BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0006.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0219 [G] COG0395 ABC-type sugar transport system, permease co... 211 1e-54 BH1119 [G] COG0395 ABC-type sugar transport system, permease com... 194 2e-49 lin0761 [G] COG0395 ABC-type sugar transport system, permease co... 179 3e-45 SMb20233 [G] COG0395 ABC-type sugar transport system, permease c... 173 3e-43 all4824 [G] COG0395 ABC-type sugar transport system, permease co... 172 4e-43 >lin0219 [G] COG0395 ABC-type sugar transport system, permease component Length = 282 Score = 211 bits (536), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 110/251 (43%), Positives = 161/251 (64%), Gaps = 3/251 (1%) Query: 25 PFLWMISTSFKTPAEVFQYPIQWIPVHFNWSHHVKVWTGENSFMLYYL-NSLKVSVLSTI 83 PF+WMIST+FKT A P Q+IP ++ +V+ E ML +L NS+ V+V++T+ Sbjct: 33 PFVWMISTAFKTGAANMVLPPQFIPKEPTTANFTQVF--EMFPMLRFLMNSVVVAVVTTL 90 Query: 84 GAVTLSSLAAYGFARIQFRGRDTMFMVYLSMMMVPPQVLFVPKFIMFDWAGIYNTHWALI 143 G + S+AAY FARI F GRD +F++YL+ MMVP QV +P+FI+ G +++ LI Sbjct: 91 GQMLFCSMAAYAFARIPFWGRDKLFLLYLATMMVPAQVTMIPQFILMKQFGWLDSYAGLI 150 Query: 144 LPGFFTIFGVFMMRQFFLSVPYEISEAAFIDGAGHFRIFSRIILPMAKPSIATLAIIDFS 203 +P F++FG F++RQ F+ +P E+ EAAF+DGA HF IF ++ILP+AKP+ ATL I+ F Sbjct: 151 VPALFSVFGTFLLRQAFMGIPKELEEAAFMDGANHFTIFRKVILPLAKPTFATLGILTFM 210 Query: 204 WHWNDYENALVFLIDHDLFTVPLGLQNFILENNVDYNGMMAAATAGIIPMIIVFLIGQKY 263 WN Y L+ ++ T+PLGL +Y MMA +IP++ V+L QKY Sbjct: 211 QSWNSYLWPLIVTSSQEMATLPLGLSLLQGRYGTNYGLMMAGVLISVIPILAVYLFAQKY 270 Query: 264 IIEGVASSAVK 274 I+G+A S +K Sbjct: 271 FIQGMAMSGMK 281 >BH1119 [G] COG0395 ABC-type sugar transport system, permease component Length = 281 Score = 194 bits (492), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 96/255 (37%), Positives = 158/255 (61%), Gaps = 5/255 (1%) Query: 25 PFLWMISTSFKTPAEVFQYPIQWIPVHFNWSHHVKVWTG--ENSFMLYYLNSLKVSVLST 82 P LWM+++S K +E+F IP F WS++ W G F +Y+ NS ++ Sbjct: 27 PILWMVASSLKPASEIFSQASSLIPSEFVWSNYPDGWAGFGRTGFDIYFKNSFIITTSVV 86 Query: 83 IGAVTLSSLAAYGFARIQFRGRDTMFMVYLSMMMVPPQVLFVPKFIMFDWAGIYNTHWAL 142 IGA+ SS+ AYGFAR+QF+ + +F ++ +M+P Q+ +P++I+F G NT + L Sbjct: 87 IGAILSSSIVAYGFARLQFKFKKVLFACLIATVMLPVQITLIPQYILFHNLGWVNTFYPL 146 Query: 143 ILPGFF--TIFGVFMMRQFFLSVPYEISEAAFIDGAGHFRIFSRIILPMAKPSIATLAII 200 I+P F T F +F++ QF +P E+ EAA IDG F +F R+ILP+ KP++ T+AI Sbjct: 147 IVPAFLGGTPFFIFLLIQFIRGIPRELDEAAIIDGCSTFGVFWRVILPLLKPALVTVAIF 206 Query: 201 DFSWHWNDYENALVFLIDHDLFTVPLGLQNFI-LENNVDYNGMMAAATAGIIPMIIVFLI 259 F W W+D+ L++L D+ TV LGL+NF+ E+ + ++A +T ++P I+FL Sbjct: 207 AFMWTWDDFLAPLIYLNKTDIQTVALGLRNFMDAESGTSWGALLAMSTLSLLPQFIIFLF 266 Query: 260 GQKYIIEGVASSAVK 274 Q+Y++EG++++ +K Sbjct: 267 FQRYLVEGISTTGLK 281 >lin0761 [G] COG0395 ABC-type sugar transport system, permease component Length = 291 Score = 179 bits (455), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 93/264 (35%), Positives = 147/264 (55%), Gaps = 15/264 (5%) Query: 25 PFLWMISTSFKTPAEVFQYPIQWIPVHFNWSHHVKVWTGENSFMLYYLNSLKVSVLSTIG 84 P +W +STSFK +E+ + IP F W ++ V++ F Y +NSL ++V + Sbjct: 29 PLIWTVSTSFKPTSEILSNSMNLIPKMFTWDNYKDVFS-TTPFARYLVNSLILAVGGVLT 87 Query: 85 AVTLSSLAAYGFARIQFRGRDTMFMVYLSMMMVPPQVLFVPKFIMFD------------- 