BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0019.p (427 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yaaH [R] COG3858 Predicted glycosyl hydrolase 568 e-162 BH2292 [R] COG3858 Predicted glycosyl hydrolase 483 e-136 CAC2747 [R] COG3858 Predicted glycosyl hydrolase 351 2e-96 BS_ydhD [R] COG3858 Predicted glycosyl hydrolase 237 3e-62 CAC1556 [R] COG3858 Predicted glycosyl hydrolase 172 1e-42 >BS_yaaH [R] COG3858 Predicted glycosyl hydrolase Length = 188 Score = 568 bits (1463), Expect = e-162, Method: Compositional matrix adjust. Identities = 0/424 (0%), Positives = 0/424 (0%), Gaps = 1/424 (0%) Query: 5 VVQAGQSLYGIAQAYGVNADEISEANKLSAPGRLVVGQTLVIPITGMYYWVQPGDSLYTI 64 + + G I L + G + G Y Sbjct: 1 MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIFYGGIAQIFAGLLEYKK 60 Query: 65 ARRFGVGMSDLAASNQLSLNQPLQIGFRLYIPPGPKRKAEINAYIEPRG-EVSQSLQNAA 123 FG+ S L+L L + + + + Sbjct: 61 GNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVYLGLWGVFTLFMFFGTLKG 120 Query: 124 AEAAPHLTYLAPFSFRVQRDGSLEAPPLDDLVSTAAQHRVTMMMVVTNLENARFSSGLGR 183 A + + F + G++ + + + Sbjct: 121 ARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLICGASAIYLAMGEVLNEQFGRT 180 Query: 184 IILNDQAVQSRLLENIISTAKKLGFRDIHFDFEFLRPEDREAYNAFLRKAADRIHQEGFL 243 Sbjct: 181 240 Query: 244 LSTALAPKTSRTQTGAWYTAHDYKVHGEVADFVIIMTYEWGYSGGPPMPVSPIGPVRKVL 303 Sbjct: 241 300 Query: 304 EYALSEMPGSKIMMGQNLYGYDWTLPYVAGGPYAKAISPQAAIDLARTRNAAIQYDYKDQ 363 Sbjct: 301 360 Query: 364 APHFDYTDDAGKPHKVWFEDARSIQAKFTLLKELGLRGISYWKLGLPFPQNWLLIEDNFQ 423 Sbjct: 361 420 Query: 424 VVKR 427 Sbjct: 421 424 >BH2292 [R] COG3858 Predicted glycosyl hydrolase Length = 426 Score = 483 bits (1244), Expect = e-136, Method: Compositional matrix adjust. Identities = 232/426 (54%), Positives = 304/426 (71%), Gaps = 1/426 (0%) Query: 1 MQIHVVQAGQSLYGIAQAYGVNADEISEANKLSAPGRLVVGQTLVIPITGMYYWVQPGDS 60 MQIHVVQ +S++ IA YGVNA+E+ AN++ P LV GQT+VIPI G YY+VQPGDS Sbjct: 1 MQIHVVQENESVFSIANLYGVNANELIMANEIPNPDTLVPGQTMVIPIVGRYYFVQPGDS 60 Query: 61 LYTIARRFGVGMSDLAASNQLSLNQPLQIGFRLYIPPGPKRKAEINAYIEPRGEVSQSLQ 120 LY+IARRF + +A NQL++N+PLQ G RLY+PP PK + E+N YIEPR + ++ Sbjct: 61 LYSIARRFNTTVEQIAQINQLNVNEPLQPGLRLYLPPQPKTEIEVNGYIEPRPPL-ETEP 119 Query: 121 NAAAEAAPHLTYLAPFSFRVQRDGSLEAPPLDDLVSTAAQHRVTMMMVVTNLENARFSSG 180 + P LTY++ FS++V R+G L P + + +A + ++V+TN+E F+ Sbjct: 120 RVISRIGPWLTYVSVFSYQVTREGELIPVPDEPSLEASALFAIAPVLVITNIEGDNFNPE 179 Query: 181 LGRIILNDQAVQSRLLENIISTAKKLGFRDIHFDFEFLRPEDREAYNAFLRKAADRIHQE 240 LG IL +A+Q ++L IS ++ G+R ++ DFE LRPEDRE YNAFLRKAADR+ Sbjct: 180 