BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0017.p (929 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0025 [NU] COG3539 P pilus assembly protein, pilin FimA 222 2e-57 TM0025 [G] COG1472 Beta-glucosidase-related glycosidases 222 2e-57 YPO2803 [G] COG1472 Beta-glucosidase-related glycosidases 186 2e-46 CC0968 [G] COG1472 Beta-glucosidase-related glycosidases 136 1e-31 CC1756 [G] COG1472 Beta-glucosidase-related glycosidases 132 4e-30 >STM0025 [NU] COG3539 P pilus assembly protein, pilin FimA Length = 181 Score = 222 bits (566), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 0/317 (0%), Positives = 0/317 (0%), Gaps = 22/317 (6%) Query: 497 FIAQLSDQDLAVIVRGEGMSSPLVTPGTASA-FGGVSDQLFNYGIPVACTADGPSGIRM- 554 + + I + + D Sbjct: 7 ALFAMGLMLCPQINASADDLAYNLEFTGTIVAQTCDIDISSLSQSIDLGQFAVGDFPSTG 66 Query: 555 ------DSGHKATQVPIGTLLAATWNAELVEELYVMEGRELVRNGVDTLLGPGLNIRRSP 608 G A W + + Sbjct: 67 TTTKFKPFNINLKNCSRGITGAKIWFTGEPDPDNPALLAITDTGMGSGNMLASGVGVEIL 126 Query: 609 LNGRNFEYFSEDPLISGVFAAACTRGIMKGGSNATLKHFACNNQEKHRSKVDAIVSERAV 668 Sbjct: 127 186 Query: 669 REIYLKGFEIAVKEGGANSVMTSYNPVNGHWAASNYDLNTTILRGEWGFQGIVMTDWWAI 728 Sbjct: 187 246 Query: 729 MNDVVDGGPADRKNTNWMVRAQNDLYMVVSNYGSEVNAYD--DNTLDSLDNGTLTRGELQ 786 Sbjct: 247 ------------ 294 Query: 787 RSAMNICRFIMNAPVFS 803 Sbjct: 295 311 Score = 198 bits (503), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 0/408 (0%), Positives = 0/408 (0%), Gaps = 38/408 (9%) Query: 10 LEGFAEFSRTVGAEGAVLLSNEGQVLPLRNGETVSVFGRTQVNXXXXXXXXXXXXXXXXX 69 Sbjct: 321 - 379 Query: 70 XNLLDGLRSKKNLAVNEELAAVYEKWI-------EHNPFDNGGGAWAAEPWHQKEMPLTD 122 Sbjct: 380 ---- 435 Query: 123 ELVAQARSKSDKAIIIIGRTAGEDQDNADAPGSYQLTADEQTMLKQVTAYF----EQTIV 178 Sbjct: 436 495 Query: 179 VLNVSNIMDMSWLNDTSYAHPIPCVLYSWHGGMEGGNAIADVLSGEVTPSGKLTDTIAHS 238 Sbjct: 496 ----- 550 Query: 239 INDYPSTANYG----NEFKNFYQEDIYVGYRYFETFCPEKVHFEFGYGISYTTFNIEPEE 294 Sbjct: 551 - 609 Query: 295 ARLVSRDGKKYAEIAVNVTNTGTVYAGKEVVQVYYEAPQGKLGQPAKVLAAFGKTGVLQP 354 Sbjct: 610 ----- 664 Query: 355 GESQRLLVSFPVHSMASYDDAGVTGHPSAYVLEAGTYRIYAGSSIKKL 402 Sbjct: 665 ------- 705 >TM0025 [G] COG1472 Beta-glucosidase-related glycosidases Length = 721 Score = 222 bits (566), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 124/317 (39%), Positives = 179/317 (56%), Gaps = 22/317 (6%) Query: 497 FIAQLSDQDLAVIVRGEGMSSPLVTPGTASA-FGGVSDQLFNYGIPVACTADGPSGIRM- 554 ++QL+ ++ +V G G+ P + A G + + GIP ADGP+G+R+ Sbjct: 7 ILSQLTTEEKVKLVVGVGLPGLFGNPHSRVAGAAGETHPVPRLGIPAFVLADGPAGLRIN 66 Query: 555 ------DSGHKATQVPIGTLLAATWNAELVEELYVMEGRELVRNGVDTLLGPGLNIRRSP 608 ++ + T P+ +LA+TWN +L+EE+ G E+ GVD LL P +NI R+P Sbjct: 67 PTRENDENTYYTTAFPVEIMLASTWNRDLLEEVGKAMGEEVREYGVDVLLAPAMNIHRNP 126 Query: 609 LNGRNFEYFSEDPLISGVFAAACTRGIMKGGSNATLKHFACNNQEKHRSKVDAIVSERAV 668 L GRNFEY+SEDP++SG A+A +G+ G A +KHF NNQE +R VD IVSERA+ Sbjct: 127 LCGRNFEYYSEDPVLSGEMASAFVKGVQSQGVGACIKHFVANNQETNRMVVDTIVSERAL 186 Query: 669 REIYLKGFEIAVKEGGANSVMTSYNPVNGHWAASNYDLNTTILRGEWGFQGIVMTDWWAI 728 REIYLKGFEIAVK+ +VM++YN +NG + + N L +LR EWGF G VM+DW+A Sbjct: 187 REIYLKGFEIAVKKARPWTVMSAYNKLNGKYCSQNEWLLKKVLREEWGFDGFVMSDWYAG 246 Query: 729 MNDVVDGGPADRKNTNWMVRAQNDLYMVVSNYGSEVNAYD--DNTLDSLDNGTLTRGELQ 786 N V ++A ND+ M Y D + +++L G L+ L Sbjct: 247 DNPVE------------QLKAGNDMIMPGKAYQVNTERRDEIEEIMEALKEGKLSEEVLD 294 Query: 787 RSAMNICRFIMNAPVFS 803 NI + ++NAP F Sbjct: 295 ECVRNILKVLVNAPSFK 311 Score = 198 bits (503), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 139/408 (34%), Positives = 222/408 (54%), Gaps = 38/408 (9%) Query: 10 LEGFAEFSRTVGAEGAVLLSNEGQVLPLRNGETVSVFGRTQVNXXXXXXXXXXXXXXXXX 69 LE AE + GAEG VLL N G VLP V+VFG Q+ Sbjct: 321 LESHAEVAYEAGAEGVVLLENNG-VLPFDENTHVAVFGTGQIETIKGGTGSGDTHPRYTI 379 Query: 70 XNLLDGLRSKKNLAVNEELAAVYEKWI-------EHNPFDNGGGAWAAEPWHQKEMPLTD 122 ++L+G++ ++N+ +EELA+ YE++I E+ P + G +P E L++ Sbjct: 380 -SILEGIK-ERNMKFDEELASTYEEYIKKMRETEEYKPRTDSWGT-VIKP-KLPENFLSE 435 Query: 123 ELVAQARSKSDKAIIIIGRTAGEDQDNADAPGSYQLTADEQTMLKQVTAYF----EQTIV 178 + + +A K+D A+++I R +GE D G + L+ DE ++K V+ F ++ +V Sbjct: 436 KEIKKAAKKNDVAVVVISRISGEGYDRKPVKGDFYLSDDELELIKTVSKEFHDQGKKVVV 495 Query: 179 VLNVSNIMDMSWLNDTSYAHPIPCVLYSWHGGMEGGNAIADVLSGEVTPSGKLTDTIAHS 238 +LN+ + ++++ S+ + +L W G E G +ADVL G++ PSGKL T Sbjct: 496 LLNIGSPIEVA-----SWRDLVDGILLVWQAGQEMGRIVADVLVGKINPSGKLPTTFPKD 550 Query: 239 INDYPSTANYG----NEFKNFYQEDIYVGYRYFETFCPEKVHFEFGYGISYTTFNIEPEE 294 +D PS G N + Y+EDIYVGYRY++TF E +EFGYG+SYT F + + Sbjct: 551 YSDVPSWTFPGEPKDNPQRVVYEEDIYVGYRYYDTFGVEPA-YEFGYGLSYTKFEYKDLK 609 Query: 295 ARLVSRDGKKYAEIAVNVTNTGTVYAGKEVVQVYYEAPQGKLGQPAKVLAAFGKTGVLQP 354 ++ DG+ ++ +TNTG AGKEV QVY +AP+GK+ +P + L AF KT +L P Sbjct: 610 ---IAIDGETL-RVSYTITNTGD-RAGKEVSQVYIKAPKGKIDKPFQELKAFHKTKLLNP 664 Query: 355 GESQRLLVSFPVHSMASYDDAGVTGHPSAYVLEAGTYRIYAGSSIKKL 402 GES+ + + P+ +AS+D +V+E+G