BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0009.p (416 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mcpC [NT] COG0840 Methyl-accepting chemotaxis protein 131 2e-30 BH2275 [NT] COG0840 Methyl-accepting chemotaxis protein 130 4e-30 PAB1308 [NT] COG0840 Methyl-accepting chemotaxis protein 127 5e-29 BH0876_2 [NT] COG0840 Methyl-accepting chemotaxis protein 124 2e-28 BH0565 [NT] COG0840 Methyl-accepting chemotaxis protein 124 4e-28 >BS_mcpC [NT] COG0840 Methyl-accepting chemotaxis protein Length = 654 Score = 131 bits (330), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 115/373 (30%), Positives = 182/373 (48%), Gaps = 30/373 (8%) Query: 58 FIERTLTSSFDDISNVTHTIAKGDFTNRADENGS--MGDISRSFNTMIDKLKKILTDASQ 115 F+ +T+T + T ++ GD T RA+ +G ++R FN M++ +K+++ Sbjct: 294 FLAKTITGPIQQLIVKTKAVSAGDLTVRAESKSKDEVGILTRDFNLMVENMKEMV----- 348 Query: 116 ITRQVMDASRGIEDKNQNLKIVMA-------QVASSSNELALGANEISVDIADMTESIKD 168 QV +S + D ++ L V A Q+A + E+A GA+E + ++ + E + Sbjct: 349 --EQVRLSSGKVSDTSEQLTAVAAETNERSGQIAKAIEEVAAGASEQASEVETINEKSES 406 Query: 169 IETKVSNYTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQA---TQKVADTIEAL 225 + TK+ + RS + + KG ++ + + K+ +A T+K + L Sbjct: 407 LSTKIRQIAEEAGGIKERSKSSEDASYKGLHAL---GQLLMKSNEANMETKKEETMLLDL 463 Query: 226 SQNARGITMITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTAST 285 + I + I+ I++QTNLL+LN SIEAARAGE GRGFAVVA EVRKLAE+S ST Sbjct: 464 ENQTKNIEEVVTAISNISDQTNLLALNRSIEAARAGESGRGFAVVADEVRKLAEQSALST 523 Query: 286 KEVFSLVRSIENDIKQA----IENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQI 341 K + V+ I+ + K+A +E +N+E QN I ET + I +Q + + I Sbjct: 524 KHISETVKLIQLETKEASHAMVEASRMNDE----QNSAIHETGEVLNTITAEMQSLVQGI 579 Query: 342 ASFSAESDLMLESALKISGAIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMT 401 AE M E L IS AI++IS +EQ+ L V TE + Sbjct: 580 DHIYAEIQRMSEEQLAISEAIQSISAISQESAAAAEEVNASTDEQLVTLDKVKHSTETLK 639 Query: 402 QAVFQLQKTIHIF 414 A + TI F Sbjct: 640 HASQDVINTIRKF 652 >BH2275 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 671 Score = 130 bits (327), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 103/373 (27%), Positives = 177/373 (47%), Gaps = 28/373 (7%) Query: 58 FIERTLTSSFDDISNVTHTIAKGDFTN--RADENGSMGDISRSFNTMIDKLKKILTDASQ 115 + R++T + T+A GD T RA N +G ++ SF M+ +L+ I+T Sbjct: 305 LVARSITKPISRLGTHVGTVASGDLTVDVRAAGNDEVGQLTESFKEMLVQLRGIITTVKG 364 Query: 116 ITRQVMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTESIKDIETKVSN 175 T V +A+ +L + + +SS E+AL ++++ E + + + ++ Sbjct: 365 STAHVQEAA-------DHLTAISEETIASSEEIALAVRDVAIGSTKQVEHLDEAKRQIDQ 417 Query: 176 YTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEA----------- 224 ++M +L + E G S +T+ G + + T+EA Sbjct: 418 LARQMEQM------SLSMTEVGSLSNETRLAGQSGSEKVQHLKTKTLEANTVFDQMEDGL 471 Query: 225 --LSQNARGITMITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEEST 282 LS + I + TI ++EQTNLL+LNASIEAARAGEHG+GFAVVA EVR+LAE+S+ Sbjct: 472 LKLSDHIMEINEVIHTINHLSEQTNLLALNASIEAARAGEHGKGFAVVANEVRRLAEQSS 531 Query: 283 ASTKEVFSLVRSIENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIA 342 T V + I N KQ I ++ ++ Q++ + ET N F + ++ + E +A Sbjct: 532 HETVRVGDTITGIVNRSKQVIVDMKQARSILSEQSDSVDETENTFLHMASLIKQMNETLA 591 Query: 343 SFSAESDLMLESALKISGAIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMTQ 402 S + + E + IE + +EQ+ ALQ + E E++ + Sbjct: 592 SVQEDFGTLAEQNSQFVKRIEAVVEVAEQAAATSEEVNASTDEQVTALQRITEAAEQLQE 651 Query: 403 AVFQLQKTIHIFK 415 A +L +++ F+ Sbjct: 652 ASHELNQSVRQFQ 664 >PAB1308 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 435 Score = 127 bits (318), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 96/285 (33%), Positives = 158/285 (55%), Gaps = 23/285 (8%) Query: 60 ERTLTSSFDDISNVTHTIAKGDFTNRADENGSMGDISRSFNTMIDKLKKILTDASQITRQ 119 E+ L D+I + IA+GD + E G MG R I+ L+K + SQ+ Sbjct: 65 EKQLQREIDEIVKILDRIAQGDLSVEDKEIGGMGAKVRQG---IEDLRKSI---SQLVLN 118 Query: 120 VMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVD-------IADMTESIKDIETK 172 + +A+ + + + +K + QVA S ++A N++S++ I+ MT++++ I Sbjct: 