BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0036.p (366 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_138_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 134 3e-31 STM1828_1 [P] COG0861 Membrane protein TerC, possibly involved i... 118 1e-26 all0082_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 109 8e-24 XF0950_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 91 4e-18 aq_436 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 89 1e-17 >aq_138_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 134 bits (336), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 15/213 (7%), Positives = 37/213 (17%), Gaps = 9/213 (4%) Query: 148 KQPFVTLKSASTLDGKLATRTGDSKWISNGQAREIVHTLRHRHQGIMVGVNTVIADNPSL 207 + + S L T T + ++ + + I V + P Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 208 TTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQAPTIVVTTESADPVKRAALLDAGVQIV 267 E N + I + ++ D ++ + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALD--RKNIREALHDSLK 119 Query: 268 VSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGAMLENGLVDRVVLFFAPKIVGGGSA 327 L + A + L L + Sbjct: 120 RLQT---DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIR-Y 175 Query: 328 SPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCI 360 + + ++ Sbjct: 176 IGVSN---ETAFGVMRYLHLADKHDLPRIVTIQ 205 >STM1828_1 [P] COG0861 Membrane protein TerC, possibly involved in tellurium resistance Length = 144 Score = 118 bits (296), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 63/142 (44%), Positives = 85/142 (59%), Gaps = 4/142 (2%) Query: 7 EFYMSLALDMAERAQGQTGINPVVGCVVVKDGAMIGLGTHLQRGTGHAEVHALNMAAGKA 66 E +M A+++A++ G+ NP VG VVVKDG +I G H G HAE A+ A K Sbjct: 3 ETFMKRAIELAKKGLGRVNPNPPVGAVVVKDGRIIAEGFHPYFGGPHAERMAIESARKKG 62 Query: 67 Q---GSTAYVTLEPCSHYGKTPPCSQRLIDEGVARVVVACEDPNPQVAGRGITMLREQGI 123 + G+T VTLEPC H+GKTPPC+ +I+ G+ VV+ DPNP V+G G+ R GI Sbjct: 63 EDLRGATLIVTLEPCDHHGKTPPCTDLIIESGIKTVVIGTRDPNP-VSGNGVEKFRNHGI 121 Query: 124 EVEVGLLRSRALRLNEKFIKYI 145 EV G+L +L E FI Y+ Sbjct: 122 EVIEGVLEEEVKKLCEFFITYV 143 >all0082_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 211 Score = 109 bits (272), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 0/217 (0%), Positives = 0/217 (0%), Gaps = 9/217 (4%) Query: 148 KQPFVTLKSASTLDGKLATRTGDSKWISNGQAREIVHTLRHRHQGIMVGVNTVIADNPSL 207 K + + + + T + ++ N Sbjct: 3 KAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPA 62 Query: 208 TTRMEVPGL-NPVRIVIDSTLRIPLDSAVVRDGQAPTIVVTTESADPVKRAALLDAGVQI 266 G+ + L +++ + E + L + Sbjct: 63 AMLEL--GVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK 120 Query: 267 V-VSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGAMLENGLVDRVVLFFAPKIVGGG 325 G +E + + A V Sbjct: 121 TANVVLNT----AFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCH 176 Query: 326 SASPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCISG 362 + Sbjct: 177 HWLILHGRY- 212 >XF0950_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 90.5 bits (223), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 18/222 (8%), Positives = 36/222 (16%), Gaps = 28/222 (12%) Query: 149 QPFVTLKSASTLDGKLATRTGDSKWISNGQAREIVHTLRHRHQGIMVGVNTVIADNPSLT 208 + S L T T + ++ + + I V + P Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 209 TRM---EVPGLNPVRIVIDSTLRIPLDSAVVRDGQAPTIV-----VTTESADPVKRAAL- 259 L + S R+ Sbjct: 63 GLTETYVGNWLAKHGSREKLIIA-SKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 260 -LDAGVQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGAMLENGLVDRVVLFFA 318 D + L + L A Sbjct: 122 QTDYL-----------DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRA 170 Query: 319 PKIVGGGSASPGTFDFPGVEL--MQSAVTLEGLEVEVLGDNV 358 KI S + + + A + + + + Sbjct: 171 GKIR-YIGVSN---ETAFGVMRYLHLADKHDLPRIVTIQNPY 208 >aq_436 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 88.6 bits (218), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 12/181 (6%), Positives = 29/181 (16%), Gaps = 6/181 (3%) Query: 148 KQPFVTLKSASTLDGKLATRTGDSKW----ISNGQAREIVHTLRHRHQGIMVGVNTVIAD 203 + + S L T T + + + M V Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 204 NPSLTTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQAPTIVVTTESADPVKRAALLDAG 263 T + L + + + + + + + Sbjct: 62 QGLTETYV-GNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 264 VQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGAMLENGLVDRVVLFFAPKIVG 323 +Q +V L+ + V Sbjct: 121 LQTDYLD-LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 324 G 324 Sbjct: 180 N 180 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,793,177 Number of extensions: 1063740 Number of successful extensions: 2552 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2541 Number of HSP's successfully gapped: 15 Length of query: 366 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 258 Effective length of database: 25,922,359 Effective search space: 6687968622 Effective search space used: 6687968622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)