BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0011.p (510 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA3792 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 235 1e-61 MJ0277 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 224 3e-58 MK0531 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 214 4e-55 SP0445 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 207 3e-53 YPO0539 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 206 1e-52 >MA3792 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Length = 211 Score = 235 bits (600), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 0/513 (0%), Positives = 0/513 (0%), Gaps = 32/513 (6%) Query: 1 MVDYGIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVL 60 D + + + AA L + T G + + Sbjct: 42 ATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHN 101 Query: 61 FITGHQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSG 120 +P + A + + F + + Sbjct: 102 GEVPEDRAALEALPGVGR-----KTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNV 156 Query: 121 QRGPVHLCIPFDVQTEVLEECRIVIPEREHL-----VSAGNLERVLSAINE---SSHPLI 172 ++ L + + ++ + E Sbjct: 157 EQVEEKLLKVVPAEFK---VDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKE 213 Query: 173 IAGKGVNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLN 232 Sbjct: 214 273 Query: 233 RSDVFIVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRD---N 289 Sbjct: 274 - 332 Query: 290 LAFYLQNFDSAAI------KKHETETDVHYAEELPVLPKLSLASVMDALSDLIPYNNTIF 343 Sbjct: 333 ----- 387 Query: 344 VDDGSHGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFM 403 Sbjct: 388 447 Query: 404 MLGTEINTAVCKDIPVIFIVVNNMQLDMALKGMEK-TTGRIDGTIFEVPMDAVKFAESLG 462 Sbjct: 448 507 Query: 463 ATGYRCETLEQFTSAIHAAVASNRVAVIELLTD 495 Sbjct: 508 540 >MJ0277 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Length = 591 Score = 224 bits (571), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 165/522 (31%), Positives = 251/522 (48%), Gaps = 34/522 (6%) Query: 12 RHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGHQSIKEL 71 RHE ++ A G+A +G+ GV TSGPG TNL+T A A A++ PV+ +TG K + Sbjct: 45 RHEQAAAHAADGFARASGEAGVCVSTSGPGATNLVTGIATAYADSSPVIALTGQVPTKLI 104 Query: 72 GIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPVHLCIPF 131 G Q+ D +F P T ++ +++ + F A IA +G+ GPVH+ +P Sbjct: 105 GNDAFQE-----IDALGLFMPITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPK 159 Query: 132 DVQTEVLEECRIVIPEREHLVSAG--------NLERVLSAINESSHPLIIAGKGVNRSGA 183 DVQ ++ + IP + L +++ I ES P+I+AG GV SGA Sbjct: 160 DVQDGEIDIEKYPIPAKVDLPGYKPKTVGHPLQIKKAAKLIAESERPVILAGGGVIISGA 219 Query: 184 HEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVFIVLGSR 243 EELL+LAE IPV T+ GKG DHPL G +G+ G K + DV I +G R Sbjct: 220 SEELLRLAEFVKIPVCTTLMGKGCFPEDHPLALGMVGMHGTKAANYAVTECDVLIAIGCR 279 Query: 244 LSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNFDSAAIK 303 SD +++ +I D+DP +GK + ++ I + GD ++ L L + IK Sbjct: 280 FSDRVTGDIRYFAPEAKIIHIDIDPAEIGKNVRAD-IPIVGDAKNVLRDLLAALIALEIK 338 Query: 304 KHETETDVHYA-----------EELPVLPKLSLASVMDALSDL-IPYNNTIFVDD-GSHG 350 ET + Y ++ P+ P+ + +M+ L+++ NTI D G + Sbjct: 339 DKETWLERIYELKKLSIPMMDFDDKPIKPQRFVKDLMEVLNEIDSKLKNTIITTDVGQNQ 398 Query: 351 FNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEIN 410 A ++K K P S + MG AIGAKVA P ++ ITGDG F+M E+ Sbjct: 399 