Y + G+S + + Sbjct: 665 GESEEISLEIPLRDLASFDG-------KEWVVESGEYEVRVGASSRDI 705 >YPO2803 [G] COG1472 Beta-glucosidase-related glycosidases Length = 793 Score = 186 bits (471), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 112/319 (35%), Positives = 172/319 (53%), Gaps = 24/319 (7%) Query: 494 MTTFIAQLSDQDLAVIVRGEGMSSPLVTPGTASAFGGVSDQLFNYGIPVACTADGPSGIR 553 + + IA ++ ++ A V G GM++ G A G + + GIP ADGP G+R Sbjct: 31 IPSVIAAMTLEEKAAFVSGTGMNNAEKVAGAA----GSTLAIPRLGIPQIVFADGPVGVR 86 Query: 554 MDSGHK------ATQVPIGTLLAATWNAELVEELYVMEGRELVRNGVDTLLGPGLNIRRS 607 + +G AT P+ +AATW+ +++ + G E + GVD +LGP +NI+R Sbjct: 87 LGAGPTGGEKRFATGFPVSVAMAATWDPAIIKRVGGAIGDEAKQYGVDLILGPAINIQRH 146 Query: 608 PLNGRNFEYFSEDPLISGVFAAACTRGIMKGGSNATLKHFACNNQEKHRSKVDAIVSERA 667 PLNGRNFEYF+EDPL++ + A+ G+ G A LKHFA NNQE R V+ I+S+RA Sbjct: 147 PLNGRNFEYFTEDPLLNAIMTASYIDGMQTEGVGAVLKHFAANNQETRRQIVNEIISDRA 206 Query: 668 VREIYLKGFEIAVKEGGANSVMTSYNPVNGHWAASNYDLNTTILRGEWGFQGIVMTDWWA 727 + EIY GFE A+++ +VM+SY +NG ++ N L +LR +W F G VM+DW+ Sbjct: 207 LHEIYFPGFEYAMEKAQPWAVMSSYPSINGTPSSQNPWLLKDVLRQQWKFDGFVMSDWYG 266 Query: 728 IMNDV--VDGGPADRKNTNWMV--RAQNDLYMVVSNYGSEVNAYDDNTLDSLDNGTLTRG 783 + + V + GG N M R +D + N + D L +L +G LT+ Sbjct: 267 VADPVSALKGG-----NDLNMPGGRTPDDSLFLTPNTDPK-----DVVLAALKSGELTQD 316 Query: 784 ELQRSAMNICRFIMNAPVF 802 ++ + NI ++ F Sbjct: 317 QIDENIRNILNVVIKTHRF 335 Score = 144 bits (364), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 119/398 (29%), Positives = 186/398 (46%), Gaps = 55/398 (13%) Query: 16 FSRTVGAEGAVLLSNEGQVLPLRNGETVSVFGRTQVNXXXXXXXXXXXXXXXXXXNLLDG 75 +R + A+ VLL N+ LP++ V+ FG+ N Sbjct: 352 LARQIAADSMVLLKNDAHTLPIKAAFRVAAFGQNIDNFFVTGGGSAEV-----------N 400 Query: 76 LRSKKNLAVNEELAAVYEKWIEHNPFDNGGGAWAAEPWHQK-EMPLTDELVAQARSKSDK 134 + K+ + + + L N G A + Q + P+ + +A +SD Sbjct: 401 IDPKRLVTLPQGL-------------KNAGIALIRQIGDQALDEPVNQPALTKAAQQSDI 447 Query: 135 AIIIIGRTAGEDQDNADAPGSYQLTADEQTMLKQVTAYFEQ----TIVVLNVSNIMDMSW 190 A+I IGR++ E D S + DE M+K+V + F Q +V+LN+ + ++M Sbjct: 448 ALISIGRSSTEGADRY----SMAMHPDEVAMIKKVASAFHQQNKKVVVLLNIGSPIEM-- 501 Query: 191 LNDTSYAHPIPCVLYSWHGGMEGGNAIADVLSGEVTPSGKLTDTIAHSINDYPSTANY-G 249 TS+ +L +W G + GNA+AD+L G+V PSGKL T + D PS NY G Sbjct: 502 ---TSWEQYADAILLTWQPGEQAGNAVADILLGKVNPSGKLPLTFPKRLEDSPSFGNYPG 558 Query: 250 NEFKNFYQEDIYVGYRYFET--FCPEKVHFEFGYGISYTTFN---IEPEEARLVSRDGKK 304 N Y E IYVGYRYF+T P + FGYG+SY+T N I PE+ + + D + Sbjct: 559 NAKTVIYGEGIYVGYRYFDTKRIAP---MYPFGYGLSYSTVNYGKISPEKP-VFNIDTEN 614 Query: 305 YAEIAVNVTNTGTVYAGKEVVQVYYEAPQGKLGQPAKVLAAFGKTGVLQPGESQRLLVSF 364 E+++ V NT + KEVVQ+Y +L +P + L AF K L GE +R+ Sbjct: 615 SIEVSIPVRNTSGIDT-KEVVQLYVHDNASRLDRPEQELKAFEKVS-LSAGEEKRVAFKL 672 Query: 365 PVHSMASYDDAGVTGHPSAYVLEAGTYRIYAGSSIKKL 402 + + YD+ + +VLE G + + G S + + Sbjct: 673 DKRAFSYYDE-----DKNNWVLEPGLFTLRIGRSSRDI 705 >CC0968 [G] COG1472 Beta-glucosidase-related glycosidases Length = 758 Score = 136 bits (343), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 81/214 (37%), Positives = 112/214 (52%), Gaps = 8/214 (3%) Query: 518 PLVTPGTASAFGGVSDQLFNYGIPVACTADGPSGIRMDSGHKA----TQVPIGTLLAATW 573 P PG+A GV GIP D G+ K T +P G ATW Sbjct: 85 PESLPGSAGYIAGVP----RLGIPAQFQTDAGVGVATQGSAKEFRERTALPSGMATTATW 140 Query: 574 NAELVEELYVMEGRELVRNGVDTLLGPGLNIRRSPLNGRNFEYFSEDPLISGVFAAACTR 633 N EL + M G+E +G + L G+N+ R P NGRNFEY EDPL++GV AA R Sbjct: 141 NPELAFQGGAMIGKEARDSGFNVQLAGGVNLVREPRNGRNFEYGGEDPLLAGVMVAAQIR 200 Query: 634 GIMKGGSNATLKHFACNNQEKHRSKVDAIVSERAVREIYLKGFEIAVKEGGANSVMTSYN 693 GI +T+KH+A N QE R V + + + A R L F+ A+++ +SVM +YN Sbjct: 201 GIQSNNIISTIKHYALNAQETGRFVVSSNIDDTAARMSDLLAFQFAIEQSDPHSVMCAYN 260 Query: 694 PVNGHWAASNYDLNTTILRGEWGFQGIVMTDWWA 727 VN +A + L T+L+ +WG++G VM+DW A Sbjct: 261 RVNSVYACESDWLLNTVLKKDWGYKGYVMSDWGA 294 Score = 87.0 bits (214), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 131/296 (44%), Gaps = 33/296 (11%) Query: 126 AQARSKSDKAIIIIGRTAGEDQDNADAPGSYQLTADEQTMLKQVTAYFEQTIVVLNVSNI 185 A+ + SD A++ + E D + L ++ + V + ++T+VVL Sbjct: 478 AKLAASSDLALVFADQWTTESVDVT----TLNLPKNQDATIDAVASANKKTVVVLLTGGP 533 Query: 186 MDMSWLNDTSYAHPIPCVLYSWHGGMEGGNAIADVLSGEVTPSGKLTDTIAHSINDYPST 245 + M WL+ + V+ +W G GG AIA VL+GEV SG+L T S+ + P Sbjct: 534 LLMPWLDK------VGAVVEAWFPGTAGGEAIARVLTGEVDASGRLPVTFPKSVAELPRP 587 Query: 246 ------ANYGNEFK-NFYQEDIYVGYRYFETFCPEKVHFEFGYGISYTTFNIEPEEARLV 298 G F ++ E VGY++++ E + F FG+G+SYT F + L Sbjct: 588 KLDGLGKPDGEMFDVDYTLEGAAVGYKWYDLKKIEPL-FAFGHGLSYTQFAY----SNLT 642 Query: 299 SRDGKKYAEIAVNVTNTGTVYAGKEVVQVYYEAPQGKLGQPAKVLAAFGKTGVLQPGESQ 358 + ++ V N G GK+V QVY G P K LA F K L PG SQ Sbjct: 643 ASASGDTLTVSFEVKNVGR-RPGKDVPQVYVGPKAGGWEAP-KRLAGFQKVS-LAPGASQ 699 Query: 359 RLLVSFPVHSMASYDDAGVTGHPSAYVLEAGTYRIYAGSS---IKKLAEVQVEGRS 411 R+ V+ +A +D + + AG Y I G+S I A+V + RS Sbjct: 700 RVTVTVDPRLLAVWDSKA-----HGWSIAAGQYDIALGASSRAISSTAQVTLAARS 