BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0007.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0410 [IQR] COG1028 Dehydrogenases with different specificities... 207 2e-53 CAC2626 [IQR] COG1028 Dehydrogenases with different specificitie... 86 5e-17 Ta0191 [IQR] COG1028 Dehydrogenases with different specificities... 84 3e-16 SA2260 [IQR] COG1028 Dehydrogenases with different specificities... 83 6e-16 hdhA [IQR] COG1028 Dehydrogenases with different specificities (... 82 1e-15 >BH0410 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 302 Score = 207 bits (527), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 114/280 (40%), Positives = 164/280 (58%), Gaps = 12/280 (4%) Query: 7 MTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRS-RGLSQEDRRKGTIDSVLDEI 65 M L GKVALV G +RGAGRGIA+EL AGA VY+TGR+ R E R TI+ + + Sbjct: 1 MKPLIGKVALVAGATRGAGRGIAVELGAAGATVYVTGRTTRERRSEYNRPETIEETAELV 60 Query: 66 RAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPTD 125 AGG G AV DH + EA++ +I EQG+LD+LVN++WGG + + P W+ D Sbjct: 61 TNAGGTGIAVPVDHLDPQKVEALVSQIEREQGKLDVLVNDIWGGELLTEWNAPVWQHSLD 120 Query: 126 HWSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIH----TTYWDHYKYIDNFYYDLAKN 181 M + L+T+++A+PL+ +R G+++ T ++ Y YYDLAK Sbjct: 121 DGLRMLRLAIDTHLITSHFALPLLLKSR---GLVVEMTDGTAEYNREHYRQPLYYDLAKT 177 Query: 182 ALVRMAFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQDVA----ELQTSEST 237 +++RMA+GL+ EL + + L+PGWMR+E++L+ E +W+D SES Sbjct: 178 SVLRMAWGLAQELQPHECTAVALTPGWMRSEIMLDHFEVTERNWRDATIKEPHFVISESP 237 Query: 238 AYIGRAVVRLAADPEVLAMTGIPQQVGSLAEKYGFTDVDG 277 ++GRAV LA+DP V G G LA+ YGFTD+DG Sbjct: 238 RFVGRAVAALASDPNVSRWNGQSLSSGQLAQAYGFTDLDG 277 >CAC2626 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 86.3 bits (212), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 76/247 (30%), Positives = 116/247 (46%), Gaps = 18/247 (7%) Query: 6 MMTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRSRGLSQEDRRKGTIDSVLDEI 65 M +L GKVAL+TG SRG G+ IA E+AK G V I R + KG +D+ L I Sbjct: 1 MFEDLVGKVALITGSSRGIGKSIAEEMAKMGIEVVINYR-------NDEKGALDT-LKSI 52 Query: 66 RAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKP--FWELP 123 + +GG E +CD T SE + + I S+ G++D+LVNN SK F ++ Sbjct: 53 KNSGGIAEVYKCDITSYSEVKNMFEFIISKFGKIDLLVNNAG-------ISKIGLFIDMK 105 Query: 124 TDHWSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTTYWDHYKYIDNFYYDLAKNAL 183 + ++ + D R AV M R+ G II ++ W + Y AK A+ Sbjct: 106 ENDYNEIMDNDFRGVFNCTSLAVKYMLE-RKSGSIINISSIWGNVGASCEVLYSAAKGAV 164 Query: 184 VRMAFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQDVAELQTSESTAYIGRA 243 L ELA I V +SPG + TE+ N ++ ++ + T+ I + Sbjct: 165 NSFTKALGKELAPSGIRVNAISPGVIDTEMNGSFSNDEKNDLENEIPMCRFGKTSEIAKI 224 Query: 244 VVRLAAD 250 + LA++ Sbjct: 225 AIFLASE 231 >Ta0191 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 268 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 68/215 (31%), Positives = 103/215 (47%), Gaps = 19/215 (8%) Query: 2 RGAVMMTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRSRGLSQEDRRKGTIDSV 61 RG+ + NL GK+ALVTG S G G IA +A+AGA V + R S +DR D++ Sbjct: 5 RGSRIAINLTGKIALVTGSSSGLGAAIAKYMARAGAKVAVHYR----SGKDRA----DAI 56 Query: 62 LDEIRAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWE 121 +DEI+ GG A D ++ + + + I S+ G +DILVNN +R + E Sbjct: 57 VDEIKNDGGFAMAFYGDVSKKEDVQKLFSEIDSKLGTVDILVNNAGIDGKRELVG----E 112 Query: 122 LPTDHWSHMFDAGVRAQLMTNYYAV--PLMRSNREGGGIIIHTTYWDHYKYIDNFY-YDL 178 D W + + LM YY + R + G+II+ T Y + Y Sbjct: 113 DDPDDWEKV----IEVNLMGPYYCAREAVKRMKPKKSGVIINITSVHEYVPWSGYTAYSS 168 Query: 179 AKNALVRMAFGLSVELAADKIAVIPLSPGWMRTEL 213 AK L L+ EL+ I V+ ++PG ++T + Sbjct: 169 AKAGLSMFTKALAQELSDYNIRVVAIAPGAIKTPI 203 >SA2260 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 272 Score = 82.8 bits (203), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 57/217 (26%), Positives = 104/217 (47%), Gaps = 16/217 (7%) Query: 7 MTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRSRGLSQEDRRKGTIDSVLDEIR 66 M L+ KVA+VTG S G G+ A+ LA+ GAYV + +S+ +D+I+ Sbjct: 1 MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE----------TVDKIK 50 Query: 67 AAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPTDH 126 + G +A D + + + + I + GR+D+L NN N A+ E P D Sbjct: 51 SNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDN----AAGRIHEYPIDV 106 Query: 127 WSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTTYWDHYKYIDNFYYDLAKNALVRM 186 + + + +R ++ +PLM + +GG I+ +++ + + Y+ AK A++ Sbjct: 107 YDKIMNVDMRGTILMTKMMLPLMMN--QGGSIVNTSSFSGQAEDLYRSGYNAAKGAVINF 164 Query: 187 AFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEE 223 +++E D I ++PG + T LV + T E+ Sbjct: 165 TKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSED 201 >hdhA [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 82.0 bits (201), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 0/214 (0%), Positives = 0/214 (0%), Gaps = 19/214 (8%) Query: 10 LQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRSRGLSQEDRRKGTIDSVLDEIRAAG 69 + R + + AY R L Sbjct: 9 RDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTA---------LRAATIELGPLVRGLPHL 59 Query: 70 GEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPTDHWSH 129 D + I G + +L + + Sbjct: 60 RTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEG-----NALMQ-DLEEEGYLV 113 Query: 130 MFDAGVRAQLMTNYYAVPLMRSNREGGGIIIH-TTYWDHYKYIDNFYYDLAKNALVRMAF 188 + + + +YY + + I Y + Sbjct: 114 GLEKAKFVERLAHYYCEINVLHP--FRVGSGLAQRIFFEQLAIHAGYQLSWQGIEKEAWN 171 Query: 189 GLSVELAADKIAVIPLSPGWMRTELVLESLNTDE 222 + A + + + + +E Sbjct: 172 QANQSGAMGDLTALQMIFSKVVSE- 204 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,144,053 Number of extensions: 905452 Number of successful extensions: 2480 Number of sequences better than 1.0e-05: 188 Number of HSP's gapped: 2314 Number of HSP's successfully gapped: 188 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)