BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0015.p (482 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1430 [K] COG1609 Transcriptional regulators 536 e-152 TM1430 [C] COG0554 Glycerol kinase 536 e-152 PA3582 [C] COG0554 Glycerol kinase 516 e-146 SSO2133 [C] COG0554 Glycerol kinase 471 e-132 SSO1600 [C] COG0554 Glycerol kinase 469 e-132 >STM1430 [K] COG1609 Transcriptional regulators Length = 341 Score = 536 bits (1380), Expect = e-152, Method: Compositional matrix adjust. Identities = 0/463 (0%), Positives = 0/463 (0%), Gaps = 1/463 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 + + + + + P + + +I + I Sbjct: 18 VSHVINKTRFVAEETRNAVWAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAAYFAEI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 E T + +R K + ++ + + Sbjct: 78 IEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLVMCSEYPEPLLSMLEEY 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 + ++ G ++ + D+ + N R Sbjct: 138 RHIPMVVMDWGEAKADFTDTVIDNAFAGGYMAGRYLVERGHRDIGVIPGPLERNTGAGRL 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 + + G +G + + Sbjct: 198 AGFMKAMEEALINVPDNWIVQGDFEPESGYHAMQQILSQSHRPTAVFCGGDIMAMGALCA 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 A + G + V ++ + + + V E Sbjct: 258 ADEMGLRVPQDVSVIGYDNVRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREESQ 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 Sbjct: 318 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 Sbjct: 378 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRY 462 Sbjct: 438 480 >TM1430 [C] COG0554 Glycerol kinase Length = 482 Score = 536 bits (1380), Expect = e-152, Method: Compositional matrix adjust. Identities = 256/463 (55%), Positives = 326/463 (70%), Gaps = 1/463 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ +VS+ E RQ +PRPGWVEHDP EIWE+Q+ A+ AI E+G +I+AIGI Sbjct: 18 VFDEKGNVVSKVNKEFRQIYPRPGWVEHDPVEIWESQIEVAKKAIEEAGIKPEDIAAIGI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C++LK +G ++ I +TGLV+DAYFS T Sbjct: 78 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDELKEKGYSEFIRERTGLVIDAYFSGT 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 138 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGRVHVTDYSNASRTMIFNIHKLDW 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPRA+LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 198 DDEILELLNIPRAMLPQVMPSSHVYGYTAKDIFGVEIPIAGDAGDQQAALFGQACFQPGM 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 258 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 318 KIISNAAETEELATKVPDNGGVFFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q+RDV+ MEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 378 LESIAYQTRDVVEVMEKDSDIKVETLRVDGGAVVNNFLMQFQADILGVPVERPVVNETTA 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRY 462 G W ++E++ S + ++ F PSM +EERER Y Sbjct: 438 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLY 480 >PA3582 [C] COG0554 Glycerol kinase Length = 505 Score = 516 bits (1328), Expect = e-146, Method: Compositional matrix adjust. Identities = 264/481 (54%), Positives = 323/481 (67%), Gaps = 2/481 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++A +VSQ Q E Q +P+ GWVEHDP EIW TQ + A++++ +++AIGI Sbjct: 24 VFDRDANVVSQAQREFAQFYPQAGWVEHDPMEIWATQSSTLVEALAQASIEHDQVAAIGI 83 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWD+ +G+PI+ AIVWQ RR+A C QLK GL D I TGLV D YFS T Sbjct: 84 TNQRETTVVWDRHSGRPIHNAIVWQCRRSAAICAQLKRDGLEDYIRETTGLVTDPYFSGT 143 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 KL WILD+V GAR RA G+LL GTID WLIWKLT G VH TD TNASRTML+N+H R W Sbjct: 144 KLKWILDNVEGARERARNGDLLFGTIDTWLIWKLTEGKVHVTDYTNASRTMLFNIHSRDW 203 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGV-EIPIRSVLGDQQAALFGHTCLEAG 239 D IPR++LPEVR S +G A G E+PI + GDQQAALFG C+E G Sbjct: 204 DARMLEVLDIPRSMLPEVRNSSEVYGNARIGGVGGGELPIAGIAGDQQAALFGQMCVEPG 263 Query: 240 SAKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEG 299 AKNTYGTGCF+LM+TG +AV S+HGLLTT+A G E+ YALEG+VF G+ VQWL + Sbjct: 264 QAKNTYGTGCFLLMHTGDKAVKSTHGLLTTIACGPRGEVGYALEGAVFNGGSTVQWLRDE 323 Query: 300 LGVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRA 359 L VI DSE A PAFTGLGAPYWD YARGAVFGLTRG A HL+RA Sbjct: 324 LKVINDSFDSEYFATKVKDSNGVYLVPAFTGLGAPYWDPYARGAVFGLTRGVKADHLIRA 383 Query: 360 TLESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXX 419 TLES+A+Q+RDV+ AM++DAG L LRVDGGAV N+ LMQFQADILG+ V R + E Sbjct: 384 TLESIAYQTRDVLDAMQRDAGERLRALRVDGGAVANNFLMQFQADILGTRVERPVMRETT 