BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0010.p (450 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmi... 71 3e-12 lin0220 [G] COG1653 ABC-type sugar transport system, periplasmic... 70 8e-12 BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 65 2e-10 all1916 [G] COG1653 ABC-type sugar transport system, periplasmic... 64 4e-10 BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasm... 58 4e-08 >SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 451 Score = 71.2 bits (173), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 76/299 (25%), Positives = 135/299 (45%), Gaps = 40/299 (13%) Query: 71 QEVIDNWNKAHPETQVEYVRFVNDDD--GNLKLDTAMVTGQDVDLFVNYTVSHAAKRVES 128 ++ + +WN +HP+ ++E + F+ + + KL TA +G+ D+F+ + + Sbjct: 68 EKTVADWNDSHPQ-KIE-LEFIPNSEYISGPKLATAFASGEGPDIFI-ISPGDFLRYYNG 124 Query: 129 NLALDLSSFTDYNIDEKMGSD-----AEGWKINGKYYGVPTTKSAFFIALNKDALDAAGL 183 + DL+ F ID+K +D ++GK +G+P + + A + AGL Sbjct: 125 GVLKDLTPF----IDDKAKADFPESVIANRMVDGKIFGIPMEVEPMAMYYSVKAFEEAGL 180 Query: 184 ---PVPKDWTWDELREYAKKLKVGDKYGFIQNMEP-FVDPID--SVLSQEG--YTKADGT 235 VPK TW+ L E AKKL +++G + +P + L Q G + ADG Sbjct: 181 NENDVPK--TWEALLEVAKKLTTPERFGVMFETQPGYYQNFTWYPFLWQGGGEFQTADGK 238 Query: 236 SNLDHP----LVRKWLETLNGMMQEDKTTPPLGEQLTSKMPVEQVFLSGEVPMLNIGAWL 291 S+ D P ++ W + +N + + G + + SG V M N+G W Sbjct: 239 SSFDSPATVQALKFWQDAINSGVAPRQIMGAGGHDTIANL------ASGYVAMQNVGIWG 292 Query: 292 LRSSNNFTDFPRDFKIAFAQVPRLADSADQYVTRGGLGDYISINANSKKQEAAWEFLKW 350 + + +DF+ ++P + +YVT GG +++ NAN +AA EF W Sbjct: 293 I---SQLQSNAKDFQYGVFRLPTPPNG--KYVTVGGGWAFVA-NANGHNSDAAGEFCAW 345 >lin0220 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 418 Score = 69.7 bits (169), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 90/370 (24%), Positives = 166/370 (44%), Gaps = 30/370 (8%) Query: 72 EVIDNWNKAHPETQVEYVRFVNDDDGNLKLDTAMVTGQDVDLF-VNYT--VSHAAKRVES 128 +++ + K +P+ +V V+ + +D KL T + G D F +NY + +A K V + Sbjct: 49 KMVKEFEKQNPDIKVN-VQTIAFNDYFTKLQTQIAGGDAPDAFELNYETFMQYAEKGVLA 107 Query: 129 NLALDLSSFTDYNIDEKMGSDAEGWKINGKYYGVPTTKSAFFIALNKDALDAAGLPVP-K 187 +L + D++ + +K +GK YG+ + S NKD D AG+ P Sbjct: 108 DLTSYIEKDKDFDPSTLNKQAYDAFKYDGKQYGMVESFSNVVTIYNKDLFDKAGVEYPTA 167 Query: 188 DWTWDELREYAKKLKVGDKYGFIQNMEPFVDPIDSVLSQEG---YTKADGTSNLDHPLVR 244 DWTW + AKKL + + ++ V +Q G + K + ++ P Sbjct: 168 DWTWKDEEAAAKKLTDAKNKVWGTSQPVTMNEFFKVAAQNGGSIFDKDMTKTTINSPENV 227 Query: 245 KWLETLNGMMQEDKTTPPLGEQLTSKMPVEQVFLSGEVPMLNIGAWLLRSSNNFTDFPRD 304 + L L + E K P + L+ ++P E +F++G++ ML+ G WL + F D P Sbjct: 228 EALTHLTNEVTESKVAPSPSD-LSGQLP-EDLFMNGQIAMLHTGIWLF---DMFQDAPFK 282 Query: 305 FKIAFAQVPRLADSADQYVTRGGLGDYISINANSKKQEAAWEFLKWYS---DGGMAPMAA 361 + + QV + G+G ++ +S K+EAA++F + S + +A Sbjct: 283 WDV---QVEAGNKQKATHFFANGIG----VSKDSDKKEAAFKFASFMSANKEAAKIRIAN 335 Query: 362 GGRLPASKDAN-QDDALNSLLGDKRDTYDLDSLMYVMFDDKTPTYVRSLPQEVMDLRSQE 420 LPA+++ L++ D R+ +SL Y++ P V+ ++ D + E Sbjct: 336 NWELPATENKEILQPYLDATPPDNREAV-FESLQYMVL----PPVVKDW-NKISDYTNSE 389 Query: 421 YEKYFLGSQS 430 +EK G + Sbjct: 390 FEKVLNGEST 399 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 65.5 bits (158), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 52/163 (31%), Positives = 79/163 (48%), Gaps = 9/163 (5%) Query: 46 TNASGSSKGTVTLKM---WGGVPPESGPQEVIDNWNKAHPETQVEYVRFVNDDDGNLKLD 102 ++ S S+ G VTLK W P +S +EV+ + K HP+ ++ +ND + K+ Sbjct: 22 SSQSSSADGKVTLKFFHRWPKEPEKSYFEEVVKEFEKDHPDIDIQIEAVLNDSYKD-KIK 80 Query: 103 TAMVTGQDVDLFVNYTVSHAAKRVESNLALDLSSFTDYNID---EKMGSDAEGWKINGKY 159 + T D++ +++ A K + N ALDLSS+ + D + + S + K Sbjct: 81 VMLGTTSPPDIYFSWSDEFAFKFIRGNKALDLSSYYKNDTDWSSQLVQSQITPFTYENKQ 140 Query: 160 YGVPTTKSAFFIALNKDALDAAGLPVPKDWTWDELREYAKKLK 202 YGVP A NKD L PK TWDEL + +KKLK Sbjct: 141 YGVPWQMDAKSFFYNKDIFQKLNLDPPK--TWDELIDVSKKLK 181 >all1916 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 418 Score = 63.9 bits (154), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 104/418 (24%), Positives = 169/418 (40%), Gaps = 66/418 (15%) Query: 55 TVTLKMWGGVPPESGP-QEVIDNWNKAHPETQVEYVRFVNDDDGNLKLDTAMVTGQDVDL 113 TV L WGG P E ++V+ ++ HP +V+Y +++ Q +D+ Sbjct: 38 TVKLSGWGGSPVEQKLLKQVLQDFEAQHPHIKVKY---------------EVISDQYMDV 82 Query: 114 FVNYTVSHAAKRVESNLALDLSSFTDYNIDEKMG---------SDAEG-----WKINGKY 159 + AA V AL+ N+ E + SD E +K Sbjct: 83 IKTRLIGEAAPDVFYLDALEAPFLMSQNVLEPLDNYITPEFDLSDFEANLLDNFKYQNHI 142 Query: 160 YGVPTTKSAFFIALNKDALDAAGLPVPKDWTWDELREYAKKLKVG-DKYGFIQ--NMEPF 216 YG P S + NK A AAGL P TW ELR Y+++L +KYGF + + Sbjct: 143 YGFPKDYSTLALFYNKKAFAAAGLSSPPA-TWTELRTYSQQLTGKLNKYGFGEAPELARQ 201 Query: 217 VDPIDS----VLSQEGYTKADGTSNLDHPLVRKWLETLNGMMQEDKTTPPLGEQLTSKMP 272 V I + V++Q GY + L + + + Q+DK++ + T+ Sbjct: 202 VYKIKAFGGEVINQNGYATFASEAGL------RGFQLVIDQYQKDKSSAQKSDVGTNSG- 254 Query: 273 VEQVFLSGEVPMLNIGAWLLRSSNNFTDFPRDFKIAFAQVPRLADSADQYVTRGGLGDYI 332 ++F +V M+ G W + FP+ + A AQ+P + + V Y+ Sbjct: 255 -SEMFGQEKVAMVIEGNWAIPYLQE--TFPQ-LEFATAQLPTINNKKGTMVFTVA---YV 307 Query: 333 SINANSKKQEAAWEFLKWYS-DGGMAP-MAAGGRLPASKDANQDDALNSLLGDKRDTYDL 390 ++ S+ + AWE + + + GM + G LP K +Q G ++D L Sbjct: 308 -MSKQSQHKAEAWELISYLTGKAGMQKWTSTGFALPTRKSVSQK------FGYEQD--PL 358 Query: 391 DSLMYVMFDDKTPTYVRSLPQEVMDLRSQEYEKYFLGSQSLNQTIDAMVARHNNFLKQ 448 S + DD TP V P +++ ++ LG Q L Q AM+ N KQ Sbjct: 359 RSPLVAGVDDATPWQVGKYPAPIVNNFDNQFVSALLGQQPLKQ---AMLRAQNQANKQ 413 >BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 398 Score = 57.8 bits (138), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 60/203 (29%), Positives = 91/203 (44%), Gaps = 21/203 (10%) Query: 155 INGKYYGVPTTKSAFFIALNKDALDAAGLPVPKDWTWDELREYAKKLKV--GDKYGF-IQ 211 ++GK YG+P S + NKD + AG+ P WDEL+ A K+K G+ YGF +Q Sbjct: 114 LDGKTYGLPIAASVRAMYYNKDLFEKAGIKNPPA-NWDELKADAAKIKALGGENYGFGLQ 172 Query: 212 NMEPFVDPIDSVLSQEGY----TKADGTSNLDHPLVRKWLETLNGMMQEDKTTPPLGEQL 267 E D + + Y DGTS L P + + M+ + T P G Sbjct: 173 GKEIETD-VYYYYAMWSYGTEILNKDGTSGLSTPGALEAAKLYKSMIDDGLTQP--GVTS 229 Query: 268 TSKMPVEQVFLSGEVPMLNIGAWLLRSSNNFTDFPRDFKIAFAQVPRLADSADQYVTRG- 326 ++ V+ +F G+V M+ +L SN + + K A +P A RG Sbjct: 230 YAREDVQNLFKQGKVGMMITAPFL---SNQIKEEAPNLKYGVAAIPAGPTGA-----RGT 281 Query: 327 -GLGDYISINANSKKQEAAWEFL 348 G+ D + + NSK +E AW+ L Sbjct: 282 YGVTDSVIMFQNSKNKEEAWKVL 304 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,849,144 Number of extensions: 1407370 Number of successful extensions: 1980 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 1989 Number of HSP's successfully gapped: 21 Length of query: 450 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 340 Effective length of database: 25,691,691 Effective search space: 8735174940 Effective search space used: 8735174940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)