BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0024.p (463 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM2013 [V] COG0534 Na+-driven multidrug efflux pump 192 8e-49 yeeO [V] COG0534 Na+-driven multidrug efflux pump 188 2e-47 YPO1571 [V] COG0534 Na+-driven multidrug efflux pump 166 5e-41 CAC1611 [V] COG0534 Na+-driven multidrug efflux pump 154 4e-37 ECs2782 [V] COG0534 Na+-driven multidrug efflux pump 113 7e-25 >STM2013 [V] COG0534 Na+-driven multidrug efflux pump Length = 485 Score = 192 bits (489), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 136/441 (30%), Positives = 228/441 (51%), Gaps = 7/441 (1%) Query: 26 YRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXNMIDSLNIFLISVFIAVSTG 85 +R+I L +PI ++ V+ + V++T + DS N+ +++ F A+ G Sbjct: 39 WREITPLAIPIFLENTCVLLMGVLSTFLVSWLGKEAMAGVGLADSFNMVIMAFFAAIDLG 98 Query: 86 GTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIVFHGPLLHLLFGAASPEVMS 145 TVVVA G + L A + + F +++++ G A+PEV Sbjct: 99 TTVVVAFSLGKRDRRRARAAARQSLVIMTLFAVVLAVVIHYFGSEIINIVAGEATPEVKG 158 Query: 146 NARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLIMNFSYVLLNFVFINGL--- 202 A TYL + LSY A+ GALRG G T++ L+++ MN ++++ + I G Sbjct: 159 LALTYLELTVLSYPAAAIALIGSGALRGAGNTKIPLMINGGMNILNIIISSILIYGAFSW 218 Query: 203 -HMGVFGMTIAINASRYLAAVCSL-IYLFRLDSSLRVKIRDLLA-LNWTMIKRIMFIGLP 259 +G G + + SRY+ AV + + + + +LR+ ++ L LN+ +I +M IG+P Sbjct: 219 QGLGFAGAGLGLTISRYIGAVAIIWVLMIGFNPALRIPLKSYLKPLNFGIIWEVMGIGIP 278 Query: 260 FAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIPANALSLTLITVVGQCMG 319 + E + FNGGK+LTQ+F+ +GT IA N I+ S+A ++ +P NAL + G+ +G Sbjct: 279 ASIESVLFNGGKLLTQMFVAGMGTNVIAGNFIAFSVAALINLPGNALGSASTIITGKRLG 338 Query: 320 SRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFHPPEEIVRDIFIVFLINSIA 379 + A + ++ P F L + F+ E+ V+++ V L + A Sbjct: 339 TGQIGQAERQLRHVFWMSTIVLTAIAWGTAP-FAGLFASFYTQEQDVKEVVKVLLWLNAA 397 Query: 380 QIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGYILGIVLNMGIIGVWLAMNC 439 +P+W+ +++ PS + A D +F VSML MW RVV GY LGIVL MG++GVWL M Sbjct: 398 FMPIWAAAWVLPSGFKGARDVRFAMWVSMLGMWGCRVVAGYTLGIVLGMGVVGVWLGMFL 457 Query: 440 EWGVRGFIFLRRFLGEKWVQH 460 +W VRG +F R + +W+ Sbjct: 458 DWAVRGALFYWRLISGRWLWR 478 >yeeO [V] COG0534 Na+-driven multidrug efflux pump Length = 547 Score = 188 bits (477), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 129/458 (28%), Positives = 228/458 (49%), Gaps = 7/458 (1%) Query: 7 NVKQGRLFLDKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXN 66 V G + K S + + +R+I L +PI ++ A V+ + V++T Sbjct: 72 QVVHGTRWHAKRKSYKVLFWREITPLAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVG 131 Query: 67 MIDSLNIFLISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIV 126 + DS N+ +++ F A+ G TVVVA G + L A + Sbjct: 132 LADSFNMVIMAFFAAIDLGTTVVVAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHH 191 Query: 127 FHGPLLHLLFGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLI 186 F ++ + G A+ EV + A TYL + LSY A+ GALRG G T++ L+++ Sbjct: 192 FGEQIIDFVAGDATTEVKALALTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGS 251 Query: 187 MNFSYVLLNFVFINGLH----MGVFGMTIAINASRYLAAVCSLIYL-FRLDSSLRVKIRD 241 +N ++++ + I GL +G G + + SRY+ AV L L + +LR+ ++ Sbjct: 252 LNILNIIISGILIYGLFSWPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKS 311 Query: 242 LL-ALNWTMIKRIMFIGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQ 300 LN+++I +M IG+P + E + F G++LTQ+F+ +GT IA N I+ S+A ++ Sbjct: 312 YFKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALIN 371 Query: 301 IPANALSLTLITVVGQCMGSRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFH 360 +P +AL + G+ +G A ++ P + S + Sbjct: 372 LPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYT 431 Query: 361 PPEEIVRDIFIVFLINSIAQIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGY 420 ++ + I+ +N++ +P+WS S++ P+ + A D+++ VSMLSMW RVV+GY Sbjct: 432 QDPQVKHVVVILIWLNALF-MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGY 490 Query: 421 ILGIVLNMGIIGVWLAMNCEWGVRGFIFLRRFLGEKWV 458 +LGI+L G++GVW+ M +W VR +F R + +W+ Sbjct: 491 VLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWL 528 >YPO1571 [V] COG0534 Na+-driven multidrug efflux pump Length = 476 Score = 166 bits (421), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 117/460 (25%), Positives = 222/460 (48%), Gaps = 7/460 (1%) Query: 5 KVNVKQGRLFLDKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXX 64 K+ + + ++ K S + +R+I L P+ ++ V+ + V +T Sbjct: 11 KLKIFRESVWYKKRKSSRVLFWREITPLAFPLFIEGLCVLLMGVFSTLLVSWLGKEAMAA 70 Query: 65 XNMIDSLNIFLISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFG 124 + DS N+ + + F +V+ G +VVVA G L++ + + Sbjct: 71 VGLADSFNMLIAAFFASVALGTSVVVAFSLGQRKRQQAQTAARQSMTLLVLISLLLVVLV 130 Query: 125 IVFHGPLLHLLFGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLS 184 ++ L+ A P V + A TYL + +Y +A+ CGALRG G TR+ ++++ Sbjct: 131 HFSGQTIIDLMANQADPSVKAMALTYLHFTVWNYPAMAITLVGCGALRGAGNTRLPMLIN 190 Query: 185 LIMNFSYVLLNFVFINGLH----MGVFGMTIAINASRYLAAVCSLIYL-FRLDSSLRVKI 239 + MN ++++ + I GL +G G I I +R A+ ++ L + +++LR+ Sbjct: 191 IGMNILNIVISSILIYGLFNWQGLGFIGAGIGITLARSAGALVVVVLLIYGANNTLRIPF 250 Query: 240 RD-LLALNWTMIKRIMFIGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGV 298 + + ++ ++ IG+P + E + FN GK++TQ F+ +GT IA N I+ S+ + Sbjct: 251 KAYFMPFTSAIMFEVLSIGIPASVESVMFNIGKLITQRFVAGMGTDVIAGNFIAFSIVAI 310 Query: 299 MQIPANALSLTLITVVGQCMGSRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSL 358 + +P NAL T ++G +G + +K P L SL Sbjct: 311 INLPGNALGYTSTIIIGTRLGKGQILQPIRQIKHIFWLSTLGVCFIALLSIPSAGFLASL 370 Query: 359 FHPPEEIVRDIFIVFLINSIAQIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVL 418 + ++ + + +N++ +P+W+ S++ P+ L+ A D+ +T V++ MW R++ Sbjct: 371 YTDDPAVITVVKHLIWMNALF-MPIWAASWVLPAGLKGAKDASYTMWVALAGMWGCRIIA 429 Query: 419 GYILGIVLNMGIIGVWLAMNCEWGVRGFIFLRRFLGEKWV 458 GYILG+ L G+ GVW+ M +W VRG +F RR +W+ Sbjct: 430 GYILGVTLGFGVTGVWMGMFLDWMVRGVLFYRRMSTGRWL 469 >CAC1611 [V] COG0534 Na+-driven multidrug efflux pump Length = 1091 Score = 154 bits (388), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 16/311 (5%), Positives = 46/311 (14%), Gaps = 9/311 (2%) Query: 27 RQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXNMIDSLNIFLISVFIAVSTGG 86 ++ I + V + + + Sbjct: 6 QKDIQGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTCYRLVW 65 Query: 87 TVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIVFHGPLLHLLFGAASPEVMSN 146 + + + L A + N Sbjct: 66 NHMTGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTLANHDN 125 Query: 147 ARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLIMNFSYVLLNFVFINGLH--- 203 + + ++ A G + + S L + Sbjct: 126 QIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGSVSDTV 185 Query: 204 ------MGVFGMTIAINASRYLAAVCSLIYLFRLDSSLRVKIRDLLALNWTMIKRIMFIG 257 + G + + + + + T Sbjct: 186 ISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVVNTGAE 245 Query: 258 LPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIPANALSLTLITVVGQC 317 AE + A ++ V+ + Sbjct: 246 GGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLN 305 Query: 318 MGSRNTKDARK 328 G + + Sbjct: 306 GGYQYVHNGGT 316 >ECs2782 [V] COG0534 Na+-driven multidrug efflux pump Length = 1091 Score = 113 bits (282), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 17/327 (5%), Positives = 43/327 (13%), Gaps = 6/327 (1%) Query: 7 NVKQGRLFLDKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXN 66 + ++ + V A +V Sbjct: 71 AFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTLANHDNQIVFG 130 Query: 67 MIDSLNIFLISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIV 126 + + I + + T G + Sbjct: 131 TTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGG 190 Query: 127 FHGPLLHLLFGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLI 186 + + + ++ + + Sbjct: 191 GQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDA 250 Query: 187 MNFSYVLLNFVFINGLH----MGVFGMTIAINASRYLAAVCSLIYL-FRLDSSLRVKIRD 241 N + + A LD++L + Sbjct: 251 ENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQY 310 Query: 242 LLA-LNWTMIKRIMFIGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQ 300 + + + N V G A Sbjct: 311 VHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTST 370 Query: 301 IPANALSLTLITVVGQCMGSRNTKDAR 327 L + N Sbjct: 371 AATVTGINRLGAFSVVEGKADNVVLEN 397 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.143 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,650,018 Number of extensions: 987972 Number of successful extensions: 2728 Number of sequences better than 1.0e-05: 70 Number of HSP's gapped: 2657 Number of HSP's successfully gapped: 70 Length of query: 463 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 353 Effective length of database: 25,691,691 Effective search space: 9069166923 Effective search space used: 9069166923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 118 (50.1 bits)