BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0016.p (479 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2061 [T] COG0642 Signal transduction histidine kinase 331 1e-90 BS_resE_4 [T] COG0642 Signal transduction histidine kinase 301 1e-81 SA1322 [T] COG0642 Signal transduction histidine kinase 235 1e-61 lin2643 [T] COG0642 Signal transduction histidine kinase 199 7e-51 SA1515 [T] COG0642 Signal transduction histidine kinase 191 2e-48 >lin2061 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 331 bits (849), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 0/407 (0%), Positives = 0/407 (0%), Gaps = 13/407 (3%) Query: 73 PMQQVIEAADNIRRGEYGTRLTLVTSDEIGLLATSFNHMAEELEENIRSLNHEKGHLSSV 132 Sbjct: 202 261 Query: 133 LRSMSDAVLTFDIIGKIILTNPHGQALLERWNDLDWELENESELYPQGASDSEVPPPLRP 192 Sbjct: 262 ----------- 310 Query: 193 LFFSTLREGGDQRSQVHVRQGVWSVHMAPLYSEDTIRGVVAVLRDVTEEVKLEKMRRDFV 252 Sbjct: 311 370 Query: 253 ANVSHEIRTPLSMMQGYSEALLDGMASSPEESSELVQVIHDESLRMGRLVKDLLDLARME 312 Sbjct: 371 430 Query: 313 AGHTDMMKVPVDMGELLERVYRKFSVRAKERGLQLELKSGSDTLLLKAADEDKLEQVLTN 372 Sbjct: 431 - 489 Query: 373 LLDNAFRHTPADKCI-TILTSTAVLEGRRYLEIAIRDEGVGIPPEDLPFIFERFYKADKA 431 Sbjct: 490 549 Query: 432 RVRGESGGTGLGLAIVKNIVEAHHGTITASSRLGEGTEFLLRLPVEK 478 Sbjct: 550 596 >BS_resE_4 [T] COG0642 Signal transduction histidine kinase Length = 269 Score = 301 bits (772), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 154/267 (57%), Positives = 201/267 (75%), Gaps = 12/267 (4%) Query: 215 WSVHMAPLYSEDTIRGVVAVLRDVTEEVKLEKMRRDFVANVSHEIRTPLSMMQGYSEALL 274 W + M+PLY+E +RG VAVLRD+TEE +L+K+R DF+ANVSHE+RTP+SM+QGYSEA++ Sbjct: 12 WVLLMSPLYAESHVRGAVAVLRDMTEERRLDKLREDFIANVSHELRTPISMLQGYSEAIV 71 Query: 275 DGMASSPEESSELVQVIHDESLRMGRLVKDLLDLARMEAGHTDMMKVPVDMGELLERVYR 334 D +ASS E+ E+ Q+I+DESLRMGRLV DLLDLARME+GHT + +++ E LE++ R Sbjct: 72 DDIASSEEDRKEIAQIIYDESLRMGRLVNDLLDLARMESGHTGLHYEKINVNEFLEKIIR 131 Query: 335 KFSVRAKERGLQLELKSGSDTLLLKAA---DEDKLEQVLTNLLDNAFRHTPADKCITILT 391 KFS AKE+ + L+ D L + DEDK+EQV TNL+DNA RHT A ++I + Sbjct: 132 KFSGVAKEKNIALD----HDISLTEEEFMFDEDKMEQVFTNLIDNALRHTSAGGSVSI-S 186 Query: 392 STAVLEGRRYLEIAIRDEGVGIPPEDLPFIFERFYKADKARVRGESGGTGLGLAIVKNIV 451 +V +G L+I I+D G GIP EDLPFIFERFYKADKAR RG + GTGLGLAIVKNIV Sbjct: 187 VHSVKDG---LKIDIKDSGSGIPEEDLPFIFERFYKADKARTRGRA-GTGLGLAIVKNIV 242 Query: 452 EAHHGTITASSRLGEGTEFLLRLPVEK 478 EAH+G+IT SR+ +GT F +P ++ Sbjct: 243 EAHNGSITVHSRIDKGTTFSFYIPTKR 269 >SA1322 [T] COG0642 Signal transduction histidine kinase Length = 588 Score = 235 bits (599), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 142/404 (35%), Positives = 232/404 (57%), Gaps = 24/404 (5%) Query: 72 QPMQQVIEAADNIRRGEYGTRLTLVTSDEIGLLATSFNHMAEELEENIRSLNHEKGHLSS 131 +P++++ + A + G+Y + ++ T DEIG L+ +FN M+ E+EE++ +L+ K S Sbjct: 206 KPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDS 265 Query: 132 VLRSMSDAVLTFDIIGKIILTNPHGQALLERWNDLDWELENESELYPQGASDSEVPPPLR 191 ++ SM + VL + +IIL+N ND+ ++ +++ + + + Sbjct: 266 LINSMVEGVLGINESRQIILSNKMA-------NDIMDNIDEDAKAF--------LLRQIE 310 Query: 192 PLFFSTLREGGDQRSQVHVRQGVWSVHMAPLYSEDTIRGVVAVLRDVTEEVKLEKMRRDF 251 F S E D +++ R V + + GVV +RD+T E L++M++DF Sbjct: 311 DTFKSKQTEMRDL--EMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDF 368 Query: 252 VANVSHEIRTPLSMMQGYSEALLDGMASSPEESSELVQVIHDESLRMGRLVKDLLDLARM 311 +ANVSHE+RTP+S++QGY+E+++DG+ + P+E E + V+ DES R+ RLV +LL++ARM Sbjct: 369 IANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAVVLDESKRLNRLVNELLNVARM 428 Query: 312 