BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0247.0037.p
         (138 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

mll4699 [M] COG1970 Large-conductance mechanosensitive channel         98   5e-21
mlr5692 [M] COG1970 Large-conductance mechanosensitive channel         96   1e-20
mlr5747m [M] COG1970 Large-conductance mechanosensitive channel        94   5e-20
mlr4713 [M] COG1970 Large-conductance mechanosensitive channel         92   2e-19

>mll4699 [M] COG1970 Large-conductance mechanosensitive channel
          Length = 144

 Score = 97.8 bits (242), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 55/114 (48%), Positives = 74/114 (64%), Gaps = 14/114 (12%)

Query: 1   LEEIEMWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLK-- 58
           +EE  M +EF++F  KGNV+DLAV V+IGAAFGKIV SLV DIIMP++G + GG+D    
Sbjct: 1   MEETRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPIIGAIFGGLDFNNY 60

Query: 59  --GLSFAYG----------DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNRFKR 100
             GLS A             AV+ YG F+   ++F I+AF IF++VK VN  ++
Sbjct: 61  FVGLSSAVNATSLADARKQGAVLAYGSFITVALNFVILAFIIFLMVKAVNNLRK 114


>mlr5692 [M] COG1970 Large-conductance mechanosensitive channel
          Length = 157

 Score = 95.9 bits (237), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 55/113 (48%), Positives = 74/113 (65%), Gaps = 14/113 (12%)

Query: 2   EEIEMWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLK--- 58
           +E  M +EF++F  KGNV+DLAV V+IGAAFGKIV+SLV D+IMP+ G + GG+D     
Sbjct: 14  KEGRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVTSLVDDVIMPIFGAIFGGLDFNNYY 73

Query: 59  -GLSFAYG----------DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNRFKR 100
            GLS A             AV  YG F+ +V++F I+AF IF++VK VN  +R
Sbjct: 74  IGLSSAVNATSLAEAKKQGAVFAYGSFITAVLNFLILAFIIFLMVKAVNNLRR 126


>mlr5747m [M] COG1970 Large-conductance mechanosensitive channel
          Length = 140

 Score = 94.4 bits (233), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 55/109 (50%), Positives = 70/109 (64%), Gaps = 14/109 (12%)

Query: 6   MWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLK----GLS 61
           M +EF++F  KGNV+DLAV V+IGAAFGKIV SLV DIIMP+VG + GG+D      GLS
Sbjct: 1   MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPIVGAIFGGLDFNNYFLGLS 60

Query: 62  FAYG----------DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNRFKR 100
            A             AV  YG F+   ++F I+AF IF++VK VN  +R
Sbjct: 61  SAVNATSLADAKKQGAVFSYGSFITVALNFVILAFIIFLIVKAVNNLRR 109


>mlr4713 [M] COG1970 Large-conductance mechanosensitive channel
          Length = 140

 Score = 92.4 bits (228), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 54/109 (49%), Positives = 70/109 (64%), Gaps = 14/109 (12%)

Query: 6   MWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLK----GLS 61
           M +EF++F  KGNV+DLAV V+IGAAFGKIV SLV DIIMP++G + GG+D      GLS
Sbjct: 1   MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPVIGAIFGGLDFNNYFVGLS 60

Query: 62  FAYG----------DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNRFKR 100
            A             AV  YG F+   ++F I+AF IF++VK VN  +R
Sbjct: 61  SAVNATSLADAKKQGAVFAYGSFITVALNFVILAFIIFLMVKAVNNLRR 109


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.328    0.146    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 7,978,020
Number of extensions: 264554
Number of successful extensions: 932
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 928
Number of HSP's successfully gapped: 4
Length of query: 138
Length of database: 38,378,431
Length adjustment: 95
Effective length of query: 43
Effective length of database: 27,421,701
Effective search space: 1179133143
Effective search space used: 1179133143
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 110 (47.0 bits)