750 >CC1756 [G] COG1472 Beta-glucosidase-related glycosidases Length = 762 Score = 132 bits (331), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 70/181 (38%), Positives = 100/181 (55%) Query: 552 IRMDSGHKATQVPIGTLLAATWNAELVEELYVMEGRELVRNGVDTLLGPGLNIRRSPLNG 611 + G AT +P G LA+T+ +L + M G E + LL G+N+ R P G Sbjct: 119 VEQRKGDVATALPSGLALASTFEPKLAFDGGAMIGAEARAKTFNVLLAGGVNLTRDPWAG 178 Query: 612 RNFEYFSEDPLISGVFAAACTRGIMKGGSNATLKHFACNNQEKHRSKVDAIVSERAVREI 671 RNFEY EDPL++G A +G+ +T+KHFA N QE R +DA + E +RE Sbjct: 179 RNFEYLGEDPLLAGEMVAEQIKGVQSNKIVSTIKHFALNAQETGRHVMDAQIDEADLRES 238 Query: 672 YLKGFEIAVKEGGANSVMTSYNPVNGHWAASNYDLNTTILRGEWGFQGIVMTDWWAIMND 731 L F+IA+++ SVM +YN VNG WA N L +L+ +W + G VM+DW A+ + Sbjct: 239 DLLAFQIAIEKSNPASVMCAYNKVNGDWACENDFLLNKVLKRDWNYPGWVMSDWGAVHST 298 Query: 732 V 732 V Sbjct: 299 V 299 Score = 101 bits (251), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 88/269 (32%), Positives = 127/269 (47%), Gaps = 49/269 (18%) Query: 151 DAPGSYQLTADEQTMLKQVTAYFEQTIVVLNVSNIMDMSWLNDTSYAHPIPCVLYSWHGG 210 DAP S L ++ +++ V+A + +VVL + M WL+ + VL +W+ G Sbjct: 499 DAP-SIALPENQDALIEAVSAANKNAVVVLETGGPVLMPWLDK------VGAVLQAWYPG 551 Query: 211 MEGGNAIADVLSGEVTPSGKLTDTIAHS-----------------INDYPSTANYGNEFK 253 GG AIA +L GEV PSG+L T S I+D K Sbjct: 552 QRGGQAIARLLFGEVNPSGRLAMTFPKSEDQAPRASAPGFAEQAAIDDARRAGQKAGPIK 611 Query: 254 NF---YQEDIYVGYRYFETFCPEKVHFEFGYGISYTTF---NIEPEEARLVSRDGKKYAE 307 F Y E VGYR+F + + FGYG+SYT+F N++ E+ DG K Sbjct: 612 GFPVRYVEGAAVGYRWFAQ-EKRRPLYPFGYGLSYTSFGYKNLKVEDG-----DGLK--- 662 Query: 308 IAVNVTNTGTVYAGKEVVQVYYEAPQGKLGQPAKVLAAFGKTGVLQPGESQRLLVSFPVH 367 ++ +VTNTG V AG + Q+Y + Q K LA F K L PGE++R+ ++ Sbjct: 663 VSFDVTNTGKV-AGADTPQLYVTSGQRKA---MLRLAGFQKVD-LAPGETKRVTLNVEPR 717 Query: 368 SMASYDDAGVTGHPSAYVLEAGTYRIYAG 396 +A YD T P + + AGTY +Y G Sbjct: 718 ILADYD----TAKP-GWTIAAGTYPLYVG 741 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 68,899,146 Number of extensions: 2869166 Number of successful extensions: 14692 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 14602 Number of HSP's successfully gapped: 47 Length of query: 929 Length of database: 38,378,431 Length adjustment: 116 Effective length of query: 813 Effective length of database: 24,999,687 Effective search space: 20324745531 Effective search space used: 20324745531 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 121 (51.2 bits)