BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0006.p (530 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA0592 [HE] COG0111 Phosphoglycerate dehydrogenase and related d... 488 e-137 BH1602 [HE] COG0111 Phosphoglycerate dehydrogenase and related d... 488 e-137 SA1545 [HE] COG0111 Phosphoglycerate dehydrogenase and related d... 486 e-137 BS_serA [HE] COG0111 Phosphoglycerate dehydrogenase and related ... 473 e-133 sll1908 [HE] COG0111 Phosphoglycerate dehydrogenase and related ... 466 e-131 >MA0592 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 523 Score = 488 bits (1255), Expect = e-137, Method: Compositional matrix adjust. Identities = 252/528 (47%), Positives = 357/528 (67%), Gaps = 5/528 (0%) Query: 2 FKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKIIA 61 KVLVSD +S+ G++ L + TVD TGLSEDEL IGEYD L++RS T VT+++I Sbjct: 1 MKVLVSDSLSNEGLEILKE--HFTVDVNTGLSEDELVEKIGEYDALVIRSGTQVTQRVIE 58 Query: 62 AGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQAY 121 A LK++GRAGVGVDN+ ++AAT+ G++V NAP+GN I+ EH MMMA++R+IPQA Sbjct: 59 AADNLKIVGRAGVGVDNVDVDAATKKGIIVANAPEGNMISAAEHTIGMMMAMSRNIPQAN 118 Query: 122 AKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAEK 181 A W R F+GVE++GKTLG++G+GRIGSEVAKRA MN++ YDPF++ RA + Sbjct: 119 ASLKGREWKRNKFMGVEVKGKTLGIIGLGRIGSEVAKRASGLEMNLMGYDPFISEKRAME 178 Query: 182 LEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMAL 241 L VKLA+V++I + AD+ITVHTPL ETR+++ QF++MK G+RI+NCARGG+I+E AL Sbjct: 179 LGVKLATVNEISKEADYITVHTPLIKETRNILDDEQFDLMKSGVRILNCARGGIINEAAL 238 Query: 242 VEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQVL 301 V A++SG V GAA DVF +EPP P L+ +IVTPHLGAST EAQ NVAID++++V+ Sbjct: 239 VRALESGKVGGAALDVFVEEPPFG-SPLLNFDNVIVTPHLGASTQEAQVNVAIDIAKEVV 297 Query: 302 HILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLSD 361 +L NA+NIP V P M L PY L E +G A QL ++ + Y G++S Sbjct: 298 SVLTGGLAKNAINIPSVKPEAMAVLAPYIRLAELMGKIAGQLVDSNYEKVEIGYNGEISG 357 Query: 362 VDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTLKA 421 DT+PLT +KG+ G VN VN+ LAK+R + VV +K+ + +++ +++ L + Sbjct: 358 KDTRPLTVSALKGLLEMALGAGVNYVNAPALAKSRQIAVVESKSESAEEYSSTVSIKLSS 417 Query: 422 EPDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQNDV 481 + +LVAGT++ G +IV +++ VDI P G I H +KP +IG +LG+N++ Sbjct: 418 -GETAKLVAGTVV-GDEPKIVSIDEDRVDIFPAGRMIFAKHINKPNVIGPCCMVLGKNNI 475 Query: 482 NIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVL 529 NI+ MQVGR VGG +M+L VD V +L ++ +P I A+ + L Sbjct: 476 NISGMQVGRSEVGGVTMMVLNVDTDVSDAILDEVRQVPGILNAKLVTL 523 >BH1602 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 540 Score = 488 bits (1255), Expect = e-137, Method: Compositional matrix adjust. Identities = 255/532 (47%), Positives = 354/532 (66%), Gaps = 11/532 (2%) Query: 1 MFKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKII 60 ++++LVSD +S+ G+ L++ V ++ D+L EYD LLVRS TTVTE+++ Sbjct: 17 IYRILVSDAMSEQGLSPLLNDPRVECVQQNVADADDLH----EYDALLVRSATTVTEELM 72 Query: 61 AAGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQA 120 + LK+I RAGVGVDNI ++AAT+HG+VV+NAPDGNTI+T EH FAM+ +L R IPQA Sbjct: 73 EKMSSLKIIARAGVGVDNIDIDAATKHGIVVVNAPDGNTISTAEHTFAMICSLLRKIPQA 132 Query: 121 YAKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAE 180 A +G W RK F G ELRGKTLG++G GRIGS++AKRAKAF M +DPFLT RAE Sbjct: 133 NASIKAGEWKRKAFQGTELRGKTLGIIGFGRIGSQIAKRAKAFEMTPYVFDPFLTKARAE 192 Query: 181 KLEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMA 240 KL V +AS+DD+++ AD ITVHTPLT ET+ ++ KKG+ ++NCARGG+IDE A Sbjct: 193 KLGVTVASLDDVLQVADIITVHTPLTKETKGLLGMKNIGKTKKGVFLINCARGGIIDEEA 252 Query: 241 LVEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQV 300 L + G +AGAA DVFE+EP AD L+ +I TPH+ AST EAQ NVA VS++V Sbjct: 253 LKHYLREGHIAGAALDVFEEEPV-ADEELLAFDNVIATPHIAASTKEAQLNVAEQVSQEV 311 Query: 301 LHILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLS 360 LH L P N++N+P ++ V K++PY+ L +K+G SQ ++EI V Y G+++ Sbjct: 312 LHFLEGNPVSNSINLPTLSKEVYEKVKPYYELTKKMGFLLSQCMKTPVQEIEVRYGGNVA 371 Query: 361 DVDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKAS-KTKGFTNLITVTL 419 D++T TR ++ G + VN VN+ +AK R ++ +S +T G+ N+I + Sbjct: 372 DIETSITTRSLMAGFLSPRVDAGVNDVNAGLIAKERGISFTEKHSSNQTFGYANIIHAVV 431 Query: 420 KAEPDEERL--VAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLG 477 EER V GT ++ YG RIV++N F VD PEGH I I HND+PG+IG +G LL Sbjct: 432 HG---EERTFEVHGTFIKEYGPRIVKINGFNVDFVPEGHLIYIQHNDRPGVIGKMGQLLA 488 Query: 478 QNDVNIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVL 529 +++VNIA+MQVGR+ GG AIM++ VDK +V+ L + EI+ A+ I L Sbjct: 489 EHNVNIATMQVGRQEEGGDAIMMVAVDKVATDEVIEALKAVDEIHFADRIEL 540 >SA1545 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 534 Score = 486 bits (1252), Expect = e-137, Method: Compositional matrix adjust. Identities = 248/522 (47%), Positives = 348/522 (66%), Gaps = 3/522 (0%) Query: 2 FKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKIIA 61 F VLV+DPIS GI+ L+D VD +TGLSE+ L II Y L+VRSQTTVTE II Sbjct: 4 FNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIPSYHALIVRSQTTVTENIIN 63 Query: 62 AGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQAY 121 A LKVI RAGVGVDNI + AAT G++VINAPDGNTI+ EH+ AM++++AR+IPQA+ Sbjct: 64 AADSLKVIARAGVGVDNININAATLKGILVINAPDGNTISATEHSLAMLLSMARNIPQAH 123 Query: 122 AKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAEK 181 + W+R F G EL KTLGV+G GRIG VAKRA++FGM ILA+DP+LT ++A+ Sbjct: 124 QSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAKRAQSFGMKILAFDPYLTDEKAKS 183 Query: 182 LEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMAL 241 L + A+VD+I + +DF+T+HTPLTP+T+ +I+ F K ++I+N ARGG+IDE AL Sbjct: 184 LSITKATVDEIAQHSDFVTLHTPLTPKTKGLINADFFAKAKPSLQIINVARGGIIDEKAL 243 Query: 242 VEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQVL 301 ++A+D G ++ AA DVFE EP D P ++H KIIVTPHLGASTVEAQE VAI VS +++ Sbjct: 244 IKALDEGQISRAAIDVFEHEPA-TDSPLVAHDKIIVTPHLGASTVEAQEKVAISVSNEII 302 Query: 302 HILRNEPFINAVNIPPVAPS-VMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLS 360 IL + +AVN P + S + + ++ + L + +G A QL A I + Y GDL+ Sbjct: 303 EILIDGTVTHAVNAPKMDLSNIDDTVKSFINLSQTVGELAIQLMYNAPSSIKITYGGDLA 362 Query: 361 DVDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTLK 420 +D+ LTR I+ + G +VNI+N++ L + V + + GF+N + V L Sbjct: 363 SIDSSLLTRTIITHILKDDLGPEVNIINALMLLNQQQVTLNIENNKAETGFSNYLEVELS 422 Query: 421 AEPDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQND 480 + D + V ++ G+G RIV++N F VD+ P +QIV HND PG++G G LLG+ + Sbjct: 423 NDSDSVK-VGASVFTGFGPRIVRINNFSVDLKPNQYQIVSYHNDTPGMVGKTGALLGKYN 481 Query: 481 VNIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEIN 522 +NIASM +GR GG A+M+L+VD+ V ++ +L + E N Sbjct: 482 INIASMTLGRTEAGGDALMILSVDQPVSNNIIDELKQVGEYN 523 >BS_serA [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 410 Score = 473 bits (1217), Expect = e-133, Method: Compositional matrix adjust. Identities = 0/523 (0%), Positives = 0/523 (0%), Gaps = 9/523 (1%) Query: 1 MFKVLVSDPISDLGIQQLMDASDVT-VDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKI 59 M KV + + + + + + + + ++ Sbjct: 1 MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKG----ALDDEQLKESIRDAHF 56 Query: 60 IAAGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQ 119 I ++ + + + G ++ + Sbjct: 57 IGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVFNAPFSNTRSVAELV 116 Query: 120 AYAKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRA 179 + + G + + + Sbjct: 117 IGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYGHIGTQLGILAESLGMY 176 Query: 180 EKLEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEM 239 + + + + Sbjct: 177 VYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTKNMMGAKEISLMKPGSLLI 236 Query: 240 ALVEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQ 299 I A + A + + E + Sbjct: 237 NASRGTVVDIPALCDALASKHLAG-AAIDVFPTEPATNSDPFTSPLCEFDNVLLTPHIGG 295 Query: 300 VLHILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDL 359 + + + L L + Sbjct: 296 STQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPLHGGRRLMHIHENRPGVLTALN 355 Query: 360 SDVDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTL 419 Sbjct: 356 -- 413 Query: 420 KAEPDEERL-VAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQ 478 Sbjct: 414 473 Query: 479 NDVNIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEI 521 Sbjct: 474 516 >sll1908 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 554 Score = 466 bits (1199), Expect = e-131, Method: Compositional matrix adjust. Identities = 255/528 (48%), Positives = 343/528 (64%), Gaps = 10/528 (1%) Query: 1 MFKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKII 60 M KVLVSD I +GI L + V D KTGLSE E+ I+ EYD +++RS T VTEKII Sbjct: 29 MAKVLVSDSIDQVGIDILKQVAQV--DVKTGLSEAEIIDIVPEYDAIMLRSATKVTEKII 86 Query: 61 AAGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQA 120 AG++LK+IGRAGVGVDNI + AAT+ G+VV+N+P+GNTI EHA AMMMALARHIP A Sbjct: 87 QAGSQLKIIGRAGVGVDNIDVPAATRQGIVVVNSPEGNTIAAAEHALAMMMALARHIPDA 146 Query: 121 YAKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAE 180 W+RK F+G E+ KTLGV+G+G+IGS VA AKA GM +LAYDPF++ +RA+ Sbjct: 147 NKSVKESKWERKQFIGTEVYKKTLGVVGLGKIGSHVAGVAKAMGMKLLAYDPFISQERAD 206 Query: 181 KLEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMA 240 ++ L +D + ADFIT+H P TPET ++I+ MK RI+NC+RGG+IDE A Sbjct: 207 QIGCTLVDLDLLFSEADFITLHIPKTPETANLINAETLAKMKPTARIINCSRGGIIDEEA 266 Query: 241 LVEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQV 300 LV AI++ + GAA DVF +E P + +I+TPHLGAST EAQ NVA+DV+EQ+ Sbjct: 267 LVTAIETAQIGGAALDVFAQE-PLGESRLREFSNVILTPHLGASTEEAQVNVAVDVAEQI 325 Query: 301 LHILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLS 360 +L P +AVNIP + P VM KL+PY L E LG+ QL GG I + V GDL+ Sbjct: 326 RDVLLGLPARSAVNIPGLTPDVMEKLRPYLKLAETLGTLVGQLAGGRIDRLTVCLQGDLA 385 Query: 361 D-VDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTL 419 + ++QPL +KG+ ++ VN VN+ AK R + V+ TK + + ++ ++ L Sbjct: 386 EYTNSQPLVVAAIKGLLSQALRERVNYVNAAIEAKERGIRVIETKDASVRDYSG--SLHL 443 Query: 420 KAEPDE-ERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQ 478 KA E G LL RI V++FP+++ P + + H D PGIIG +G+LLG Sbjct: 444 KATGTMGEHSATGALLSNGEIRITDVDEFPINVPPNNYMLFTLHRDMPGIIGKIGSLLGS 503 Query: 479 NDVNIASMQVGRKIVGGAAIMLLTVDKAVPQQVL---VKLAGLPEINT 523 +VNIASMQVGRKIV G AIM L++D +P +L K+AG+ + T Sbjct: 504 FNVNIASMQVGRKIVRGDAIMALSLDDPLPDGLLSEITKVAGIRDAYT 551 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,037,088 Number of extensions: 1709035 Number of successful extensions: 4663 Number of sequences better than 1.0e-05: 174 Number of HSP's gapped: 4328 Number of HSP's successfully gapped: 174 Length of query: 530 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 419 Effective length of database: 25,576,357 Effective search space: 10716493583 Effective search space used: 10716493583 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)