LGEAILQSEALQDKVLTEAISVMEEKGYRSLNIDFEHLRPEDREPYNAFLRKAADRLRAT 239 Query: 241 GFLLSTALAPKTSRTQTGAWYTAHDYKVHGEVADFVIIMTYEWGYSGGPPMPVSPIGPVR 300 G+ LSTALAPK Q GAWY AHDY HGE+ DF ++MTYEWG+SGGPPM V+P+ VR Sbjct: 240 GYTLSTALAPKVGPEQVGAWYEAHDYPAHGEIVDFTVLMTYEWGWSGGPPMAVAPLDQVR 299 Query: 301 KVLEYALSEMPGSKIMMGQNLYGYDWTLPYVAGGPYAKAISPQAAIDLARTRNAAIQYDY 360 +V+EYA+S MP +K+MMG LYGYDWTLPY GG +A+AISPQ A+ +A A IQYD Sbjct: 300 RVVEYAVSVMPANKVMMGIPLYGYDWTLPYEPGGEFARAISPQEALLIAARYGATIQYDE 359 Query: 361 KDQAPHFDYTDDAGKPHKVWFEDARSIQAKFTLLKELGLRGISYWKLGLPFPQNWLLIED 420 Q+P F Y D G+ H+VWFEDARSI+AKF L+KELGLRG+SYW L + PQNWLLI++ Sbjct: 360 TAQSPFFTYRDVEGRDHEVWFEDARSIEAKFQLVKELGLRGVSYWVLRIEIPQNWLLIQE 419 Query: 421 NFQVVK 426 +F V K Sbjct: 420 HFSVRK 425 >CAC2747 [R] COG3858 Predicted glycosyl hydrolase Length = 429 Score = 351 bits (900), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 188/433 (43%), Positives = 267/433 (61%), Gaps = 13/433 (3%) Query: 1 MQIHVVQAGQSLYGIAQAYGVNADEISEANKLSAPGRLVVGQTLVIPITGMYYWVQPGDS 60 M+I+VV+ G S+Y IA+ + V I +AN L RLVVGQ +V+P T + Y V+ GD+ Sbjct: 1 MRIYVVKPGDSVYTIARRFRVTPQSILDANNLQN-NRLVVGQAIVVPSTEITYRVRQGDT 59 Query: 61 LYTIARRFGVGMSDLAASNQLSLNQPLQIGFRLYIPPGPKR--KAEINAYIEPRGEVSQS 118 L++IARRF V + N + +Q G + IP K E+NA+I+P + Sbjct: 60 LWSIARRFRVTPKSITDLNNIENPSQIQPGLVIRIPENAKSYGDIEVNAFIQP--STPEK 117 Query: 119 LQNAAAEAAPHLTYLAPFSFRVQRDGSLEAPPLDD--LVSTAAQHRVTMMMVVTNLEN-- 174 + A P+LTY+ PFS V L PLDD +++ A + V M+ VTN+ Sbjct: 118 ENTVLSGALPYLTYVTPFSHHVTETAGLT--PLDDENIINQAQRAGVKPMLSVTNITGTA 175 Query: 175 -ARFSSGLGRIILNDQAVQSRLLENIISTAKKLGFRDIHFDFEFLRPEDREAYNAFLRKA 233 + F+S L ILN +++ + L+ NI++ + G+ + DFE + P+DRE YN FLRK Sbjct: 176 GSNFNSELISNILNSESLSNTLITNILNLIRAKGYYGVVVDFERIPPKDREKYNDFLRKL 235 Query: 234 ADRIHQEGFLLSTALAPKTSRTQTGAWYTAHDYKVHGEVADFVIIMTYEWGYSGGPPMPV 293 +H + ++TALAPKT G+W+ AHDY+ HG++ DFVIIMTYEWG+SGGPP+ V Sbjct: 236 VAALH-PNYTVATALAPKTYDITEGSWHGAHDYRAHGQIVDFVIIMTYEWGWSGGPPLAV 294 Query: 294 SPIGPVRKVLEYALSEMPGSKIMMGQNLYGYDWTLPYVAGGPYAKAISPQAAIDLARTRN 353 +PI V KV+ YA+S +P SKIMMG LYGYDWTLPYV G +A++I A+ A Sbjct: 295 APIDEVEKVIRYAVSVIPPSKIMMGIPLYGYDWTLPYVPKGEFAESIGNDEAVQRAGRYG 354 Query: 354 AAIQYDYKDQAPHFDYTDDAGKPHKVWFEDARSIQAKFTLLKELGLRGISYWKLGLPFPQ 413 A I+YD K QAP+++Y D G+ H VWFEDARS++ K+ L+ GLRG+SYW L PF Q Sbjct: 355 AIIKYDTKSQAPYYNYVDANGRQHVVWFEDARSVEEKYKLVSRYGLRGVSYWVLAQPFVQ 414 Query: 414 NWLLIEDNFQVVK 426 N+ ++ + F + K Sbjct: 415 NFRVLNNMFNITK 427 >BS_ydhD [R] COG3858 Predicted glycosyl hydrolase Length = 115 Score = 237 bits (605), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 0/412 (0%), Positives = 0/412 (0%), Gaps = 13/412 (3%) Query: 1 MQIHVVQAGQSLYGIAQAYGVNADEISEANKLSAPGRLVVGQTLVIPITGMYYWVQPGDS 60 M + + + + + + SA + Y + Sbjct: 1 MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQA-VQALAACGERF--AYVDILQNPD 57 Query: 61 LYTIARRFGVGMSDLAASNQLSLNQPLQIGFRLYIPPGPKRKA-EINAYIEPRGEVSQSL 119 + ++ + L I +Y ++ Sbjct: 58 IRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIK 117 Query: 120 QNAAAEAAPHLTYLAPFSFRVQRDGSLEAPPLDDL--VSTAAQHRVTMMMVVTNLENARF 177 Sbjct: 118 ---- 173 Query: 178 SSGLGRIILNDQAVQSRLLENIISTAKKLGFRDIHFDFEFLRPEDREAYNAFLRKAADRI 237 Sbjct: 174 233 Query: 238 HQEGFLLSTALAPKTSRTQTGAWYTAHDYKVHGEVADFVIIMTYEWGYSGGPPMPVSPIG 297 Sbjct: 234 -- 291 Query: 298 PVRKVLEYALSEMPGSKIMMGQNLYGYDWTLPYVAGGPYAKAISPQAAIDLARTRNAAIQ 357 Sbjct: 292 - 350 Query: 358 YDYKDQAPHFDYTDDAGKPHKVWFEDARSIQAKFTLLKELGLRGISYWKLGL 409 Sbjct: 351 402 >CAC1556 [R] COG3858 Predicted glycosyl hydrolase Length = 446 Score = 172 bits (436), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 91/303 (30%), Positives = 152/303 (50%), Gaps = 10/303 (3%) Query: 121 NAAAEAAPHLTYLAPFSFRVQRDGSLEAPPLDDLVSTAAQHRVTMMMVVTNLENARFSSG 180 N+ A + +A ++ G++ + V+ A + + + + N F Sbjct: 153 NSIAANTSTIDEIATHTYTTDGVGNISGLVPTNQVTYANNNGIKTLATIEN----NFDGA 208 Query: 181 LGRIILNDQAVQSRLLENIISTAKKLGFRDIHFDFEFLRPEDREAYNAFLRKAADRIHQE 240 + + +L + + L+ NI+++ K G++ ++ D E + DR F+ + ++ + Sbjct: 209 VAQTLLENSTNRQNLINNILTSLKANGYKGVNIDLEGVYYYDRSYLTTFMNELYSTLNPQ 268 Query: 241 GFLLSTALAPKTSRTQTGAWYTAHDYKVHGEVADFVIIMTYEWGYSGGPPMPVSPIGPVR 300 GF ++ + KTS + T AW A DY + AD VI+MTY+ Y GG P P++ IG V Sbjct: 269 GFYVTICVPAKTSDSPTAAWNGAFDYAALAKAADQVILMTYDEHYGGGSPGPIASIGWVE 328 Query: 301 KVLEYALSEMPGSKIMMGQNLYGYDWTLPYVAGGPYAKAISPQAAIDLARTRNAAIQYDY 360 V++YA++ +P +KIM+G YGYDW + P KA +LA T NA I +D Sbjct: 329 NVIKYAITVIPTTKIMLGAAAYGYDW-----SSNP-TKAYGISGTYNLASTYNATINWDL 382 Query: 361 KDQAPHFDYTDDAGKPHKVWFEDARSIQAKFTLLKELGLRGISYWKLGLPFPQNWLLIED 420 Q+P+F Y D +G H VWFE+ +S+ K L+ L G++ W+LGL W I Sbjct: 383 TSQSPYFYYVDSSGINHSVWFENGQSLNYKLDLINSYNLSGVAIWRLGLENSDYWTSIRT 442 Query: 421 NFQ 423 F Sbjct: 443 KFN 445 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,342,062 Number of extensions: 1279748 Number of successful extensions: 3562 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 3572 Number of HSP's successfully gapped: 14 Length of query: 427 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 318 Effective length of database: 25,807,025 Effective search space: 8206633950 Effective search space used: 8206633950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)