BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0025.p (401 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1281.04 [C] COG0667 Predicted oxidoreductases (related to ar... 89 2e-17 SPCC1281.06c_2 [CI] COG5274 Cytochrome b involved in lipid metab... 89 2e-17 L129379 [GEPR] COG0477 Permeases of the major facilitator superf... 83 6e-16 BS_yceE [T] COG2310 Uncharacterized proteins involved in stress ... 72 2e-12 DR0472 [GEPR] COG0477 Permeases of the major facilitator superfa... 72 2e-12 >SPCC1281.04 [C] COG0667 Predicted oxidoreductases (related to aryl-alcohol dehydrogenases) Length = 346 Score = 88.6 bits (218), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 0/297 (0%), Positives = 0/297 (0%), Gaps = 19/297 (6%) Query: 1 MNT-ITPTKQKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQT----EIGLLFGS- 54 ++A V+ D +V I Y+ + + Sbjct: 171 GKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVEL 230 Query: 55 YAIALFIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXX 114 A + + G + G Sbjct: 231 LAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQ 290 Query: 115 ITWTSGLAILADLYPSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVL 174 + + + + M + + L + Sbjct: 291 -MALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEA- 348 Query: 175 AIADGVLRIFLLRDL--PERKSGTF-------LSSFGLLRNR-SLLLISGVVIIGSAIPS 224 Sbjct: 349 408 Query: 225 VLEPTLPLHLQEKFNAEPGFIGM-LFAIPTLSYALATPWIGKLSSRIGYKKAVLIGL 280 Sbjct: 409 465 >SPCC1281.06c_2 [CI] COG5274 Cytochrome b involved in lipid metabolism Length = 123 Score = 88.6 bits (218), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 0/297 (0%), Positives = 0/297 (0%), Gaps = 19/297 (6%) Query: 1 MNT-ITPTKQKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQT----EIGLLFGS- 54 Sbjct: 171 230 Query: 55 YAIALFIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXX 114 Sbjct: 231 290 Query: 115 ITWTSGLAILADLYPSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVL 174 Sbjct: 291 -- 348 Query: 175 AIADGVLRIFLLRDL--PERKSGTF-------LSSFGLLRNR-SLLLISGVVIIGSAIPS 224 Sbjct: 349 408 Query: 225 VLEPTLPLHLQEKFNAEPGFIGM-LFAIPTLSYALATPWIGKLSSRIGYKKAVLIGL 280 Sbjct: 409 465 >L129379 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 83.2 bits (204), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 91/394 (23%), Positives = 177/394 (44%), Gaps = 20/394 (5%) Query: 10 KSALFVVGIAIFLDMLIYGLVVPILPKYASALGA-SQTEI----GLLFGSYAIALFIATP 64 + L V+ F+ + L+ P + Y S LG S+TE+ GL+F S I L I +P Sbjct: 6 QRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVLAIVSP 65 Query: 65 VFGALSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAIL 124 ++G L+D+ GR+ +L W ++ +A++ Sbjct: 66 LWGRLADQKGRKLMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFV-SNSMALM 124 Query: 125 ADLYPSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIF 184 A P E+ G + L +G AG L+GP +GG + + GY+ F ++ L +F Sbjct: 125 ASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVNITGYRRVFSVTGIIMFLVFFLALF 184 Query: 185 LLRDL--PERKSG--TFLSSFGLLRNRSLLL-ISGVVIIGSAIPSVLEPTLPLHLQEKFN 239 ++++ P K ++ + ++ + +++ + +I + P L L++QE + Sbjct: 185 FVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQELMH 244 Query: 240 AEPGFI---GMLFAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSM 296 + G++ A P + +A P++G+L ++G +K + GL I ++A+ ++ Sbjct: 245 GQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITAMTTNV 304 Query: 297 WMQAASLVLLGISMGMVLAPCLPALADISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSG 356 W +L+G+S +L LA S Q+ G F+ +A S G GP+ Sbjct: 305 WFLIVMRLLVGVSDAAILPSVQAILAKESPQA---ITGRIFSYNQSAQSIGAFAGPLLGS 361 Query: 357 VLTDALGLKLSYLSVGCAALLYMFIMLPSFRRTE 