119 IKNAAIDVRNHTKVMKENIEQVADSVQQVAEAINQVSIEAQREQENISKMTDTMRYIHD- 177 Query: 173 VSNYTSSTKEMNRRSVHTL-----ELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALSQ 227 +S T ST E S+ + E EKGRQ+ D E R +K+ +T+ +++ Sbjct: 178 ISKETVSTMEEFEASMREMAQLAKEGGEKGRQAADQIEEISR----MMEKIEETVRGVAE 233 Query: 228 NARGITMITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTKE 287 + I IT I+ I+EQTNLL+LNA+IEAARAGE G+GFAVVA E+RKLAEES + ++ Sbjct: 234 MGKSIENITNVISSISEQTNLLALNAAIEAARAGEAGKGFAVVADEIRKLAEESKKAAED 293 Query: 288 VFSLVRSIENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQ 332 + L++ I + I +++E EVV+ E+I E+ + Q+ + Sbjct: 294 IRELIKQIGDKIGESVEVTQQGAEVVKTSTEVIKESVSYLTQVAE 338 >BH0876_2 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 359 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 104/372 (27%), Positives = 182/372 (48%), Gaps = 23/372 (6%) Query: 52 TFPLSRFIERTLTSSFDDISNVTHTIAKGDFT-------NRADENGSMGDISRSFNTMID 104 T PL+R ++ V +A GD T NR DE +G ++ SF M + Sbjct: 4 TKPLAR------------LTKVARHVADGDLTTERVKIKNR-DE---IGQLNESFEAMKE 47 Query: 105 KLKKILTDASQITRQVMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTE 164 L+ ++ Q QV +S + + Q S+ ++ GA+ + + Sbjct: 48 NLRGLIQRILQNADQVAVSSEQLSASAEQTSSATEQTTSAIESISRGADMQTSAAQTSVK 107 Query: 165 SIKDIETKVSNYTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEA 224 +++++ V+ T K++ + TL +G V E M+ ++ + I+ Sbjct: 108 TLEEMNQIVTEMTDGAKQIESNANLTLTYANEGDTLVKETVENMQSIDESVKGSDQAIQT 167 Query: 225 LSQNARGITMITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTAS 284 LS+ + I I I IAEQTNLL+LNA+IEAARAGEHG+GFAVVA+EVRKLAEES+ S Sbjct: 168 LSKRSEDIVEILDVIQSIAEQTNLLALNAAIEAARAGEHGKGFAVVAEEVRKLAEESSQS 227 Query: 285 TKEVFSLVRSIENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASF 344 TK++ L+ + + ++E + + +E V V + +T+ F IV S+Q +T Q+ + Sbjct: 228 TKKIHDLIDEMVKETNNSVEKMGLVKEEVSVGLKTAAQTSEKFHSIVASIQGVTSQVEAM 287 Query: 345 SAESDLMLESALKISGAIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMTQAV 404 + + + E + +++ +++N++ E + ++Q V ++T+ Sbjct: 288 NRVTREVAEKSTEVATSVQNMASVAEESAEQSSSVSAVAEETLASMQEVTASANQLTEMA 347 Query: 405 FQLQKTIHIFKF 416 LQ+ I FK Sbjct: 348 ETLQEAIKQFKL 359 >BH0565 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 401 Score = 124 bits (310), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 97/312 (31%), Positives = 165/312 (52%), Gaps = 31/312 (9%) Query: 69 DISNVTHTIAKGDFTNRADENGSMGDISRSFNTMIDKLKKILTDASQITRQVMDASRGIE 128 +I+N I G N+ DE S + FN +I+ + S++ + + S+ + Sbjct: 37 EITNGMKEIVAGKLVNKMDEKSSNHAV---FNQLIEFV-------SRVREKFLLFSKNVH 86 Query: 129 DKNQNL-----------KIVMA---QVASSSNELALGANEISVDIADMTESIKDIETKVS 174 +K +NL IV A +V N+ + E ++ + +MT++I+D+ + Sbjct: 87 EKGENLAEIGEYATEKADIVRAAIDEVGRGLNKQLVATEENAMSMEEMTQAIEDLSQR-- 144 Query: 175 NYTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALSQNARGITM 234 + E++ S TL+L ++G + + E M++ Q D I L + + I M Sbjct: 145 -----SNEISELSNTTLDLTQEGSEKLKDSLEKMKQLNQTINTTYDAINKLGEKSHEIGM 199 Query: 235 ITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTKEVFSLVRS 294 I K IT I+EQ NLL+LNASIEAARAGEHG+GFAVVA EVRKLAE+S S+ EV ++V++ Sbjct: 200 IVKVITGISEQINLLALNASIEAARAGEHGKGFAVVADEVRKLAEQSRQSSSEVSNIVKN 259 Query: 295 IENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASFSAESDLMLES 354 I+ + + ++ E + N I+E +F +I+ + + I E + SA ++ + + Sbjct: 260 IQEETGIVVGSMQKGTEEFEDTNTTIVEVGAMFEKILTATRTIAENNGNSSASTEELSAA 319 Query: 355 ALKISGAIENIS 366 + +I IE I+ Sbjct: 320 SQQIMTTIEEIA 331 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.126 0.323 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,077,572 Number of extensions: 657982 Number of successful extensions: 4968 Number of sequences better than 1.0e-05: 806 Number of HSP's gapped: 4648 Number of HSP's successfully gapped: 809 Length of query: 416 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 307 Effective length of database: 25,807,025 Effective search space: 7922756675 Effective search space used: 7922756675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 117 (49.7 bits)