQQQAIQTIYDEMKAGQK 122 E +L K + + + + ++EII ++ +Q IQ++ K + Sbjct: 77 EELRRVLKEELLKPIDNVEADKFMIYLMFNRLERDEII---DLTRQHIQSLEQRKKWWED 133 Query: 123 -EKEKYSEVSKNV-LLIFENM 141 K K SE + V +L F+N+ Sbjct: 134 GRKIKVSEATLKVEILHFDNV 154 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.128 0.342 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 9,622,726 Number of extensions: 297206 Number of successful extensions: 618 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 621 Number of HSP's successfully gapped: 1 Length of query: 164 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 66 Effective length of database: 27,075,699 Effective search space: 1786996134 Effective search space used: 1786996134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 111 (47.4 bits)