EAGHTDMMKVPVDMGELLERVYRKFSVRAKERGLQLELKSGSDTLLLKAADEDKLEQVLT 371 +A + K + LL+++ K+ +A + GL + + + D D+++QVLT Sbjct: 429 DAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVW--SYDMDRMDQVLT 486 Query: 372 NLLDNAFRHTPADKCITILTSTAVLEGRRYLEIAIRDEGVGIPPEDLPFIFERFYKADKA 431 NL+DNA R+T I I E Y I+D G GI PE L +F+RFYK D A Sbjct: 487 NLIDNASRYTKPGDEIAITCDENESEDILY----IKDTGTGIAPEHLQQVFDRFYKVDAA 542 Query: 432 RVRGESGGTGLGLAIVKNIVEAHHGTITASSRLGEGTEFLLRLP 475 R RG+ GTGLGL I K I+E H G+I S LG+GT F+++LP Sbjct: 543 RTRGKQ-GTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLP 585 >lin2643 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 199 bits (506), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 0/408 (0%), Positives = 0/408 (0%), Gaps = 20/408 (4%) Query: 72 QPMQQVIEAADNIRRGEYGTRLTLVTSDEIGLLATSFNHMAEELEENIRSLNHEKGHLSS 131 Sbjct: 197 256 Query: 132 VLRSMSDAVLTFDIIGKIILTNPHGQALLERWNDLDWELENESELYPQGASDSEVPPPLR 191 Sbjct: 257 ----------- 305 Query: 192 PLFFSTLREGGDQRSQVHV---RQGVWSVHMAPLYSED-TIRGVVAVLRDVTEEVKLEKM 247 Sbjct: 306 365 Query: 248 RRDFVANVSHEIRTPLSMMQGYSEALLDGMASSPEESSELVQVIHDESLRMGRLVKDLLD 307 Sbjct: 366 425 Query: 308 LARMEAGHTDMMKVPVDMGELLERVYRKFSVRAKERGLQLELKSGSDTLLLKAADEDKLE 367 Sbjct: 426 485 Query: 368 QVLTNLLDNAFRHTPADKCITILTSTAVLEGRRYLEIAIRDEGVGIPPEDLPFIFERFYK 427 Sbjct: 486 ---- 541 Query: 428 ADKARVRGESGGTGLGLAIVKNIVEAHHGTITASSRLGEGTEFLLRLP 475 Sbjct: 542 - 588 >SA1515 [T] COG0642 Signal transduction histidine kinase Length = 554 Score = 191 bits (486), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 131/411 (31%), Positives = 223/411 (54%), Gaps = 36/411 (8%) Query: 72 QPMQQVIEAADNIRRGEYGTRLTLVTSDEIGLLATSFNHMAEELEENIRSLNHEK----G 127 +P+Q+V A + G Y R+ E L S N +A L++ LN+E+ Sbjct: 159 RPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQK----LNNEQKIQSN 214 Query: 128 HLSSVLRSMSDAVLTFDIIGKIILTNPHGQALLERWNDLDWELENESELYPQGASDSEVP 187 L + + ++ A+L D GKI++ N +A E++N + +E ++ G ++E+ Sbjct: 215 RLKTTIENIPSAILMIDRNGKIVVAN---KAYYEQFN-ISHNIE---QVGYHGYVNTEIE 267 Query: 188 PPLRPLF------FSTLREGGDQRSQVHVRQGVWSVHMAPLY--SEDTIRGVVAVLRDVT 239 + F + L +Q VH + + + P+ S+ +++G++ V+ D+T Sbjct: 268 QLILESFKVEKPIYEQLEVAINQ---VHAK--YFDISCVPILTRSQKSLQGILVVMHDIT 322 Query: 240 EEVKLEKMRRDFVANVSHEIRTPLSMMQGYSEALLDGMASSPEESSELVQVIHDESLRMG 299 +LE +RR+FVANVSHE++TP++ ++G++E L+DG + E + +I ES R+ Sbjct: 323 NLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIE 382 Query: 300 RLVKDLLDLARMEAGHTDMMKVPVDMGELLERVYRKFSVRAKERGLQLELKSGSDTLLLK 359 LV DLLDL+ +E HT++ +++ +L R+ +A ++ + + D ++ Sbjct: 383 SLVTDLLDLSHIEQ-HTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIV-- 439 Query: 360 AADEDKLEQVLTNLLDNAFRHTPADKCITILTSTAVLEGRRYLEIAIRDEGVGIPPEDLP 419 A E K+ QV+TNLL NA ++ D I + V + ++D G+GI ED Sbjct: 440 KAQESKIAQVITNLLTNAINYSYEDGDINV----RVYRDDFRVIFEVQDFGIGIKLEDQQ 495 Query: 420 FIFERFYKADKARVRGESGGTGLGLAIVKNIVEAHHGTITASSRLGEGTEF 470 IFERFY+ DKAR R +SGGTGLGL+I K+IVEAH G I +S++G+G+ F Sbjct: 496 RIFERFYRVDKARSR-DSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTF 545 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,838,601 Number of extensions: 1346337 Number of successful extensions: 15525 Number of sequences better than 1.0e-05: 286 Number of HSP's gapped: 14892 Number of HSP's successfully gapped: 289 Length of query: 479 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 369 Effective length of database: 25,691,691 Effective search space: 9480233979 Effective search space used: 9480233979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)