390 + + + Y+ VG +A+L +F ++ F T+ Sbjct: 362 AIAGFIDYR--YVFVG-SAILVVFNLINYFTHTK 392 >BS_yceE [T] COG2310 Uncharacterized proteins involved in stress response, homologs of TerZ and putative cAMP-binding protein CABP1 Length = 408 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 96/407 (23%), Positives = 164/407 (40%), Gaps = 21/407 (5%) Query: 5 TPTKQKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTEI-----GLLFGSYAIAL 59 TP K L V + FL + LV+P LP Y LG + G++F + Sbjct: 8 TPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSITFLFS 67 Query: 60 FIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTS 119 IA+P +G L+DR GR+ +L + W + Sbjct: 68 AIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNA 127 Query: 120 GLAILADLYPSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADG 179 A++A P + G A+G +G +G LLGP GG + G + F A + I Sbjct: 128 N-ALIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITASVLILCF 186 Query: 180 VLRIFLLRD----LPERKSGTFLSSFGLLRNRSLL--LISGVVIIGSAIPSVLEPTLPLH 233 + +F +R+ + +++ L+N L+ L +II A S+ P L L+ Sbjct: 187 FVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSI-APILTLY 245 Query: 234 LQEKFN--AEPGFI-GMLFAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLS 290 ++E + FI GM+ ++P ++ L+ P +GKL RIG +K ++ L + L Sbjct: 246 VRELAGNVSNVAFISGMIASVPGVAALLSAPRLGKLGDRIGPEKILITALIFSVLLL--- 302 Query: 291 ALPDSMWMQAASLVLLGISMGMVLAPCLPALAD-ISEQSGLPSYGITFAIYNTAYSFGMI 349 +P S L +L +G LPA+ + S G F+ + G + Sbjct: 303 -IPMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNV 361 Query: 350 VGPVTSGVLTDALGLKLSYLSVGCAALLYMFIMLPSFRRTEVTRLNS 396 GP+ ++ G + +L L S RR + ++++ Sbjct: 362 TGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLRRRRIPQVSN 408 >DR0472 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 407 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 99/373 (26%), Positives = 161/373 (43%), Gaps = 43/373 (11%) Query: 6 PTKQKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTE----IGLLFGSYAIALFI 61 P + + LF++ + +D++ GL++P+LP L S+ IG+L +YA FI Sbjct: 5 PRRPPALLFIL-LTALIDVMGIGLIIPVLPGLVKELAGSEAGGARFIGVLTAAYAAMQFI 63 Query: 62 ATPVFGALSDRIGRRGPMLWXXXXXXXXXXXXXXXEE-YWMXXXXXXXXXXXXXITWTSG 120 P+ G LSDR GRR +L +W+ +T + Sbjct: 64 FAPILGTLSDRFGRRPVLLLSLAGMVLDYLLLFFSPNLWWLLVGRLIAGVTGASLTVAN- 122 Query: 121 LAILADLYPSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGV 180 A +AD+ E+R K G + G +LGP LGGW+ + G + PFLFAA L + + Sbjct: 123 -AYIADVTAPEDRAKNFGRLGATWGVGFILGPMLGGWLGE-HGLRAPFLFAAGLTALNFL 180 Query: 181 LRIFLL-RDLPERKSGT-------------FLSSFGLLRNRSLLLISGVVIIGSAIPSVL 226 + +L LP K G L+ + LLR +L V++G A V+ Sbjct: 181 YGLLVLPESLPPEKRGAAGGGTLNPFTPLRALTEYPLLRGLTLTF----VLLGLA-GQVI 235 Query: 227 EPTLPLHLQEKFNAEPGFIGMLFAIPTLSYA-----LATPWIGKLSSRIGYKKAVLIGLA 281 T L+ + P G+ FA+ L A L +P+I +R G ++ ++ GL Sbjct: 236 VSTWVLYTEGVLRWSPLQNGIAFAVYGLLTAGVQAGLISPFI----ARFGERRTIMTGL- 290 Query: 282 VVAISLPLSALPDSMWMQAASLVLLGISMGMVLAPCLPALAD-ISEQSGLPSYGITFAIY 340 VA +L L + ++A ++ L + +G + PA+ IS Q G G Sbjct: 291 -VASTLEFVVLSVA---RSAPVLYLSLVVGALGGLAQPAMQGLISRQVGEQEQGRVMGAI 346 Query: 341 NTAYSFGMIVGPV 353 + S + GP+ Sbjct: 347 TSLNSLVGVFGPL 359 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,952,778 Number of extensions: 1111326 Number of successful extensions: 4649 Number of sequences better than 1.0e-05: 89 Number of HSP's gapped: 4582 Number of HSP's successfully gapped: 103 Length of query: 401 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 292 Effective length of database: 25,807,025 Effective search space: 7535651300 Effective search space used: 7535651300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)