BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0026.p (400 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_dacB [M] COG1686 D-alanyl-D-alanine carboxypeptidase 260 2e-69 BH1573 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 252 7e-67 BH2877 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 202 7e-52 CAC2057 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 188 1e-47 BS_dacF [M] COG1686 D-alanyl-D-alanine carboxypeptidase 179 6e-45 >BS_dacB [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 382 Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 146/347 (42%), Positives = 218/347 (62%), Gaps = 19/347 (5%) Query: 33 NSTITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKN 92 ++ I A++A +ID ASGR+LY+ + IAS+TKIMTA++AIE+G + V V N Sbjct: 26 HAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLAIESGKMDQTVTVSAN 85 Query: 93 AFAKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEEL 152 A EGS+++L +G++++L+D++YGLMLRSGNDAA AIAEHVGGS +GFVY+MN KAE+L Sbjct: 86 AVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQL 145 Query: 153 GLQHTHFTNPHGLDA-EGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYK 211 G+++T F NPHGLD E HYS+A D+A+LT YAM +++I T++ A E + Sbjct: 146 GMKNTRFQNPHGLDDHENHYSTAYDMAILTKYAMKLKDYQKISGTKIYKA----ETMESV 201 Query: 212 WLNKNKMLR-LYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLD 270 W NKNK+L LY + G KTGYTK A R LVS+A++ G L+AVT+ND NDW+DH + + Sbjct: 202 WKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGIDLIAVTINDPNDWDDHMKMFN 261 Query: 271 YGFNHYPLKTLVERGE--GISGYSFVPARTF-----AYPLGQGEEARLVTKLELIRENKN 323 Y F HY + ++G+ + G +F ++ F Y L + E+ + L++ K Sbjct: 262 YVFEHYQTYLIAKKGDIPKLKG-TFYESKAFIKRDITYLLTEEEKENVKINTTLLKPKKA 320 Query: 324 TRRSSFGIK---GALVLQLGGKEIGRVPVYMPDRLPPEQSPYEKRFQ 367 + + I G + + I +VP+Y + Q P ++ F+ Sbjct: 321 WEKDASKIPDIVGHMEIMFNDATIAKVPIYYENE--RHQKPKKQFFE 365 >BH1573 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 382 Score = 252 bits (644), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 149/337 (44%), Positives = 213/337 (63%), Gaps = 16/337 (4%) Query: 40 ARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAKEGS 99 A+AA L++ ++GR+LY + IAS+TKIMTAL+AIE+G + VKV NA EGS Sbjct: 35 AQAAILMEQSTGRVLYEKDAHTSLRIASITKIMTALLAIESGRMDEWVKVSSNAEGTEGS 94 Query: 100 SLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQHTHF 159 SL+L+ GE+++L D++YGLMLRSGNDAA AIAEHVGGS +GFVY+MN KAEELG+ +T F Sbjct: 95 SLYLRAGEKIKLSDLVYGLMLRSGNDAAVAIAEHVGGSMDGFVYMMNQKAEELGMSNTVF 154 Query: 160 TNPHGLDA-EGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNKNKM 218 NPHGLD E HYSSA D+A+LT YAM+N +++I T+ DN + W NKN++ Sbjct: 155 QNPHGLDDHEDHYSSAYDMALLTRYAMNNETYQEISGTKTHRVDNGEQI--RVWKNKNRL 212 Query: 219 L-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFNHYP 277 L +LY+ + G KTGYTK+A R LVS+A++ L+ VTLN +DW+DH L ++ F+ + Sbjct: 213 LTQLYKYSTGGKTGYTKRAKRTLVSTASKDDLDLIVVTLNAPSDWHDHMNLFNWAFHTFG 272 Query: 278 LKTLVERGEGISGY--SFVPARTFA-----YPLGQGEEARLVTKLELIRENKNTRRSSFG 330 + L+E G+ + G F + +A YPL + E ++ ++ L R K+ RR Sbjct: 273 MTELLEEGK-VKGLKDEFYAGQVYASHDVVYPLTEEEREKVRKEITLYRPPKD-RREPLN 330 Query: 331 IK---GALVLQLGGKEIGRVPVYMPDRLPPEQSPYEK 364 + G + L G + P+Y P + + K Sbjct: 331 LPSPIGKMSFFLDGYVLTETPLYYKGTEPKPKGFWSK 367 >BH2877 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 395 Score = 202 bits (514), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 115/275 (41%), Positives = 167/275 (60%), Gaps = 8/275 (2%) Query: 39 HARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAKEG 98 ++ + L+D SG++LYS GD+ M AS+TKI+TAL+AIE GDL V V K A G Sbjct: 44 NSESVLLMDQLSGQVLYSEHGDKKMYPASITKIITALIAIEEGDLDDIVTVSKTATEVIG 103 Query: 99 SSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAE-ELGLQHT 157 + ++L +GEE+ L ++ GLM+ SGNDA TAIAEH G+EE F MN + ++G+++T Sbjct: 104 TRVYLVEGEEVTLRKLVQGLMINSGNDAGTAIAEHFDGNEEAFAERMNRFVQVKIGVENT 163 Query: 158 HFTNPHGLDAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNKNK 217 HFTNPHGL + HY++A D+A++T YAM N F+ + T K + E W+ N ++ Sbjct: 164 HFTNPHGLFDKEHYTTAYDMAMITKYAMENEKFRDVAST--KELEWIGEGWETTIYNHHR 221 Query: 218 MLRLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLN---DGNDWNDHAVLLDYGFN 274 +L Y G GVK GY ++ LV++A R QQL+AVTLN +ND LLDYGF Sbjct: 222 LLWDYPGTTGVKNGYVSQSGYTLVTTAERDDQQLIAVTLNAPGASFSYNDTMTLLDYGFE 281 Query: 275 HYPLKTLV--ERGEGISGYSFVPARTFAYPLGQGE 307 H+ + + ER G + A+ F + + + E Sbjct: 282 HFKTEKIKAGERFVDSDGKEYELAKDFYFSIPEDE 316 >CAC2057 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 351 Score = 188 bits (478), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 107/256 (41%), Positives = 148/256 (57%), Gaps = 2/256 (0%) Query: 31 AENSTITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVG 90 A+ + +AR A ID + +LY D + +AS TKIMT+LVAI+ G+L K + Sbjct: 22 AKADKLNLNARCAIAIDSNTKMILYEKNADMIVPMASTTKIMTSLVAIKYGNLDKKFTIS 81 Query: 91 KNAFAKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAE 150 K A + GS + K GEE+ L ++LYGLMLRSGNDAA IAE + G+ E F LMN A Sbjct: 82 KKAASIRGSKVGYKAGEEITLRELLYGLMLRSGNDAAICIAEGIAGNTEKFADLMNEYAY 141 Query: 151 ELGLQHTHFTNPHGLDAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDY 210 ++G ++HF PHGLD + HY +A DLA +T+ A N VF IV + K + + Sbjct: 142 KIGAFNSHFQTPHGLDKDLHYCTAYDLAFITSVAKENKVFNDIVSS--KDVGSGQYGFTR 199 Query: 211 KWLNKNKMLRLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLD 270 + N NK+L GADGVKTGYT A +CLVSS G ++ V LN W + + + Sbjct: 200 SYHNINKILYQIPGADGVKTGYTGNAGKCLVSSVKMNGHDVIFVVLNCTPRWKETKRMYE 259 Query: 271 YGFNHYPLKTLVERGE 286 Y +Y K +V +G+ Sbjct: 260 YVKKNYEYKKMVSKGD 275 >BS_dacF [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 389 Score = 179 bits (455), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 120/337 (35%), Positives = 184/337 (54%), Gaps = 23/337 (6%) Query: 33 NSTITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGD-----LRSKV 87 S + A++A LI+ +G++LY+ +E + AS+TKIMT L+ +E D + KV Sbjct: 30 TSELAHEAKSAVLIERDTGKVLYNKNSNERLAPASMTKIMTMLLIMEALDKGKIKMSDKV 89 Query: 88 KVGKNAFAKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNA 147 + ++A + GS +FL+ GEEM +++ML G+ + SGNDA+ A+AE + GSEE FV MN Sbjct: 90 RTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEFISGSEEEFVKKMNK 149 Query: 148 KAEELGLQHTHFTNPHGLDAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEP 207 KA+ELGL++T F NP GL EGHYSSA D+A++ + ++ I K D E Sbjct: 150 KAKELGLKNTSFKNPTGLTEEGHYSSAYDMAIMAKELLK---YESITKFTGTYEDYLREN 206 Query: 208 WDYK-WL-NKNKMLRLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDH 265 D K WL N N++++ Y G DGVKTGYT +A CL +SA +G + +AV + Sbjct: 207 TDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYCLTASAKKGNMRAIAVVFGASTPKERN 266 Query: 266 A---VLLDYGFNHYPLKTLVERGEGISGYSFVPARTFAYPLGQGEEARLVTKL-----EL 317 A +LD+ F+ Y L +R + ++ + L E ++TK ++ Sbjct: 267 AQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKGKQKFIELTTSEPISILTKKGEDMNDV 326 Query: 318 IRENKNTRRSSFGIK-----GALVLQLGGKEIGRVPV 349 +E K S I+ G LVL+ G+ + PV Sbjct: 327 KKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAESPV 363 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,104,465 Number of extensions: 1193403 Number of successful extensions: 2221 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 2073 Number of HSP's successfully gapped: 72 Length of query: 400 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 291 Effective length of database: 25,807,025 Effective search space: 7509844275 Effective search space used: 7509844275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)