131 + SLA Y FA++ F+G+ +FM +L MM+P V +P F++ Sbjct: 88 NLFFGSLAGYAFAKLPFKGKKGLFMTFLGSMMIPAVVTMIPSFLVLKNFPLMGGNDITGH 147 Query: 132 -WAGIYNTHWALILPGFFTIFGVFMMRQFFLSVPYEISEAAFIDGAGHFRIFSRIILPMA 190 G NT+WA+++PG F +F M+QFF ++P E+SEAA IDG F+IF +I +P+A Sbjct: 148 GGMGFINTYWAILIPGAAGAFSIFFMKQFFETLPDELSEAARIDGCSEFKIFWKIYMPLA 207 Query: 191 KPSIATLAIIDFSWHWNDYENALVFLIDHDLFTVPLGLQNFILENNVDYNGMMAAATAGI 250 K ++ATL I+ F WN + L+ L + TV +GL +F + +Y +MA Sbjct: 208 KTALATLGIMTFQAGWNQFMWPLIVLNSQKMMTVQVGLASFQYNESANYGALMAGTIVST 267 Query: 251 IPMIIVFLIGQKYIIEGVASSAVK 274 IP++ VF+ QKY +EG+A + K Sbjct: 268 IPVLFVFIFAQKYFVEGIAHNGGK 291 >SMb20233 [G] COG0395 ABC-type sugar transport system, permease component Length = 282 Score = 173 bits (439), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 97/250 (38%), Positives = 154/250 (61%), Gaps = 3/250 (1%) Query: 25 PFLWMISTSFKTPAEVFQYPIQWIPVHFNWSHHVKVWTGENSFMLYYLNSLKVSVLSTIG 84 P LW++S +FK AE F + IP + + V TG F+ Y +NS VS T+ Sbjct: 36 PLLWIVSIAFKPAAESFSSNL--IPQAPTLDNFIYVLTGV-PFIRYMVNSFLVSATVTVV 92 Query: 85 AVTLSSLAAYGFARIQFRGRDTMFMVYLSMMMVPPQVLFVPKFIMFDWAGIYNTHWALIL 144 A+ ++A Y AR++F GR+ MF+ S +V V+ VP F++ G+ N++ LI+ Sbjct: 93 ALFFHTMAGYALARLRFPGREVMFLSIFSTFLVSLPVIIVPLFVIVKAMGMLNSYAGLII 152 Query: 145 PGFFTIFGVFMMRQFFLSVPYEISEAAFIDGAGHFRIFSRIILPMAKPSIATLAIIDFSW 204 P F FG+F++RQ++LS+P EI EAA IDGAG++RI+ +ILP+++P ++ LAI+ F Sbjct: 153 PAIFNAFGIFLLRQYYLSLPKEIEEAARIDGAGYWRIYWSVILPLSRPIMSALAILFFLA 212 Query: 205 HWNDYENALVFLIDHDLFTVPLGLQNFILENNVDYNGMMAAATAGIIPMIIVFLIGQKYI 264 +WN + L D DL+ V LG+ NF + + +N MMAA+T IP +I+F+I Q+ I Sbjct: 213 NWNSFLWPLTITSDPDLWVVQLGIANFKSQYSASWNYMMAASTIVAIPTLILFVIFQRQI 272 Query: 265 IEGVASSAVK 274 ++ + +S +K Sbjct: 273 MDSLKTSGLK 282 >all4824 [G] COG0395 ABC-type sugar transport system, permease component Length = 279 Score = 172 bits (437), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 95/252 (37%), Positives = 150/252 (59%), Gaps = 2/252 (0%) Query: 25 PFLWMISTSFKTPAE-VFQYPIQWIPVHFNWSHHVKVWTGENSFMLYYLNSLKVSVLSTI 83 P LW+IST+ K+P E +FQ P Q +P + KVW F Y NS VSVL+ Sbjct: 29 PLLWLISTALKSPTENIFQSPPQLLPNQPTVDNFFKVWNSL-PFGQYLYNSTLVSVLTVG 87 Query: 84 GAVTLSSLAAYGFARIQFRGRDTMFMVYLSMMMVPPQVLFVPKFIMFDWAGIYNTHWALI 143 + SLAAY AR+ F GRD +F+ +S +M+P Q++ +P +I+ G+ NT+ ++ Sbjct: 88 LNLLFCSLAAYPLARLSFPGRDWIFIAIVSTIMIPFQIVMIPLYILTVQLGLRNTYLGMM 147 Query: 144 LPGFFTIFGVFMMRQFFLSVPYEISEAAFIDGAGHFRIFSRIILPMAKPSIATLAIIDFS 203 P + FG+F++RQ F+ VP EI EAA +DG+ ++ I+LP +P++ TLAI F Sbjct: 148 FPSLASAFGIFLLRQAFMGVPKEIEEAARMDGSSELGLWWHIMLPAVRPALVTLAIFVFI 207 Query: 204 WHWNDYENALVFLIDHDLFTVPLGLQNFILENNVDYNGMMAAATAGIIPMIIVFLIGQKY 263 W+D+ L+ + D +L+T+PLG+ ++D+ + A + I P++++FLI Q+Y Sbjct: 208 GAWSDFLWPLIVIQDENLYTLPLGVAKLAGTFSLDWRLVAAGSVIAIAPVLLLFLILQRY 267 Query: 264 IIEGVASSAVKG 275 I+E S VKG Sbjct: 268 IVETDIGSGVKG 279 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.142 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,435,926 Number of extensions: 720492 Number of successful extensions: 2324 Number of sequences better than 1.0e-05: 168 Number of HSP's gapped: 2118 Number of HSP's successfully gapped: 168 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 115 (48.9 bits)