MWMAHFFKTKMPRSFLASGGLGTMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQELA 458 Query: 411 TAVCKDIPVIFIVVNNMQLDMALKGMEKTTGRIDGTIF--EVPMDAVKFAESLGATGYRC 468 T DIPV+ + +N L M + G+ + E P D VK AES G R Sbjct: 459 TISEYDIPVVICIFDNRTLGMVYQWQNLYYGQRQSEVHLGESP-DFVKLAESYGVKADRI 517 Query: 469 ETLEQFTSAIHAAVASNRVAVIELLTDRTE----VPPTAHRT 506 + ++ + A+ SN +++++ D E VPP T Sbjct: 518 ISPDEIKEKLKEAILSNEPYLLDIVIDPAEALPMVPPGGRLT 559 >MK0531 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Length = 565 Score = 214 bits (544), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 161/519 (31%), Positives = 246/519 (47%), Gaps = 29/519 (5%) Query: 3 DYGIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFI 62 + IE I RHE + A GYA GK GV TSGPG TNL+T A A ++ PV+ I Sbjct: 41 EVSIEHILVRHEQGAAHAADGYARVKGKPGVCMATSGPGATNLVTGIATAYMDSSPVIAI 100 Query: 63 TGHQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQR 122 TG +G Q+ D +F P T ++ + + + + A IA++G+ Sbjct: 101 TGQVPTTMIGKDAFQE-----VDAVGVFMPITKHNYQIGKPEEIPEVVKEAFKIAITGRP 155 Query: 123 GPVHLCIPFDVQ-TEV-LEECRIVIPEREHLVSAG---NLERVLSAINESSHPLIIAGKG 177 GPVH+ +P DVQ EV +E + V E ++V G ++R + E+ P+I+AG G Sbjct: 156 GPVHIDVPKDVQEAEVDVEIPKTVEVEGLNVVKRGHPVQIKRAAELLAEAERPVILAGGG 215 Query: 178 VNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVF 237 S A EL++LAE PV T+ GKGA DHPL G G+ G K L DV Sbjct: 216 CVISNATRELIELAELLGAPVATTLMGKGAFPEDHPLALGMAGMHGTKAANYALTECDVL 275 Query: 238 IVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGD----LRDNLA-- 291 + +G R SD T + +I D+DP +GK + + + + GD LRD + Sbjct: 276 LAVGCRFSDRTTGDPSGFAPEAKIIHIDIDPAEIGKNIPVD-VPIVGDAKLVLRDLIKEL 334 Query: 292 ---FYLQNFDSAAIKKHETETDVHYAEE-----LPVLPKLSLASVMDALSDLIPYNNTIF 343 YL+ + E + +V E + P+ + + +AL D + + Sbjct: 335 KRRKYLRERKRWGERIEELKAEVEMPPESTESDQRISPRELVRVLHEALKD---RDYILT 391 Query: 344 VDDGSHGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFM 403 D G + A+++ V++P + MG + A+GAKVA+PE+T++ + GDG F+ Sbjct: 392 TDVGQNQMWMARYFPVEEPRRFISSGGLGTMGFGLPAALGAKVAAPEKTVVAVVGDGGFL 451 Query: 404 MLGTEINTAVCKDIPVIFIVVNNMQLDMALKGMEK-TTGRIDGTIFEVPMDAVKFAESLG 462 M E+ TAV DI V V++N L M + R+ + + D VK ES G Sbjct: 452 MTAQELATAVDNDIEVKVFVMDNRLLGMVAQWQRLFYDERLSESKLDEKTDIVKLTESYG 511 Query: 463 ATGYRCETLEQFTSAIHAAVASNRVAVIELLTDRTEVPP 501 A G E + SA+ A + V+++ D E+ P Sbjct: 512 AAGITVEEPSELESAVEEAFETPGTVVVDVFVDPEEIIP 550 >SP0445 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Length = 566 Score = 207 bits (527), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 146/508 (28%), Positives = 244/508 (48%), Gaps = 25/508 (4%) Query: 5 GIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITG 64 GI I GRHE EA GYA TGKLGVA TSGPG TN +T A A ++++P+L TG Sbjct: 48 GIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPLLVFTG 107 Query: 65 HQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGP 124 + +G Q+ AD+ + P T Y+ V I A+ IA +G+ GP Sbjct: 108 QVARAGIGKDAFQE-----ADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRPGP 162 Query: 125 VHLCIPFDV---QTEVLEECRIVIPEREHLVSAGNLE--RVLSAINESSHPLIIAGKGVN 179 V + +P D+ +T+ + + +P + + +++ ++L ++++ P+++AG G++ Sbjct: 163 VVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGIS 222 Query: 180 RSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVFIV 239 + A EL + AE + IPVVTS G+G IA HPL+ G G+ G + +D I Sbjct: 223 YAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMIS 282 Query: 240 LGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYL----- 294 +GSR D N K + D+DP +GKI++++ I V GD + L L Sbjct: 283 IGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISAD-IPVVGDAKKALQMLLAEPTV 341 Query: 295 -QNFDS--AAIKKHETETDVHYAEELPVLPKLSLASVMDALSDLIPYNNTIFVDDGSHGF 351 N + + K + + +E V P+ +V++ + +L + + D G H Sbjct: 342 HNNTEKWIEKVTKDKNRVRSYDKKERVVQPQ----AVIERIGELTNGDAIVVTDVGQHQM 397 Query: 352 NAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINT 411 A++Y + +V MG I AIGAK+A+P++ ++ GDG F M E+ Sbjct: 398 WTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAI 457 Query: 412 AVCKDIPVIFIVVNNMQLDMALKGMEK-TTGRIDGTIFEVPMDAVKFAESLGATGYRCET 470 +P+ +++NN L M + E GR ++F+ D A++ G Y+ + Sbjct: 458 LNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDN 517 Query: 471 LEQFTSAIHAAVASNRVAVIELLTDRTE 498 E + + + +IE+ R E Sbjct: 518 PETLAQDLE-VITEDVPMLIEVDISRKE 544 >YPO0539 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Length = 575 Score = 206 bits (523), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 151/521 (28%), Positives = 250/521 (47%), Gaps = 37/521 (7%) Query: 5 GIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITG 64 GI+ + RHE + A GYA TG++GV TSGPG TN +T A A +++P++ ++G Sbjct: 42 GIDHVLVRHEQGAVHMADGYARATGEVGVVLVTSGPGATNAITGIATAYMDSVPMVVLSG 101 Query: 65 HQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGP 124 +G Q+ D+ + RP +S LV+R + + A +A +G+ GP Sbjct: 102 QVPSSLIGFDAFQE-----CDMVGISRPVVKHSFLVKRTEDIPMVLKKAFYLASTGRPGP 156 Query: 125 VHLCIPFDVQTEVLEECRIVIPEREHLVS--------AGNLERVLSAINESSHPLIIAGK 176 V + +P D+ ++ PE+ L S G ++R L I + P++ G Sbjct: 157 VVIDLPKDIVGPAVK-MPYAYPEQVSLRSYNPTVQGHRGQIKRALQTIMAAKRPIMYVGG 215 Query: 177 GVNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDV 236 G + HEELL LAE N+PV +S G G+ H G +G+ G ++ +D+ Sbjct: 216 GAINAACHEELLILAEKLNLPVTSSLMGLGSFPGTHRQSVGMLGMHGTFEANMAMHNTDL 275 Query: 237 FIVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYL-- 294 +G R D T NL T++ D+DPT + K +T++ I + GD + L L Sbjct: 276 IFAVGVRFDDRTTNNLAKYCPDATVLHIDIDPTSISKTVTAD-IPIVGDAKQVLTQMLDL 334 Query: 295 -------QNFDS-----AAIKKHETETDVHYAEELPVLPKLSLASVMDALSDLIPYNNTI 342 Q+FDS +I++ T + Y ++ K+ +V++AL L + + Sbjct: 335 LSQVGCTQDFDSLRDWWQSIEQWRTRNCLGYNKD---SGKIKPQAVIEALHRLTKGDAYV 391 Query: 343 FVDDGSHGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCF 402 D G H AA +Y KP + MG + A+G K+A P ET++C+TGDG Sbjct: 392 TSDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPNETVVCVTGDGSI 451 Query: 403 MMLGTEINTAVCKDIPVIFIVVNNMQLDMALKGMEKT-TGRIDGTIFEVPMDAVKFAESL 461 M E++TA+ ++PV+ + +NN L M + + +GR + + D VK AE+ Sbjct: 452 QMNIQELSTALQYNLPVLVLNLNNRYLGMVKQWQDMIYSGRHSQSYMDSLPDFVKLAEAY 511 Query: 462 GATGYRCETLEQFTSAIHAAVA----SNRVAVIELLTDRTE 498 G G T ++ S + A+ +NR+ +++ D TE Sbjct: 512 GHIGVAINTPDELESKLADALTQLSETNRLVFVDVTVDETE 552 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,656,443 Number of extensions: 1617638 Number of successful extensions: 3453 Number of sequences better than 1.0e-05: 142 Number of HSP's gapped: 3091 Number of HSP's successfully gapped: 160 Length of query: 510 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 399 Effective length of database: 25,576,357 Effective search space: 10204966443 Effective search space used: 10204966443 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)