443 Query: 420 XXXXXXXXXXXSGIWNR-EQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W+ ++L+S E++F P + RE+ Y W+ AV RT GW+ Sbjct: 444 ALGAAYLAGLACGFWSSLDELKSKAVIERVFEPECDEPRREKLYAGWKKAVERTRGWDDG 503 Query: 479 E 479 E Sbjct: 504 E 504 >SSO2133 [C] COG0554 Glycerol kinase Length = 499 Score = 471 bits (1212), Expect = e-132, Method: Compositional matrix adjust. Identities = 233/482 (48%), Positives = 311/482 (64%), Gaps = 6/482 (1%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +++Q+ ++ GQY+ Q +P PG+VEH+PDEIW Q+ A + A+ ++ + +++ IG+ Sbjct: 20 IYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAIKEAMKKAKIESRQVAGIGV 79 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET ++WD +GKPIY AIVWQDRRT+ + LK I KTGL+ D YFS + Sbjct: 80 TNQRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYFG-MIKDKTGLIPDPYFSGS 138 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD++P R++AE+GE+ GTID +LIWKLT G +H TD +NASRTML+N+++ W Sbjct: 139 KIKWILDNLPNVRSKAEKGEIKFGTIDTYLIWKLTNGKIHVTDYSNASRTMLFNINKLEW 198 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D IP +ILPEVR S +G +E G IPI GDQQAALFG + G Sbjct: 199 DREILELLKIPESILPEVRPSSDIYGY--TEVLGSSIPISGDAGDQQAALFGQVAYDMGE 256 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGM-GDELYYALEGSVFVAGAAVQWLHEG 299 K+TYGTG FILMN G+ + S + LLTT+AWG+ + YALEGS+F+ GAAVQW +G Sbjct: 257 VKSTYGTGSFILMNIGSNPIFSEN-LLTTIAWGLESKRVTYALEGSIFITGAAVQWFRDG 315 Query: 300 LGVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRA 359 L I+ D E A PAF GLGAPYWD YARG + G+TRGTT H+ RA Sbjct: 316 LRAIDASDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARA 375 Query: 360 TLESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXX 419 LES+A+Q+RDVI MEK++G + L+VDGG +++LLMQFQADILG V R E Sbjct: 376 ILESIAYQNRDVIEIMEKESGTKINILKVDGGGAKDNLLMQFQADILGIRVVRPKVMETA 435 Query: 420 XXXXXXXXXXXSGIWNR-EQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 WN +L+ +K F PS+ EERERRY+AW++AV R++GWEK Sbjct: 436 SMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYNAWKEAVKRSLGWEKS 495 Query: 479 EG 480 G Sbjct: 496 LG 497 >SSO1600 [C] COG0554 Glycerol kinase Length = 501 Score = 469 bits (1208), Expect = e-132, Method: Compositional matrix adjust. Identities = 242/482 (50%), Positives = 309/482 (64%), Gaps = 8/482 (1%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 LFD + +V+ GQYE Q +P+PG+VEHDP+EIWE Q+ A + AIS+ A +I AIGI Sbjct: 23 LFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQMLAVKKAISKID--AKQIVAIGI 80 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET ++WD +GKP+Y AIVWQDRRT+ + LK I KTGLV D YFSA+ Sbjct: 81 TNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANYFK-MIKDKTGLVPDPYFSAS 139 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+VP R +AE+GE+ GT+D +LIW+LT G H TD +NASRTML+N+++ Sbjct: 140 KIKWILDNVPNVREKAERGEIKFGTLDTYLIWRLTNGKAHVTDYSNASRTMLFNINKLEC 199 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D IP +ILPEV+ S +G SE G IPI GDQQAALFG G Sbjct: 200 DREILELLKIPESILPEVKPSSEIYGY--SEALGNLIPISGDAGDQQAALFGQVAFNVGE 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGM-GDELYYALEGSVFVAGAAVQWLHEG 299 K TYGTG FILMN G + S + LLTT+AWG+ ++ YALEGS+F+ GAAVQW +G Sbjct: 258 IKATYGTGSFILMNIGNNPIRSEN-LLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDG 316 Query: 300 LGVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRA 359 L I+ + E A PAF GLGAPYWD YARG + G+TRGTT H+ RA Sbjct: 317 LRAIDVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARA 376 Query: 360 TLESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXX 419 LES+A+Q+RDVI M+K++G+ + L+VDGGA +++LLMQFQADILG +V R E Sbjct: 377 ILESMAYQTRDVIEVMQKESGISINSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETT 436 Query: 420 XXXXXXXXXXXSGIWNR-EQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G+WN E+L S K +K F PSM E+R Y W++AV R MGW K Sbjct: 437 SMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALYSGWKEAVKRAMGWAKV 496 Query: 479 EG 480 G Sbjct: 497 VG 498 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,453,371 Number of extensions: 1373864 Number of successful extensions: 3139 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 3032 Number of HSP's successfully gapped: 66 Length of query: 482 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 372 Effective length of database: 25,691,691 Effective search space: 9557309052 Effective search space used: 9557309052 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)