BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0040.p (322 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA1161 [GEPR] COG0477 Permeases of the major facilitator superfa... 91 2e-18 NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe ... 91 2e-18 PAB0754 [GEPR] COG0477 Permeases of the major facilitator superf... 88 2e-17 TM0342 [GEPR] COG0477 Permeases of the major facilitator superfa... 87 5e-17 PAB0724 [GEPR] COG0477 Permeases of the major facilitator superf... 81 2e-15 >MA1161 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 443 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 49/185 (26%), Positives = 91/185 (49%) Query: 10 TPSNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFA 69 T L + G+ S++GSG+ TF +G+++ + TG+ S+ ++C LP LL P+ Sbjct: 6 TDHYSKFLFIWFGQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYG 65 Query: 70 GVTADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVS 129 G+ ADR +RR ++I D L +Y + + SV S F A Sbjct: 66 GILADRHDRRLMMILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYK 125 Query: 130 SSLLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLM 189 + + L+ + Q +A L Q+A S L++P L G++ ++ +K L++ F +++ + Sbjct: 126 ALITDLLPENQYAKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSI 185 Query: 190 SCGLR 194 +R Sbjct: 186 VIWIR 190 >NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe transport Length = 443 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 49/185 (26%), Positives = 91/185 (49%) Query: 10 TPSNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFA 69 T L + G+ S++GSG+ TF +G+++ + TG+ S+ ++C LP LL P+ Sbjct: 6 TDHYSKFLFIWFGQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYG 65 Query: 70 GVTADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVS 129 G+ ADR +RR ++I D L +Y + + SV S F A Sbjct: 66 GILADRHDRRLMMILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYK 125 Query: 130 SSLLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLM 189 + + L+ + Q +A L Q+A S L++P L G++ ++ +K L++ F +++ + Sbjct: 126 ALITDLLPENQYAKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSI 185 Query: 190 SCGLR 194 +R Sbjct: 186 VIWIR 190 >PAB0754 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 72/289 (24%), Positives = 126/289 (43%), Gaps = 23/289 (7%) Query: 10 TPSNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFA 69 T R+ LF G+ S+ G + + L++L TGSG ++ ++ L+PRIL+ P A Sbjct: 2 TKLGRDFWLFAVGRWISIAGWAIQDVAIPLYVLDKTGSGTMMSLFVMAELIPRILVNPIA 61 Query: 70 GVTADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVS 129 GV DR NR+ L++ D LL + V++S+ +F+ S Sbjct: 62 GVIGDRYNRKYLMVGFDLIRGVLLFFVLFFNLLELEQLLVIQ---VIMSIFGSFFAAGTS 118 Query: 130 SSLLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLM 189 LV +++ +A S+ Q+ + N+++PV+GG +Y L +K + +NA F S L Sbjct: 119 GMFPDLVPKDRLMQANSILQMGTQVINIVSPVIGGAIYGLGGIKAAIFINAISFFGSGLF 178 Query: 190 SCGLRFKPSTVTGVQTELPDSVPGGDARRVQTKVLAELRSSLAGGISYVFKKPVIRAXXX 249 +R++ G + A LR L G KP++ Sbjct: 179 EISIRYEWK---------------GKGEK-----FAILREFLDGIKLIKSSKPLLILGSL 218 Query: 250 XXXXXXXXXXSLNVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLL 298 +V P++ L LSS Q+G++ + G+L +L++ Sbjct: 219 SIILNALFSPLFAIVFPYIVRVVLRLSSIQFGSVQTALTLGMLLGNLII 267 >TM0342 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 413 Score = 86.7 bits (213), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 67/307 (21%), Positives = 126/307 (41%), Gaps = 26/307 (8%) Query: 3 MNDRVFWTPSNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPR 62 MN+ + W RN L G+ S+LGS + + LF+L LT SG + A+ V ++PR Sbjct: 1 MNEILHW---KRNFFLLALGRFVSILGSSVQAVAIPLFVLDLTHSGSAVALMTVAYMVPR 57 Query: 63 ILLAPFAGVTADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCST 122 IL P AG+ DR +R+ L++ D +L +Y S + +++ Sbjct: 58 ILFTPIAGIVGDRGDRKFLMVSMDFLRGFLICFLALLAFRNSITLPVLYVSQIAMAIMDN 117 Query: 123 FYGIAVSSSLLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAG 182 + I + +V + + RA S+ + +L P LGG++Y + + L+N Sbjct: 118 LFDIPTGAMFPDIVPQDFLMRANSIMGMVRIFPQILGPALGGVIYGFYGIAIVFLVNGVS 177 Query: 183 FAVSTLMSCGLRFKPSTVTGVQTELPDSVPGGDARRVQTK--VLAELRSSLAGGISYVFK 240 F +S + + + +R+V+ K +++ L G +++ Sbjct: 178 FVLSAISEIFIVY--------------------SRKVEKKPAKFSQILEDLKEGFLFIWN 217 Query: 241 KPVIRAXXXXXXXXXXXXXSL-NVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLLT 299 +IR L VV P+ + + S+ ++G + G L + L+ Sbjct: 218 HELIRTILIFALFLNLLISPLFMVVYPYTIREVMKFSAQEFGFLETSFTLGALLGNFLIA 277 Query: 300 VRRQKTS 306 K + Sbjct: 278 SYLSKKN 284 >PAB0724 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 52/187 (27%), Positives = 90/187 (48%), Gaps = 3/187 (1%) Query: 13 NRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFAGVT 72 NRN LF G+ S LG + + L++L +T S + ++ L+P I+ P AGV Sbjct: 2 NRNFWLFVAGRFISQLGWAIQEVALPLYVLDVTHSAKIMTLFVLADLIPSIITTPIAGVF 61 Query: 73 ADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVSSSL 132 DR +R+K+++ D LL V V++++ + F+ + Sbjct: 62 GDRYDRKKIMVGFDLIRGVLLFLVIAFNFLTIRCLLVVQ---VIMAIMNAFFSAGTGAMY 118 Query: 133 LQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLMSCG 192 LV+ E+++RA S+ A I L+ P +GG +Y +KL +L+NA F S L Sbjct: 119 PDLVKPEELERANSIVMSASIISRLVGPAIGGFVYGFGGIKLAILMNAISFFGSGLFEIF 178 Query: 193 LRFKPST 199 ++++ T Sbjct: 179 IKYEWKT 185 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,813,384 Number of extensions: 586174 Number of successful extensions: 2593 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2598 Number of HSP's successfully gapped: 14 Length of query: 322 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 216 Effective length of database: 26,153,027 Effective search space: 5649053832 Effective search space used: 5649053832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 116 (49.3 bits)